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The map label for this gene is topB [H]

Identifier: 17544785

GI number: 17544785

Start: 74076

End: 76709

Strand: Reverse

Name: topB [H]

Synonym: RSc0066

Alternate gene names: 17544785

Gene position: 76709-74076 (Counterclockwise)

Preceding gene: 17544786

Following gene: 17544783

Centisome position: 2.06

GC content: 65.22

Gene sequence:

>2634_bases
ATGTCCAAGGCTCTGATCATTGCCGAAAAACCGTCGGTCGCCGCTGATATTGCGCGCGCGCTGGGTGGCTTCACCAAGCA
CGACGAGTACTACGAAAGCGACGACCACGTCCTTTCGTCGGCGGTCGGCCACCTGGTCGAAATCGCGGCCCCCGATGAAT
ACGAAGTCAAGCGTGGCAAATGGAGCTTCGCGCACCTGCCGGTGATCCCGCCGCACTTCGACCTGCGCCCGATCGCCAAA
TCGGAATCGCGGCTGAAGGTTCTGACCCGGCTGATCAAGCGCAAGGACGTCACCAGCCTCATCAACGCCTGCGACGCGGG
GCGCGAGGGCGAACTGATCTTCCGCCTGATCGCGCAGCACGCCAACACCAAGCTGCCGATCCAGCGGCTGTGGCTGCAAT
CGATGACGCCGCAGGCCATCCGCGACGGCTTTGCCCAGTTGCGCAGCGACAACGAGATGCTGCCGCTGGCCGACGCCGCC
CGCTGCCGCTCCGAGGCCGACTGGCTGGTCGGCATCAACGGCACGCGCGCCATGACCGCCTTCAACAGCAAGGGCGGCGG
CTTCTTCCTGACCACCGTGGGCCGCGTGCAGACGCCGACGCTGTCGATCGTGGTCGAGCGCGAGGAGAAGATCAAGCGCT
TCGTCCCGCGCGACTACTGGGAAGTGCGCGCCGAGTTCATCGCCGCGGCCGGCCTGTACGAGGGCCGCTGGTTCGACCCG
AAGTTCAAGAAGAGCGAGTTCGACCCCGAGCAGCGCGAATCGCGGCTGTGGAGCGAGGCCGAAGCCCTGAGTATTGTCGC
CGCATGCCGCGACAAGCCGGGCATGGTCACCGAAGAATCGAAGCCGTCGACGCAGATGTCGCCGGCGCTCTTCGACCTGA
CCAGCCTGCAGCGCGAGGCCAACAGCCGCTTCGGCTTCTCGGCCAAGAACACGCTGGGCCTGGCACAGGCACTGTATGAA
AAGCACAAGGTCCTGACCTACCCGCGGACGGATTCGCGCGCGCTGCCCGAAGACTACCTGGGCACGGTGCGCCAGACGCT
GGACATGCTGGCCGACAGCTCGCACAACTACCTGCCGCACGCGAAGAAGATCCTCGCCAGCCAGTGGGTCAAGCCCAACA
AGCGGATCTTCGACAACAGCAAGATCAGCGATCACTTCGCCATCATCCCCACGCTGCAGGCGCCGAAGAACCTGTCGGAG
CCCGAGCAGAAGCTGTACGACCTGGTGGTGCGCCGCTTCCTCGCGGTGTTCTTCCCGGCGGCCGAGTACCTGGTCACCAC
GCGCGTGACCGAGGTGGCGGGCCATCACTTCAAGACCGAAGGCAAGGTCCTCGTGAACCCGGGCTGGCTGGTAGTCTACG
GCCGCGAATCGCAAAGCAAAGACGACAAGGAAAACGGCAACCTGGTGCCGGTGGCCAAGGCCGAGAAGGTCAAGACCGAC
AAGGTCGAGCCGGTCAGCCTGACCACCAAGCCGCCCGCGCGCTACAACGAAGCGACGCTGCTGTCGGCCATGGAAGGCGC
CGGCAAGCTGGTCGATGACGACGCGCTGCGCGAGGCCATGGCCGGCAAGGGCCTGGGCACGCCGGCCACGCGCGCCGCCA
TCATCGAAGGGCTGCTGACCGAGAAATACCTGCTGCGCGAAGGCCGCGAGCTGATCCCCACCGCCAAGGCGTTCCAGTTG
ATGACGCTGTTGCGCGGCCTGGGCGTGGAAGAACTGACGCAGGCCGAGCTGACCGGCGAGTGGGAGCACAAGCTCGCCCA
GATCGAGCGCGGCCGCCTGGCGCGCGACGAATTCATGCGCGAGATCGCGCAGATGACGCAGCAGATCGTCAAGCGCGCCA
AGGAATACGATAGCGACACCATCCCCGGCGACTACGCGACGCTGTCCACGCCATGCCCGCAGTGCGGCCACGTGGTCAAG
GAGAACTACCGCCGCTTCGCCTGCAGCCACTGCGATTTCTCGATCAGCAAGATCCCGGGCGGGCGCCAGTTCGAGCTGGA
AGAAGTCGAGGAGCTGCTGATCAACAAGACCATCGGTCCGTTGCAGGGCTTCCGCAGCAAGATGGGCCGGCCGTTCGCGG
CCATCCTCAAGCTGGCGCAGGAAGACGGCCACTGGAAGATGGAGTTCGACTTCGGTCAGAACGATGGCGACGAAGACGGC
GAGCCGGTCGATTTCAGCGGCCAGGAAAGCGTCGGACATTGCCCCAAGTGCAGCGGCAATGTCTACGAGCACGGCCTGAA
GTATGTGTGCGAGAACGCGGTCGCCCAGCCCAAGACGTGCGACTTCACCACCGGCAAGATCATCCTGCAGCAGGAGATCA
GCCGCGAGCAGTTGCACAAGCTGCTCGCCGAGGGCAAGACCGACCTGCTGACCGGCTTCAAGTCGGCGCGCACGGGCCGG
GTCTTCAAGGCGTTCCTGGTCAAGCAGCCGGACGGCAAGATCGGCTTCGAGTTCGAGCCGCGCGGCGAGGGCAAGGCCGC
CGCCAAGAAGACCACGACTGCAGCCAAGAAAACCGCGGCAGCCAAGGCACCGGCCAAGACTGCCGCCAAGAAGACCACGG
CAGCGGCCAAGAAGACCCCTGCGCGCAAGAAGGCGGCCACCGAGGCCGACGAGGAAAGCACCAGCGAAACCTGA

Upstream 100 bases:

>100_bases
CTTGAAGTCGGCGAGTGGAGCCATAAGACTCTACAAAACCTACGCGAACGGTCGCTAATAATAGAAGCGACCCCATTTTG
ACCCGATTGCTTGCGCTCAC

Downstream 100 bases:

>100_bases
CCTTGGCCGTCGGCCGGGCCGGCATCGATGCAAAAAAAACGGGAGGACAGTGTGCCTCCCGTTCTGCTTTGGGCGCCGCC
TGAATCGGCCGGCTGCGCGC

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 877; Mature: 876

Protein sequence:

>877_residues
MSKALIIAEKPSVAADIARALGGFTKHDEYYESDDHVLSSAVGHLVEIAAPDEYEVKRGKWSFAHLPVIPPHFDLRPIAK
SESRLKVLTRLIKRKDVTSLINACDAGREGELIFRLIAQHANTKLPIQRLWLQSMTPQAIRDGFAQLRSDNEMLPLADAA
RCRSEADWLVGINGTRAMTAFNSKGGGFFLTTVGRVQTPTLSIVVEREEKIKRFVPRDYWEVRAEFIAAAGLYEGRWFDP
KFKKSEFDPEQRESRLWSEAEALSIVAACRDKPGMVTEESKPSTQMSPALFDLTSLQREANSRFGFSAKNTLGLAQALYE
KHKVLTYPRTDSRALPEDYLGTVRQTLDMLADSSHNYLPHAKKILASQWVKPNKRIFDNSKISDHFAIIPTLQAPKNLSE
PEQKLYDLVVRRFLAVFFPAAEYLVTTRVTEVAGHHFKTEGKVLVNPGWLVVYGRESQSKDDKENGNLVPVAKAEKVKTD
KVEPVSLTTKPPARYNEATLLSAMEGAGKLVDDDALREAMAGKGLGTPATRAAIIEGLLTEKYLLREGRELIPTAKAFQL
MTLLRGLGVEELTQAELTGEWEHKLAQIERGRLARDEFMREIAQMTQQIVKRAKEYDSDTIPGDYATLSTPCPQCGHVVK
ENYRRFACSHCDFSISKIPGGRQFELEEVEELLINKTIGPLQGFRSKMGRPFAAILKLAQEDGHWKMEFDFGQNDGDEDG
EPVDFSGQESVGHCPKCSGNVYEHGLKYVCENAVAQPKTCDFTTGKIILQQEISREQLHKLLAEGKTDLLTGFKSARTGR
VFKAFLVKQPDGKIGFEFEPRGEGKAAAKKTTTAAKKTAAAKAPAKTAAKKTTAAAKKTPARKKAATEADEESTSET

Sequences:

>Translated_877_residues
MSKALIIAEKPSVAADIARALGGFTKHDEYYESDDHVLSSAVGHLVEIAAPDEYEVKRGKWSFAHLPVIPPHFDLRPIAK
SESRLKVLTRLIKRKDVTSLINACDAGREGELIFRLIAQHANTKLPIQRLWLQSMTPQAIRDGFAQLRSDNEMLPLADAA
RCRSEADWLVGINGTRAMTAFNSKGGGFFLTTVGRVQTPTLSIVVEREEKIKRFVPRDYWEVRAEFIAAAGLYEGRWFDP
KFKKSEFDPEQRESRLWSEAEALSIVAACRDKPGMVTEESKPSTQMSPALFDLTSLQREANSRFGFSAKNTLGLAQALYE
KHKVLTYPRTDSRALPEDYLGTVRQTLDMLADSSHNYLPHAKKILASQWVKPNKRIFDNSKISDHFAIIPTLQAPKNLSE
PEQKLYDLVVRRFLAVFFPAAEYLVTTRVTEVAGHHFKTEGKVLVNPGWLVVYGRESQSKDDKENGNLVPVAKAEKVKTD
KVEPVSLTTKPPARYNEATLLSAMEGAGKLVDDDALREAMAGKGLGTPATRAAIIEGLLTEKYLLREGRELIPTAKAFQL
MTLLRGLGVEELTQAELTGEWEHKLAQIERGRLARDEFMREIAQMTQQIVKRAKEYDSDTIPGDYATLSTPCPQCGHVVK
ENYRRFACSHCDFSISKIPGGRQFELEEVEELLINKTIGPLQGFRSKMGRPFAAILKLAQEDGHWKMEFDFGQNDGDEDG
EPVDFSGQESVGHCPKCSGNVYEHGLKYVCENAVAQPKTCDFTTGKIILQQEISREQLHKLLAEGKTDLLTGFKSARTGR
VFKAFLVKQPDGKIGFEFEPRGEGKAAAKKTTTAAKKTAAAKAPAKTAAKKTTAAAKKTPARKKAATEADEESTSET
>Mature_876_residues
SKALIIAEKPSVAADIARALGGFTKHDEYYESDDHVLSSAVGHLVEIAAPDEYEVKRGKWSFAHLPVIPPHFDLRPIAKS
ESRLKVLTRLIKRKDVTSLINACDAGREGELIFRLIAQHANTKLPIQRLWLQSMTPQAIRDGFAQLRSDNEMLPLADAAR
CRSEADWLVGINGTRAMTAFNSKGGGFFLTTVGRVQTPTLSIVVEREEKIKRFVPRDYWEVRAEFIAAAGLYEGRWFDPK
FKKSEFDPEQRESRLWSEAEALSIVAACRDKPGMVTEESKPSTQMSPALFDLTSLQREANSRFGFSAKNTLGLAQALYEK
HKVLTYPRTDSRALPEDYLGTVRQTLDMLADSSHNYLPHAKKILASQWVKPNKRIFDNSKISDHFAIIPTLQAPKNLSEP
EQKLYDLVVRRFLAVFFPAAEYLVTTRVTEVAGHHFKTEGKVLVNPGWLVVYGRESQSKDDKENGNLVPVAKAEKVKTDK
VEPVSLTTKPPARYNEATLLSAMEGAGKLVDDDALREAMAGKGLGTPATRAAIIEGLLTEKYLLREGRELIPTAKAFQLM
TLLRGLGVEELTQAELTGEWEHKLAQIERGRLARDEFMREIAQMTQQIVKRAKEYDSDTIPGDYATLSTPCPQCGHVVKE
NYRRFACSHCDFSISKIPGGRQFELEEVEELLINKTIGPLQGFRSKMGRPFAAILKLAQEDGHWKMEFDFGQNDGDEDGE
PVDFSGQESVGHCPKCSGNVYEHGLKYVCENAVAQPKTCDFTTGKIILQQEISREQLHKLLAEGKTDLLTGFKSARTGRV
FKAFLVKQPDGKIGFEFEPRGEGKAAAKKTTTAAKKTAAAKAPAKTAAKKTTAAAKKTPARKKAATEADEESTSET

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=664, Percent_Identity=26.0542168674699, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI4507635, Length=720, Percent_Identity=24.4444444444444, Blast_Score=114, Evalue=3e-25,
Organism=Escherichia coli, GI1788061, Length=624, Percent_Identity=34.4551282051282, Blast_Score=335, Evalue=7e-93,
Organism=Escherichia coli, GI1787529, Length=669, Percent_Identity=26.9058295964126, Blast_Score=162, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI32563869, Length=634, Percent_Identity=25.3943217665615, Blast_Score=115, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17555378, Length=635, Percent_Identity=24.7244094488189, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323263, Length=680, Percent_Identity=25.1470588235294, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24585251, Length=664, Percent_Identity=25.7530120481928, Blast_Score=102, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 97504; Mature: 97373

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKALIIAEKPSVAADIARALGGFTKHDEYYESDDHVLSSAVGHLVEIAAPDEYEVKRGK
CCCEEEEECCCCHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHEEECCCCCCHHCCCC
WSFAHLPVIPPHFDLRPIAKSESRLKVLTRLIKRKDVTSLINACDAGREGELIFRLIAQH
CCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ANTKLPIQRLWLQSMTPQAIRDGFAQLRSDNEMLPLADAARCRSEADWLVGINGTRAMTA
CCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEECCCCEEEEE
FNSKGGGFFLTTVGRVQTPTLSIVVEREEKIKRFVPRDYWEVRAEFIAAAGLYEGRWFDP
ECCCCCCEEEEECCCCCCCEEEEEEECHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCC
KFKKSEFDPEQRESRLWSEAEALSIVAACRDKPGMVTEESKPSTQMSPALFDLTSLQREA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
NSRFGFSAKNTLGLAQALYEKHKVLTYPRTDSRALPEDYLGTVRQTLDMLADSSHNYLPH
CCCCCCCCHHHHHHHHHHHHHHCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHH
AKKILASQWVKPNKRIFDNSKISDHFAIIPTLQAPKNLSEPEQKLYDLVVRRFLAVFFPA
HHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AEYLVTTRVTEVAGHHFKTEGKVLVNPGWLVVYGRESQSKDDKENGNLVPVAKAEKVKTD
HHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCCCEEEECCCCCCCCC
KVEPVSLTTKPPARYNEATLLSAMEGAGKLVDDDALREAMAGKGLGTPATRAAIIEGLLT
CCCCEEECCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHH
EKYLLREGRELIPTAKAFQLMTLLRGLGVEELTQAELTGEWEHKLAQIERGRLARDEFMR
HHHHHHCCHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHH
EIAQMTQQIVKRAKEYDSDTIPGDYATLSTPCPQCGHVVKENYRRFACSHCDFSISKIPG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHCCCC
GRQFELEEVEELLINKTIGPLQGFRSKMGRPFAAILKLAQEDGHWKMEFDFGQNDGDEDG
CCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCC
EPVDFSGQESVGHCPKCSGNVYEHGLKYVCENAVAQPKTCDFTTGKIILQQEISREQLHK
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
LLAEGKTDLLTGFKSARTGRVFKAFLVKQPDGKIGFEFEPRGEGKAAAKKTTTAAKKTAA
HHHCCCHHHHHHHHHHCCCHHHHHHHEECCCCCCCCEECCCCCCCHHHHHHHHHHHHHHH
AKAPAKTAAKKTTAAAKKTPARKKAATEADEESTSET
HCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SKALIIAEKPSVAADIARALGGFTKHDEYYESDDHVLSSAVGHLVEIAAPDEYEVKRGK
CCEEEEECCCCHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHEEECCCCCCHHCCCC
WSFAHLPVIPPHFDLRPIAKSESRLKVLTRLIKRKDVTSLINACDAGREGELIFRLIAQH
CCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ANTKLPIQRLWLQSMTPQAIRDGFAQLRSDNEMLPLADAARCRSEADWLVGINGTRAMTA
CCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEECCCCEEEEE
FNSKGGGFFLTTVGRVQTPTLSIVVEREEKIKRFVPRDYWEVRAEFIAAAGLYEGRWFDP
ECCCCCCEEEEECCCCCCCEEEEEEECHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCC
KFKKSEFDPEQRESRLWSEAEALSIVAACRDKPGMVTEESKPSTQMSPALFDLTSLQREA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
NSRFGFSAKNTLGLAQALYEKHKVLTYPRTDSRALPEDYLGTVRQTLDMLADSSHNYLPH
CCCCCCCCHHHHHHHHHHHHHHCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHH
AKKILASQWVKPNKRIFDNSKISDHFAIIPTLQAPKNLSEPEQKLYDLVVRRFLAVFFPA
HHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AEYLVTTRVTEVAGHHFKTEGKVLVNPGWLVVYGRESQSKDDKENGNLVPVAKAEKVKTD
HHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCCCEEEECCCCCCCCC
KVEPVSLTTKPPARYNEATLLSAMEGAGKLVDDDALREAMAGKGLGTPATRAAIIEGLLT
CCCCEEECCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHH
EKYLLREGRELIPTAKAFQLMTLLRGLGVEELTQAELTGEWEHKLAQIERGRLARDEFMR
HHHHHHCCHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHH
EIAQMTQQIVKRAKEYDSDTIPGDYATLSTPCPQCGHVVKENYRRFACSHCDFSISKIPG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHCCCC
GRQFELEEVEELLINKTIGPLQGFRSKMGRPFAAILKLAQEDGHWKMEFDFGQNDGDEDG
CCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCC
EPVDFSGQESVGHCPKCSGNVYEHGLKYVCENAVAQPKTCDFTTGKIILQQEISREQLHK
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
LLAEGKTDLLTGFKSARTGRVFKAFLVKQPDGKIGFEFEPRGEGKAAAKKTTTAAKKTAA
HHHCCCHHHHHHHHHHCCCHHHHHHHEECCCCCCCCEECCCCCCCHHHHHHHHHHHHHHH
AKAPAKTAAKKTTAAAKKTPARKKAATEADEESTSET
HCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA