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The map label for this gene is metF [H]

Identifier: 17544810

GI number: 17544810

Start: 104782

End: 105612

Strand: Reverse

Name: metF [H]

Synonym: RSc0091

Alternate gene names: 17544810

Gene position: 105612-104782 (Counterclockwise)

Preceding gene: 17544811

Following gene: 17544809

Centisome position: 2.84

GC content: 67.27

Gene sequence:

>831_bases
ATGCAAGACCGTCAATTCAGTTTCGAATTCTTCCCGCCCAAGACGGCGGAAGGCGCCGAGAAGCTGCGCAACACGCGCGC
GCAGCTCAGCCCCCTCAAGCCGCGCTTCATTTCCGTGACCTTCGGGGCGGGCGGCACCACGCAGCAGGGCACGCTCGACG
CCGTGGTGGAGATCCAGCGCGAAGGCATCGAGGCCGCGCCGCACCTGTCGTGCGTGGGCTCGTCGCGCGAGAGCATCCGC
GCCATCCTCGACACGTATCGTGAGCACGGCATCCGCCGCATCGTCGCGCTGCGCGGCGACATGCCCTCGGGCATGGGCGA
GATCGGCGAGTTCCGCTTCGCCAACGAGCTGGTCGAGTTCATCCGCAAGGAGATGAACGACACCTTCCACATCGAGGTGG
CTGCGTATCCCGAGTACCACCCGCAGGCACGCTCGTCGCGCCAGGACCTGGAGAATTTCGCCCGCAAGGTCAAGGCCGGC
GCCAACTCCGCCATCACGCAGTACTTCTTCAACGCCGACGCCTACTTCCAGTTCGTCGACGACGCGCGCAAGCTGGGCGT
GGATGTGCCCATCGTGCCGGGCATCATGCCGATCACCAATTCGTCGCAGCTGATGCGCTTCTCGGAGATGTGCGGCGCGG
AGATCCCGCGCTGGATCGCCAAGCGGCTGGAGAGCTTCGGCGACGACCGCGAATCGATCCGCGCCTTCGGCCTGGACGTG
GTGACGGCGCTGTGCGACCGGCTGCTCGCGGGCGGCGCACCGGGCCTGCATTTCTACACGCTCAACGCGGCCGCCGCCAC
GCGCGCGATCTGGCAGCGGCTGGGGCTGTAG

Upstream 100 bases:

>100_bases
GTGGCCAGCTTCGGCGCAGCACTGCTGGGCTCGGTGCTGTACAGCGTGATCTCGTGGCTGCTCTCCAGCCTGCTGCTGCG
CGATTGAGCACATCTCCGTT

Downstream 100 bases:

>100_bases
CGCACGCCATGGCAACCGCCCCCGACCCGCTGATCGCCTGGCTGCTGGACGAACTGCAGCCGCTGGCCGCGCAGATCGGC
GAGATCCGCGCGCGCCGGAT

Product: 5,10-methylenetetrahydrofolate reductase oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MQDRQFSFEFFPPKTAEGAEKLRNTRAQLSPLKPRFISVTFGAGGTTQQGTLDAVVEIQREGIEAAPHLSCVGSSRESIR
AILDTYREHGIRRIVALRGDMPSGMGEIGEFRFANELVEFIRKEMNDTFHIEVAAYPEYHPQARSSRQDLENFARKVKAG
ANSAITQYFFNADAYFQFVDDARKLGVDVPIVPGIMPITNSSQLMRFSEMCGAEIPRWIAKRLESFGDDRESIRAFGLDV
VTALCDRLLAGGAPGLHFYTLNAAAATRAIWQRLGL

Sequences:

>Translated_276_residues
MQDRQFSFEFFPPKTAEGAEKLRNTRAQLSPLKPRFISVTFGAGGTTQQGTLDAVVEIQREGIEAAPHLSCVGSSRESIR
AILDTYREHGIRRIVALRGDMPSGMGEIGEFRFANELVEFIRKEMNDTFHIEVAAYPEYHPQARSSRQDLENFARKVKAG
ANSAITQYFFNADAYFQFVDDARKLGVDVPIVPGIMPITNSSQLMRFSEMCGAEIPRWIAKRLESFGDDRESIRAFGLDV
VTALCDRLLAGGAPGLHFYTLNAAAATRAIWQRLGL
>Mature_276_residues
MQDRQFSFEFFPPKTAEGAEKLRNTRAQLSPLKPRFISVTFGAGGTTQQGTLDAVVEIQREGIEAAPHLSCVGSSRESIR
AILDTYREHGIRRIVALRGDMPSGMGEIGEFRFANELVEFIRKEMNDTFHIEVAAYPEYHPQARSSRQDLENFARKVKAG
ANSAITQYFFNADAYFQFVDDARKLGVDVPIVPGIMPITNSSQLMRFSEMCGAEIPRWIAKRLESFGDDRESIRAFGLDV
VTALCDRLLAGGAPGLHFYTLNAAAATRAIWQRLGL

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=283, Percent_Identity=39.5759717314488, Blast_Score=195, Evalue=3e-50,
Organism=Escherichia coli, GI1790377, Length=271, Percent_Identity=43.9114391143911, Blast_Score=232, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI25147436, Length=282, Percent_Identity=34.3971631205674, Blast_Score=171, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI25147441, Length=282, Percent_Identity=34.3971631205674, Blast_Score=171, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6321313, Length=282, Percent_Identity=34.7517730496454, Blast_Score=164, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6325234, Length=283, Percent_Identity=34.6289752650177, Blast_Score=155, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24662658, Length=265, Percent_Identity=26.4150943396226, Blast_Score=81, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 30770; Mature: 30770

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDRQFSFEFFPPKTAEGAEKLRNTRAQLSPLKPRFISVTFGAGGTTQQGTLDAVVEIQR
CCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHH
EGIEAAPHLSCVGSSRESIRAILDTYREHGIRRIVALRGDMPSGMGEIGEFRFANELVEF
HCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
IRKEMNDTFHIEVAAYPEYHPQARSSRQDLENFARKVKAGANSAITQYFFNADAYFQFVD
HHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHH
DARKLGVDVPIVPGIMPITNSSQLMRFSEMCGAEIPRWIAKRLESFGDDRESIRAFGLDV
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
VTALCDRLLAGGAPGLHFYTLNAAAATRAIWQRLGL
HHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCC
>Mature Secondary Structure
MQDRQFSFEFFPPKTAEGAEKLRNTRAQLSPLKPRFISVTFGAGGTTQQGTLDAVVEIQR
CCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHH
EGIEAAPHLSCVGSSRESIRAILDTYREHGIRRIVALRGDMPSGMGEIGEFRFANELVEF
HCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
IRKEMNDTFHIEVAAYPEYHPQARSSRQDLENFARKVKAGANSAITQYFFNADAYFQFVD
HHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHH
DARKLGVDVPIVPGIMPITNSSQLMRFSEMCGAEIPRWIAKRLESFGDDRESIRAFGLDV
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
VTALCDRLLAGGAPGLHFYTLNAAAATRAIWQRLGL
HHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]