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The map label for this gene is greA

Identifier: 17546241

GI number: 17546241

Start: 1626430

End: 1626906

Strand: Direct

Name: greA

Synonym: RSc1522

Alternate gene names: 17546241

Gene position: 1626430-1626906 (Clockwise)

Preceding gene: 17546240

Following gene: 17546242

Centisome position: 43.76

GC content: 63.52

Gene sequence:

>477_bases
ATGAGCACCATTCCGATTACCAAGCGCGGTGCCGAGATGCTCAAGGATGAGCTGCAACGCCTGAAGACCAAGGAGCGTCC
AGCCGTTGTCAACGCCATCGCCGAAGCGCGTGCCCAGGGCGATCTGTCCGAAAACGCCGACTACGACGCCGCCAAGGAGC
GCCAGGGCTTCATCGAGGGCCGCATCCTCGAGATCGAGTCCAAGCTGGCTGCTGCGCAGGTCATCGACCCGGCAGGGCTG
GATGCCGATGGCCGCATCGTCTTCGGCGCCACCATCGACCTGGAAGATCTGGACTCCGGCAAGCCGGTCACCTACCAGAT
CGTCGGCGACGATGAAGCCGATCTGGATAGCGGCAAGATCTCGATCAGCTCGCCGATTGCGCGCGCGCTGATCGGTAAGT
ACGAAGGCGATGTCGCCACCGTGGTGGCGCCGGGCGGCGAACGCGAATACGAAGTGCGCGCGGTCAAGTACCTCTGA

Upstream 100 bases:

>100_bases
GTCCGTGTCAAAACAACGACAGCCGCCGTCAGGCGGGTGTGCGCTACGCGCCCTCCCGCTTCGCAGCGGCTGATTTTTTT
GTGATTCTGAATCGAACAAC

Downstream 100 bases:

>100_bases
CACCTCTGACAGAACGGAGCCCCACCATGCCGCAGCGCCTCTTCCAGTTGCTTGCCACGGTCTGGTGCGGATCGCTCTGG
ACCATCGGCTACATCGTTGC

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MSTIPITKRGAEMLKDELQRLKTKERPAVVNAIAEARAQGDLSENADYDAAKERQGFIEGRILEIESKLAAAQVIDPAGL
DADGRIVFGATIDLEDLDSGKPVTYQIVGDDEADLDSGKISISSPIARALIGKYEGDVATVVAPGGEREYEVRAVKYL

Sequences:

>Translated_158_residues
MSTIPITKRGAEMLKDELQRLKTKERPAVVNAIAEARAQGDLSENADYDAAKERQGFIEGRILEIESKLAAAQVIDPAGL
DADGRIVFGATIDLEDLDSGKPVTYQIVGDDEADLDSGKISISSPIARALIGKYEGDVATVVAPGGEREYEVRAVKYL
>Mature_157_residues
STIPITKRGAEMLKDELQRLKTKERPAVVNAIAEARAQGDLSENADYDAAKERQGFIEGRILEIESKLAAAQVIDPAGLD
ADGRIVFGATIDLEDLDSGKPVTYQIVGDDEADLDSGKISISSPIARALIGKYEGDVATVVAPGGEREYEVRAVKYL

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=158, Percent_Identity=56.9620253164557, Blast_Score=179, Evalue=5e-47,
Organism=Escherichia coli, GI87082261, Length=158, Percent_Identity=38.6075949367089, Blast_Score=100, Evalue=3e-23,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_RALSO (Q8XZ82)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_519643.1
- ProteinModelPortal:   Q8XZ82
- SMR:   Q8XZ82
- GeneID:   1220351
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc1522
- NMPDR:   fig|267608.1.peg.1522
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- BioCyc:   RSOL267608:RSC1522-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 16976; Mature: 16845

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIPITKRGAEMLKDELQRLKTKERPAVVNAIAEARAQGDLSENADYDAAKERQGFIEG
CCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCC
RILEIESKLAAAQVIDPAGLDADGRIVFGATIDLEDLDSGKPVTYQIVGDDEADLDSGKI
EEEEEHHHHHHHHHCCCCCCCCCCCEEEEEEEEHHHCCCCCCEEEEEECCCCCCCCCCEE
SISSPIARALIGKYEGDVATVVAPGGEREYEVRAVKYL
EECCHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEECC
>Mature Secondary Structure 
STIPITKRGAEMLKDELQRLKTKERPAVVNAIAEARAQGDLSENADYDAAKERQGFIEG
CCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCC
RILEIESKLAAAQVIDPAGLDADGRIVFGATIDLEDLDSGKPVTYQIVGDDEADLDSGKI
EEEEEHHHHHHHHHCCCCCCCCCCCEEEEEEEEHHHCCCCCCEEEEEECCCCCCCCCCEE
SISSPIARALIGKYEGDVATVVAPGGEREYEVRAVKYL
EECCHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852