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The map label for this gene is tISRso18 [H]

Identifier: 17546423

GI number: 17546423

Start: 1820273

End: 1821238

Strand: Reverse

Name: tISRso18 [H]

Synonym: RSc1704

Alternate gene names: 17546423

Gene position: 1821238-1820273 (Counterclockwise)

Preceding gene: 17546424

Following gene: 17546416

Centisome position: 49.01

GC content: 62.01

Gene sequence:

>966_bases
ATGAAACAAACCGACCTTGGCCTGGACCTGAGCAACCGGCGCACGCGCAAGCAGGTATTTCTCGACGAGATGGAGCGCGT
GGTGCCATGGCAGGCGTTTTTGGCGCTGATAGCACCGCATGCGCCGACCAAGGCGACCGGCCGCAAGCCGTTTCCAATCG
AGGCGATGCTGCGCATCCACTTGTTGCAGCAATGGTTCGGGCTGTCGGACGTGGCGATGGAAGAGGCGCTGTACGACGTG
CCGTTGTATCGTCAATTCGCGGGTCTGGGTGGCATGAGCCGGCTGCCGGACCGGGTCAGCATTCTGCGCTTCCGGCACTT
GCTGGAGCGGCACAAGCTGGCCGAGCAGTTTCTGCAGACCGTCAACGAGCAACTCAGTGCCAAGGGGTACCTGCTCAAAG
AGGGCACGGCGGTCGATGCCACGCTGATTTCCGCGCCCAGCTCGACGAAGAACCAAAGCGGCGAGCGCGACCCTGAGATG
CACCAGACCAAGAAAGGCAACCAGTGGCATTTCGGCATGAAGGCGCACATCGGTGTGGATGCAGACTCGGGGCTGGTGCA
TACCGTGGTGGGCACGGCGGCCAACGTCAACGATGTTACGCAAGCGCATGCTCTGGTGCATGGCAAGGAGGCCGATGTGT
TCGGCGATGCCGGCTACCAGGGCATCGACAAGCGCGAGGAAGTGCAGAAGCTGAAGGTGCGTTGGCATGTGGCATTGCGC
CCGGGCAAGCGCCGCGCCTTGGACAAGAACACCGCGCTGGGCAAGCTCGTTGATGAACTGGAACGGGTCAAGGCGCGCAT
CCGTGCCCGCGCGGAACATCCGTTTCGTGTCATCAAGCGCCAGTTCGGGCATCTGAAGGTTCGCTATCGCGGCTTGATGA
AGAACACGCAACAGTTGCACACGCTGTTCGCCCTGAGCAACCTGTGGATGATGCGCGGGCGACTGATGGGTGAGGCCCGC
GCATGA

Upstream 100 bases:

>100_bases
AAAAATCAAAGCCAGAATTGGTCTTGGCCTCTAAGGATGATGTTCAAAACTCCCGCAAACCCGGACAGTCAAAGGAAGGT
TCGCGAAACGGGATGAGTCC

Downstream 100 bases:

>100_bases
GTGCGCCGGCCATTGGCCAAGCTGCCCAGGAGCCAGAAACCGGCCCGCCTTCAGGCTGAACACGACGCTGTATTCGAGCT
CGCCATCACATCGTTTGCAC

Product: ISRSO18-transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKQTDLGLDLSNRRTRKQVFLDEMERVVPWQAFLALIAPHAPTKATGRKPFPIEAMLRIHLLQQWFGLSDVAMEEALYDV
PLYRQFAGLGGMSRLPDRVSILRFRHLLERHKLAEQFLQTVNEQLSAKGYLLKEGTAVDATLISAPSSTKNQSGERDPEM
HQTKKGNQWHFGMKAHIGVDADSGLVHTVVGTAANVNDVTQAHALVHGKEADVFGDAGYQGIDKREEVQKLKVRWHVALR
PGKRRALDKNTALGKLVDELERVKARIRARAEHPFRVIKRQFGHLKVRYRGLMKNTQQLHTLFALSNLWMMRGRLMGEAR
A

Sequences:

>Translated_321_residues
MKQTDLGLDLSNRRTRKQVFLDEMERVVPWQAFLALIAPHAPTKATGRKPFPIEAMLRIHLLQQWFGLSDVAMEEALYDV
PLYRQFAGLGGMSRLPDRVSILRFRHLLERHKLAEQFLQTVNEQLSAKGYLLKEGTAVDATLISAPSSTKNQSGERDPEM
HQTKKGNQWHFGMKAHIGVDADSGLVHTVVGTAANVNDVTQAHALVHGKEADVFGDAGYQGIDKREEVQKLKVRWHVALR
PGKRRALDKNTALGKLVDELERVKARIRARAEHPFRVIKRQFGHLKVRYRGLMKNTQQLHTLFALSNLWMMRGRLMGEAR
A
>Mature_321_residues
MKQTDLGLDLSNRRTRKQVFLDEMERVVPWQAFLALIAPHAPTKATGRKPFPIEAMLRIHLLQQWFGLSDVAMEEALYDV
PLYRQFAGLGGMSRLPDRVSILRFRHLLERHKLAEQFLQTVNEQLSAKGYLLKEGTAVDATLISAPSSTKNQSGERDPEM
HQTKKGNQWHFGMKAHIGVDADSGLVHTVVGTAANVNDVTQAHALVHGKEADVFGDAGYQGIDKREEVQKLKVRWHVALR
PGKRRALDKNTALGKLVDELERVKARIRARAEHPFRVIKRQFGHLKVRYRGLMKNTQQLHTLFALSNLWMMRGRLMGEAR
A

Specific function: Involved in the transposition of the insertion sequence IS5 [H]

COG id: COG3039

COG function: function code L; Transposase and inactivated derivatives, IS5 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1787634, Length=305, Percent_Identity=55.0819672131148, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1789921, Length=297, Percent_Identity=55.8922558922559, Blast_Score=354, Evalue=5e-99,
Organism=Escherichia coli, GI1789612, Length=297, Percent_Identity=55.8922558922559, Blast_Score=354, Evalue=5e-99,
Organism=Escherichia coli, GI1789355, Length=297, Percent_Identity=55.8922558922559, Blast_Score=354, Evalue=5e-99,
Organism=Escherichia coli, GI1788518, Length=297, Percent_Identity=55.8922558922559, Blast_Score=354, Evalue=5e-99,
Organism=Escherichia coli, GI1788342, Length=297, Percent_Identity=55.8922558922559, Blast_Score=354, Evalue=5e-99,
Organism=Escherichia coli, GI1787592, Length=297, Percent_Identity=55.8922558922559, Blast_Score=354, Evalue=5e-99,
Organism=Escherichia coli, GI1786877, Length=297, Percent_Identity=55.8922558922559, Blast_Score=354, Evalue=5e-99,
Organism=Escherichia coli, GI1786764, Length=297, Percent_Identity=55.8922558922559, Blast_Score=354, Evalue=5e-99,
Organism=Escherichia coli, GI1786453, Length=297, Percent_Identity=55.8922558922559, Blast_Score=354, Evalue=5e-99,
Organism=Escherichia coli, GI1788303, Length=297, Percent_Identity=55.5555555555556, Blast_Score=353, Evalue=1e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002559
- InterPro:   IPR008490 [H]

Pfam domain/function: PF05598 DUF772; PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 36457; Mature: 36457

Theoretical pI: Translated: 10.82; Mature: 10.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTDLGLDLSNRRTRKQVFLDEMERVVPWQAFLALIAPHAPTKATGRKPFPIEAMLRIH
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
LLQQWFGLSDVAMEEALYDVPLYRQFAGLGGMSRLPDRVSILRFRHLLERHKLAEQFLQT
HHHHHHCCHHHHHHHHHHCCHHHHHHHCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHH
VNEQLSAKGYLLKEGTAVDATLISAPSSTKNQSGERDPEMHQTKKGNQWHFGMKAHIGVD
HHHHHHCCCEEEECCCEECHHEEECCCCCCCCCCCCCCHHHHCCCCCCEECCEEEEECCC
ADSGLVHTVVGTAANVNDVTQAHALVHGKEADVFGDAGYQGIDKREEVQKLKVRWHVALR
CCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHEEEEEC
PGKRRALDKNTALGKLVDELERVKARIRARAEHPFRVIKRQFGHLKVRYRGLMKNTQQLH
CCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
TLFALSNLWMMRGRLMGEARA
HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKQTDLGLDLSNRRTRKQVFLDEMERVVPWQAFLALIAPHAPTKATGRKPFPIEAMLRIH
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
LLQQWFGLSDVAMEEALYDVPLYRQFAGLGGMSRLPDRVSILRFRHLLERHKLAEQFLQT
HHHHHHCCHHHHHHHHHHCCHHHHHHHCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHH
VNEQLSAKGYLLKEGTAVDATLISAPSSTKNQSGERDPEMHQTKKGNQWHFGMKAHIGVD
HHHHHHCCCEEEECCCEECHHEEECCCCCCCCCCCCCCHHHHCCCCCCEECCEEEEECCC
ADSGLVHTVVGTAANVNDVTQAHALVHGKEADVFGDAGYQGIDKREEVQKLKVRWHVALR
CCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHEEEEEC
PGKRRALDKNTALGKLVDELERVKARIRARAEHPFRVIKRQFGHLKVRYRGLMKNTQQLH
CCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
TLFALSNLWMMRGRLMGEARA
HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9097039; 9097040; 9278503 [H]