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The map label for this gene is yafJ [C]

Identifier: 17545204

GI number: 17545204

Start: 520660

End: 521490

Strand: Reverse

Name: yafJ [C]

Synonym: RSc0485

Alternate gene names: 17545204

Gene position: 521490-520660 (Counterclockwise)

Preceding gene: 17545208

Following gene: 17545196

Centisome position: 14.03

GC content: 65.34

Gene sequence:

>831_bases
ATGTGCCAGCTGCTTGGCATGAACTGCGCAGAACCAACCGACGTGACGTTCTCATTCACGGGGTTCGCCGCGCGCGGCGG
GGTCACCGACCACCACGCCGACGGCTTCGGCGTAGCGTTTTTCGAGGACAAGGCCTGCCGGCTGTTCATCGACAACCAGT
CAGCCGGCACCTCGCCGGTGGCGGAGCTGGTCAAGCGCTACCCGATCAAGTCCAAGAACGTCATCTCGCACATCCGCAAG
GCGACCCAGGGCAGCGTCCGGCTGGAGAACTGCCACCCGTTCATGCGCGAGCTGTGGGGCCGGCACTGGATCTTTGCCCA
CAACGGCGACCTCAAGCACTTCTCGCCCTTCCTCTCCGGCGTCTACCAGCCTGTGGGCGACACCGACAGCGAGCTGGCCT
TCTGCTTCATCATGCAGGCGCTGCGCAAGCGCTTCCCAGGCTCGCAGCCGCCGCTCAACGAGCTGTTCTACGCGCTGTCG
GATGTCACCCGGGAGGTGAACCGCCACGGCGTGTTCAACTACCTGCTGTGCAACGGACAGGCGCTGTTCGCACATTGCTC
GACGCGGCTGTACTACATCGTGCGGCAGTGGCCGTTCTCGACGGCGCACCTGATCGACGCCGACATGACGATCGATTTCG
CCAAGTACACCACGCCCGAGGACCGCGTCGCCGTGATCGCCACCGCGCCGCTGACCGACAACGAGATCTGGACCCCCTTC
GTGCCCGGCGAGCTGCTGATGTTCGAATCGGGCCAGGCGACCATGGCGACCAAGGTGCCGATCCCCGAAGCCGTGCAGGC
GGCCAACGCCGCCAACACGGCCTGCACCTGA

Upstream 100 bases:

>100_bases
GCCGGGCATCGTGGATGGGGTTGCGTGCACCGGGCGCTTGGCACCCCCGGCGACCCGCAGGATAATGGTTATTTTATTGC
ATCCTGTCTTTCGCCCCATC

Downstream 100 bases:

>100_bases
GATCCGCATCGCAAACGAAAAAGCCGCTCCCGAAAGAGCGGCTTTTCTTTTGCGCGGAACCCGGCGATCAGTGCGACAGG
ATCTTCGACAGGAACTGCTT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MCQLLGMNCAEPTDVTFSFTGFAARGGVTDHHADGFGVAFFEDKACRLFIDNQSAGTSPVAELVKRYPIKSKNVISHIRK
ATQGSVRLENCHPFMRELWGRHWIFAHNGDLKHFSPFLSGVYQPVGDTDSELAFCFIMQALRKRFPGSQPPLNELFYALS
DVTREVNRHGVFNYLLCNGQALFAHCSTRLYYIVRQWPFSTAHLIDADMTIDFAKYTTPEDRVAVIATAPLTDNEIWTPF
VPGELLMFESGQATMATKVPIPEAVQAANAANTACT

Sequences:

>Translated_276_residues
MCQLLGMNCAEPTDVTFSFTGFAARGGVTDHHADGFGVAFFEDKACRLFIDNQSAGTSPVAELVKRYPIKSKNVISHIRK
ATQGSVRLENCHPFMRELWGRHWIFAHNGDLKHFSPFLSGVYQPVGDTDSELAFCFIMQALRKRFPGSQPPLNELFYALS
DVTREVNRHGVFNYLLCNGQALFAHCSTRLYYIVRQWPFSTAHLIDADMTIDFAKYTTPEDRVAVIATAPLTDNEIWTPF
VPGELLMFESGQATMATKVPIPEAVQAANAANTACT
>Mature_276_residues
MCQLLGMNCAEPTDVTFSFTGFAARGGVTDHHADGFGVAFFEDKACRLFIDNQSAGTSPVAELVKRYPIKSKNVISHIRK
ATQGSVRLENCHPFMRELWGRHWIFAHNGDLKHFSPFLSGVYQPVGDTDSELAFCFIMQALRKRFPGSQPPLNELFYALS
DVTREVNRHGVFNYLLCNGQALFAHCSTRLYYIVRQWPFSTAHLIDADMTIDFAKYTTPEDRVAVIATAPLTDNEIWTPF
VPGELLMFESGQATMATKVPIPEAVQAANAANTACT

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=252, Percent_Identity=49.2063492063492, Blast_Score=271, Evalue=5e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 30736; Mature: 30736

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCQLLGMNCAEPTDVTFSFTGFAARGGVTDHHADGFGVAFFEDKACRLFIDNQSAGTSPV
CCEEECCCCCCCCCEEEEEECEEECCCCCCCCCCCEEEEEEECCEEEEEEECCCCCCCHH
AELVKRYPIKSKNVISHIRKATQGSVRLENCHPFMRELWGRHWIFAHNGDLKHFSPFLSG
HHHHHHCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHCCCEEEEEECCCCHHHHHHHHH
VYQPVGDTDSELAFCFIMQALRKRFPGSQPPLNELFYALSDVTREVNRHGVFNYLLCNGQ
HHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCC
ALFAHCSTRLYYIVRQWPFSTAHLIDADMTIDFAKYTTPEDRVAVIATAPLTDNEIWTPF
EEEEHHHHHEEEEEECCCCCCEEEEECCCEEEEHHCCCCCCCEEEEEECCCCCCCCCCCC
VPGELLMFESGQATMATKVPIPEAVQAANAANTACT
CCCEEEEEECCCCEEEEECCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MCQLLGMNCAEPTDVTFSFTGFAARGGVTDHHADGFGVAFFEDKACRLFIDNQSAGTSPV
CCEEECCCCCCCCCEEEEEECEEECCCCCCCCCCCEEEEEEECCEEEEEEECCCCCCCHH
AELVKRYPIKSKNVISHIRKATQGSVRLENCHPFMRELWGRHWIFAHNGDLKHFSPFLSG
HHHHHHCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHCCCEEEEEECCCCHHHHHHHHH
VYQPVGDTDSELAFCFIMQALRKRFPGSQPPLNELFYALSDVTREVNRHGVFNYLLCNGQ
HHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCC
ALFAHCSTRLYYIVRQWPFSTAHLIDADMTIDFAKYTTPEDRVAVIATAPLTDNEIWTPF
EEEEHHHHHEEEEEECCCCCCEEEEECCCEEEEHHCCCCCCCEEEEEECCCCCCCCCCCC
VPGELLMFESGQATMATKVPIPEAVQAANAANTACT
CCCEEEEEECCCCEEEEECCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]