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The map label for this gene is trmJ [H]

Identifier: 17545880

GI number: 17545880

Start: 1219971

End: 1220834

Strand: Direct

Name: trmJ [H]

Synonym: RSc1161

Alternate gene names: 17545880

Gene position: 1219971-1220834 (Clockwise)

Preceding gene: 17545878

Following gene: 17545881

Centisome position: 32.83

GC content: 70.95

Gene sequence:

>864_bases
ATGAACCCCGCCTCCCGCGCCTCGGAAACCAGCCCGGCCCGCGACAATGACGTCTTGCGGCAACGTGCCGCCCGTTGCCG
CTTCGTGCTGGTGGAAACCAGCCATCCCGGCAATGTCGGTTCGACGGCACGCGCGCTGAAGACCATGGGATTCTGCGAAT
CCGGCAGCTATGTGCTGGTGCGTCCGCGCGAGCCCGGTGCGCAGTCGCATCCCGACGCCGTTGCCATGGCAAGCGGCGCC
GATGACGTGCTCGCCGATGCCCGGGTCGTCGACGATGTCGGCGAGGCGCTGGCCGGGGCTTCGCTCGTCATTGCGCTGAC
GGCGCGTCCGCGCGAGTTCGGCCCGCGCCGCATCGGCCCGCGCGCCGCCGCGCAAGAAGTGGCGGCCTTGCTGGCTGCGC
CGGGGGCGACGGTCGCCTTCGTCTTCGGCAACGAGCGCTACGGTCTGCCCAACGAGGTGGTGGAGCGCTGCCACGTCGTC
ACCCATATCCCCGCCAACCCGGCCTATGCGTCGCTCAACCTGTCGCAGGCGGTGCAGCTGATCGCGTATGAAGTGCGCAT
GGCGCTGCTGGCCGATACCGGCACGGGCGATACCCGCGTCGACGGCGTCGGCTTCGTCGGCGAGCCGGCCACCGCCGAGC
AGATCGACGGCATGTTCGCGCACCTGGAGCGCGGCCTGGTCGAGATCGGTTTTCTCGATCCGGCCCAGCCGAAGAAGCTG
ATGCCGCGCCTGCGGCGCCTGTTTGCCCGCACGCAGCTGGAGGCCGAGGAGGTCAACATCCTGCGCGGCATCGCCAAGCG
CATGCTCGGCCGCCGGGCCGATGCCGCCGCGCCGCCCACCGGCGCCGCCGACGACTGTCGGTGA

Upstream 100 bases:

>100_bases
GCCTGGCGCGGATCGCGCGTCAGGCGAAACAACACAGCGAATTGTGGAAGAACGAACGGCGGGCACGATCCCGCCGCCAA
AAAGAGTGGCCGTATTGTAT

Downstream 100 bases:

>100_bases
TCCCCGCGAAGCCGCTTTCCTGGCGGCTTCTCCCATGCCCGTCAGGCCAAGACCCTTGCCGTAACGCGGCAACGGAATGC
CTTACACTGCCCCCAAGCGC

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MNPASRASETSPARDNDVLRQRAARCRFVLVETSHPGNVGSTARALKTMGFCESGSYVLVRPREPGAQSHPDAVAMASGA
DDVLADARVVDDVGEALAGASLVIALTARPREFGPRRIGPRAAAQEVAALLAAPGATVAFVFGNERYGLPNEVVERCHVV
THIPANPAYASLNLSQAVQLIAYEVRMALLADTGTGDTRVDGVGFVGEPATAEQIDGMFAHLERGLVEIGFLDPAQPKKL
MPRLRRLFARTQLEAEEVNILRGIAKRMLGRRADAAAPPTGAADDCR

Sequences:

>Translated_287_residues
MNPASRASETSPARDNDVLRQRAARCRFVLVETSHPGNVGSTARALKTMGFCESGSYVLVRPREPGAQSHPDAVAMASGA
DDVLADARVVDDVGEALAGASLVIALTARPREFGPRRIGPRAAAQEVAALLAAPGATVAFVFGNERYGLPNEVVERCHVV
THIPANPAYASLNLSQAVQLIAYEVRMALLADTGTGDTRVDGVGFVGEPATAEQIDGMFAHLERGLVEIGFLDPAQPKKL
MPRLRRLFARTQLEAEEVNILRGIAKRMLGRRADAAAPPTGAADDCR
>Mature_287_residues
MNPASRASETSPARDNDVLRQRAARCRFVLVETSHPGNVGSTARALKTMGFCESGSYVLVRPREPGAQSHPDAVAMASGA
DDVLADARVVDDVGEALAGASLVIALTARPREFGPRRIGPRAAAQEVAALLAAPGATVAFVFGNERYGLPNEVVERCHVV
THIPANPAYASLNLSQAVQLIAYEVRMALLADTGTGDTRVDGVGFVGEPATAEQIDGMFAHLERGLVEIGFLDPAQPKKL
MPRLRRLFARTQLEAEEVNILRGIAKRMLGRRADAAAPPTGAADDCR

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=245, Percent_Identity=44.8979591836735, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1790865, Length=164, Percent_Identity=31.7073170731707, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30455; Mature: 30455

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPASRASETSPARDNDVLRQRAARCRFVLVETSHPGNVGSTARALKTMGFCESGSYVLV
CCCCCCCCCCCCCCCHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEE
RPREPGAQSHPDAVAMASGADDVLADARVVDDVGEALAGASLVIALTARPREFGPRRIGP
ECCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHCCCCCCCH
RAAAQEVAALLAAPGATVAFVFGNERYGLPNEVVERCHVVTHIPANPAYASLNLSQAVQL
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHEEECCCCCCEEECCHHHHHHH
IAYEVRMALLADTGTGDTRVDGVGFVGEPATAEQIDGMFAHLERGLVEIGFLDPAQPKKL
HHHHHHHHHHCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCHHHH
MPRLRRLFARTQLEAEEVNILRGIAKRMLGRRADAAAPPTGAADDCR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNPASRASETSPARDNDVLRQRAARCRFVLVETSHPGNVGSTARALKTMGFCESGSYVLV
CCCCCCCCCCCCCCCHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEE
RPREPGAQSHPDAVAMASGADDVLADARVVDDVGEALAGASLVIALTARPREFGPRRIGP
ECCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHCCCCCCCH
RAAAQEVAALLAAPGATVAFVFGNERYGLPNEVVERCHVVTHIPANPAYASLNLSQAVQL
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHEEECCCCCCEEECCHHHHHHH
IAYEVRMALLADTGTGDTRVDGVGFVGEPATAEQIDGMFAHLERGLVEIGFLDPAQPKKL
HHHHHHHHHHCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCHHHH
MPRLRRLFARTQLEAEEVNILRGIAKRMLGRRADAAAPPTGAADDCR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]