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The map label for this gene is potD [H]

Identifier: 17546452

GI number: 17546452

Start: 1854256

End: 1855296

Strand: Reverse

Name: potD [H]

Synonym: RSc1733

Alternate gene names: 17546452

Gene position: 1855296-1854256 (Counterclockwise)

Preceding gene: 17546453

Following gene: 17546451

Centisome position: 49.92

GC content: 66.09

Gene sequence:

>1041_bases
ATGAAACCCATCGCGAAGACGCGCCTGAGCTTGCTCGCCGCAGCATTCGCCGTCGCCTGCGGCGCGCACGCGGCGGAGGT
CACCGTCGTCAACTTCGGCGGCGCCAACGGCGACGCGCAGAAGGCCGCATTCAACAAGCCGTTCGAGGCGCAGACCGGCA
ACAAGGTCACCGCCGTCGAGTACAACGGCGAGCAGGCCAAGATCAAGGCCATGGTCGAAGCCAAGCACGTCAACTGGGAC
GTGGTCGAAGTCGAGTCGGGCGACATCGGCCGCGGCTGCGACGAGGGCCTGTTCGAGAAGCTCGACTGGTCCAAGATCGC
CAAGAAGGCCGACCTGGTGCCCGAGGCGCCGCACACCTGCGGCGTCGGCTTCTTCGTCTGGTCGACGGCGCTGGCCTACA
ACGCCGACAAGCTCAAGGCCGCCCCCAGCGGCTGGGCCGACTTCTGGGACGTGAAGAAATTCCCGGGCAAGCGCGGCATG
CGCAAGGGCGCGCGCTACAACCTGGAGTTCGCGCTGATGGCCGACGGCGTGCCGGTCAAGGACGTCTACAAAGTGCTCGC
CACCAAGGACGGCCAGAACCGCGCCTTCAAGAAGCTCGACCAGCTCAAGCCCTACATCCAGTGGTGGGAAGCCGGCGCGC
AGCCGCCGCAGTTCCTGGTGGCCGGCGACGTGGTGATGTCCACCGCCTTCAACGGCCGCATCGACGCCGCGCAGCGCGAA
GGCAAGAACCTCAAGGTGGTCTGGAACGGCAGCATCTACGACCTGGACTACTGGGCGATCCCCAAGGGCACGCCGAACAA
GGCGCTGGCCGAGCAGTACATCGCCTACACGCTGTCGCAGAAGGCCCAGCAGGAATACGCCAAGCACATCGCCTACGGCC
CGGCCAACCTGGCCGCGATCAAGGCGCTCGATGCGAAGACCCAGGCCAACCTGCCCAACTCGCCCGAGAACGGCAAGAGC
GCGGTGCTGCAGAACCTGCAGTTCTGGACCGACCACGGCGATGAGCTGGAGCAGCGCTTCGCCTCGTGGGCATCGAAGTA
A

Upstream 100 bases:

>100_bases
GCGCGTCTTCACCTGAATCACCGGGCGCGCGGGGCCCCGGCCCCGGGGCCCATCGACCACAGCAGCAACCACACGCCAAA
GCCAAAAGAGAGGAGCCAAC

Downstream 100 bases:

>100_bases
GCAGGCCGCCCGGGCCGGCGCGCTCGCATGCTCCGGCATGCCCCGGCGCCGGCCCGACATGAAGGGTGCAGGACACGGCA
GGTGCAGAATTGAACACCAT

Product: lipoprotein signal peptide

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MKPIAKTRLSLLAAAFAVACGAHAAEVTVVNFGGANGDAQKAAFNKPFEAQTGNKVTAVEYNGEQAKIKAMVEAKHVNWD
VVEVESGDIGRGCDEGLFEKLDWSKIAKKADLVPEAPHTCGVGFFVWSTALAYNADKLKAAPSGWADFWDVKKFPGKRGM
RKGARYNLEFALMADGVPVKDVYKVLATKDGQNRAFKKLDQLKPYIQWWEAGAQPPQFLVAGDVVMSTAFNGRIDAAQRE
GKNLKVVWNGSIYDLDYWAIPKGTPNKALAEQYIAYTLSQKAQQEYAKHIAYGPANLAAIKALDAKTQANLPNSPENGKS
AVLQNLQFWTDHGDELEQRFASWASK

Sequences:

>Translated_346_residues
MKPIAKTRLSLLAAAFAVACGAHAAEVTVVNFGGANGDAQKAAFNKPFEAQTGNKVTAVEYNGEQAKIKAMVEAKHVNWD
VVEVESGDIGRGCDEGLFEKLDWSKIAKKADLVPEAPHTCGVGFFVWSTALAYNADKLKAAPSGWADFWDVKKFPGKRGM
RKGARYNLEFALMADGVPVKDVYKVLATKDGQNRAFKKLDQLKPYIQWWEAGAQPPQFLVAGDVVMSTAFNGRIDAAQRE
GKNLKVVWNGSIYDLDYWAIPKGTPNKALAEQYIAYTLSQKAQQEYAKHIAYGPANLAAIKALDAKTQANLPNSPENGKS
AVLQNLQFWTDHGDELEQRFASWASK
>Mature_346_residues
MKPIAKTRLSLLAAAFAVACGAHAAEVTVVNFGGANGDAQKAAFNKPFEAQTGNKVTAVEYNGEQAKIKAMVEAKHVNWD
VVEVESGDIGRGCDEGLFEKLDWSKIAKKADLVPEAPHTCGVGFFVWSTALAYNADKLKAAPSGWADFWDVKKFPGKRGM
RKGARYNLEFALMADGVPVKDVYKVLATKDGQNRAFKKLDQLKPYIQWWEAGAQPPQFLVAGDVVMSTAFNGRIDAAQRE
GKNLKVVWNGSIYDLDYWAIPKGTPNKALAEQYIAYTLSQKAQQEYAKHIAYGPANLAAIKALDAKTQANLPNSPENGKS
AVLQNLQFWTDHGDELEQRFASWASK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=268, Percent_Identity=25.7462686567164, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37874; Mature: 37874

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIAKTRLSLLAAAFAVACGAHAAEVTVVNFGGANGDAQKAAFNKPFEAQTGNKVTAVE
CCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHCCCCCCCCCCCEEEEEE
YNGEQAKIKAMVEAKHVNWDVVEVESGDIGRGCDEGLFEKLDWSKIAKKADLVPEAPHTC
ECCCCEEEEEEEEHCCCCEEEEEECCCCCCCCCCHHHHHHCCHHHHHHHHCCCCCCCCCC
GVGFFVWSTALAYNADKLKAAPSGWADFWDVKKFPGKRGMRKGARYNLEFALMADGVPVK
CHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHCCCCCCHHCCCCCCEEEEEEECCCCHH
DVYKVLATKDGQNRAFKKLDQLKPYIQWWEAGAQPPQFLVAGDVVMSTAFNGRIDAAQRE
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHCCCCCCCHHHHC
GKNLKVVWNGSIYDLDYWAIPKGTPNKALAEQYIAYTLSQKAQQEYAKHIAYGPANLAAI
CCEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
KALDAKTQANLPNSPENGKSAVLQNLQFWTDHGDELEQRFASWASK
HHHCCHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKPIAKTRLSLLAAAFAVACGAHAAEVTVVNFGGANGDAQKAAFNKPFEAQTGNKVTAVE
CCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHCCCCCCCCCCCEEEEEE
YNGEQAKIKAMVEAKHVNWDVVEVESGDIGRGCDEGLFEKLDWSKIAKKADLVPEAPHTC
ECCCCEEEEEEEEHCCCCEEEEEECCCCCCCCCCHHHHHHCCHHHHHHHHCCCCCCCCCC
GVGFFVWSTALAYNADKLKAAPSGWADFWDVKKFPGKRGMRKGARYNLEFALMADGVPVK
CHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHCCCCCCHHCCCCCCEEEEEEECCCCHH
DVYKVLATKDGQNRAFKKLDQLKPYIQWWEAGAQPPQFLVAGDVVMSTAFNGRIDAAQRE
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHCCCCCCCHHHHC
GKNLKVVWNGSIYDLDYWAIPKGTPNKALAEQYIAYTLSQKAQQEYAKHIAYGPANLAAI
CCEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
KALDAKTQANLPNSPENGKSAVLQNLQFWTDHGDELEQRFASWASK
HHHCCHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]