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The map label for this gene is dusA [H]

Identifier: 17546018

GI number: 17546018

Start: 1385284

End: 1386318

Strand: Direct

Name: dusA [H]

Synonym: RSc1299

Alternate gene names: 17546018

Gene position: 1385284-1386318 (Clockwise)

Preceding gene: 17546017

Following gene: 17546019

Centisome position: 37.27

GC content: 68.7

Gene sequence:

>1035_bases
GTGTCTGCTGAACGCTCTACGCCGAATCCGCGTCGCCTGTCCGTTGCCCCGATGATGGACTGGACCGATCGCCACTGTCG
CTACTTCCATCGCCAGATCTCCCGCCACACCTGGCTCTACACGGAAATGGTGACCACCGGCGCGCTCCTGCACGGCGACG
TGCCGCGCCATCTCGACTTCGACGAGGCGGAGCACCCGGTTGCGCTGCAGCTCGGCGGCAGCGAACCCGACGACCTGGCG
CAGTCCGCCCGCTTGGGGGAGCGCTGGGGCTATCGCGAGATCAACCTGAACTGCGGCTGTCCGTCCGAGCGCGTGCAGCG
CGGTGCGTTCGGCGCGTGCCTGATGGCCGAGCCGAAGCTGGTGGCCGATGGCGTCAAGGCGATGCGCGATGCGGTGTCGA
TCCCGGTCACGGTCAAGCACCGTATCGGCATCGACGCGATTGAGACCTACGACTTCGTGCGCGATTTCGTCGGCGCCATC
GCCGAGGCGGGTTGCGAGACCTTCATCGTGCACGCCCGCAACGCCATCCTGAAGGGGTTGAGCCCGAAGGAGAACCGCGA
GATCCCGCCTCTGCGCTACGAGGTGGTCTACCAGCTCAAGCGTGATTTCCCGCGGCTGGAGATCCTGCTGAACGGCGGCG
TGCAGACGCTGGAGCAGATCGACACGCATCTTGCCCACGTCGACGGCGTGATGATCGGCCGCGAGGCCTATCACCACCCG
CACCTGATGGCGGGCTTCGATGCGCGCTACTACGGTGCCGACGCGGTCGCGCCCACGCGCGCCGAGGTCGAAGACGCAAT
GATCGATTACATCGGCCGCTGGGTGGCGCGGGGCGGATATGCCGGCGCAGCGGTGCGCCACATGCTGGGGCTGTATCGCG
GCGTGGCCGGCGCCCGGGGCTGGCGGCGCGTGCTGTCGGACGCCAAGGCGCTTGGCCGCGTCAAGACGCCGGACGACGCG
CGAGCCCTGTTCGAGACGGCGCGCGCGCACCTGCGCCGCGGCGCGGAGGCGGATGAAGAGGCGGTCGCCCCATAA

Upstream 100 bases:

>100_bases
GTTCTTCGCATCGCCGTGGCGGCGCGAGCAGCATCCGCTGCTGGGGCTGTCGGACTAGAACCGGTAAACTTGCGCGCCTG
AACCGAATTACCGAATTGCC

Downstream 100 bases:

>100_bases
ATATTGCGCAGCCCTCTAGTCACGTCAGAAGAATGTTTGTATAATTTTGTTTTTCGCCGAGCAGCGGTTCGCTGTTCGAA
CGACGGTGGCTGTAGCTCAG

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSAERSTPNPRRLSVAPMMDWTDRHCRYFHRQISRHTWLYTEMVTTGALLHGDVPRHLDFDEAEHPVALQLGGSEPDDLA
QSARLGERWGYREINLNCGCPSERVQRGAFGACLMAEPKLVADGVKAMRDAVSIPVTVKHRIGIDAIETYDFVRDFVGAI
AEAGCETFIVHARNAILKGLSPKENREIPPLRYEVVYQLKRDFPRLEILLNGGVQTLEQIDTHLAHVDGVMIGREAYHHP
HLMAGFDARYYGADAVAPTRAEVEDAMIDYIGRWVARGGYAGAAVRHMLGLYRGVAGARGWRRVLSDAKALGRVKTPDDA
RALFETARAHLRRGAEADEEAVAP

Sequences:

>Translated_344_residues
MSAERSTPNPRRLSVAPMMDWTDRHCRYFHRQISRHTWLYTEMVTTGALLHGDVPRHLDFDEAEHPVALQLGGSEPDDLA
QSARLGERWGYREINLNCGCPSERVQRGAFGACLMAEPKLVADGVKAMRDAVSIPVTVKHRIGIDAIETYDFVRDFVGAI
AEAGCETFIVHARNAILKGLSPKENREIPPLRYEVVYQLKRDFPRLEILLNGGVQTLEQIDTHLAHVDGVMIGREAYHHP
HLMAGFDARYYGADAVAPTRAEVEDAMIDYIGRWVARGGYAGAAVRHMLGLYRGVAGARGWRRVLSDAKALGRVKTPDDA
RALFETARAHLRRGAEADEEAVAP
>Mature_343_residues
SAERSTPNPRRLSVAPMMDWTDRHCRYFHRQISRHTWLYTEMVTTGALLHGDVPRHLDFDEAEHPVALQLGGSEPDDLAQ
SARLGERWGYREINLNCGCPSERVQRGAFGACLMAEPKLVADGVKAMRDAVSIPVTVKHRIGIDAIETYDFVRDFVGAIA
EAGCETFIVHARNAILKGLSPKENREIPPLRYEVVYQLKRDFPRLEILLNGGVQTLEQIDTHLAHVDGVMIGREAYHHPH
LMAGFDARYYGADAVAPTRAEVEDAMIDYIGRWVARGGYAGAAVRHMLGLYRGVAGARGWRRVLSDAKALGRVKTPDDAR
ALFETARAHLRRGAEADEEAVAP

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=240, Percent_Identity=27.5, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI40807366, Length=244, Percent_Identity=27.4590163934426, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI145693211, Length=297, Percent_Identity=60.2693602693603, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1788462, Length=181, Percent_Identity=31.4917127071823, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789660, Length=134, Percent_Identity=30.5970149253731, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25144369, Length=214, Percent_Identity=28.0373831775701, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=238, Percent_Identity=24.3697478991597, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24580595, Length=251, Percent_Identity=23.9043824701195, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19920448, Length=251, Percent_Identity=23.9043824701195, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 38312; Mature: 38181

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAERSTPNPRRLSVAPMMDWTDRHCRYFHRQISRHTWLYTEMVTTGALLHGDVPRHLDF
CCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DEAEHPVALQLGGSEPDDLAQSARLGERWGYREINLNCGCPSERVQRGAFGACLMAEPKL
CCCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHCCCCCEEEECCCHH
VADGVKAMRDAVSIPVTVKHRIGIDAIETYDFVRDFVGAIAEAGCETFIVHARNAILKGL
HHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
SPKENREIPPLRYEVVYQLKRDFPRLEILLNGGVQTLEQIDTHLAHVDGVMIGREAYHHP
CCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHEECHHHHCCC
HLMAGFDARYYGADAVAPTRAEVEDAMIDYIGRWVARGGYAGAAVRHMLGLYRGVAGARG
CEEECCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
WRRVLSDAKALGRVKTPDDARALFETARAHLRRGAEADEEAVAP
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCC
>Mature Secondary Structure 
SAERSTPNPRRLSVAPMMDWTDRHCRYFHRQISRHTWLYTEMVTTGALLHGDVPRHLDF
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DEAEHPVALQLGGSEPDDLAQSARLGERWGYREINLNCGCPSERVQRGAFGACLMAEPKL
CCCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHCCCCCEEEECCCHH
VADGVKAMRDAVSIPVTVKHRIGIDAIETYDFVRDFVGAIAEAGCETFIVHARNAILKGL
HHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
SPKENREIPPLRYEVVYQLKRDFPRLEILLNGGVQTLEQIDTHLAHVDGVMIGREAYHHP
CCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHEECHHHHCCC
HLMAGFDARYYGADAVAPTRAEVEDAMIDYIGRWVARGGYAGAAVRHMLGLYRGVAGARG
CEEECCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
WRRVLSDAKALGRVKTPDDARALFETARAHLRRGAEADEEAVAP
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]