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The map label for this gene is smpB [H]

Identifier: 17546143

GI number: 17546143

Start: 1524926

End: 1525372

Strand: Reverse

Name: smpB [H]

Synonym: RSc1424

Alternate gene names: 17546143

Gene position: 1525372-1524926 (Counterclockwise)

Preceding gene: 17546146

Following gene: 17546139

Centisome position: 41.04

GC content: 63.31

Gene sequence:

>447_bases
ATGACCATCGCCGACAACAAGAAAGCCTTTTTCGATTACTTCGTCGAAGAGCGCTATGAGGCGGGCATCGCACTCGAAGG
CTGGGAGGTGAAGGCCATCCGCGCCGGCCGCGTGCAGATCAAGGAAGGCTACGTCGTCATTCGCGACGCCGAACTGTTCC
TGATCGGGGCACACATCAGCCCGCTGCAATCCGCCTCCACGCACGTCAAGCCCGACCCGACGCGCACCCGCAAGCTGCTG
CTGCACGCCGAAGAGATCAAGAAGCTGATCGGCAAGGTCGAGCAGCGCGGCTATACGCTCGTGCCGCTCAACCTGCACTA
TGCGCGCGGCCGCGTGAAGTGCGAGATCGGCCTGGCCAAGGGCAAGAAGCTCTACGACAAGCGCGAGACCGAGAAGGACC
GCGACTGGCAGCGCGAGAAGGCGCGGCTCATGCGCGAGAAGGCCTGA

Upstream 100 bases:

>100_bases
CACCGCATTCCTGTCGTTGGCCCGCTTGGGCCTGCGGCGGATTTGTTAAACTCGTCATTTTATCGCAGCGCATCCCACGC
GCTAACCGCCACATCCCAGT

Downstream 100 bases:

>100_bases
CCCGCCTCGCGCCTCATCGCATCGCTACGCGTTCGGCCGCTGCTGCTTCACGATCTGCAGCGCCGACGCGATCATCGTGC
TCAGGTCGCCCAGGTTGCCG

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MTIADNKKAFFDYFVEERYEAGIALEGWEVKAIRAGRVQIKEGYVVIRDAELFLIGAHISPLQSASTHVKPDPTRTRKLL
LHAEEIKKLIGKVEQRGYTLVPLNLHYARGRVKCEIGLAKGKKLYDKRETEKDRDWQREKARLMREKA

Sequences:

>Translated_148_residues
MTIADNKKAFFDYFVEERYEAGIALEGWEVKAIRAGRVQIKEGYVVIRDAELFLIGAHISPLQSASTHVKPDPTRTRKLL
LHAEEIKKLIGKVEQRGYTLVPLNLHYARGRVKCEIGLAKGKKLYDKRETEKDRDWQREKARLMREKA
>Mature_147_residues
TIADNKKAFFDYFVEERYEAGIALEGWEVKAIRAGRVQIKEGYVVIRDAELFLIGAHISPLQSASTHVKPDPTRTRKLLL
HAEEIKKLIGKVEQRGYTLVPLNLHYARGRVKCEIGLAKGKKLYDKRETEKDRDWQREKARLMREKA

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=145, Percent_Identity=54.4827586206897, Blast_Score=169, Evalue=8e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 17151; Mature: 17020

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIADNKKAFFDYFVEERYEAGIALEGWEVKAIRAGRVQIKEGYVVIRDAELFLIGAHIS
CCCCCCCHHHHHHHHHHHHHCCEEECCCEEEEEECCEEEEECCEEEEEECEEEEEECCCC
PLQSASTHVKPDPTRTRKLLLHAEEIKKLIGKVEQRGYTLVPLNLHYARGRVKCEIGLAK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEEEECCC
GKKLYDKRETEKDRDWQREKARLMREKA
CCHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIADNKKAFFDYFVEERYEAGIALEGWEVKAIRAGRVQIKEGYVVIRDAELFLIGAHIS
CCCCCCHHHHHHHHHHHHHCCEEECCCEEEEEECCEEEEECCEEEEEECEEEEEECCCC
PLQSASTHVKPDPTRTRKLLLHAEEIKKLIGKVEQRGYTLVPLNLHYARGRVKCEIGLAK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEEEECCC
GKKLYDKRETEKDRDWQREKARLMREKA
CCHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA