Click here to switch to the map view.

The map label for this gene is prsA

Identifier: 17545114

GI number: 17545114

Start: 422019

End: 422969

Strand: Reverse

Name: prsA

Synonym: RSc0395

Alternate gene names: 17545114

Gene position: 422969-422019 (Counterclockwise)

Preceding gene: 17545115

Following gene: 17545113

Centisome position: 11.38

GC content: 64.04

Gene sequence:

>951_bases
ATGAGCAGCGAAGGCTTGATGGTCTTTACCGGCAATGCCAACCCGAAACTCGCAGAAGCTGTCGTCAAGCATCTCAACAT
CCCGCTAGGCAAGGCCCTTGTCGGCCGATTCTCGGATGGAGAAGTTCAGGTCGAGATCCAGGAAAACGTGCGCGGCAAGC
ACGTGTTCATCCTGCAATCCACCTGCGCGCCGACCAACGACAATCTGATGGAACTGATGGTGATGGTCGATGCGCTCAAG
CGCGCGTCCGCCCGTCGCATCACCGCCGCCATCCCCTATTTCGGCTATGCCCGCCAGGACCGCCGCCCGCGTTCGGCGCG
CGTGGCCATCTCGGCCAAGGTCGTGGCCAACATGCTGGAAGTCGCCGGCGTCGAGCGCGTGCTGACGATGGACCTCCACG
CCGATCAGATCCAGGGCTTCTTCGACATCCCGGTCGACAACATCTACGCGTCGCCGATCCTGCTCGAAGACCTGCGCAAG
AAGAACTACGACAACCTGCTGGTGGTGTCGCCCGACGTGGGCGGTGTGGTGCGCGCGCGCGCACTCGCCAAGCAGCTTGG
CGTCGACCTGGCGATCATCGACAAGCGCCGCCCCAAGGCCAACGTGGCCGAAGTGATGAACATCATCGGCGAGGTCGAGG
GCCGCAACTGCGTGATCATGGACGACATGATCGATACCGGCGGCACGCTGTGCAAGGCTGCGCAGGTGCTCAAGGAGCGC
GGCGCCAAGCAGGTGTTCTCCTACTGCACGCACCCGGTGCTGTCGGGTGGCGCGGCGGCCCGCATTGCCGACTCGGCGCT
GGACGAAGTGGTCGTGACCGACACCATTCCGCTGCGTGACGATGCCGCCCAGTGCGGCAAGATCCGCCAGCTGTCGACGG
CGCCGCTGCTGGCCGAGACCTTCACCCGCATCGTGCGCGGCGACTCGATCATGTCGCTGTTTGCCGAATAA

Upstream 100 bases:

>100_bases
GTCCTTCCTCCCCTGCCATTTTCTTCCTCATGTTCCCAGCAATTGCCCGGTTGGCACTCCAGCCAACCGGCGCGTCTGAA
GTCGAAAAACAGGTGCCCCG

Downstream 100 bases:

>100_bases
TTCAGCAGGGCTCGGGCATCCTGTTATAATTCAAAGCTTTACTGCGCAAATGCCGGCCTGATCGGCGGTTGCGCGATATC
CAACGGGGTGCATCGGCGCC

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSSEGLMVFTGNANPKLAEAVVKHLNIPLGKALVGRFSDGEVQVEIQENVRGKHVFILQSTCAPTNDNLMELMVMVDALK
RASARRITAAIPYFGYARQDRRPRSARVAISAKVVANMLEVAGVERVLTMDLHADQIQGFFDIPVDNIYASPILLEDLRK
KNYDNLLVVSPDVGGVVRARALAKQLGVDLAIIDKRRPKANVAEVMNIIGEVEGRNCVIMDDMIDTGGTLCKAAQVLKER
GAKQVFSYCTHPVLSGGAAARIADSALDEVVVTDTIPLRDDAAQCGKIRQLSTAPLLAETFTRIVRGDSIMSLFAE

Sequences:

>Translated_316_residues
MSSEGLMVFTGNANPKLAEAVVKHLNIPLGKALVGRFSDGEVQVEIQENVRGKHVFILQSTCAPTNDNLMELMVMVDALK
RASARRITAAIPYFGYARQDRRPRSARVAISAKVVANMLEVAGVERVLTMDLHADQIQGFFDIPVDNIYASPILLEDLRK
KNYDNLLVVSPDVGGVVRARALAKQLGVDLAIIDKRRPKANVAEVMNIIGEVEGRNCVIMDDMIDTGGTLCKAAQVLKER
GAKQVFSYCTHPVLSGGAAARIADSALDEVVVTDTIPLRDDAAQCGKIRQLSTAPLLAETFTRIVRGDSIMSLFAE
>Mature_315_residues
SSEGLMVFTGNANPKLAEAVVKHLNIPLGKALVGRFSDGEVQVEIQENVRGKHVFILQSTCAPTNDNLMELMVMVDALKR
ASARRITAAIPYFGYARQDRRPRSARVAISAKVVANMLEVAGVERVLTMDLHADQIQGFFDIPVDNIYASPILLEDLRKK
NYDNLLVVSPDVGGVVRARALAKQLGVDLAIIDKRRPKANVAEVMNIIGEVEGRNCVIMDDMIDTGGTLCKAAQVLKERG
AKQVFSYCTHPVLSGGAAARIADSALDEVVVTDTIPLRDDAAQCGKIRQLSTAPLLAETFTRIVRGDSIMSLFAE

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family

Homologues:

Organism=Homo sapiens, GI4506129, Length=313, Percent_Identity=46.6453674121406, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI84875539, Length=315, Percent_Identity=46.031746031746, Blast_Score=294, Evalue=7e-80,
Organism=Homo sapiens, GI4506127, Length=314, Percent_Identity=46.4968152866242, Blast_Score=293, Evalue=1e-79,
Organism=Homo sapiens, GI28557709, Length=314, Percent_Identity=45.5414012738853, Blast_Score=288, Evalue=5e-78,
Organism=Homo sapiens, GI4506133, Length=346, Percent_Identity=36.7052023121387, Blast_Score=204, Evalue=9e-53,
Organism=Homo sapiens, GI194018537, Length=345, Percent_Identity=37.1014492753623, Blast_Score=201, Evalue=6e-52,
Organism=Homo sapiens, GI310128524, Length=149, Percent_Identity=36.9127516778523, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI310115209, Length=149, Percent_Identity=36.9127516778523, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI310118259, Length=149, Percent_Identity=36.9127516778523, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI310119946, Length=149, Percent_Identity=36.9127516778523, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1787458, Length=310, Percent_Identity=65.4838709677419, Blast_Score=417, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17554704, Length=311, Percent_Identity=45.9807073954984, Blast_Score=294, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI25149168, Length=314, Percent_Identity=45.5414012738853, Blast_Score=293, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI17554702, Length=314, Percent_Identity=45.5414012738853, Blast_Score=293, Evalue=6e-80,
Organism=Caenorhabditis elegans, GI71989924, Length=314, Percent_Identity=45.5414012738853, Blast_Score=292, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI17570245, Length=342, Percent_Identity=34.7953216374269, Blast_Score=211, Evalue=6e-55,
Organism=Saccharomyces cerevisiae, GI6320946, Length=319, Percent_Identity=43.2601880877743, Blast_Score=269, Evalue=5e-73,
Organism=Saccharomyces cerevisiae, GI6319403, Length=320, Percent_Identity=43.75, Blast_Score=267, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6321776, Length=316, Percent_Identity=44.3037974683544, Blast_Score=260, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6322667, Length=196, Percent_Identity=41.8367346938776, Blast_Score=159, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6324511, Length=109, Percent_Identity=40.3669724770642, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI21355239, Length=313, Percent_Identity=46.3258785942492, Blast_Score=281, Evalue=3e-76,
Organism=Drosophila melanogaster, GI45551540, Length=335, Percent_Identity=43.2835820895522, Blast_Score=272, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24651458, Length=358, Percent_Identity=33.2402234636872, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24651456, Length=358, Percent_Identity=33.2402234636872, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI281362873, Length=358, Percent_Identity=33.2402234636872, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24651454, Length=358, Percent_Identity=33.2402234636872, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24651462, Length=206, Percent_Identity=37.378640776699, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24651464, Length=206, Percent_Identity=37.378640776699, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI45552010, Length=206, Percent_Identity=37.378640776699, Blast_Score=145, Evalue=5e-35,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KPRS_RALSO (Q8Y2E1)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_518516.1
- ProteinModelPortal:   Q8Y2E1
- SMR:   Q8Y2E1
- GeneID:   1219198
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc0395
- NMPDR:   fig|267608.1.peg.395
- HOGENOM:   HBG519284
- OMA:   YKTAGAD
- ProtClustDB:   PRK01259
- BioCyc:   RSOL267608:RSC0395-MONOMER
- BRENDA:   2.7.6.1
- GO:   GO:0005737
- HAMAP:   MF_00583_B
- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836
- TIGRFAMs:   TIGR01251

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.7.6.1

Molecular weight: Translated: 34314; Mature: 34183

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS00114 PRPP_SYNTHASE; PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEGLMVFTGNANPKLAEAVVKHLNIPLGKALVGRFSDGEVQVEIQENVRGKHVFILQS
CCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHCCCCCCEEEEEECCCCCCCEEEEEEC
TCAPTNDNLMELMVMVDALKRASARRITAAIPYFGYARQDRRPRSARVAISAKVVANMLE
CCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHEEEEHHHHHHHHHH
VAGVERVLTMDLHADQIQGFFDIPVDNIYASPILLEDLRKKNYDNLLVVSPDVGGVVRAR
HHCHHHEEEECCCHHHHCCEEECCHHHHCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHH
ALAKQLGVDLAIIDKRRPKANVAEVMNIIGEVEGRNCVIMDDMIDTGGTLCKAAQVLKER
HHHHHHCCCEEEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHC
GAKQVFSYCTHPVLSGGAAARIADSALDEVVVTDTIPLRDDAAQCGKIRQLSTAPLLAET
CHHHHHHHHCCHHHCCCHHHHHHHHHHHCEEEECCCCCCCCHHHHCCHHHCCCCHHHHHH
FTRIVRGDSIMSLFAE
HHHHHCCCHHHHHHCC
>Mature Secondary Structure 
SSEGLMVFTGNANPKLAEAVVKHLNIPLGKALVGRFSDGEVQVEIQENVRGKHVFILQS
CCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHCCCCCCEEEEEECCCCCCCEEEEEEC
TCAPTNDNLMELMVMVDALKRASARRITAAIPYFGYARQDRRPRSARVAISAKVVANMLE
CCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHEEEEHHHHHHHHHH
VAGVERVLTMDLHADQIQGFFDIPVDNIYASPILLEDLRKKNYDNLLVVSPDVGGVVRAR
HHCHHHEEEECCCHHHHCCEEECCHHHHCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHH
ALAKQLGVDLAIIDKRRPKANVAEVMNIIGEVEGRNCVIMDDMIDTGGTLCKAAQVLKER
HHHHHHCCCEEEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHC
GAKQVFSYCTHPVLSGGAAARIADSALDEVVVTDTIPLRDDAAQCGKIRQLSTAPLLAET
CHHHHHHHHCCHHHCCCHHHHHHHHHHHCEEEECCCCCCCCHHHHCCHHHCCCCHHHHHH
FTRIVRGDSIMSLFAE
HHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852