BacMap

Definition	Ralstonia solanacearum GMI1000, complete genome.
Accession	NC_003295
Length	3,716,413

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The map label for this gene is yqhE [C]

Identifier: 17546219

GI number: 17546219

Start: 1604927

End: 1605757

Strand: Direct

Name: yqhE [C]

Synonym: RSc1500

Alternate gene names: 17546219

Gene position: 1604927-1605757 (Clockwise)

Preceding gene: 17546218

Following gene: 17546220

Centisome position: 43.18

GC content: 70.76

Gene sequence:

>831_bases
GTGACCGACACGCAGCCGGCCGTTACGCTCGGCGACGGGGCACGGATGCCCCAGCTCGGGCTGGGCCTCTGGCGCACGCC
GGCCGGGGAGACCGCCGGGCTTGTGCGGACGGCCATCGAGGCCGGCTATCGGCTGGTCGACACCGCCGCCGCGTACGGCA
ACGAGCGCGGGGTCGGCGAAGGCGTGCGCGCGGCTGGGGCGGAGCCAGCGGTCTTCGTCACCACGAAGCTCTGGAACGAG
GACCAGGGCTACGACCGCACCCTGCGCGCCTTCGATGAAAGCCTGCGATGCCTGGGACTACCCGCCATCGACCTCTACCT
GATCCACTGGCCCGCACCCTCACGGGGGCGCTATGTCGAAAGCTGGCGGGCACTGATCCGGCTCAGGGACGAGGGGCGGG
CGCGTTCGATCGGGGTGTCGAACTTCGGCGCCGAGCACTTGGCGCGGATCATCGATGAGACCGGCGAAGCCCCCGCGGTG
AACCAGATCGAGCTGCATCCGTGGTTTCAGCAGCGCGCGCTGCGCGCCTTCCACGCGCGCCACGGCGTGCGGACGGAATC
GTGGAGCCCGCTGGGCCAAGGCGGGCTCCTGCACGACCTCACCGTCCGGGAGATCGCCGCCCGCCATGGCAAGACGCCGG
CCCAGGTTGTCATACGCTGGCACTTGGACAACGGGCTGATCGTGATCCCGAAGTCCGCGCACCCGGACCGGATCCGCGAG
AACATCGCTGTTTTCGACTTCTGCCTGACGCCGGCGGACCAGGCCGCGATCGCGGGCCTCGATCGCGCGGATGGCCGTAT
CGGGCCCGATCCCGTGGCGGCCGGCTTCTAG

Upstream 100 bases:

>100_bases
TGCTGCTGGGCGAGGATCGGCCGCACCGCGTGGCGCGGGTCGACCAGAACGCCGGTGCCCATGTGCGCGTCAGCATCGCC
GGCTTCTTCCTGCGCGCGCC

Downstream 100 bases:

>100_bases
CCAGTCCGGCGCAGGCCCGTCTCTGGCGCGCCTTCGCGCATGCGTTGGGGTTTTCGCTAGTCCCTGCTTTTTTGACAGAC
CTGCTGCCTTGGTCCATGGT

Product: oxidoreductase

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MTDTQPAVTLGDGARMPQLGLGLWRTPAGETAGLVRTAIEAGYRLVDTAAAYGNERGVGEGVRAAGAEPAVFVTTKLWNE
DQGYDRTLRAFDESLRCLGLPAIDLYLIHWPAPSRGRYVESWRALIRLRDEGRARSIGVSNFGAEHLARIIDETGEAPAV
NQIELHPWFQQRALRAFHARHGVRTESWSPLGQGGLLHDLTVREIAARHGKTPAQVVIRWHLDNGLIVIPKSAHPDRIRE
NIAVFDFCLTPADQAAIAGLDRADGRIGPDPVAAGF

Sequences:

>Translated_276_residues
MTDTQPAVTLGDGARMPQLGLGLWRTPAGETAGLVRTAIEAGYRLVDTAAAYGNERGVGEGVRAAGAEPAVFVTTKLWNE
DQGYDRTLRAFDESLRCLGLPAIDLYLIHWPAPSRGRYVESWRALIRLRDEGRARSIGVSNFGAEHLARIIDETGEAPAV
NQIELHPWFQQRALRAFHARHGVRTESWSPLGQGGLLHDLTVREIAARHGKTPAQVVIRWHLDNGLIVIPKSAHPDRIRE
NIAVFDFCLTPADQAAIAGLDRADGRIGPDPVAAGF
>Mature_275_residues
TDTQPAVTLGDGARMPQLGLGLWRTPAGETAGLVRTAIEAGYRLVDTAAAYGNERGVGEGVRAAGAEPAVFVTTKLWNED
QGYDRTLRAFDESLRCLGLPAIDLYLIHWPAPSRGRYVESWRALIRLRDEGRARSIGVSNFGAEHLARIIDETGEAPAVN
QIELHPWFQQRALRAFHARHGVRTESWSPLGQGGLLHDLTVREIAARHGKTPAQVVIRWHLDNGLIVIPKSAHPDRIREN
IAVFDFCLTPADQAAIAGLDRADGRIGPDPVAAGF

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI223468663, Length=292, Percent_Identity=37.3287671232877, Blast_Score=198, Evalue=4e-51,
Organism=Homo sapiens, GI24497577, Length=290, Percent_Identity=34.1379310344828, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI5174391, Length=290, Percent_Identity=34.1379310344828, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI93277124, Length=301, Percent_Identity=35.2159468438538, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI310109922, Length=283, Percent_Identity=35.6890459363958, Blast_Score=172, Evalue=2e-43,
Organism=Homo sapiens, GI4502049, Length=288, Percent_Identity=36.8055555555556, Blast_Score=171, Evalue=7e-43,
Organism=Homo sapiens, GI291291012, Length=271, Percent_Identity=35.0553505535055, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI45446745, Length=301, Percent_Identity=33.8870431893688, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI4503285, Length=301, Percent_Identity=33.8870431893688, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI310109920, Length=301, Percent_Identity=33.8870431893688, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI5453543, Length=301, Percent_Identity=33.8870431893688, Blast_Score=168, Evalue=4e-42,
Organism=Homo sapiens, GI24497583, Length=313, Percent_Identity=33.2268370607029, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI5174695, Length=295, Percent_Identity=33.2203389830508, Blast_Score=164, Evalue=7e-41,
Organism=Homo sapiens, GI24497585, Length=294, Percent_Identity=33.6734693877551, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI300116273, Length=277, Percent_Identity=33.2129963898917, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI300116271, Length=275, Percent_Identity=32, Blast_Score=131, Evalue=7e-31,
Organism=Homo sapiens, GI310109924, Length=149, Percent_Identity=36.9127516778523, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI207028673, Length=123, Percent_Identity=35.7723577235772, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI310109926, Length=120, Percent_Identity=36.6666666666667, Blast_Score=78, Evalue=8e-15,
Organism=Escherichia coli, GI87082198, Length=272, Percent_Identity=51.4705882352941, Blast_Score=281, Evalue=3e-77,
Organism=Escherichia coli, GI1786400, Length=261, Percent_Identity=36.7816091954023, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1788081, Length=287, Percent_Identity=29.9651567944251, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87081735, Length=290, Percent_Identity=24.1379310344828, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17537077, Length=290, Percent_Identity=37.5862068965517, Blast_Score=184, Evalue=6e-47,
Organism=Caenorhabditis elegans, GI17537075, Length=299, Percent_Identity=37.123745819398, Blast_Score=182, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI71998625, Length=269, Percent_Identity=32.7137546468402, Blast_Score=173, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI17550248, Length=290, Percent_Identity=33.7931034482759, Blast_Score=164, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17564128, Length=297, Percent_Identity=32.6599326599327, Blast_Score=163, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI17566692, Length=297, Percent_Identity=30.6397306397306, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17537079, Length=300, Percent_Identity=31.3333333333333, Blast_Score=151, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17561298, Length=275, Percent_Identity=30.5454545454545, Blast_Score=146, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17538386, Length=270, Percent_Identity=31.4814814814815, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17552492, Length=259, Percent_Identity=29.7297297297297, Blast_Score=130, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17561300, Length=253, Percent_Identity=27.6679841897233, Blast_Score=124, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17562292, Length=280, Percent_Identity=28.5714285714286, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324694, Length=297, Percent_Identity=34.3434343434343, Blast_Score=183, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6320576, Length=296, Percent_Identity=31.7567567567568, Blast_Score=171, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6322556, Length=251, Percent_Identity=35.4581673306773, Blast_Score=169, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6321896, Length=303, Percent_Identity=32.013201320132, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6319625, Length=278, Percent_Identity=34.5323741007194, Blast_Score=151, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6320079, Length=287, Percent_Identity=28.9198606271777, Blast_Score=115, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24657054, Length=279, Percent_Identity=37.2759856630824, Blast_Score=181, Evalue=6e-46,
Organism=Drosophila melanogaster, GI20129731, Length=289, Percent_Identity=36.3321799307958, Blast_Score=178, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24663317, Length=286, Percent_Identity=36.013986013986, Blast_Score=175, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24644950, Length=306, Percent_Identity=34.9673202614379, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24662789, Length=308, Percent_Identity=34.4155844155844, Blast_Score=171, Evalue=4e-43,
Organism=Drosophila melanogaster, GI21356425, Length=293, Percent_Identity=32.4232081911263, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24662781, Length=297, Percent_Identity=37.037037037037, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI221378297, Length=326, Percent_Identity=32.8220858895706, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24662785, Length=297, Percent_Identity=37.037037037037, Blast_Score=164, Evalue=6e-41,
Organism=Drosophila melanogaster, GI45553081, Length=279, Percent_Identity=34.0501792114695, Blast_Score=154, Evalue=9e-38,
Organism=Drosophila melanogaster, GI281366140, Length=243, Percent_Identity=33.7448559670782, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 30125; Mature: 29994

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTQPAVTLGDGARMPQLGLGLWRTPAGETAGLVRTAIEAGYRLVDTAAAYGNERGVGE
CCCCCCCEEECCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCC
GVRAAGAEPAVFVTTKLWNEDQGYDRTLRAFDESLRCLGLPAIDLYLIHWPAPSRGRYVE
CHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHH
SWRALIRLRDEGRARSIGVSNFGAEHLARIIDETGEAPAVNQIELHPWFQQRALRAFHAR
HHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHH
HGVRTESWSPLGQGGLLHDLTVREIAARHGKTPAQVVIRWHLDNGLIVIPKSAHPDRIRE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHEEEEEEECCCEEEEECCCCHHHHHH
NIAVFDFCLTPADQAAIAGLDRADGRIGPDPVAAGF
HHEEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDTQPAVTLGDGARMPQLGLGLWRTPAGETAGLVRTAIEAGYRLVDTAAAYGNERGVGE
CCCCCCEEECCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCC
GVRAAGAEPAVFVTTKLWNEDQGYDRTLRAFDESLRCLGLPAIDLYLIHWPAPSRGRYVE
CHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHH
SWRALIRLRDEGRARSIGVSNFGAEHLARIIDETGEAPAVNQIELHPWFQQRALRAFHAR
HHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHH
HGVRTESWSPLGQGGLLHDLTVREIAARHGKTPAQVVIRWHLDNGLIVIPKSAHPDRIRE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHEEEEEEECCCEEEEECCCCHHHHHH
NIAVFDFCLTPADQAAIAGLDRADGRIGPDPVAAGF
HHEEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA