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The map label for this gene is ptsI [H]

Identifier: 17545067

GI number: 17545067

Start: 379790

End: 381550

Strand: Direct

Name: ptsI [H]

Synonym: RSc0348

Alternate gene names: 17545067

Gene position: 379790-381550 (Clockwise)

Preceding gene: 17545066

Following gene: 17545068

Centisome position: 10.22

GC content: 68.65

Gene sequence:

>1761_bases
ATGCCCTTTACCCTGCACGGCATCCCGGTCTCGCGTGGTATCGCCATCGGGCGTGCCCACATGCTGGCGCGCGCGGCGCT
GGACGTTTCGCACTACCTCGTCGACGAGGACAAGCTTGATGCCGAGGTGGAGCGCCTGCGCGCCGCCCGCACCGCGGTGC
GCGCCGAACTGGTCCTGCTCAAGCGCGAACTGCCGACCGATGCGCCCGAGGAAATGGGCGCCTTCCTCGATGTGCACCGG
ATGATCCTGGACGATGCGCTGCTGGCTCAGGCGCCCGAGACGCTGATCCACCAGCGCCGGTATAACGCGGAGTGGGCGCT
CACGACACAGCTCGAAGAACTGATGCGGCAGTTCGGCGAGATCGAAGACGAGTACCTGCGCGAGCGCAAGGCCGACATCG
AGCAGGTGGTCGAGCGGATCCTGAAGGCGCTGGCCGGCACGCCGGTGCTGGCTCCCGCGCCGGTGGCCGCCGGCGGCGAT
CCGGACGCCGGCATGATCGTCGTCGCCCATGACATCGCCCCGGCCGACATGCTGCAGTTCCGCGGCCAGACCTTCGCAGG
CTTCGTGACGGACCTGGGCGGGCGCACCTCGCACACGGCCATTGTCGCGCGCAGCCTCGACATCCCGGCGGCTGTGGGCG
TGCACAACGCCAGCACGCTGATCCGCCAGGACGACCGCATCATCATCGATGGCGACAACGGCATTGTCATCGTCGATCCG
AGCCTGGCCATCCTCGAGGAATACCGGCATCGCCGCAGCGAGAGCGAACTGCAGAAGAAGCGCCTCGAACGCCTGCGTCA
TACGCCCACCGTCACGCTCGACGGCACGCAGATCGACCTGCTCGCCAACATCGAGATGCCCGAGGATGCCGCCGCCGCGC
TGGCCGCCGGGGCGGTCGGCGTGGGCCTGTTCCGTTCCGAATTCCTGTTCATGAACCGGCGCGGCGCGCTGCCCGACGAG
GAGGAGCAGTTCGCCGCCTACCGCACCGCGGTCGAGTCGATGAACGGGCTGCCGGTCACCATCCGCACCATCGACATCGG
CGCCGACAAGCCGCTCGACGTGCGCGACGGTCGCGACGACCTGTTCGAGACCGCGCCCAATCCGGCGCTGGGGCTGCGCG
CGATCCGCTGGTCCCTGTCCGAGCCGGCGATGTTCCTGACGCAGCTGCGCGCGCTGCTGCGCGCATCGGCGTTCGGGCCG
GTGAGGATGCTTGTGCCGATGCTCGCGCACGCCCGCGAGATCGACCAGACGCTGGAGCTGATCGAGCGCGCCAAGGCCTC
GCTCGACGCCGACGGCATGGCCTACGATCCGACCATCAAGGTCGGCGCCATGATCGAGATTCCGGCGGCGGTGCTGATCC
TGCCGGTGTTTCTGCGCCGCATGGATTTTCTCTCCATCGGCACCAATGACCTGATCCAGTACACGCTTGCCATCGACCGC
GCCGACAATGCGGTCGCGCATCTGTACGACCCGCTGCATCCGGCGGTGCTGCAACTGATTGCGCGCACCATCCAGGAAGG
CCAGCGCGCCGGCCGGCCGGTATCGGTGTGCGGCGAGATGGCGGGGGACGCCACCATGACGCGGCTGTTGCTCGGCATGG
GGCTGACCGAGTTCTCGATGCACCCGTCGCAGCTGCTGAGCGTCAAGCAGCAGATCCTGCGCGCAGACCGTCCGCGCCTG
ATGCGCGAGGTCGACCGGCTGTTGCACGCCTGCGAGCCCACCGAGGTCCAGGACGTCCTGGGCGCGTTGGCGCGCGCCTG
A

Upstream 100 bases:

>100_bases
GCCATGGATGGCCTGGTCGCCCTGATCGGCGACCGCTTCGGCGAAGGCGAGTAACGCGCGCCTCGACGCTCCTCTTTTCA
ACGTCCCTCACGCCGCTCCC

Downstream 100 bases:

>100_bases
GCACCCGCCCGGTCTGTTGCGCCGGCACATCGACCGTGCCGGCGTTTTCCCTTTCTAAGTGATTGAAATAGCACGTCTTT
TTCCGAAAAGAGGGGCCACA

Product: phosphoenolpyruvate-protein phosphotransferase transmembrane

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I; Protein I [H]

Number of amino acids: Translated: 586; Mature: 585

Protein sequence:

>586_residues
MPFTLHGIPVSRGIAIGRAHMLARAALDVSHYLVDEDKLDAEVERLRAARTAVRAELVLLKRELPTDAPEEMGAFLDVHR
MILDDALLAQAPETLIHQRRYNAEWALTTQLEELMRQFGEIEDEYLRERKADIEQVVERILKALAGTPVLAPAPVAAGGD
PDAGMIVVAHDIAPADMLQFRGQTFAGFVTDLGGRTSHTAIVARSLDIPAAVGVHNASTLIRQDDRIIIDGDNGIVIVDP
SLAILEEYRHRRSESELQKKRLERLRHTPTVTLDGTQIDLLANIEMPEDAAAALAAGAVGVGLFRSEFLFMNRRGALPDE
EEQFAAYRTAVESMNGLPVTIRTIDIGADKPLDVRDGRDDLFETAPNPALGLRAIRWSLSEPAMFLTQLRALLRASAFGP
VRMLVPMLAHAREIDQTLELIERAKASLDADGMAYDPTIKVGAMIEIPAAVLILPVFLRRMDFLSIGTNDLIQYTLAIDR
ADNAVAHLYDPLHPAVLQLIARTIQEGQRAGRPVSVCGEMAGDATMTRLLLGMGLTEFSMHPSQLLSVKQQILRADRPRL
MREVDRLLHACEPTEVQDVLGALARA

Sequences:

>Translated_586_residues
MPFTLHGIPVSRGIAIGRAHMLARAALDVSHYLVDEDKLDAEVERLRAARTAVRAELVLLKRELPTDAPEEMGAFLDVHR
MILDDALLAQAPETLIHQRRYNAEWALTTQLEELMRQFGEIEDEYLRERKADIEQVVERILKALAGTPVLAPAPVAAGGD
PDAGMIVVAHDIAPADMLQFRGQTFAGFVTDLGGRTSHTAIVARSLDIPAAVGVHNASTLIRQDDRIIIDGDNGIVIVDP
SLAILEEYRHRRSESELQKKRLERLRHTPTVTLDGTQIDLLANIEMPEDAAAALAAGAVGVGLFRSEFLFMNRRGALPDE
EEQFAAYRTAVESMNGLPVTIRTIDIGADKPLDVRDGRDDLFETAPNPALGLRAIRWSLSEPAMFLTQLRALLRASAFGP
VRMLVPMLAHAREIDQTLELIERAKASLDADGMAYDPTIKVGAMIEIPAAVLILPVFLRRMDFLSIGTNDLIQYTLAIDR
ADNAVAHLYDPLHPAVLQLIARTIQEGQRAGRPVSVCGEMAGDATMTRLLLGMGLTEFSMHPSQLLSVKQQILRADRPRL
MREVDRLLHACEPTEVQDVLGALARA
>Mature_585_residues
PFTLHGIPVSRGIAIGRAHMLARAALDVSHYLVDEDKLDAEVERLRAARTAVRAELVLLKRELPTDAPEEMGAFLDVHRM
ILDDALLAQAPETLIHQRRYNAEWALTTQLEELMRQFGEIEDEYLRERKADIEQVVERILKALAGTPVLAPAPVAAGGDP
DAGMIVVAHDIAPADMLQFRGQTFAGFVTDLGGRTSHTAIVARSLDIPAAVGVHNASTLIRQDDRIIIDGDNGIVIVDPS
LAILEEYRHRRSESELQKKRLERLRHTPTVTLDGTQIDLLANIEMPEDAAAALAAGAVGVGLFRSEFLFMNRRGALPDEE
EQFAAYRTAVESMNGLPVTIRTIDIGADKPLDVRDGRDDLFETAPNPALGLRAIRWSLSEPAMFLTQLRALLRASAFGPV
RMLVPMLAHAREIDQTLELIERAKASLDADGMAYDPTIKVGAMIEIPAAVLILPVFLRRMDFLSIGTNDLIQYTLAIDRA
DNAVAHLYDPLHPAVLQLIARTIQEGQRAGRPVSVCGEMAGDATMTRLLLGMGLTEFSMHPSQLLSVKQQILRADRPRLM
REVDRLLHACEPTEVQDVLGALARA

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=548, Percent_Identity=39.051094890511, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1789193, Length=587, Percent_Identity=32.7086882453152, Blast_Score=271, Evalue=6e-74,
Organism=Escherichia coli, GI48994992, Length=521, Percent_Identity=33.7811900191939, Blast_Score=261, Evalue=9e-71,
Organism=Escherichia coli, GI1788726, Length=584, Percent_Identity=30.8219178082192, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI1787994, Length=404, Percent_Identity=25.2475247524752, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI226510935, Length=170, Percent_Identity=28.2352941176471, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 64442; Mature: 64311

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFTLHGIPVSRGIAIGRAHMLARAALDVSHYLVDEDKLDAEVERLRAARTAVRAELVLL
CCEEEECCCHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
KRELPTDAPEEMGAFLDVHRMILDDALLAQAPETLIHQRRYNAEWALTTQLEELMRQFGE
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEHHHHHHHHHHHHCC
IEDEYLRERKADIEQVVERILKALAGTPVLAPAPVAAGGDPDAGMIVVAHDIAPADMLQF
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHH
RGQTFAGFVTDLGGRTSHTAIVARSLDIPAAVGVHNASTLIRQDDRIIIDGDNGIVIVDP
CCCHHHHHHHHCCCCCCCCEEEEECCCCCHHHCCCCHHHHHCCCCEEEEECCCCEEEECC
SLAILEEYRHRRSESELQKKRLERLRHTPTVTLDGTQIDLLANIEMPEDAAAALAAGAVG
CHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCEEEEEEECCCCCHHHHHHHHHHHH
VGLFRSEFLFMNRRGALPDEEEQFAAYRTAVESMNGLPVTIRTIDIGADKPLDVRDGRDD
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCHH
LFETAPNPALGLRAIRWSLSEPAMFLTQLRALLRASAFGPVRMLVPMLAHAREIDQTLEL
HHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
IERAKASLDADGMAYDPTIKVGAMIEIPAAVLILPVFLRRMDFLSIGTNDLIQYTLAIDR
HHHHHHCCCCCCCEECCCEEECCEEHHHHHHHHHHHHHHHHHHHHCCCHHHHEEHHEEEC
ADNAVAHLYDPLHPAVLQLIARTIQEGQRAGRPVSVCGEMAGDATMTRLLLGMGLTEFSM
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHCCCHHHCC
HPSQLLSVKQQILRADRPRLMREVDRLLHACEPTEVQDVLGALARA
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
PFTLHGIPVSRGIAIGRAHMLARAALDVSHYLVDEDKLDAEVERLRAARTAVRAELVLL
CEEEECCCHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
KRELPTDAPEEMGAFLDVHRMILDDALLAQAPETLIHQRRYNAEWALTTQLEELMRQFGE
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEHHHHHHHHHHHHCC
IEDEYLRERKADIEQVVERILKALAGTPVLAPAPVAAGGDPDAGMIVVAHDIAPADMLQF
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHH
RGQTFAGFVTDLGGRTSHTAIVARSLDIPAAVGVHNASTLIRQDDRIIIDGDNGIVIVDP
CCCHHHHHHHHCCCCCCCCEEEEECCCCCHHHCCCCHHHHHCCCCEEEEECCCCEEEECC
SLAILEEYRHRRSESELQKKRLERLRHTPTVTLDGTQIDLLANIEMPEDAAAALAAGAVG
CHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCEEEEEEECCCCCHHHHHHHHHHHH
VGLFRSEFLFMNRRGALPDEEEQFAAYRTAVESMNGLPVTIRTIDIGADKPLDVRDGRDD
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCHH
LFETAPNPALGLRAIRWSLSEPAMFLTQLRALLRASAFGPVRMLVPMLAHAREIDQTLEL
HHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
IERAKASLDADGMAYDPTIKVGAMIEIPAAVLILPVFLRRMDFLSIGTNDLIQYTLAIDR
HHHHHHCCCCCCCEECCCEEECCEEHHHHHHHHHHHHHHHHHHHHCCCHHHHEEHHEEEC
ADNAVAHLYDPLHPAVLQLIARTIQEGQRAGRPVSVCGEMAGDATMTRLLLGMGLTEFSM
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHCCCHHHCC
HPSQLLSVKQQILRADRPRLMREVDRLLHACEPTEVQDVLGALARA
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1653223 [H]