Click here to switch to the map view.

The map label for this gene is echA12 [H]

Identifier: 17545023

GI number: 17545023

Start: 335935

End: 336753

Strand: Direct

Name: echA12 [H]

Synonym: RSc0304

Alternate gene names: 17545023

Gene position: 335935-336753 (Clockwise)

Preceding gene: 17545022

Following gene: 17545024

Centisome position: 9.04

GC content: 70.33

Gene sequence:

>819_bases
ATGAACTCGCTCGCCCCACGCTTTGCCCGTTACCAGGCGCTGACGCTCGTGCAGCACGGCCCCATCCTCGAGATCGTGAT
GGGGGCGGCGCAGTCGGCCAACCGCAAGCTGTCCACCGCCGACGCCGGTCTGCATCGCGAGCTGGCCGAGATCTGGCGCG
ACGTGTCGGCCGAGCCATCGGTGCGCGTGGCGCTGATCCGCGGCGAGGGCCGGGGCTTCTCGGCCGGCGGCGACCTGCAC
CTGGTCGAGCAGATGGCCGATGACTTCGAGGTGCGCACCCGCGTCTGGCACGAGGCGCGCGACTTGGTCTACAACCTCGT
CAACTGCGACAAGCCCGTTGTCAGCGCCATGCATGGCCCGGCGGTGGGCGCGGGGCTGGTGGCCGGCTTGCTGGCGGATA
TCTCGATCGCGGCCCGGGACGCGCGCATCGTCGACGGCCATACCCGGCTGGGCGTGGCGGCCGGGGACCATGCTGCGATC
GTCTGGCCGCTGCTGTGCGGCATGGCCAAGGCCAAGTATTACCTGCTGCTGTGCGAATCGGTGTCGGGCGAGGAGGCCGA
GCGGATCGGGCTGGTGTCCCTGGCGGTGCCGGAGGCGGAGCTGGTCAACCGCGCCTTCGAGATCGCCGAGCGGCTGGCGG
CGGGTTCGCAGACGGCCATCCGCTGGACCAAGTACGCGCTCAACAGCTGGTTGCGCCTGGCCGGGCCCACCTTCGATACC
TCGCTGGCGCTCGAATTCATGGGGTTTGCCGGGCCGGATGTGCGCGAGGGCATTGCCAGCCTGCGCCAGAAGCGGCCGCC
CGACTTCGATGCCGCCTGA

Upstream 100 bases:

>100_bases
CACCTTCCGGGTGGCCGGGCACCGCTTCCACATGGACGGGGCGGTGCCCAAGCCGGAAGTCTTCCCGATCCGCTATGCTT
GATGCACATTTCGCCCCGCC

Downstream 100 bases:

>100_bases
GCGCGGCGTTCAAAAACGATTTCCAGGTAGGAGACACCACACCATGTTCACGCAGATGCCCAACTTCGCCGAAGGCGGCT
TCGAATTCCTGCGCAAGCTC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MNSLAPRFARYQALTLVQHGPILEIVMGAAQSANRKLSTADAGLHRELAEIWRDVSAEPSVRVALIRGEGRGFSAGGDLH
LVEQMADDFEVRTRVWHEARDLVYNLVNCDKPVVSAMHGPAVGAGLVAGLLADISIAARDARIVDGHTRLGVAAGDHAAI
VWPLLCGMAKAKYYLLLCESVSGEEAERIGLVSLAVPEAELVNRAFEIAERLAAGSQTAIRWTKYALNSWLRLAGPTFDT
SLALEFMGFAGPDVREGIASLRQKRPPDFDAA

Sequences:

>Translated_272_residues
MNSLAPRFARYQALTLVQHGPILEIVMGAAQSANRKLSTADAGLHRELAEIWRDVSAEPSVRVALIRGEGRGFSAGGDLH
LVEQMADDFEVRTRVWHEARDLVYNLVNCDKPVVSAMHGPAVGAGLVAGLLADISIAARDARIVDGHTRLGVAAGDHAAI
VWPLLCGMAKAKYYLLLCESVSGEEAERIGLVSLAVPEAELVNRAFEIAERLAAGSQTAIRWTKYALNSWLRLAGPTFDT
SLALEFMGFAGPDVREGIASLRQKRPPDFDAA
>Mature_272_residues
MNSLAPRFARYQALTLVQHGPILEIVMGAAQSANRKLSTADAGLHRELAEIWRDVSAEPSVRVALIRGEGRGFSAGGDLH
LVEQMADDFEVRTRVWHEARDLVYNLVNCDKPVVSAMHGPAVGAGLVAGLLADISIAARDARIVDGHTRLGVAAGDHAAI
VWPLLCGMAKAKYYLLLCESVSGEEAERIGLVSLAVPEAELVNRAFEIAERLAAGSQTAIRWTKYALNSWLRLAGPTFDT
SLALEFMGFAGPDVREGIASLRQKRPPDFDAA

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=233, Percent_Identity=24.4635193133047, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI20127408, Length=213, Percent_Identity=27.2300469483568, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1787660, Length=250, Percent_Identity=32, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787659, Length=223, Percent_Identity=30.9417040358744, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI221142681, Length=211, Percent_Identity=29.8578199052133, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1788597, Length=226, Percent_Identity=25.6637168141593, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=238, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=231, Percent_Identity=26.8398268398268, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17554946, Length=241, Percent_Identity=26.5560165975104, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=224, Percent_Identity=25, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=183, Percent_Identity=26.2295081967213, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17560910, Length=203, Percent_Identity=25.615763546798, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI20129971, Length=242, Percent_Identity=23.9669421487603, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24653477, Length=242, Percent_Identity=23.9669421487603, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24654903, Length=192, Percent_Identity=26.0416666666667, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24653139, Length=236, Percent_Identity=26.271186440678, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19922422, Length=210, Percent_Identity=25.2380952380952, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 29307; Mature: 29307

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLAPRFARYQALTLVQHGPILEIVMGAAQSANRKLSTADAGLHRELAEIWRDVSAEPS
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCC
VRVALIRGEGRGFSAGGDLHLVEQMADDFEVRTRVWHEARDLVYNLVNCDKPVVSAMHGP
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC
AVGAGLVAGLLADISIAARDARIVDGHTRLGVAAGDHAAIVWPLLCGMAKAKYYLLLCES
CHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHHHEEEEEECC
VSGEEAERIGLVSLAVPEAELVNRAFEIAERLAAGSQTAIRWTKYALNSWLRLAGPTFDT
CCCCHHHHEEEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCH
SLALEFMGFAGPDVREGIASLRQKRPPDFDAA
HHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNSLAPRFARYQALTLVQHGPILEIVMGAAQSANRKLSTADAGLHRELAEIWRDVSAEPS
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCC
VRVALIRGEGRGFSAGGDLHLVEQMADDFEVRTRVWHEARDLVYNLVNCDKPVVSAMHGP
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC
AVGAGLVAGLLADISIAARDARIVDGHTRLGVAAGDHAAIVWPLLCGMAKAKYYLLLCES
CHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHHHEEEEEECC
VSGEEAERIGLVSLAVPEAELVNRAFEIAERLAAGSQTAIRWTKYALNSWLRLAGPTFDT
CCCCHHHHEEEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCH
SLALEFMGFAGPDVREGIASLRQKRPPDFDAA
HHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]