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The map label for this gene is recA

Identifier: 17545270

GI number: 17545270

Start: 596177

End: 597235

Strand: Direct

Name: recA

Synonym: RSc0551

Alternate gene names: 17545270

Gene position: 596177-597235 (Clockwise)

Preceding gene: 17545265

Following gene: 17545271

Centisome position: 16.04

GC content: 64.78

Gene sequence:

>1059_bases
ATGGAAGACGGCAAGAAGGCAGCCTCGATGAGCGCAGAAAAGCAGAAGGCGCTGGCTGCCGCGCTCGCGCAGATCGAAAA
GCAGTTCGGCAAGGGCTCGATCATGAAGATGGGCGACGCCGAGGTGGAGCCGGTCCAGGTTGTGTCCACCGGCTCGCTGG
GTCTGGACGTCGCGCTGGGCGTCGGCGGCCTGCCGCGCGGTCGCGTGGTCGAGATCTACGGCCCCGAATCGTCCGGCAAG
ACCACGCTGACGCTGCAGGTGGTCGCCGAGATGCAGAAGCTGGGCGGCACCTGCGCCTTCATCGACGCCGAGCATGCGCT
GGACGTCACCTATGCCGACAAGCTCGGCGTGAAGGTGCCGGACCTGCTGATCTCCCAACCGGACACCGGTGAGCAGGCGC
TGGAAATCGCTGACGCGCTGGTGCGCTCGGGCTCGGTCGACCTGATCGTCATCGACTCGGTGGCCGCGCTGGTGCCCAAG
GCCGAAATCGAAGGCGAGATGGGCGACGCGCTGCCCGGTCTGCAGGCCCGCCTGATGAGCCAGGCGCTGCGCAAGCTGAC
CGGCACCATCAAGCGCACCAACTGCCTGGTGATCTTCATCAACCAGATCCGCATGAAGATCGGCGTGATGTTCGGCTCGC
CGGAAACCACCACGGGCGGCAATGCGCTCAAGTTTTACGCTTCGGTGCGTCTGGACATCCGCCGCATCGGCTCGATCAAG
AAGGGCGATGAGGTGGTCGGCAACGAGACCAAGGTCAAGGTCGTCAAGAACAAGGTGGCGCCGCCGTTCCGCGAGGCGAT
CTTCGACATCCTCTACGGTGCGGGCGTGTCGCGCGAAGGCGAGATCATCGATCTGGGCGTGGAAGCCAAGGTGGTCGAGA
AATCCGGCGCCTGGTACAGCTACAACGGCGAGCGCATCGGTCAGGGCCGTGACAACTGCCGCGAATTCCTGCGCGAAAAC
GCCGAACTGGCCCGCGAGATCGAAAACAAGGTCCGCGAACACCTGGGCGTGACGCCGATGGGCGCCGTCACGCTGGCCGA
GGAAGTCGAGGAAGATTGA

Upstream 100 bases:

>100_bases
CGGACACCGGACGGTGACGCGCGGCCCGCCATTGCGGACGACCGCTGGAATCCCAGGCCCTTGCGGCCAGTTTATACACC
AATTTGCACGAAGGACGACC

Downstream 100 bases:

>100_bases
TTGGGTTGGCTGTCGGCGCAGATCGACAGCCGTATCGAGATTGACGCGCCTGCCGTTTCGCGACGGTAGGCGTTTTTCTT
TGGAGCGTGTCCGGATGGCG

Product: recombinase A

Products: NA

Alternate protein names: Recombinase A

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MEDGKKAASMSAEKQKALAAALAQIEKQFGKGSIMKMGDAEVEPVQVVSTGSLGLDVALGVGGLPRGRVVEIYGPESSGK
TTLTLQVVAEMQKLGGTCAFIDAEHALDVTYADKLGVKVPDLLISQPDTGEQALEIADALVRSGSVDLIVIDSVAALVPK
AEIEGEMGDALPGLQARLMSQALRKLTGTIKRTNCLVIFINQIRMKIGVMFGSPETTTGGNALKFYASVRLDIRRIGSIK
KGDEVVGNETKVKVVKNKVAPPFREAIFDILYGAGVSREGEIIDLGVEAKVVEKSGAWYSYNGERIGQGRDNCREFLREN
AELAREIENKVREHLGVTPMGAVTLAEEVEED

Sequences:

>Translated_352_residues
MEDGKKAASMSAEKQKALAAALAQIEKQFGKGSIMKMGDAEVEPVQVVSTGSLGLDVALGVGGLPRGRVVEIYGPESSGK
TTLTLQVVAEMQKLGGTCAFIDAEHALDVTYADKLGVKVPDLLISQPDTGEQALEIADALVRSGSVDLIVIDSVAALVPK
AEIEGEMGDALPGLQARLMSQALRKLTGTIKRTNCLVIFINQIRMKIGVMFGSPETTTGGNALKFYASVRLDIRRIGSIK
KGDEVVGNETKVKVVKNKVAPPFREAIFDILYGAGVSREGEIIDLGVEAKVVEKSGAWYSYNGERIGQGRDNCREFLREN
AELAREIENKVREHLGVTPMGAVTLAEEVEED
>Mature_352_residues
MEDGKKAASMSAEKQKALAAALAQIEKQFGKGSIMKMGDAEVEPVQVVSTGSLGLDVALGVGGLPRGRVVEIYGPESSGK
TTLTLQVVAEMQKLGGTCAFIDAEHALDVTYADKLGVKVPDLLISQPDTGEQALEIADALVRSGSVDLIVIDSVAALVPK
AEIEGEMGDALPGLQARLMSQALRKLTGTIKRTNCLVIFINQIRMKIGVMFGSPETTTGGNALKFYASVRLDIRRIGSIK
KGDEVVGNETKVKVVKNKVAPPFREAIFDILYGAGVSREGEIIDLGVEAKVVEKSGAWYSYNGERIGQGRDNCREFLREN
AELAREIENKVREHLGVTPMGAVTLAEEVEED

Specific function: Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family

Homologues:

Organism=Escherichia coli, GI1789051, Length=326, Percent_Identity=70.8588957055215, Blast_Score=478, Evalue=1e-136,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RECA_RALSO (Q8Y1Y6)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_518672.1
- PDB:   1V17
- PDBsum:   1V17
- ProteinModelPortal:   Q8Y1Y6
- SMR:   Q8Y1Y6
- GeneID:   1219356
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc0551
- NMPDR:   fig|267608.1.peg.551
- HOGENOM:   HBG339889
- OMA:   IEINENQ
- ProtClustDB:   PRK09354
- BioCyc:   RSOL267608:RSC0551-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00268
- InterPro:   IPR003593
- InterPro:   IPR013765
- InterPro:   IPR020584
- InterPro:   IPR020588
- InterPro:   IPR020587
- PANTHER:   PTHR22942:SF1
- PRINTS:   PR00142
- SMART:   SM00382
- TIGRFAMs:   TIGR02012

Pfam domain/function: PF00154 RecA

EC number: NA

Molecular weight: Translated: 37659; Mature: 37659

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00321 RECA_1; PS50162 RECA_2; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDGKKAASMSAEKQKALAAALAQIEKQFGKGSIMKMGDAEVEPVQVVSTGSLGLDVALG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCCCCEEEEEC
VGGLPRGRVVEIYGPESSGKTTLTLQVVAEMQKLGGTCAFIDAEHALDVTYADKLGVKVP
CCCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHHCCEEEEEECCHHEEEEEHHHCCCCCC
DLLISQPDTGEQALEIADALVRSGSVDLIVIDSVAALVPKAEIEGEMGDALPGLQARLMS
HHHCCCCCCCHHHHHHHHHHHHCCCCCEEEECCHHHHCCCHHCCCCCCCCCCHHHHHHHH
QALRKLTGTIKRTNCLVIFINQIRMKIGVMFGSPETTTGGNALKFYASVRLDIRRIGSIK
HHHHHHHHHHHHCCEEEEEEEHHEEEEEEEECCCCCCCCCCEEEEEEEHHHHHHHHCCCC
KGDEVVGNETKVKVVKNKVAPPFREAIFDILYGAGVSREGEIIDLGVEAKVVEKSGAWYS
CCCHHCCCCCEEEEEHHCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCEEE
YNGERIGQGRDNCREFLRENAELAREIENKVREHLGVTPMGAVTLAEEVEED
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MEDGKKAASMSAEKQKALAAALAQIEKQFGKGSIMKMGDAEVEPVQVVSTGSLGLDVALG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCCCCEEEEEC
VGGLPRGRVVEIYGPESSGKTTLTLQVVAEMQKLGGTCAFIDAEHALDVTYADKLGVKVP
CCCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHHCCEEEEEECCHHEEEEEHHHCCCCCC
DLLISQPDTGEQALEIADALVRSGSVDLIVIDSVAALVPKAEIEGEMGDALPGLQARLMS
HHHCCCCCCCHHHHHHHHHHHHCCCCCEEEECCHHHHCCCHHCCCCCCCCCCHHHHHHHH
QALRKLTGTIKRTNCLVIFINQIRMKIGVMFGSPETTTGGNALKFYASVRLDIRRIGSIK
HHHHHHHHHHHHCCEEEEEEEHHEEEEEEEECCCCCCCCCCEEEEEEEHHHHHHHHCCCC
KGDEVVGNETKVKVVKNKVAPPFREAIFDILYGAGVSREGEIIDLGVEAKVVEKSGAWYS
CCCHHCCCCCEEEEEHHCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCEEE
YNGERIGQGRDNCREFLRENAELAREIENKVREHLGVTPMGAVTLAEEVEED
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852