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The map label for this gene is purF [H]

Identifier: 17546695

GI number: 17546695

Start: 2150306

End: 2151841

Strand: Reverse

Name: purF [H]

Synonym: RSc1976

Alternate gene names: 17546695

Gene position: 2151841-2150306 (Counterclockwise)

Preceding gene: 17546696

Following gene: 17546691

Centisome position: 57.9

GC content: 66.8

Gene sequence:

>1536_bases
ATGTGCGGTATCGTTGGGGTGGTTTCGGCCACGCCCGTCAATCAGCTGATCTACGACAGCCTGCTGCTGCTGCAGCACCG
GGGGCAGGACGCGGCGGGCATTGCCACCGCGGCCGGCAGCACCTTCCACATGCACAAGGCCAACGGCATGGTGCGCGACG
TGTTCCGCACGCGCAACATGCGCGGCCTGCCGGGCACCATCGGCATCGGCCAGGTGCGCTATCCGACCGCCGGCTCCGCC
AGCGAGGAAGAGGCCCAGCCGTTCTATGTCAGCGCACCGTTCGGCATCGTGCTGGCTCACAACGGCAACCTGACCAACTC
CGAGCAGCTGCGCGAAGAGATGTTCCGGCGCGACCGCCGCCACATCAACACGCATTCCGACTCCGAAGTGCTGCTCAACG
TGCTGGCCGACGAACTGCAGCGCGCCAGCAACGACATCGCGTTGTCGCCCGAGGCCATCTTCAAGGCGGTGGCCGGCATG
CATCGCCGCGTGCGCGGCTCGTACGCCATCGTGTCGCAGATCGCCGGCTACGGCATGCTGGCCGTGCGCGACCCGTTCGG
CATCCGTCCGCTGGCGCTGGGCAGCCAGGAAACGCCGGACGGCGTCGAGTGGATGGTGGCCTCCGAATCGGTGGCGCTGG
AAGGCATCGGCTTCAAGTTCGAGCGCGATGTGGCGCCGGGCGAAGCCATCTTCGTCGACCTCGAAGGCCAGCTGCACACC
AAGCAGTGCGCGGATCATCCGGTCCTGACGCCCTGCATCTTCGAATACGTCTATCTGGCGCGGCCGGACTCGCGCATGGA
CGGCGCGTCCGTGTACGACGCGCGCCTGCGCATGGGCGATTACCTGGCCGAGAAGATCAAGCGCGAGGTGACCGACCCGA
TCGACGTGGTGATGCCGATCCCCGATTCGAGCCGGCCGGCGGCCATGCAGGTCGCCAACTGCCTGGGCGTGCCGTACCGG
GAAGGCTTCTTCAAGAACCGCTACGTGGGCCGCACCTTCATCATGCCGGGCCAGGCGGTGCGCAAGAAATCGGTGCGCCA
GAAGCTGAACGCCATGGCGATCGAGTTCAAGGGCAAGAACGTGCTGATCGTCGACGATTCCATCGTGCGCGGCACCACGT
CGTCGGAGATCGTGCAGATGGCGCGCGATGCGGGCGCGAAGAAGGTGATCTTCGCCTCGGCGGCGCCGCCGGTGAAGTTC
CCCAACGTGTACGGCATCGACATGCCCACGCGCAGCGAACTGGTCGCGCACGGTCGCACGCACGACGAGATCGCCCGGAT
CATCGGCGCCGACCAGCTCGTCTACCAGGACGTGGAAGACATGAAGCGCGCGGTGCGCGACGTCAACCCGGCGCTGCGCG
ATTTCGAGGCATCGTGCTTCGACGGCCACTACGTGACCGGCGATATCGACGAAGCGTATCTCGACCGGCTGGAAGCGTCG
CGCAACAGCGCGGCGGCCTGCGCGGCCCGCGACAGCGCGCAGAACGACAATCCGCGCACGCAGCTGCACTTGCAGCCGTC
GGCCGTCAACGAGTAA

Upstream 100 bases:

>100_bases
TATTGAGGGGGAGCGTCTGGCCACGAGCCGGGCGGTCCATGCTTTTTCGGTGCCCGCAACGGGTGTGGGCGCCACCAATA
CGTTCTCGCTGGAGATCGAC

Downstream 100 bases:

>100_bases
GGGGCGGGCGGTTCGCGGACGGTGGCGCACCCGGCCCATGGGCTGTGCGCTGCTGTTTTCGTGTACTGGTCTCCAGCAAA
AAGCCCGTCCTGGTGACGGG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MCGIVGVVSATPVNQLIYDSLLLLQHRGQDAAGIATAAGSTFHMHKANGMVRDVFRTRNMRGLPGTIGIGQVRYPTAGSA
SEEEAQPFYVSAPFGIVLAHNGNLTNSEQLREEMFRRDRRHINTHSDSEVLLNVLADELQRASNDIALSPEAIFKAVAGM
HRRVRGSYAIVSQIAGYGMLAVRDPFGIRPLALGSQETPDGVEWMVASESVALEGIGFKFERDVAPGEAIFVDLEGQLHT
KQCADHPVLTPCIFEYVYLARPDSRMDGASVYDARLRMGDYLAEKIKREVTDPIDVVMPIPDSSRPAAMQVANCLGVPYR
EGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMAIEFKGKNVLIVDDSIVRGTTSSEIVQMARDAGAKKVIFASAAPPVKF
PNVYGIDMPTRSELVAHGRTHDEIARIIGADQLVYQDVEDMKRAVRDVNPALRDFEASCFDGHYVTGDIDEAYLDRLEAS
RNSAAACAARDSAQNDNPRTQLHLQPSAVNE

Sequences:

>Translated_511_residues
MCGIVGVVSATPVNQLIYDSLLLLQHRGQDAAGIATAAGSTFHMHKANGMVRDVFRTRNMRGLPGTIGIGQVRYPTAGSA
SEEEAQPFYVSAPFGIVLAHNGNLTNSEQLREEMFRRDRRHINTHSDSEVLLNVLADELQRASNDIALSPEAIFKAVAGM
HRRVRGSYAIVSQIAGYGMLAVRDPFGIRPLALGSQETPDGVEWMVASESVALEGIGFKFERDVAPGEAIFVDLEGQLHT
KQCADHPVLTPCIFEYVYLARPDSRMDGASVYDARLRMGDYLAEKIKREVTDPIDVVMPIPDSSRPAAMQVANCLGVPYR
EGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMAIEFKGKNVLIVDDSIVRGTTSSEIVQMARDAGAKKVIFASAAPPVKF
PNVYGIDMPTRSELVAHGRTHDEIARIIGADQLVYQDVEDMKRAVRDVNPALRDFEASCFDGHYVTGDIDEAYLDRLEAS
RNSAAACAARDSAQNDNPRTQLHLQPSAVNE
>Mature_511_residues
MCGIVGVVSATPVNQLIYDSLLLLQHRGQDAAGIATAAGSTFHMHKANGMVRDVFRTRNMRGLPGTIGIGQVRYPTAGSA
SEEEAQPFYVSAPFGIVLAHNGNLTNSEQLREEMFRRDRRHINTHSDSEVLLNVLADELQRASNDIALSPEAIFKAVAGM
HRRVRGSYAIVSQIAGYGMLAVRDPFGIRPLALGSQETPDGVEWMVASESVALEGIGFKFERDVAPGEAIFVDLEGQLHT
KQCADHPVLTPCIFEYVYLARPDSRMDGASVYDARLRMGDYLAEKIKREVTDPIDVVMPIPDSSRPAAMQVANCLGVPYR
EGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMAIEFKGKNVLIVDDSIVRGTTSSEIVQMARDAGAKKVIFASAAPPVKF
PNVYGIDMPTRSELVAHGRTHDEIARIIGADQLVYQDVEDMKRAVRDVNPALRDFEASCFDGHYVTGDIDEAYLDRLEAS
RNSAAACAARDSAQNDNPRTQLHLQPSAVNE

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=488, Percent_Identity=37.2950819672131, Blast_Score=288, Evalue=1e-77,
Organism=Escherichia coli, GI1788651, Length=506, Percent_Identity=59.6837944664032, Blast_Score=610, Evalue=1e-176,
Organism=Escherichia coli, GI1790167, Length=173, Percent_Identity=32.3699421965318, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17554892, Length=488, Percent_Identity=34.2213114754098, Blast_Score=241, Evalue=5e-64,
Organism=Saccharomyces cerevisiae, GI6323958, Length=505, Percent_Identity=49.7029702970297, Blast_Score=481, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24659598, Length=499, Percent_Identity=37.4749498997996, Blast_Score=289, Evalue=3e-78,
Organism=Drosophila melanogaster, GI24659604, Length=479, Percent_Identity=38.4133611691023, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI28573187, Length=504, Percent_Identity=36.5079365079365, Blast_Score=278, Evalue=6e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 56064; Mature: 56064

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGVVSATPVNQLIYDSLLLLQHRGQDAAGIATAAGSTFHMHKANGMVRDVFRTRNM
CCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEECCCCHHHHHHHHCCC
RGLPGTIGIGQVRYPTAGSASEEEAQPFYVSAPFGIVLAHNGNLTNSEQLREEMFRRDRR
CCCCCCCCCCCEECCCCCCCCCHHCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHH
HINTHSDSEVLLNVLADELQRASNDIALSPEAIFKAVAGMHRRVRGSYAIVSQIAGYGML
HCCCCCHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEE
AVRDPFGIRPLALGSQETPDGVEWMVASESVALEGIGFKFERDVAPGEAIFVDLEGQLHT
EEECCCCCCEEECCCCCCCCCCEEEEECCCEEEECCCEEEECCCCCCCEEEEEECCCCCH
KQCADHPVLTPCIFEYVYLARPDSRMDGASVYDARLRMGDYLAEKIKREVTDPIDVVMPI
HHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
PDSSRPAAMQVANCLGVPYREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMAIEFKGKN
CCCCCCHHHHHHHHHCCCHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHEEEEECCCE
VLIVDDSIVRGTTSSEIVQMARDAGAKKVIFASAAPPVKFPNVYGIDMPTRSELVAHGRT
EEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHCCCC
HDEIARIIGADQLVYQDVEDMKRAVRDVNPALRDFEASCFDGHYVTGDIDEAYLDRLEAS
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHCCCHHCCCCEEECCCCHHHHHHHHHC
RNSAAACAARDSAQNDNPRTQLHLQPSAVNE
CCCHHHHHHCCCCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MCGIVGVVSATPVNQLIYDSLLLLQHRGQDAAGIATAAGSTFHMHKANGMVRDVFRTRNM
CCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEECCCCHHHHHHHHCCC
RGLPGTIGIGQVRYPTAGSASEEEAQPFYVSAPFGIVLAHNGNLTNSEQLREEMFRRDRR
CCCCCCCCCCCEECCCCCCCCCHHCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHH
HINTHSDSEVLLNVLADELQRASNDIALSPEAIFKAVAGMHRRVRGSYAIVSQIAGYGML
HCCCCCHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEE
AVRDPFGIRPLALGSQETPDGVEWMVASESVALEGIGFKFERDVAPGEAIFVDLEGQLHT
EEECCCCCCEEECCCCCCCCCCEEEEECCCEEEECCCEEEECCCCCCCEEEEEECCCCCH
KQCADHPVLTPCIFEYVYLARPDSRMDGASVYDARLRMGDYLAEKIKREVTDPIDVVMPI
HHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
PDSSRPAAMQVANCLGVPYREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMAIEFKGKN
CCCCCCHHHHHHHHHCCCHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHEEEEECCCE
VLIVDDSIVRGTTSSEIVQMARDAGAKKVIFASAAPPVKFPNVYGIDMPTRSELVAHGRT
EEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHCCCC
HDEIARIIGADQLVYQDVEDMKRAVRDVNPALRDFEASCFDGHYVTGDIDEAYLDRLEAS
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHCCCHHCCCCEEECCCCHHHHHHHHHC
RNSAAACAARDSAQNDNPRTQLHLQPSAVNE
CCCHHHHHHCCCCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]