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The map label for this gene is rsuA [H]

Identifier: 17545393

GI number: 17545393

Start: 723578

End: 724336

Strand: Direct

Name: rsuA [H]

Synonym: RSc0674

Alternate gene names: 17545393

Gene position: 723578-724336 (Clockwise)

Preceding gene: 17545392

Following gene: 17545394

Centisome position: 19.47

GC content: 69.17

Gene sequence:

>759_bases
ATGACCGCTTCTTCCGCCCGCCTGCCGCTCTACCGCATCTTGCAATCCCAGGGGTTCGGCACGCGCCGCTACTGCAAGGA
CCTGGTGTTCGCCGGCCTGGTCCACGTCAACGGCGTCGAGGCCGAGGACCCCGATGCCCAGGTCGACACCGCGGGCCTCG
TGCTGGACGTCGACGGCGAACGCTGGGCCTACCACGCGCGTGCCTACCTGATGCTCCACAAGCCCACGGGTTTCGAGTGC
TCGCAGAAGCCGCGCCACCATCCCAGCGTCTACACGCTGCTGCCGGTGCCTTTGCGCGAACGCGGCGTGCAGTGCGTCGG
CCGTCTCGACCAGGACACCACGGGCCTGCTGCTGCTGACCGACGATGGCCAGTTCGTCCACACGCTGACCAGCCCTCGCC
ACCAGGTACCGAAGGTGTACGAGATCACCACCGCCGAGCCCGTGACGGACGCGCAGGTCACCCAACTGTGCCAGGGCGTG
CTGCTGGAAGACGAAGATGTGCCGGTGGCGGCCGCCTGGGCCGAGCGGGCGGATGCCTGCCACCTGCGCATGGCGCTCAC
GCAGGGAAAATACCATCAGGTCAAGCGGATGGTGGCGGCCGCCGGCAACCATGTCGCCGCGCTGCATCGCAGTGCCATCG
GCGGCCTGACGCTGGGTGCCGACTTGGCGCCGGGCCAATGGCGCTGGCTGGAGAGCGACGACCTGGTGGCCCTGGATACC
ACGCCGGTTCATCCCGCCGGGGCGGCCATGGGTGCGTGA

Upstream 100 bases:

>100_bases
AGGGCTTGGCGAGCGATGGACGCGGCATGACCGAACGCCTGGTGGCCGGCCGAGCGTATTGCACAATCCGCGCCGCATCC
TATCTCCATCCGTTGTTGCC

Downstream 100 bases:

>100_bases
GCCTTCACAAAAAATTACATCCGGGGGCCGACTTCCACCAAATGAAAAGGCCACCCGCCGGGGGCTTTTGGTAAACTAGC
CTCATGGCCTCGCCGGACGC

Product: ribosomal small subunit pseudouridine synthase A protein

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MTASSARLPLYRILQSQGFGTRRYCKDLVFAGLVHVNGVEAEDPDAQVDTAGLVLDVDGERWAYHARAYLMLHKPTGFEC
SQKPRHHPSVYTLLPVPLRERGVQCVGRLDQDTTGLLLLTDDGQFVHTLTSPRHQVPKVYEITTAEPVTDAQVTQLCQGV
LLEDEDVPVAAAWAERADACHLRMALTQGKYHQVKRMVAAAGNHVAALHRSAIGGLTLGADLAPGQWRWLESDDLVALDT
TPVHPAGAAMGA

Sequences:

>Translated_252_residues
MTASSARLPLYRILQSQGFGTRRYCKDLVFAGLVHVNGVEAEDPDAQVDTAGLVLDVDGERWAYHARAYLMLHKPTGFEC
SQKPRHHPSVYTLLPVPLRERGVQCVGRLDQDTTGLLLLTDDGQFVHTLTSPRHQVPKVYEITTAEPVTDAQVTQLCQGV
LLEDEDVPVAAAWAERADACHLRMALTQGKYHQVKRMVAAAGNHVAALHRSAIGGLTLGADLAPGQWRWLESDDLVALDT
TPVHPAGAAMGA
>Mature_251_residues
TASSARLPLYRILQSQGFGTRRYCKDLVFAGLVHVNGVEAEDPDAQVDTAGLVLDVDGERWAYHARAYLMLHKPTGFECS
QKPRHHPSVYTLLPVPLRERGVQCVGRLDQDTTGLLLLTDDGQFVHTLTSPRHQVPKVYEITTAEPVTDAQVTQLCQGVL
LEDEDVPVAAAWAERADACHLRMALTQGKYHQVKRMVAAAGNHVAALHRSAIGGLTLGADLAPGQWRWLESDDLVALDTT
PVHPAGAAMGA

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=174, Percent_Identity=45.4022988505747, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1787524, Length=226, Percent_Identity=31.858407079646, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790453, Length=238, Percent_Identity=27.7310924369748, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081838, Length=144, Percent_Identity=34.0277777777778, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 27427; Mature: 27296

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASSARLPLYRILQSQGFGTRRYCKDLVFAGLVHVNGVEAEDPDAQVDTAGLVLDVDGE
CCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCEECCEEEEEECCC
RWAYHARAYLMLHKPTGFECSQKPRHHPSVYTLLPVPLRERGVQCVGRLDQDTTGLLLLT
CEEEEEEEEEEEECCCCCCCCCCCCCCCCEEEEECCCHHHCCHHHHHCCCCCCCEEEEEE
DDGQFVHTLTSPRHQVPKVYEITTAEPVTDAQVTQLCQGVLLEDEDVPVAAAWAERADAC
CCCCEEEEECCCCHHCCEEEEEECCCCCCHHHHHHHHHCCEECCCCCCEEEHHHHCCCCE
HLRMALTQGKYHQVKRMVAAAGNHVAALHRSAIGGLTLGADLAPGQWRWLESDDLVALDT
EEEEEHHCCHHHHHHHHHHHCCCHHHHHHHHHHCCEEECCCCCCCCEEEECCCCEEEEEC
TPVHPAGAAMGA
CCCCCCCCCCCC
>Mature Secondary Structure 
TASSARLPLYRILQSQGFGTRRYCKDLVFAGLVHVNGVEAEDPDAQVDTAGLVLDVDGE
CCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCEECCEEEEEECCC
RWAYHARAYLMLHKPTGFECSQKPRHHPSVYTLLPVPLRERGVQCVGRLDQDTTGLLLLT
CEEEEEEEEEEEECCCCCCCCCCCCCCCCEEEEECCCHHHCCHHHHHCCCCCCCEEEEEE
DDGQFVHTLTSPRHQVPKVYEITTAEPVTDAQVTQLCQGVLLEDEDVPVAAAWAERADAC
CCCCEEEEECCCCHHCCEEEEEECCCCCCHHHHHHHHHCCEECCCCCCEEEHHHHCCCCE
HLRMALTQGKYHQVKRMVAAAGNHVAALHRSAIGGLTLGADLAPGQWRWLESDDLVALDT
EEEEEHHCCHHHHHHHHHHHCCCHHHHHHHHHHCCEEECCCCCCCCEEEECCCCEEEEEC
TPVHPAGAAMGA
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]