| Definition | Ralstonia solanacearum GMI1000, complete genome. |
|---|---|
| Accession | NC_003295 |
| Length | 3,716,413 |
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The map label for this gene is ydfG [H]
Identifier: 17545449
GI number: 17545449
Start: 778153
End: 778941
Strand: Direct
Name: ydfG [H]
Synonym: RSc0730
Alternate gene names: 17545449
Gene position: 778153-778941 (Clockwise)
Preceding gene: 17545428
Following gene: 17545450
Centisome position: 20.94
GC content: 66.79
Gene sequence:
>789_bases TTGCACTTCGCTTATCTCTGGCTCGGAGACACGTCCATGATCGTATTCGTGACCGGCGCAACGGCCGGCTTCGGGGCAGC CGTTGCGCGGCGTTTCGTGCGGGAAGGGCACCGCGTGATCGCAGCGGGCCGCCGCCAAGATCGCCTTGATGCGCTCAAAG CCGAACTGGGCGATGCGCTGCTGCCGTTCCTGCTGGATGTGCAGGATGCCGCCGCCGTCGCCGCCCTGCCGGGGGCCCTG CCGGAGGGCTGGCGGGAGGTCGACGTGCTGGTCAACAATGCCGGCTTGGCGCTCGGCCTGGAGCCGGCGCATCGGGCCAG CCTGTCCGACTGGGACCTGATGATCGGTACCAATGTCACGGGGCTGGTGCATGTGACGCGTGCATTGCTGCCGGGCATGG TGGCGCGCAACCGCGGCCATGTCATCAACCTGGGCTCGATCGCCGGAACGTATCCGTATCCGGGCGGCAACGTGTACGGC GGCACCAAGGCTTTCGTGCGCCAGTTCTCGCTGAACCTGCGCGCCGACCTGACCGGCACCCGCGTGCGCGTGTCGAACGT CGAGCCGGGCCTGTGCTCGGGCACCGAGTTTTCGAACGTGCGCTTCAAGGGCGATGACGCCCGCGTGACCAAGGTCTACG AGGGAACCGAGGCGCTGACGGCCGACGACATCGCCGAGACCATCTACTGGATGGCGGCGCTGCCGGCGCATTTCAATGTC AACGCGATCGAGATCATGCCCGTTTGCCAGTCGTTCTCTGCGACCAGCGTGACGCGCGACATGGCTTGA
Upstream 100 bases:
>100_bases GTTGCCCGCGAGTGTACGACACCCGTATGACCGCCGCAAGGCGCCGCGCGCGGCCTCGCGCACATGGCTGCGCAGCGGGC TGCAATGCTCGGTTAAACTC
Downstream 100 bases:
>100_bases GGCCCGGGCCTGCCATCGCCCGGTTGGTGGATGGCAGGCTGTTTGCTAAGGCTTGGTAAAATCTGGGGATGTCTTCTTTT GACTGGAACCTGCGGGTGTA
Product: oxidoreductase
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 262; Mature: 262
Protein sequence:
>262_residues MHFAYLWLGDTSMIVFVTGATAGFGAAVARRFVREGHRVIAAGRRQDRLDALKAELGDALLPFLLDVQDAAAVAALPGAL PEGWREVDVLVNNAGLALGLEPAHRASLSDWDLMIGTNVTGLVHVTRALLPGMVARNRGHVINLGSIAGTYPYPGGNVYG GTKAFVRQFSLNLRADLTGTRVRVSNVEPGLCSGTEFSNVRFKGDDARVTKVYEGTEALTADDIAETIYWMAALPAHFNV NAIEIMPVCQSFSATSVTRDMA
Sequences:
>Translated_262_residues MHFAYLWLGDTSMIVFVTGATAGFGAAVARRFVREGHRVIAAGRRQDRLDALKAELGDALLPFLLDVQDAAAVAALPGAL PEGWREVDVLVNNAGLALGLEPAHRASLSDWDLMIGTNVTGLVHVTRALLPGMVARNRGHVINLGSIAGTYPYPGGNVYG GTKAFVRQFSLNLRADLTGTRVRVSNVEPGLCSGTEFSNVRFKGDDARVTKVYEGTEALTADDIAETIYWMAALPAHFNV NAIEIMPVCQSFSATSVTRDMA >Mature_262_residues MHFAYLWLGDTSMIVFVTGATAGFGAAVARRFVREGHRVIAAGRRQDRLDALKAELGDALLPFLLDVQDAAAVAALPGAL PEGWREVDVLVNNAGLALGLEPAHRASLSDWDLMIGTNVTGLVHVTRALLPGMVARNRGHVINLGSIAGTYPYPGGNVYG GTKAFVRQFSLNLRADLTGTRVRVSNVEPGLCSGTEFSNVRFKGDDARVTKVYEGTEALTADDIAETIYWMAALPAHFNV NAIEIMPVCQSFSATSVTRDMA
Specific function: Catalyzes the NADP-dependent oxidation of L-allo- threonine and L-serine [H]
COG id: COG4221
COG function: function code R; Short-chain alcohol dehydrogenase of unknown specificity
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]
Homologues:
Organism=Homo sapiens, GI109715829, Length=248, Percent_Identity=30.6451612903226, Blast_Score=114, Evalue=1e-25, Organism=Homo sapiens, GI150247226, Length=190, Percent_Identity=35.2631578947368, Blast_Score=96, Evalue=5e-20, Organism=Homo sapiens, GI40548397, Length=211, Percent_Identity=35.5450236966825, Blast_Score=94, Evalue=1e-19, Organism=Homo sapiens, GI214010158, Length=211, Percent_Identity=35.5450236966825, Blast_Score=94, Evalue=1e-19, Organism=Homo sapiens, GI214010156, Length=211, Percent_Identity=35.5450236966825, Blast_Score=94, Evalue=1e-19, Organism=Homo sapiens, GI22758144, Length=211, Percent_Identity=35.5450236966825, Blast_Score=94, Evalue=1e-19, Organism=Homo sapiens, GI20149619, Length=191, Percent_Identity=30.3664921465969, Blast_Score=91, Evalue=8e-19, Organism=Homo sapiens, GI22507405, Length=238, Percent_Identity=29.4117647058824, Blast_Score=87, Evalue=1e-17, Organism=Homo sapiens, GI7705855, Length=194, Percent_Identity=29.3814432989691, Blast_Score=86, Evalue=4e-17, Organism=Homo sapiens, GI157739938, Length=184, Percent_Identity=29.8913043478261, Blast_Score=83, Evalue=2e-16, Organism=Homo sapiens, GI19743808, Length=178, Percent_Identity=34.2696629213483, Blast_Score=82, Evalue=4e-16, Organism=Homo sapiens, GI7706318, Length=188, Percent_Identity=30.8510638297872, Blast_Score=80, Evalue=2e-15, Organism=Homo sapiens, GI7657478, Length=183, Percent_Identity=32.2404371584699, Blast_Score=75, Evalue=8e-14, Organism=Homo sapiens, GI4557649, Length=178, Percent_Identity=26.9662921348315, Blast_Score=73, Evalue=3e-13, Organism=Homo sapiens, GI5031737, Length=197, Percent_Identity=31.9796954314721, Blast_Score=72, Evalue=4e-13, Organism=Homo sapiens, GI32483357, Length=193, Percent_Identity=29.0155440414508, Blast_Score=70, Evalue=2e-12, Organism=Homo sapiens, GI33667109, Length=190, Percent_Identity=32.1052631578947, Blast_Score=69, Evalue=5e-12, Organism=Homo sapiens, GI44680136, Length=190, Percent_Identity=30.5263157894737, Blast_Score=65, Evalue=4e-11, Organism=Homo sapiens, GI44680133, Length=190, Percent_Identity=30.5263157894737, Blast_Score=65, Evalue=4e-11, Organism=Homo sapiens, GI17738292, Length=190, Percent_Identity=30.5263157894737, Blast_Score=65, Evalue=4e-11, Organism=Homo sapiens, GI50726952, Length=191, Percent_Identity=32.4607329842932, Blast_Score=65, Evalue=5e-11, Organism=Homo sapiens, GI40254992, Length=179, Percent_Identity=29.608938547486, Blast_Score=65, Evalue=9e-11, Organism=Escherichia coli, GI1787820, Length=248, Percent_Identity=61.6935483870968, Blast_Score=336, Evalue=1e-93, Organism=Escherichia coli, GI1787335, Length=195, Percent_Identity=29.2307692307692, Blast_Score=82, Evalue=5e-17, Organism=Escherichia coli, GI87082100, Length=184, Percent_Identity=32.6086956521739, Blast_Score=80, Evalue=1e-16, Organism=Escherichia coli, GI1786812, Length=243, Percent_Identity=27.1604938271605, Blast_Score=70, Evalue=1e-13, Organism=Caenorhabditis elegans, GI71994604, Length=240, Percent_Identity=27.9166666666667, Blast_Score=86, Evalue=3e-17, Organism=Caenorhabditis elegans, GI71994600, Length=242, Percent_Identity=27.2727272727273, Blast_Score=82, Evalue=2e-16, Organism=Caenorhabditis elegans, GI17538480, Length=191, Percent_Identity=28.2722513089005, Blast_Score=79, Evalue=3e-15, Organism=Caenorhabditis elegans, GI17562910, Length=215, Percent_Identity=28.3720930232558, Blast_Score=78, Evalue=5e-15, Organism=Caenorhabditis elegans, GI17559104, Length=237, Percent_Identity=25.3164556962025, Blast_Score=74, Evalue=5e-14, Organism=Caenorhabditis elegans, GI17562908, Length=187, Percent_Identity=29.4117647058824, Blast_Score=73, Evalue=1e-13, Organism=Caenorhabditis elegans, GI17544670, Length=247, Percent_Identity=25.9109311740891, Blast_Score=72, Evalue=3e-13, Organism=Caenorhabditis elegans, GI17531453, Length=187, Percent_Identity=28.3422459893048, Blast_Score=71, Evalue=5e-13, Organism=Caenorhabditis elegans, GI17560676, Length=208, Percent_Identity=25, Blast_Score=71, Evalue=7e-13, Organism=Caenorhabditis elegans, GI17562906, Length=187, Percent_Identity=28.8770053475936, Blast_Score=70, Evalue=1e-12, Organism=Caenorhabditis elegans, GI17560150, Length=184, Percent_Identity=27.7173913043478, Blast_Score=70, Evalue=1e-12, Organism=Caenorhabditis elegans, GI17560332, Length=202, Percent_Identity=28.7128712871287, Blast_Score=69, Evalue=2e-12, Organism=Caenorhabditis elegans, GI17562990, Length=186, Percent_Identity=27.4193548387097, Blast_Score=67, Evalue=7e-12, Organism=Caenorhabditis elegans, GI193204405, Length=193, Percent_Identity=29.5336787564767, Blast_Score=67, Evalue=9e-12, Organism=Caenorhabditis elegans, GI17562904, Length=211, Percent_Identity=27.0142180094787, Blast_Score=66, Evalue=2e-11, Organism=Caenorhabditis elegans, GI17538486, Length=198, Percent_Identity=26.2626262626263, Blast_Score=66, Evalue=2e-11, Organism=Caenorhabditis elegans, GI17565030, Length=227, Percent_Identity=27.3127753303965, Blast_Score=65, Evalue=3e-11, Organism=Caenorhabditis elegans, GI25152911, Length=197, Percent_Identity=28.4263959390863, Blast_Score=65, Evalue=3e-11, Organism=Saccharomyces cerevisiae, GI6323882, Length=246, Percent_Identity=35.7723577235772, Blast_Score=154, Evalue=1e-38, Organism=Saccharomyces cerevisiae, GI6322067, Length=178, Percent_Identity=30.3370786516854, Blast_Score=84, Evalue=2e-17, Organism=Saccharomyces cerevisiae, GI6322227, Length=199, Percent_Identity=25.1256281407035, Blast_Score=68, Evalue=1e-12, Organism=Drosophila melanogaster, GI116007236, Length=253, Percent_Identity=32.0158102766798, Blast_Score=113, Evalue=1e-25, Organism=Drosophila melanogaster, GI21358495, Length=229, Percent_Identity=32.7510917030568, Blast_Score=108, Evalue=5e-24, Organism=Drosophila melanogaster, GI23397609, Length=240, Percent_Identity=30.4166666666667, Blast_Score=95, Evalue=5e-20, Organism=Drosophila melanogaster, GI24640442, Length=195, Percent_Identity=30.2564102564103, Blast_Score=91, Evalue=1e-18, Organism=Drosophila melanogaster, GI116007238, Length=188, Percent_Identity=35.1063829787234, Blast_Score=88, Evalue=5e-18, Organism=Drosophila melanogaster, GI28571526, Length=182, Percent_Identity=32.4175824175824, Blast_Score=88, Evalue=7e-18, Organism=Drosophila melanogaster, GI24584748, Length=197, Percent_Identity=30.4568527918782, Blast_Score=85, Evalue=4e-17, Organism=Drosophila melanogaster, GI161076933, Length=197, Percent_Identity=30.4568527918782, Blast_Score=85, Evalue=4e-17, Organism=Drosophila melanogaster, GI116007234, Length=253, Percent_Identity=32.0158102766798, Blast_Score=85, Evalue=5e-17, Organism=Drosophila melanogaster, GI21357041, Length=182, Percent_Identity=30.2197802197802, Blast_Score=84, Evalue=1e-16, Organism=Drosophila melanogaster, GI281364494, Length=251, Percent_Identity=29.0836653386454, Blast_Score=83, Evalue=2e-16, Organism=Drosophila melanogaster, GI24643142, Length=187, Percent_Identity=30.4812834224599, Blast_Score=83, Evalue=2e-16, Organism=Drosophila melanogaster, GI24644339, Length=239, Percent_Identity=28.4518828451883, Blast_Score=82, Evalue=3e-16, Organism=Drosophila melanogaster, GI24641232, Length=257, Percent_Identity=28.0155642023346, Blast_Score=81, Evalue=6e-16, Organism=Drosophila melanogaster, GI24663911, Length=267, Percent_Identity=28.0898876404494, Blast_Score=80, Evalue=1e-15, Organism=Drosophila melanogaster, GI28571387, Length=254, Percent_Identity=27.5590551181102, Blast_Score=78, Evalue=5e-15, Organism=Drosophila melanogaster, GI24641388, Length=259, Percent_Identity=27.027027027027, Blast_Score=75, Evalue=5e-14, Organism=Drosophila melanogaster, GI24648693, Length=258, Percent_Identity=26.7441860465116, Blast_Score=74, Evalue=1e-13, Organism=Drosophila melanogaster, GI21355889, Length=258, Percent_Identity=26.7441860465116, Blast_Score=74, Evalue=1e-13, Organism=Drosophila melanogaster, GI28574402, Length=192, Percent_Identity=28.125, Blast_Score=72, Evalue=3e-13, Organism=Drosophila melanogaster, GI24584744, Length=192, Percent_Identity=28.125, Blast_Score=72, Evalue=3e-13, Organism=Drosophila melanogaster, GI17737361, Length=192, Percent_Identity=29.1666666666667, Blast_Score=68, Evalue=5e-12, Organism=Drosophila melanogaster, GI24580925, Length=209, Percent_Identity=26.7942583732057, Blast_Score=67, Evalue=9e-12, Organism=Drosophila melanogaster, GI116007232, Length=184, Percent_Identity=33.695652173913, Blast_Score=65, Evalue=6e-11,
Paralogues:
None
Copy number: 1300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002198 - InterPro: IPR002347 - InterPro: IPR016040 - InterPro: IPR020904 [H]
Pfam domain/function: PF00106 adh_short [H]
EC number: 1.-.-.- [C]
Molecular weight: Translated: 28044; Mature: 28044
Theoretical pI: Translated: 6.51; Mature: 6.51
Prosite motif: PS00061 ADH_SHORT
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 2.7 %Met (Translated Protein) 3.4 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 2.7 %Met (Mature Protein) 3.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MHFAYLWLGDTSMIVFVTGATAGFGAAVARRFVREGHRVIAAGRRQDRLDALKAELGDAL CEEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHH LPFLLDVQDAAAVAALPGALPEGWREVDVLVNNAGLALGLEPAHRASLSDWDLMIGTNVT HHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCEEEECCHHHHCCCCCCEEEECCCCH GLVHVTRALLPGMVARNRGHVINLGSIAGTYPYPGGNVYGGTKAFVRQFSLNLRADLTGT HHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCEEECCCCC RVRVSNVEPGLCSGTEFSNVRFKGDDARVTKVYEGTEALTADDIAETIYWMAALPAHFNV EEEECCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCC NAIEIMPVCQSFSATSVTRDMA CEEEEEEHHCCCCCHHHHHCCC >Mature Secondary Structure MHFAYLWLGDTSMIVFVTGATAGFGAAVARRFVREGHRVIAAGRRQDRLDALKAELGDAL CEEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHH LPFLLDVQDAAAVAALPGALPEGWREVDVLVNNAGLALGLEPAHRASLSDWDLMIGTNVT HHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCEEEECCHHHHCCCCCCEEEECCCCH GLVHVTRALLPGMVARNRGHVINLGSIAGTYPYPGGNVYGGTKAFVRQFSLNLRADLTGT HHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCEEECCCCC RVRVSNVEPGLCSGTEFSNVRFKGDDARVTKVYEGTEALTADDIAETIYWMAALPAHFNV EEEECCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCC NAIEIMPVCQSFSATSVTRDMA CEEEEEEHHCCCCCHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 11677608; 12644504 [H]