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The map label for this gene is sbp [H]

Identifier: 17546055

GI number: 17546055

Start: 1423811

End: 1424821

Strand: Direct

Name: sbp [H]

Synonym: RSc1336

Alternate gene names: 17546055

Gene position: 1423811-1424821 (Clockwise)

Preceding gene: 17546054

Following gene: 17546056

Centisome position: 38.31

GC content: 66.96

Gene sequence:

>1011_bases
ATGATTCGCAAGTTCGTGTTCAACGCTATCGCGGCGGCCATCGTGGCGGGGGGCACCGTGGGCGCGCACGCCGCCACGAC
GCTGCTGAACGTGTCGTATGACCCGACGCGCGAGCTGTACAAGGAGATCAACGCCGAGTTCGCCAAGAAGTGGAAGGCCG
AGACCGGTGAAGACGTGACCCTGCGCGCCTCGCACGGCGGCTCGGGCAAGCAGGCCCGCTCGGTCATCGACGGCCTGGAG
GCCGACGTGGTGACGCTGGCGCTTGGCTACGACGTCGACGCCATCGCCGAGAAGGGGCTGACGGGCAAGGACTGGCAGAC
GCGGCTGCCGCACAACGCGTCGCCGTACACCTCGACCATCGTGTTCCTGGTGCGCAAGGGCAACCCGAAGGGCATCAAGG
ACTGGAGCGACCTGGTCAAGCCGGGCATCGCCGTCATCACGCCCAATCCGAAGACCTCGGGCGGCGCCCGCTGGAACTAC
CTGGCCGCGTGGGCTTATGCGCTCAAGCAGCCGGGCGGCTCGGAAGCGACGGCCAAGGCGTTTGTGCAGAAGCTGTACAA
GAACGTGCCGGTGCTGGATTCCGGCGCGCGCGGCGCCACCACGACCTTCACCGAGCGCGGCATCGGCGATGTGCTGATCG
CGTGGGAAGACGAGGCGCTGCTGGCCACGCGCGTGGAGGGCAAGGACAAGTTCGATGTGGTGGTGCCGTCCGTCTCCATC
CTGGCCGAGCCGCCGGTGGCGGTGGTCGACAAGGTGGCCGACAAGAGGGGCACGCGCAAGGCGGCCGAGGCTTACCTCAA
GTTCCTGTATTCGCCGCAGGGGCAGGAGATCGGCGCGAAGCACTTCTACCGGCCGACCGACCCGGCCGTGGCCAGGAGGC
ACGAGAGCGAATTCCCGAAGGTGAAGCTGGTGACCATCGACGACACCTTCGGCGGCTGGCAGAAGGCGCAGAAGACGCAC
TTCGCCGATGGCGGCCAGTTCGACCAGCTCTATCAACCCGGCAGCAAGTAA

Upstream 100 bases:

>100_bases
TGAAACCATTCGTTCCCGGTAGAAGGGGGGCTCGGTAGTCTTGCTGCTATCCGAACAACGTGGCGCGGCCACGGACAACA
AAGCAACTGGAGCCAGCAAG

Downstream 100 bases:

>100_bases
GCTTCAGCCACGTCACCAGGCGGTCCGCATCATGCCCATCCTGACGATCTCCGGCAGCCCTTCGGCGCCATCGCGATCCG
CGCGCCTGCTGGGCCATGTG

Product: sulfate-binding precursor signal peptide protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MIRKFVFNAIAAAIVAGGTVGAHAATTLLNVSYDPTRELYKEINAEFAKKWKAETGEDVTLRASHGGSGKQARSVIDGLE
ADVVTLALGYDVDAIAEKGLTGKDWQTRLPHNASPYTSTIVFLVRKGNPKGIKDWSDLVKPGIAVITPNPKTSGGARWNY
LAAWAYALKQPGGSEATAKAFVQKLYKNVPVLDSGARGATTTFTERGIGDVLIAWEDEALLATRVEGKDKFDVVVPSVSI
LAEPPVAVVDKVADKRGTRKAAEAYLKFLYSPQGQEIGAKHFYRPTDPAVARRHESEFPKVKLVTIDDTFGGWQKAQKTH
FADGGQFDQLYQPGSK

Sequences:

>Translated_336_residues
MIRKFVFNAIAAAIVAGGTVGAHAATTLLNVSYDPTRELYKEINAEFAKKWKAETGEDVTLRASHGGSGKQARSVIDGLE
ADVVTLALGYDVDAIAEKGLTGKDWQTRLPHNASPYTSTIVFLVRKGNPKGIKDWSDLVKPGIAVITPNPKTSGGARWNY
LAAWAYALKQPGGSEATAKAFVQKLYKNVPVLDSGARGATTTFTERGIGDVLIAWEDEALLATRVEGKDKFDVVVPSVSI
LAEPPVAVVDKVADKRGTRKAAEAYLKFLYSPQGQEIGAKHFYRPTDPAVARRHESEFPKVKLVTIDDTFGGWQKAQKTH
FADGGQFDQLYQPGSK
>Mature_336_residues
MIRKFVFNAIAAAIVAGGTVGAHAATTLLNVSYDPTRELYKEINAEFAKKWKAETGEDVTLRASHGGSGKQARSVIDGLE
ADVVTLALGYDVDAIAEKGLTGKDWQTRLPHNASPYTSTIVFLVRKGNPKGIKDWSDLVKPGIAVITPNPKTSGGARWNY
LAAWAYALKQPGGSEATAKAFVQKLYKNVPVLDSGARGATTTFTERGIGDVLIAWEDEALLATRVEGKDKFDVVVPSVSI
LAEPPVAVVDKVADKRGTRKAAEAYLKFLYSPQGQEIGAKHFYRPTDPAVARRHESEFPKVKLVTIDDTFGGWQKAQKTH
FADGGQFDQLYQPGSK

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=303, Percent_Identity=69.6369636963696, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1788765, Length=316, Percent_Identity=49.0506329113924, Blast_Score=307, Evalue=6e-85,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36362; Mature: 36362

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRKFVFNAIAAAIVAGGTVGAHAATTLLNVSYDPTRELYKEINAEFAKKWKAETGEDVT
CHHHHHHHHHHHHHHCCCCCCHHHHHHEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEE
LRASHGGSGKQARSVIDGLEADVVTLALGYDVDAIAEKGLTGKDWQTRLPHNASPYTSTI
EEECCCCCCHHHHHHHCCCCCCEEEEEECCCHHHHHHCCCCCCCCHHCCCCCCCCCCEEE
VFLVRKGNPKGIKDWSDLVKPGIAVITPNPKTSGGARWNYLAAWAYALKQPGGSEATAKA
EEEEECCCCCCCCHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHH
FVQKLYKNVPVLDSGARGATTTFTERGIGDVLIAWEDEALLATRVEGKDKFDVVVPSVSI
HHHHHHHCCCCCCCCCCCCCCHHHHCCCCCEEEEECCCEEEEEECCCCCCEEEEECCHHH
LAEPPVAVVDKVADKRGTRKAAEAYLKFLYSPQGQEIGAKHFYRPTDPAVARRHESEFPK
HCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHCCCCE
VKLVTIDDTFGGWQKAQKTHFADGGQFDQLYQPGSK
EEEEEEECCCCCHHHHHHHCCCCCCCHHHHCCCCCC
>Mature Secondary Structure
MIRKFVFNAIAAAIVAGGTVGAHAATTLLNVSYDPTRELYKEINAEFAKKWKAETGEDVT
CHHHHHHHHHHHHHHCCCCCCHHHHHHEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEE
LRASHGGSGKQARSVIDGLEADVVTLALGYDVDAIAEKGLTGKDWQTRLPHNASPYTSTI
EEECCCCCCHHHHHHHCCCCCCEEEEEECCCHHHHHHCCCCCCCCHHCCCCCCCCCCEEE
VFLVRKGNPKGIKDWSDLVKPGIAVITPNPKTSGGARWNYLAAWAYALKQPGGSEATAKA
EEEEECCCCCCCCHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHH
FVQKLYKNVPVLDSGARGATTTFTERGIGDVLIAWEDEALLATRVEGKDKFDVVVPSVSI
HHHHHHHCCCCCCCCCCCCCCHHHHCCCCCEEEEECCCEEEEEECCCCCCEEEEECCHHH
LAEPPVAVVDKVADKRGTRKAAEAYLKFLYSPQGQEIGAKHFYRPTDPAVARRHESEFPK
HCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHCCCCE
VKLVTIDDTFGGWQKAQKTHFADGGQFDQLYQPGSK
EEEEEEECCCCCHHHHHHHCCCCCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]