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The map label for this gene is phaZ

Identifier: 17545727

GI number: 17545727

Start: 1058015

End: 1059283

Strand: Reverse

Name: phaZ

Synonym: RSc1008

Alternate gene names: NA

Gene position: 1059283-1058015 (Counterclockwise)

Preceding gene: 17545728

Following gene: 17545725

Centisome position: 28.5

GC content: 64.78

Gene sequence:

>1269_bases
ATGCTTTACCAGCTTCACGAATTCCAGCGCGCCATGCTCAGCCCGATGACGGCCTGGGCCCAGGCAACCGCCAAGACCTT
CACCAATCCGCTGAGCCCGCTGTCGCTGCTGCCCGGCGCGCAGCGCTACGCCGCGGGCTACGAACTGCTGTACCGGCTCG
GCAAGGAATACGAGAAGCCGGAATTCAACATCCGCTCGGTCAGGTCGAACGGCCGCGACATTCCCATCGTCGAGCAGACC
ATCATCGAGAAGCCGTTCTGCCGCCTGGTCCGCTTCAAGCGCTATGCCGACGATGCGGAAACCATCCGCATGCTCAAGGA
CGAGCCGGTCGTCCTGGTGTGCGCGCCGCTGTCCGGCCACCACTCGACGCTGCTGCGCGACACGGTGCGCACGCTGCTGC
AGGATCACAAGGTCTATGTGACGGACTGGCTCGACGCACGCATGGTGCCGGTCGGGGCGGGCCCCTTCCACCTGTCCGAC
TACATCGCCTACATCCGCGAATTCATCGCGCACATCGGTGCCGACAAGCTGCATGTGATCTCGGTGTGCCAGCCGACCGT
GCCGGTGCTGGCGGCCATCTCGCTGATGGCATCGGACGGCGAGAAGACGCCGCTGACCATGACGATGATGGGTGGCCCGA
TCGACGCCCGCAAGAGCCCGACCGCCGTCAACTCGCTGGCGACCAACAAGTCGCACGCATGGTTCGAGAACAATGTGATC
TACACCGTGCCGGCCAACTATCCGGGACACGGCCGCCGCGTCTACCCCGGCTTCCTGCAGCACGCCGGCTTCGTGGCGAT
GAATCCCGACCGCCACGTCTCGTCGCATTACGACTACTACCTGAGCCTGGTCGAGGGCGATAAGGAAGACGCCGAAGCCC
ACGTGCGCTTCTACGACGAGTACAACGCCGTGCTGGACATGGCGGCCGAGTATTACCTGGACACCATCCGCGACGTGTTC
CAGGAATTCCACCTGGCCAACGGCACCTGGGTGGTGGATGGCAAGCCGGTGCGCCCGCAGGACATCAAGGGCACCGCGCT
GTTCACCATCGAGGGCGAACTGGACGACATCTCGGGCAGCGGCCAGACCGAGGCGGCGCACGACTTGTGCAGCAGCATCC
CGAAGACGCGCAAGCAGCACCTGACCGCGTCCAACTGCGGCCATTACGGCATCTTCTCGGGTCGGCGCTGGCGCGAGTTC
GTGTATCCGCAGCTGCGCGACTTCATCCGCCGCTACGACAGCAGCACGCGCATCAAGGCGGTCGCCTGA

Upstream 100 bases:

>100_bases
TCTTTATCAGGGTTTCTGCTGCATCGCAGTATGCTGTACCCAGGTGCATAATCAGCAGAATCGTTTGCGTCGTCTTTCGC
CACAACGGACCGGTCCAGCC

Downstream 100 bases:

>100_bases
TCGCCGTGCCTGCACGAAAAAAGCCTCGCTTATCGCGAGGCTTTTTCTTTGGGCTGGCGCCCATCTCAAACCCTGGGATG
CTCGAGATACGCGAGCAGCA

Product: poly[D(-)-3-hydroxybutyrate] depolymerase

Products: NA

Alternate protein names: Intracellular PHB Depolymerase; Polyhydroxyalkanoate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase; PHB Depolymerase; Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Intracellular Poly(3-Hydroxybutyrate) Depolymerase; Intracellular Polyhydroxyalkanoate Depolymerase; PHB De-Polymerase Domain-Containing Protein; Polyhydroxyalkanoate Depolymerase Intracellular Subfamily; Poly(3-Hydroxyalkanoate) Depolymerase; Poly(3-Hydroxybutyrate) Depolymerase; Alpha/Beta Hydrolase Fold; Alpha/Beta Hydrolase Fold Protein; Intracellular PHB Depolymerase Protein; Poly-Beta-Hydroxyalkanoate Depolymerase-Like Protein; PHB De-Polymerase Domain Protein; Intracellular Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Poly[D(-)-3-Hydroxybutyrate](Phb) Depolymerase; Poly-Beta-Hydroxyalkanoate/Butyrate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase Protein; Poly[D(-)-3-Hydroxyalkanoate] Depolymerase; Polyhydroxybutanoate Depolymerase; Poly-Beta-Hydroxybutyrate Depolymerase

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MLYQLHEFQRAMLSPMTAWAQATAKTFTNPLSPLSLLPGAQRYAAGYELLYRLGKEYEKPEFNIRSVRSNGRDIPIVEQT
IIEKPFCRLVRFKRYADDAETIRMLKDEPVVLVCAPLSGHHSTLLRDTVRTLLQDHKVYVTDWLDARMVPVGAGPFHLSD
YIAYIREFIAHIGADKLHVISVCQPTVPVLAAISLMASDGEKTPLTMTMMGGPIDARKSPTAVNSLATNKSHAWFENNVI
YTVPANYPGHGRRVYPGFLQHAGFVAMNPDRHVSSHYDYYLSLVEGDKEDAEAHVRFYDEYNAVLDMAAEYYLDTIRDVF
QEFHLANGTWVVDGKPVRPQDIKGTALFTIEGELDDISGSGQTEAAHDLCSSIPKTRKQHLTASNCGHYGIFSGRRWREF
VYPQLRDFIRRYDSSTRIKAVA

Sequences:

>Translated_422_residues
MLYQLHEFQRAMLSPMTAWAQATAKTFTNPLSPLSLLPGAQRYAAGYELLYRLGKEYEKPEFNIRSVRSNGRDIPIVEQT
IIEKPFCRLVRFKRYADDAETIRMLKDEPVVLVCAPLSGHHSTLLRDTVRTLLQDHKVYVTDWLDARMVPVGAGPFHLSD
YIAYIREFIAHIGADKLHVISVCQPTVPVLAAISLMASDGEKTPLTMTMMGGPIDARKSPTAVNSLATNKSHAWFENNVI
YTVPANYPGHGRRVYPGFLQHAGFVAMNPDRHVSSHYDYYLSLVEGDKEDAEAHVRFYDEYNAVLDMAAEYYLDTIRDVF
QEFHLANGTWVVDGKPVRPQDIKGTALFTIEGELDDISGSGQTEAAHDLCSSIPKTRKQHLTASNCGHYGIFSGRRWREF
VYPQLRDFIRRYDSSTRIKAVA
>Mature_422_residues
MLYQLHEFQRAMLSPMTAWAQATAKTFTNPLSPLSLLPGAQRYAAGYELLYRLGKEYEKPEFNIRSVRSNGRDIPIVEQT
IIEKPFCRLVRFKRYADDAETIRMLKDEPVVLVCAPLSGHHSTLLRDTVRTLLQDHKVYVTDWLDARMVPVGAGPFHLSD
YIAYIREFIAHIGADKLHVISVCQPTVPVLAAISLMASDGEKTPLTMTMMGGPIDARKSPTAVNSLATNKSHAWFENNVI
YTVPANYPGHGRRVYPGFLQHAGFVAMNPDRHVSSHYDYYLSLVEGDKEDAEAHVRFYDEYNAVLDMAAEYYLDTIRDVF
QEFHLANGTWVVDGKPVRPQDIKGTALFTIEGELDDISGSGQTEAAHDLCSSIPKTRKQHLTASNCGHYGIFSGRRWREF
VYPQLRDFIRRYDSSTRIKAVA

Specific function: Unknown

COG id: COG4553

COG function: function code I; Poly-beta-hydroxyalkanoate depolymerase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 47723; Mature: 47723

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYQLHEFQRAMLSPMTAWAQATAKTFTNPLSPLSLLPGAQRYAAGYELLYRLGKEYEKP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCC
EFNIRSVRSNGRDIPIVEQTIIEKPFCRLVRFKRYADDAETIRMLKDEPVVLVCAPLSGH
CCCHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCC
HSTLLRDTVRTLLQDHKVYVTDWLDARMVPVGAGPFHLSDYIAYIREFIAHIGADKLHVI
HHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
SVCQPTVPVLAAISLMASDGEKTPLTMTMMGGPIDARKSPTAVNSLATNKSHAWFENNVI
EECCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHCCCCCCEEECCEE
YTVPANYPGHGRRVYPGFLQHAGFVAMNPDRHVSSHYDYYLSLVEGDKEDAEAHVRFYDE
EEECCCCCCCCCEECCHHHHHCCEEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHH
YNAVLDMAAEYYLDTIRDVFQEFHLANGTWVVDGKPVRPQDIKGTALFTIEGELDDISGS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEEECCCCCCCCC
GQTEAAHDLCSSIPKTRKQHLTASNCGHYGIFSGRRWREFVYPQLRDFIRRYDSSTRIKA
CCCHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
VA
EC
>Mature Secondary Structure
MLYQLHEFQRAMLSPMTAWAQATAKTFTNPLSPLSLLPGAQRYAAGYELLYRLGKEYEKP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCC
EFNIRSVRSNGRDIPIVEQTIIEKPFCRLVRFKRYADDAETIRMLKDEPVVLVCAPLSGH
CCCHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCC
HSTLLRDTVRTLLQDHKVYVTDWLDARMVPVGAGPFHLSDYIAYIREFIAHIGADKLHVI
HHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
SVCQPTVPVLAAISLMASDGEKTPLTMTMMGGPIDARKSPTAVNSLATNKSHAWFENNVI
EECCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHCCCCCCEEECCEE
YTVPANYPGHGRRVYPGFLQHAGFVAMNPDRHVSSHYDYYLSLVEGDKEDAEAHVRFYDE
EEECCCCCCCCCEECCHHHHHCCEEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHH
YNAVLDMAAEYYLDTIRDVFQEFHLANGTWVVDGKPVRPQDIKGTALFTIEGELDDISGS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEEECCCCCCCCC
GQTEAAHDLCSSIPKTRKQHLTASNCGHYGIFSGRRWREFVYPQLRDFIRRYDSSTRIKA
CCCHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
VA
EC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA