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The map label for this gene is tISRso10b [C]

Identifier: 17546549

GI number: 17546549

Start: 2001545

End: 2002393

Strand: Reverse

Name: tISRso10b [C]

Synonym: RSc1830

Alternate gene names: 17546549

Gene position: 2002393-2001545 (Counterclockwise)

Preceding gene: 17546550

Following gene: 17546548

Centisome position: 53.88

GC content: 64.08

Gene sequence:

>849_bases
GTGAAGCTAGTTTGCGAGGTCCTCGGCGTAGCGCGCTCAAACGTGGCGGCCAAGCGGGCCCGGCCGGGTGATTGGCGCGA
CGGGCGCACAGCTCGGCAAACCGACGACGCTAGCTTGGTCGACGAAATCCGGCGCACCATCGCCGACTTGCCGAGCTATG
GCTACCGGCGGGTCTGGGGCAGATTGCGGCGCGAACGTGAGCGTCAATGCGAAGCGCCGATCAATGCCAAGCGTGTCTAT
CGGGTCATGCGTGTGCACAGCTTGCTGCTTGAGCGCAGGCCGACGCCACCGCGTCCACCGCGCCGGCATGACGGCAAGGT
CGCCGTGGCCAAGAGCAACCAGCGCTGGTGCTCCGATGGCTTCGAGTTCCGCTGCGACAACGGCGAGCCGCTGCGGGTGA
CGTTCGCGCTGGATTGCTGCGACCGCGAGGCGATGAGCTGGGTGGCGACGACCGGCGGCTACAGCGGCGACGTAGTACGC
GACGTGATGCTGGCGGCCGTCGAGCAGCGCTTCGGCAATGTTCCGAAAGCGCCAGCGGAAATTGAATGGCTGACGGACAA
CGGCTCAGGCTACATTGCTGGGAAGACTCGCGAGTTCGCCACGGACATTGGCTTGAAGCCATTGACGACACCGGTTTGCA
GCCCGCAGAGCAACGGCATGGCCGAGAGCTTCGTGAAGACGATGAAGCGCGACTACGTCGCCTTCATGCCCAAGCCTGAC
GCGGCCACAGCGACGCGCAACCTCGCCATCGCGTTCGAGCATTACAACGAGCAGCATCCCCATAGCGCACTGAAATATCG
CTCGCCACGCGAGTTCAGGCGCAGGACGGAATCATCAACTCAAGTGTGA

Upstream 100 bases:

>100_bases
GCAAGAAGACGATGGAGAACGAGATTCTTCGCGAAGCCGTGGAATACGGTCGGGCAAAAAAGTGGATAGCGCGCTCGCCA
TCGTTGCCGGGGGACGACCA

Downstream 100 bases:

>100_bases
GGCGGTGTCCGGAGATGCGGGGGCAAATCCAGCGCGGGCGAGGTTTTCAGCGGCGAGGGCATCCTGGCCGTGACCAAGGC
GCTGCTGGAATCGGGCGTCG

Product: ISRSO10-transposase ORFB protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MKLVCEVLGVARSNVAAKRARPGDWRDGRTARQTDDASLVDEIRRTIADLPSYGYRRVWGRLRRERERQCEAPINAKRVY
RVMRVHSLLLERRPTPPRPPRRHDGKVAVAKSNQRWCSDGFEFRCDNGEPLRVTFALDCCDREAMSWVATTGGYSGDVVR
DVMLAAVEQRFGNVPKAPAEIEWLTDNGSGYIAGKTREFATDIGLKPLTTPVCSPQSNGMAESFVKTMKRDYVAFMPKPD
AATATRNLAIAFEHYNEQHPHSALKYRSPREFRRRTESSTQV

Sequences:

>Translated_282_residues
MKLVCEVLGVARSNVAAKRARPGDWRDGRTARQTDDASLVDEIRRTIADLPSYGYRRVWGRLRRERERQCEAPINAKRVY
RVMRVHSLLLERRPTPPRPPRRHDGKVAVAKSNQRWCSDGFEFRCDNGEPLRVTFALDCCDREAMSWVATTGGYSGDVVR
DVMLAAVEQRFGNVPKAPAEIEWLTDNGSGYIAGKTREFATDIGLKPLTTPVCSPQSNGMAESFVKTMKRDYVAFMPKPD
AATATRNLAIAFEHYNEQHPHSALKYRSPREFRRRTESSTQV
>Mature_282_residues
MKLVCEVLGVARSNVAAKRARPGDWRDGRTARQTDDASLVDEIRRTIADLPSYGYRRVWGRLRRERERQCEAPINAKRVY
RVMRVHSLLLERRPTPPRPPRRHDGKVAVAKSNQRWCSDGFEFRCDNGEPLRVTFALDCCDREAMSWVATTGGYSGDVVR
DVMLAAVEQRFGNVPKAPAEIEWLTDNGSGYIAGKTREFATDIGLKPLTTPVCSPQSNGMAESFVKTMKRDYVAFMPKPD
AATATRNLAIAFEHYNEQHPHSALKYRSPREFRRRTESSTQV

Specific function: Involved in the transposition of the insertion sequence is2 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1790724, Length=276, Percent_Identity=60.8695652173913, Blast_Score=343, Evalue=5e-96,
Organism=Escherichia coli, GI1789424, Length=276, Percent_Identity=60.8695652173913, Blast_Score=343, Evalue=5e-96,
Organism=Escherichia coli, GI1789224, Length=276, Percent_Identity=60.8695652173913, Blast_Score=343, Evalue=5e-96,
Organism=Escherichia coli, GI1788305, Length=276, Percent_Identity=60.8695652173913, Blast_Score=343, Evalue=5e-96,
Organism=Escherichia coli, GI1787669, Length=276, Percent_Identity=60.8695652173913, Blast_Score=343, Evalue=5e-96,
Organism=Escherichia coli, GI1786558, Length=276, Percent_Identity=60.8695652173913, Blast_Score=343, Evalue=5e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR002514 [H]

Pfam domain/function: PF00665 rve; PF01527 Transposase_8 [H]

EC number: NA

Molecular weight: Translated: 32031; Mature: 32031

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVCEVLGVARSNVAAKRARPGDWRDGRTARQTDDASLVDEIRRTIADLPSYGYRRVWG
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
RLRRERERQCEAPINAKRVYRVMRVHSLLLERRPTPPRPPRRHDGKVAVAKSNQRWCSDG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHCCC
FEFRCDNGEPLRVTFALDCCDREAMSWVATTGGYSGDVVRDVMLAAVEQRFGNVPKAPAE
CEEEECCCCEEEEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCE
IEWLTDNGSGYIAGKTREFATDIGLKPLTTPVCSPQSNGMAESFVKTMKRDYVAFMPKPD
EEEEEECCCEEEECCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCC
AATATRNLAIAFEHYNEQHPHSALKYRSPREFRRRTESSTQV
CHHHHHHEEEEEECCCCCCCHHHHHCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MKLVCEVLGVARSNVAAKRARPGDWRDGRTARQTDDASLVDEIRRTIADLPSYGYRRVWG
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
RLRRERERQCEAPINAKRVYRVMRVHSLLLERRPTPPRPPRRHDGKVAVAKSNQRWCSDG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHCCC
FEFRCDNGEPLRVTFALDCCDREAMSWVATTGGYSGDVVRDVMLAAVEQRFGNVPKAPAE
CEEEECCCCEEEEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCE
IEWLTDNGSGYIAGKTREFATDIGLKPLTTPVCSPQSNGMAESFVKTMKRDYVAFMPKPD
EEEEEECCCEEEECCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCC
AATATRNLAIAFEHYNEQHPHSALKYRSPREFRRRTESSTQV
CHHHHHHEEEEEECCCCCCCHHHHHCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA