BacMap

Definition	Ralstonia solanacearum GMI1000, complete genome.
Accession	NC_003295
Length	3,716,413

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The map label for this gene is cbiO2 [H]

Identifier: 17546457

GI number: 17546457

Start: 1860386

End: 1861180

Strand: Reverse

Name: cbiO2 [H]

Synonym: RSc1738

Alternate gene names: 17546457

Gene position: 1861180-1860386 (Counterclockwise)

Preceding gene: 17546458

Following gene: 17546455

Centisome position: 50.08

GC content: 68.81

Gene sequence:

>795_bases
ATGCTGAGCGCACAAGGACTCACCCTCACCTTCAACGCCGGCACGCCCATCGAGACGCGCGCGCTGCGCGGCCTGTCGCT
GGAGATCCCGACCGGGCAGTTCGTCACCGTGATCGGCTCCAACGGCGCCGGCAAATCGACCTTCCTGAACTCCATCAGCG
GCGATCAGATGGTCGACGCGGGCCGCATCACCATCGACGGCATCGACGTCACGCGCAAGACGGCGTGGCAGCGCGCCAAC
CTGGTGGCGCGCGTGTTCCAGGACCCGATGGCCGGCACCTGCGAGAACCTGACCATCGAAGAGAACATGTCGCTGGCCAT
GTGCCGCGGGCAGCGGCGCGGGTTCGGCTTCGCCACCAACCGCAAGTGGCGCGAGGTTTTCCGCGCGCAGCTGCGCCGGC
TCGACCTCGGCCTGGAGAACCGCCTGACCGACCGCATCGGCCTGCTCTCGGGCGGCCAGCGCCAGGCGGTGAGCCTGCTG
ATGGCCTCGCTGCAGCCGTCGCGCATCCTGCTGCTGGACGAGCACACCGCCGCGCTCGACCCGAAGACGGCCGCCTTCGT
GCTGGAACTGACCGCCAAGATCGTCTCGGAGAGCAAGCTGACGACGATGATGGTCACGCACTCGATGCGCCAGGCGCTCG
ACTACGGCCAGCGCACGGTGATGCTGCACCAGGGCAACGTGATCCTCGACGTGCAGGGCGGCGAGCGCGCCGGCCTGGAC
GTGCCGCACCTGCTGCAGAAGTTCGAGGAAACGCGCGGCGCCAAGGTCGACGACGACGCGCTGCTGCTGGGCTGA

Upstream 100 bases:

>100_bases
GTGACCGCCGCGCTGGTGGTGATCGCCCTGGTGCTGCCCGGCATGCGCCGCAAGGTGTTCGGCGCGCTCTCCCGCGGCAA
CGGCAACAAGGGGAACTGAC

Downstream 100 bases:

>100_bases
TCCGGCACGCACCATGAAAAACGGGCACCTTCGGGTGCCCGTTGTCGTTGTGCCGGGCTACCCCGGCAGCGGTGCGATCA
GAAGCGGTGCTGCAGGCCCG

Product: ABC transporter ATP-binding protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MLSAQGLTLTFNAGTPIETRALRGLSLEIPTGQFVTVIGSNGAGKSTFLNSISGDQMVDAGRITIDGIDVTRKTAWQRAN
LVARVFQDPMAGTCENLTIEENMSLAMCRGQRRGFGFATNRKWREVFRAQLRRLDLGLENRLTDRIGLLSGGQRQAVSLL
MASLQPSRILLLDEHTAALDPKTAAFVLELTAKIVSESKLTTMMVTHSMRQALDYGQRTVMLHQGNVILDVQGGERAGLD
VPHLLQKFEETRGAKVDDDALLLG

Sequences:

>Translated_264_residues
MLSAQGLTLTFNAGTPIETRALRGLSLEIPTGQFVTVIGSNGAGKSTFLNSISGDQMVDAGRITIDGIDVTRKTAWQRAN
LVARVFQDPMAGTCENLTIEENMSLAMCRGQRRGFGFATNRKWREVFRAQLRRLDLGLENRLTDRIGLLSGGQRQAVSLL
MASLQPSRILLLDEHTAALDPKTAAFVLELTAKIVSESKLTTMMVTHSMRQALDYGQRTVMLHQGNVILDVQGGERAGLD
VPHLLQKFEETRGAKVDDDALLLG
>Mature_264_residues
MLSAQGLTLTFNAGTPIETRALRGLSLEIPTGQFVTVIGSNGAGKSTFLNSISGDQMVDAGRITIDGIDVTRKTAWQRAN
LVARVFQDPMAGTCENLTIEENMSLAMCRGQRRGFGFATNRKWREVFRAQLRRLDLGLENRLTDRIGLLSGGQRQAVSLL
MASLQPSRILLLDEHTAALDPKTAAFVLELTAKIVSESKLTTMMVTHSMRQALDYGQRTVMLHQGNVILDVQGGERAGLD
VPHLLQKFEETRGAKVDDDALLLG

Specific function: Part of the ABC transporter complex CbiONQ involved in cobalt import. Responsible for energy coupling to the transport system [H]

COG id: COG1101

COG function: function code R; ABC-type uncharacterized transport system, ATPase component

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI9961250, Length=212, Percent_Identity=30.6603773584906, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI42741659, Length=189, Percent_Identity=30.6878306878307, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI116734710, Length=214, Percent_Identity=28.0373831775701, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI9961252, Length=205, Percent_Identity=30.2439024390244, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI4505771, Length=205, Percent_Identity=30.2439024390244, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI27881501, Length=210, Percent_Identity=25.7142857142857, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI30795238, Length=210, Percent_Identity=25.7142857142857, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI47078218, Length=216, Percent_Identity=28.2407407407407, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI45446740, Length=216, Percent_Identity=28.2407407407407, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI4557481, Length=216, Percent_Identity=28.7037037037037, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1786253, Length=205, Percent_Identity=32.6829268292683, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1788761, Length=211, Percent_Identity=32.7014218009479, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI87082268, Length=222, Percent_Identity=31.5315315315315, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790544, Length=242, Percent_Identity=28.5123966942149, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87081709, Length=227, Percent_Identity=29.0748898678414, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1788225, Length=245, Percent_Identity=31.0204081632653, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787712, Length=213, Percent_Identity=31.4553990610329, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787089, Length=225, Percent_Identity=31.5555555555556, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786803, Length=259, Percent_Identity=27.7992277992278, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787370, Length=206, Percent_Identity=32.0388349514563, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787164, Length=241, Percent_Identity=29.4605809128631, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1786872, Length=195, Percent_Identity=31.7948717948718, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1786345, Length=209, Percent_Identity=33.9712918660287, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082267, Length=225, Percent_Identity=29.7777777777778, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1786398, Length=213, Percent_Identity=30.5164319248826, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081791, Length=231, Percent_Identity=25.974025974026, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787029, Length=217, Percent_Identity=29.0322580645161, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787143, Length=164, Percent_Identity=34.7560975609756, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87081834, Length=216, Percent_Identity=24.537037037037, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787105, Length=215, Percent_Identity=27.906976744186, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1789864, Length=237, Percent_Identity=28.6919831223629, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1790739, Length=220, Percent_Identity=28.1818181818182, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788506, Length=253, Percent_Identity=26.8774703557312, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789032, Length=215, Percent_Identity=27.906976744186, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789963, Length=242, Percent_Identity=25.6198347107438, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1787792, Length=221, Percent_Identity=25.3393665158371, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787500, Length=219, Percent_Identity=25.1141552511416, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786319, Length=213, Percent_Identity=26.7605633802817, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790190, Length=205, Percent_Identity=24.8780487804878, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790162, Length=223, Percent_Identity=28.6995515695067, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI48994997, Length=234, Percent_Identity=24.7863247863248, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787113, Length=234, Percent_Identity=31.1965811965812, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786563, Length=211, Percent_Identity=28.9099526066351, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI48995001, Length=245, Percent_Identity=22.8571428571429, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789891, Length=220, Percent_Identity=26.8181818181818, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787112, Length=212, Percent_Identity=27.8301886792453, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787548, Length=249, Percent_Identity=22.8915662650602, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788450, Length=216, Percent_Identity=27.7777777777778, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786703, Length=200, Percent_Identity=30.5, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1790525, Length=212, Percent_Identity=22.6415094339623, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1786654, Length=211, Percent_Identity=26.0663507109005, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1787758, Length=213, Percent_Identity=23.943661971831, Blast_Score=61, Evalue=8e-11,
Organism=Escherichia coli, GI87081782, Length=206, Percent_Identity=27.1844660194175, Blast_Score=61, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17541710, Length=218, Percent_Identity=34.8623853211009, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71983510, Length=211, Percent_Identity=30.3317535545024, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI71983523, Length=211, Percent_Identity=30.3317535545024, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI71983516, Length=211, Percent_Identity=30.3317535545024, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI212646699, Length=224, Percent_Identity=27.2321428571429, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71996809, Length=229, Percent_Identity=26.2008733624454, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI193211015, Length=229, Percent_Identity=26.2008733624454, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI193202349, Length=250, Percent_Identity=27.6, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI115533592, Length=187, Percent_Identity=31.0160427807487, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI115534520, Length=206, Percent_Identity=30.5825242718447, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI32566680, Length=245, Percent_Identity=28.1632653061224, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17510237, Length=211, Percent_Identity=29.3838862559242, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI193211017, Length=229, Percent_Identity=26.2008733624454, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71984940, Length=210, Percent_Identity=30.952380952381, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17543740, Length=207, Percent_Identity=27.0531400966184, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193208177, Length=216, Percent_Identity=25.462962962963, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17558664, Length=178, Percent_Identity=31.4606741573034, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17565938, Length=206, Percent_Identity=25.7281553398058, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI193209708, Length=214, Percent_Identity=26.1682242990654, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25146777, Length=193, Percent_Identity=30.0518134715026, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17569081, Length=210, Percent_Identity=27.1428571428571, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI71988343, Length=203, Percent_Identity=30.0492610837438, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71992285, Length=235, Percent_Identity=27.2340425531915, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115535101, Length=223, Percent_Identity=28.6995515695067, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17567269, Length=221, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17569143, Length=207, Percent_Identity=30.4347826086957, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17567265, Length=248, Percent_Identity=28.6290322580645, Blast_Score=64, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17551700, Length=221, Percent_Identity=28.5067873303167, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6323959, Length=220, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320339, Length=213, Percent_Identity=28.6384976525822, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24649703, Length=235, Percent_Identity=29.7872340425532, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24659289, Length=213, Percent_Identity=30.0469483568075, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281363262, Length=232, Percent_Identity=28.8793103448276, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI85726357, Length=209, Percent_Identity=29.6650717703349, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24653245, Length=232, Percent_Identity=28.8793103448276, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI17136662, Length=210, Percent_Identity=30, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI221474134, Length=214, Percent_Identity=31.3084112149533, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21357907, Length=218, Percent_Identity=27.0642201834862, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR015856
- InterPro:   IPR003593
- InterPro:   IPR022112 [H]

Pfam domain/function: PF00005 ABC_tran; PF12370 CoABC_C [H]

EC number: NA

Molecular weight: Translated: 28866; Mature: 28866

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSAQGLTLTFNAGTPIETRALRGLSLEIPTGQFVTVIGSNGAGKSTFLNSISGDQMVDA
CCCCCCEEEEECCCCCCHHHHHCCEEEECCCCCEEEEEECCCCCHHHHHHCCCCCCEECC
GRITIDGIDVTRKTAWQRANLVARVFQDPMAGTCENLTIEENMSLAMCRGQRRGFGFATN
CEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCEEEECCCCCCCCCCCCC
RKWREVFRAQLRRLDLGLENRLTDRIGLLSGGQRQAVSLLMASLQPSRILLLDEHTAALD
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCC
PKTAAFVLELTAKIVSESKLTTMMVTHSMRQALDYGQRTVMLHQGNVILDVQGGERAGLD
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCCCCCC
VPHLLQKFEETRGAKVDDDALLLG
HHHHHHHHHHHCCCCCCCCCEEEC
>Mature Secondary Structure
MLSAQGLTLTFNAGTPIETRALRGLSLEIPTGQFVTVIGSNGAGKSTFLNSISGDQMVDA
CCCCCCEEEEECCCCCCHHHHHCCEEEECCCCCEEEEEECCCCCHHHHHHCCCCCCEECC
GRITIDGIDVTRKTAWQRANLVARVFQDPMAGTCENLTIEENMSLAMCRGQRRGFGFATN
CEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCEEEECCCCCCCCCCCCC
RKWREVFRAQLRRLDLGLENRLTDRIGLLSGGQRQAVSLLMASLQPSRILLLDEHTAALD
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCC
PKTAAFVLELTAKIVSESKLTTMMVTHSMRQALDYGQRTVMLHQGNVILDVQGGERAGLD
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCCCCCC
VPHLLQKFEETRGAKVDDDALLLG
HHHHHHHHHHHCCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA