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The map label for this gene is proP [H]

Identifier: 17545266

GI number: 17545266

Start: 591452

End: 593155

Strand: Reverse

Name: proP [H]

Synonym: RSc0547

Alternate gene names: 17545266

Gene position: 593155-591452 (Counterclockwise)

Preceding gene: 17545267

Following gene: 17545264

Centisome position: 15.96

GC content: 64.85

Gene sequence:

>1704_bases
ATGGCAACCGTGCAAAGTGCACCGAACGCGCCCGTCGGGCGCATGCAGCCCTCGCCCATGACCCGGGAGGAGAAGAAGGT
CATCTTCGCCTCCTCGCTGGGCACGGTGTTCGAGTGGTACGACTTCTATCTCTATGGGTCCCTGGCGGCCATCATCGCCA
AGCAGTTCTTTTCCGGGCTGGACCCGACGGCGGCGTTCATCTTTGCGCTGCTGGCATTCGCGGCGGGCTTCCTGGTGCGG
CCGTTCGGCGCGCTGGTGTTCGGCCGGCTGGGCGACATGATCGGGCGCAAGTACACCTTCCTGATCACCATCCTGATCAT
GGGCACGTCGACCTTCATCGTCGGCCTGCTGCCCTCCTACACCACGATCGGGGCGGCCGCACCGGTCATCCTCATCATGC
TGCGCCTGCTGCAGGGCCTGGCGCTGGGCGGCGAGTACGGCGGTGCCGCCACCTACGTCGCCGAGCACGCGCCGCACGGG
CGGCGGGGCAATTACACGTCGTGGATCCAGACCACCGCCACGCTGGGCCTGTTCCTGTCGCTGATCGTGATCCTGGTGGT
GCGCGAGCTGACCGGCGCATCGTTCGAGGACTGGGGCTGGCGCATCCCGTTCCTGGTGTCGATCGTGCTGCTGGCCACGT
CGGTGTACATCCGCCTGTCGATGAGCGAGTCGCCGGCGTTCCAGAAGATGAAGGCCGAGGGCAAGACCTCCAAGGCCCCG
CTGACGGAATCGTTCGGCCAGTGGCGCAACCTGAAGATCGTGATCCTGGCGCTGGTGGGCCTGACCGCCGGCCAGGCCGT
GGTCTGGTACACGGGCCAGTTCTATGCGCTGTTCTTCCTGACGCAGGTGCTGAAGGTGGATGCGTTCACGGCCAACGTGC
TGATTGCCGTGGCGCTGGCCATCGGCACGCCGTTCTTCGTGTTCTTCGGCTCGCTGTCGGACCGCATCGGCCGCAAGGGG
ATCATCATGGCCGGTTGCCTGCTGGCGGTGCTGACCTACTTCCCGCTGTTCAAGGCGCTCACCCACTATGCCAATCCGGC
GCTGGAGCGTGCCCAGCAGACCGCGCAGATCACCATCAAGGCCGATCCGAAGGAATGCTCGTTCCAGGGCAGCCCGATCG
CGCGGGAAGTCGATTTCCGCTCGTCGTGCGACATCGCCAAGCGCACGCTCGCGCAATCGTCGGCCAGCTATGAATCGGAA
GACGCGCCGGCCGGCACCATCGCCTCGGTGACGATCGCCGGCAGGGAAATCGCCTCGCTCAACGCCAGCCGCACGGCCGA
CGGCCAGAACTTCGATGCCGACAGCAAGAAGAAGATCACCGACTTCAAGAAATCGGTGAGCGACGCGCTCAAGGCGGCGA
ACTACCCGAGCAAGGCCGACCCGGCGCAGATGAACACGGTGATGGTGCTGGTGATCCTGGTGATCCTGGTGATCTACGTG
ACCATGGTCTATGGCCCGATCGCGGCAATGCTGGTGGAGCTGTTCCCGACGCGCATCCGCTATACGTCGATGTCGCTGCC
GTACCACATCGGCAACGGCTGGTTCGGCGGCCTGCTGCCGACCATCTCGTTCGCGCTGGTGGCGCAGCACGGCAACATCT
ACTACGGGCTGTGGTACCCGATCTGGATTGCCGCCATCACCTTCGTGATCGGGGCCCTGTTCGTGCGGGAGACCAAGGAC
GTCGACATCTATCGCCACGACTGA

Upstream 100 bases:

>100_bases
CAGTAAGTTTCGGCTCCCACAATCGTTCGCAATTCCGCGGGCGCTGCGTTGCGTTGTCATGTCCCATGCCCAGCGGGAAG
ACTCACGAACAGGAGACAAC

Downstream 100 bases:

>100_bases
CGATAGGTGCTTGACACCCCATCGGACAACGGTATAATCGCGGTTTTCGGCGAATTAGCTCAGTCGGTTAGAGCGACGGA
ATCATAATCCGCAGGTCCGG

Product: sugar transporter transmembrane protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MATVQSAPNAPVGRMQPSPMTREEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFSGLDPTAAFIFALLAFAAGFLVR
PFGALVFGRLGDMIGRKYTFLITILIMGTSTFIVGLLPSYTTIGAAAPVILIMLRLLQGLALGGEYGGAATYVAEHAPHG
RRGNYTSWIQTTATLGLFLSLIVILVVRELTGASFEDWGWRIPFLVSIVLLATSVYIRLSMSESPAFQKMKAEGKTSKAP
LTESFGQWRNLKIVILALVGLTAGQAVVWYTGQFYALFFLTQVLKVDAFTANVLIAVALAIGTPFFVFFGSLSDRIGRKG
IIMAGCLLAVLTYFPLFKALTHYANPALERAQQTAQITIKADPKECSFQGSPIAREVDFRSSCDIAKRTLAQSSASYESE
DAPAGTIASVTIAGREIASLNASRTADGQNFDADSKKKITDFKKSVSDALKAANYPSKADPAQMNTVMVLVILVILVIYV
TMVYGPIAAMLVELFPTRIRYTSMSLPYHIGNGWFGGLLPTISFALVAQHGNIYYGLWYPIWIAAITFVIGALFVRETKD
VDIYRHD

Sequences:

>Translated_567_residues
MATVQSAPNAPVGRMQPSPMTREEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFSGLDPTAAFIFALLAFAAGFLVR
PFGALVFGRLGDMIGRKYTFLITILIMGTSTFIVGLLPSYTTIGAAAPVILIMLRLLQGLALGGEYGGAATYVAEHAPHG
RRGNYTSWIQTTATLGLFLSLIVILVVRELTGASFEDWGWRIPFLVSIVLLATSVYIRLSMSESPAFQKMKAEGKTSKAP
LTESFGQWRNLKIVILALVGLTAGQAVVWYTGQFYALFFLTQVLKVDAFTANVLIAVALAIGTPFFVFFGSLSDRIGRKG
IIMAGCLLAVLTYFPLFKALTHYANPALERAQQTAQITIKADPKECSFQGSPIAREVDFRSSCDIAKRTLAQSSASYESE
DAPAGTIASVTIAGREIASLNASRTADGQNFDADSKKKITDFKKSVSDALKAANYPSKADPAQMNTVMVLVILVILVIYV
TMVYGPIAAMLVELFPTRIRYTSMSLPYHIGNGWFGGLLPTISFALVAQHGNIYYGLWYPIWIAAITFVIGALFVRETKD
VDIYRHD
>Mature_566_residues
ATVQSAPNAPVGRMQPSPMTREEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFSGLDPTAAFIFALLAFAAGFLVRP
FGALVFGRLGDMIGRKYTFLITILIMGTSTFIVGLLPSYTTIGAAAPVILIMLRLLQGLALGGEYGGAATYVAEHAPHGR
RGNYTSWIQTTATLGLFLSLIVILVVRELTGASFEDWGWRIPFLVSIVLLATSVYIRLSMSESPAFQKMKAEGKTSKAPL
TESFGQWRNLKIVILALVGLTAGQAVVWYTGQFYALFFLTQVLKVDAFTANVLIAVALAIGTPFFVFFGSLSDRIGRKGI
IMAGCLLAVLTYFPLFKALTHYANPALERAQQTAQITIKADPKECSFQGSPIAREVDFRSSCDIAKRTLAQSSASYESED
APAGTIASVTIAGREIASLNASRTADGQNFDADSKKKITDFKKSVSDALKAANYPSKADPAQMNTVMVLVILVILVIYVT
MVYGPIAAMLVELFPTRIRYTSMSLPYHIGNGWFGGLLPTISFALVAQHGNIYYGLWYPIWIAAITFVIGALFVRETKDV
DIYRHD

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=326, Percent_Identity=38.3435582822086, Blast_Score=208, Evalue=8e-55,
Organism=Escherichia coli, GI1788292, Length=325, Percent_Identity=36, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1788942, Length=330, Percent_Identity=34.2424242424242, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1789941, Length=222, Percent_Identity=44.5945945945946, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI87082231, Length=222, Percent_Identity=25.2252252252252, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI87082404, Length=214, Percent_Identity=25.7009345794392, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 61787; Mature: 61656

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATVQSAPNAPVGRMQPSPMTREEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFSGL
CCCCCCCCCCCCCCCCCCCCCCHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DPTAAFIFALLAFAAGFLVRPFGALVFGRLGDMIGRKYTFLITILIMGTSTFIVGLLPSY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCH
TTIGAAAPVILIMLRLLQGLALGGEYGGAATYVAEHAPHGRRGNYTSWIQTTATLGLFLS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
LIVILVVRELTGASFEDWGWRIPFLVSIVLLATSVYIRLSMSESPAFQKMKAEGKTSKAP
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHCCCCCCCC
LTESFGQWRNLKIVILALVGLTAGQAVVWYTGQFYALFFLTQVLKVDAFTANVLIAVALA
CHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGTPFFVFFGSLSDRIGRKGIIMAGCLLAVLTYFPLFKALTHYANPALERAQQTAQITIK
HCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCEEEEEE
ADPKECSFQGSPIAREVDFRSSCDIAKRTLAQSSASYESEDAPAGTIASVTIAGREIASL
ECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHC
NASRTADGQNFDADSKKKITDFKKSVSDALKAANYPSKADPAQMNTVMVLVILVILVIYV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TMVYGPIAAMLVELFPTRIRYTSMSLPYHIGNGWFGGLLPTISFALVAQHGNIYYGLWYP
HHHHHHHHHHHHHHHHHHHEEEEECCCEECCCCCHHHHHHHHHHHHHEECCCEEEEHHHH
IWIAAITFVIGALFVRETKDVDIYRHD
HHHHHHHHHHHHHHHHCCCCCCCEECC
>Mature Secondary Structure 
ATVQSAPNAPVGRMQPSPMTREEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFSGL
CCCCCCCCCCCCCCCCCCCCCHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DPTAAFIFALLAFAAGFLVRPFGALVFGRLGDMIGRKYTFLITILIMGTSTFIVGLLPSY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCH
TTIGAAAPVILIMLRLLQGLALGGEYGGAATYVAEHAPHGRRGNYTSWIQTTATLGLFLS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
LIVILVVRELTGASFEDWGWRIPFLVSIVLLATSVYIRLSMSESPAFQKMKAEGKTSKAP
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHCCCCCCCC
LTESFGQWRNLKIVILALVGLTAGQAVVWYTGQFYALFFLTQVLKVDAFTANVLIAVALA
CHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGTPFFVFFGSLSDRIGRKGIIMAGCLLAVLTYFPLFKALTHYANPALERAQQTAQITIK
HCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCEEEEEE
ADPKECSFQGSPIAREVDFRSSCDIAKRTLAQSSASYESEDAPAGTIASVTIAGREIASL
ECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHC
NASRTADGQNFDADSKKKITDFKKSVSDALKAANYPSKADPAQMNTVMVLVILVILVIYV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TMVYGPIAAMLVELFPTRIRYTSMSLPYHIGNGWFGGLLPTISFALVAQHGNIYYGLWYP
HHHHHHHHHHHHHHHHHHHEEEEECCCEECCCCCHHHHHHHHHHHHHEECCCEEEEHHHH
IWIAAITFVIGALFVRETKDVDIYRHD
HHHHHHHHHHHHHHHHCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]