Click here to switch to the map view.

The map label for this gene is glnA1 [H]

Identifier: 17545977

GI number: 17545977

Start: 1336769

End: 1338184

Strand: Direct

Name: glnA1 [H]

Synonym: RSc1258

Alternate gene names: 17545977

Gene position: 1336769-1338184 (Clockwise)

Preceding gene: 17545975

Following gene: 17545978

Centisome position: 35.97

GC content: 61.58

Gene sequence:

>1416_bases
ATGTCCCAAAGCATTGCAGACGTGATGAAGCTCGTGAAGGAAAACGACGTCAAGTTCGTCGATTTCCGCTTCACCGATAC
CAAGGGTAAAGAGCAGCACGTCTCGGTTCCCGTGTCGCATTTCGGCGAAGACAAGTTCGAAAGCGGCCACGCATTCGACG
GCTCGTCGATCGCCGGCTGGAAGGGCATCGAAGCTTCGGACATGCTGCTGATGCCGGATGCGAACACCGCCCACATCGAC
CCGTTCTACGAAGAGCCGACCCTGGTCATGACCTGCGACGTGATCGAACCATCGGACGGCAAGGGCTACGACCGCGACCC
GCGCTCGATCGCCAAGCGCGCTGAAGCCTACCTGAAGAGCACCGGCCTGGGCGACACCGCCTACTTCGGTCCGGAACCCG
AATTCTTCATCTTCGACGGCGTGACCTGGAACGTCGACATGCAAGGCTCCTTCGTGAAGATCCATTCCGAAGAAGCGCCG
TGGTCGTCGGGCAAGGAATTCGAGCACGGCAACTCCGGCCACCGTCCGGGCAAGAAGGGCGGCTACTTCCCGGTCGCCCC
GATCGACACGTTCCAGGACATCCGCTCGGAAATGTGCCTGATCCTGGAATCGCTGGGCATTCCGGTTGAAGTGCATCACC
ACGAAGTGGCGGGCCAAGGCCAGAACGAAATCGGCACGAAGTTCAGCACGCTGGTGCAGCGCGCCGACTGGACCCAGCTG
CAAAAGTACGTGATCCAGAACGTCGCGCACACCTACGGCAAGACCGCCACGTTCATGCCGAAGCCGGTCGTGGGCGACAA
CGGTTCGGGTATGCACGTGCACCAGTCCGTGTGGAAGGACGGCCAGAACCTGTTCGCAGGCAACGGCTACGCCGGCCTGT
CGGAATTCGCGCTGTACTACATCGGCGGCATCATCAAGCACGCTCGCGCGCTGAACGCCATCACCAACCCGGGCACGAAC
TCGTACAAGCGTCTGGTGCCGGGCTTCGAAGCACCGGTCAAGCTGGCTTACTCGGCTCGCAACCGTTCGGCTTCGATCCG
GATCCCGTACGTTGCCAACCCGAAGGGTCGCCGCATCGAAACGCGCTTCCCGGATCCGCTGTGCAACCCGTACCTGGGCT
TTGCCGCGCTGCTGATGGCCGGCCTGGACGGCGTGCAGAACAAGATCCACCCGGGCGAAGCCGCCGACAAGAACCTGTAC
GACCTGCCGCCGGAAGAGGACGCAAAGATCCCGACCGTGTGCTCGAGCCTGGATCAGGCCCTCGAGTACCTGGACAAGGA
TCGCGAGTTCCTGACCCGCGGCGGCGTGTTCACCAACTCGATGATCGACGCCTACATCGAGCTGAAGATGGAAGAAGTCA
CCCGCTTCCGCATGACCACGCACCCGGTCGAGTTCGAGATGTACTACTCGCTGTAA

Upstream 100 bases:

>100_bases
CTGCTAGGAAGAAGATGTTCCGGTGCGTGGGGGCGGGGCGCTACGATCCGTCACACAACCGGCTCACAATGTTGCGATAT
AGCGCTACCAGGAGAATGGC

Downstream 100 bases:

>100_bases
CAGGCGGGCGCCCCGCGCGATGCGGCAGCGGGGCGCGGATGCGACAGTGTTGAGGGGTAAGGGCGGCGCAGGTCGCCCTT
CCTTTTTGTGAAGCGGGGGA

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSQSIADVMKLVKENDVKFVDFRFTDTKGKEQHVSVPVSHFGEDKFESGHAFDGSSIAGWKGIEASDMLLMPDANTAHID
PFYEEPTLVMTCDVIEPSDGKGYDRDPRSIAKRAEAYLKSTGLGDTAYFGPEPEFFIFDGVTWNVDMQGSFVKIHSEEAP
WSSGKEFEHGNSGHRPGKKGGYFPVAPIDTFQDIRSEMCLILESLGIPVEVHHHEVAGQGQNEIGTKFSTLVQRADWTQL
QKYVIQNVAHTYGKTATFMPKPVVGDNGSGMHVHQSVWKDGQNLFAGNGYAGLSEFALYYIGGIIKHARALNAITNPGTN
SYKRLVPGFEAPVKLAYSARNRSASIRIPYVANPKGRRIETRFPDPLCNPYLGFAALLMAGLDGVQNKIHPGEAADKNLY
DLPPEEDAKIPTVCSSLDQALEYLDKDREFLTRGGVFTNSMIDAYIELKMEEVTRFRMTTHPVEFEMYYSL

Sequences:

>Translated_471_residues
MSQSIADVMKLVKENDVKFVDFRFTDTKGKEQHVSVPVSHFGEDKFESGHAFDGSSIAGWKGIEASDMLLMPDANTAHID
PFYEEPTLVMTCDVIEPSDGKGYDRDPRSIAKRAEAYLKSTGLGDTAYFGPEPEFFIFDGVTWNVDMQGSFVKIHSEEAP
WSSGKEFEHGNSGHRPGKKGGYFPVAPIDTFQDIRSEMCLILESLGIPVEVHHHEVAGQGQNEIGTKFSTLVQRADWTQL
QKYVIQNVAHTYGKTATFMPKPVVGDNGSGMHVHQSVWKDGQNLFAGNGYAGLSEFALYYIGGIIKHARALNAITNPGTN
SYKRLVPGFEAPVKLAYSARNRSASIRIPYVANPKGRRIETRFPDPLCNPYLGFAALLMAGLDGVQNKIHPGEAADKNLY
DLPPEEDAKIPTVCSSLDQALEYLDKDREFLTRGGVFTNSMIDAYIELKMEEVTRFRMTTHPVEFEMYYSL
>Mature_470_residues
SQSIADVMKLVKENDVKFVDFRFTDTKGKEQHVSVPVSHFGEDKFESGHAFDGSSIAGWKGIEASDMLLMPDANTAHIDP
FYEEPTLVMTCDVIEPSDGKGYDRDPRSIAKRAEAYLKSTGLGDTAYFGPEPEFFIFDGVTWNVDMQGSFVKIHSEEAPW
SSGKEFEHGNSGHRPGKKGGYFPVAPIDTFQDIRSEMCLILESLGIPVEVHHHEVAGQGQNEIGTKFSTLVQRADWTQLQ
KYVIQNVAHTYGKTATFMPKPVVGDNGSGMHVHQSVWKDGQNLFAGNGYAGLSEFALYYIGGIIKHARALNAITNPGTNS
YKRLVPGFEAPVKLAYSARNRSASIRIPYVANPKGRRIETRFPDPLCNPYLGFAALLMAGLDGVQNKIHPGEAADKNLYD
LPPEEDAKIPTVCSSLDQALEYLDKDREFLTRGGVFTNSMIDAYIELKMEEVTRFRMTTHPVEFEMYYSL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=464, Percent_Identity=66.3793103448276, Blast_Score=665, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=465, Percent_Identity=24.5161290322581, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52377; Mature: 52245

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSIADVMKLVKENDVKFVDFRFTDTKGKEQHVSVPVSHFGEDKFESGHAFDGSSIAGW
CCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCHHHCCCCCCCCCCCCCCCCCCCC
KGIEASDMLLMPDANTAHIDPFYEEPTLVMTCDVIEPSDGKGYDRDPRSIAKRAEAYLKS
CCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
TGLGDTAYFGPEPEFFIFDGVTWNVDMQGSFVKIHSEEAPWSSGKEFEHGNSGHRPGKKG
CCCCCCCCCCCCCCEEEECCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
GYFPVAPIDTFQDIRSEMCLILESLGIPVEVHHHEVAGQGQNEIGTKFSTLVQRADWTQL
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHH
QKYVIQNVAHTYGKTATFMPKPVVGDNGSGMHVHQSVWKDGQNLFAGNGYAGLSEFALYY
HHHHHHHHHHHHCCCEECCCCCCCCCCCCCCEEHHHHHHCCCCEECCCCCCCHHHHHHHH
IGGIIKHARALNAITNPGTNSYKRLVPGFEAPVKLAYSARNRSASIRIPYVANPKGRRIE
HHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCEEE
TRFPDPLCNPYLGFAALLMAGLDGVQNKIHPGEAADKNLYDLPPEEDAKIPTVCSSLDQA
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LEYLDKDREFLTRGGVFTNSMIDAYIELKMEEVTRFRMTTHPVEFEMYYSL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
>Mature Secondary Structure 
SQSIADVMKLVKENDVKFVDFRFTDTKGKEQHVSVPVSHFGEDKFESGHAFDGSSIAGW
CCHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCHHHCCCCCCCCCCCCCCCCCCCC
KGIEASDMLLMPDANTAHIDPFYEEPTLVMTCDVIEPSDGKGYDRDPRSIAKRAEAYLKS
CCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
TGLGDTAYFGPEPEFFIFDGVTWNVDMQGSFVKIHSEEAPWSSGKEFEHGNSGHRPGKKG
CCCCCCCCCCCCCCEEEECCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
GYFPVAPIDTFQDIRSEMCLILESLGIPVEVHHHEVAGQGQNEIGTKFSTLVQRADWTQL
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHH
QKYVIQNVAHTYGKTATFMPKPVVGDNGSGMHVHQSVWKDGQNLFAGNGYAGLSEFALYY
HHHHHHHHHHHHCCCEECCCCCCCCCCCCCCEEHHHHHHCCCCEECCCCCCCHHHHHHHH
IGGIIKHARALNAITNPGTNSYKRLVPGFEAPVKLAYSARNRSASIRIPYVANPKGRRIE
HHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCEEE
TRFPDPLCNPYLGFAALLMAGLDGVQNKIHPGEAADKNLYDLPPEEDAKIPTVCSSLDQA
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LEYLDKDREFLTRGGVFTNSMIDAYIELKMEEVTRFRMTTHPVEFEMYYSL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1969923 [H]