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The map label for this gene is gatB [H]

Identifier: 17544773

GI number: 17544773

Start: 59072

End: 60538

Strand: Reverse

Name: gatB [H]

Synonym: RSc0054

Alternate gene names: 17544773

Gene position: 60538-59072 (Counterclockwise)

Preceding gene: 17544774

Following gene: 17544772

Centisome position: 1.63

GC content: 67.42

Gene sequence:

>1467_bases
ATGCAATGGGAAGTGGTGATCGGCCTCGAAACGCACGCACAGCTTTCGACCGCCTCGAAGATTTTCTCCGGCGCCTCCAC
CGCCTTCGGCGCGGCGCCCAACACGCAGGCCGCGCCGGTTGATCTGGCGCTGCCCGGCGTGCTGCCGGTGCTCAACAAGG
GCGCCGTCGAGCGCGCCATCACGTTCGGCCTGGCCATCGGGGCCAAGATCGCGTCCAAGAGCATCTTCGCGCGCAAGAAT
TACTTCTACCCCGATCTGCCCAAGGGCTACCAGATCAGCCAGTATGAGATTCCGGTGGTGCAGGGCGGCACGCTGACCAT
CCAGGTGGAAGGCAGGAACGGGCAGCCCGGCTACGAGAAGACCGTCAACCTGACGCGCGCCCACCTGGAAGAAGACGCCG
GCAAATCGCTGCACGAAGACTTCGCCGGCATGACCGGCATCGACCTGAACCGCGCCGGCACGCCGCTGCTGGAGATCGTC
ACCGAGCCCGACATGCGCAGCGCGGCCGAAGCCGTGGCGTATGCCCGCGCGCTGCACGCGCTGGTGATGTGGCTGGGCAT
CTGCGACGGCAACATGCAGGAAGGCAGCTTCCGCTGCGATGCCAACGTCTCGGTGCGCCGGGGCCCGGACGCGCCGTTCG
GCACACGCTGCGAGATCAAGAACCTGAACTCGTTCCGCTTCCTGGAAGAGGCCATCAACTACGAAGTGCGCCGCCAGATC
GAGCTGATCGAGGACGGCGGCACCGTGGTGCAGGAAACCCGCCTGTACGACCCCGACCGCAAGGAAACGCGCTCGATGCG
CAGCAAGGAAGACGCGCACGACTACCGCTACTTCCCCGACCCCGACCTGCTGCCGCTGATGATCGGCGCCGACTGGGTGG
ACGCGGTGCGCGCCACGCTGCCGGAGCTGCCCGCCGCCATGGCCGCGCGCTTCGAATCGGCCTACGGCCTGCCCCGGTAC
GACGCCAGCATCCTGACCGCCACCAAAGCCACCGCCGCCTACTTCGAGGCCGTGGTGGCCGCCGCCGGCGCGGCCAACGC
CAAGGCGGCCGCCAACTGGATCATGGGCGAGGTGGCCTCGAACCTGAACCGCGCCGGGCTGGAGATCGATGCCGCGCCGG
TGTCGCCCGTGCAACTGGCCGGGCTGCTGGCGCGCATCGCCGACGGCACCATCTCCAACAACACCGCCAAGAAGGACGTC
TTCCCCGCCATGTGGGCCGGCGAGCACGGCGGTGACGCCGACGCCATCATCGCCGAGAAGGGCCTCAAGCAGATGTCGGA
TTCCGGCGAGCTGGAGAAGATCATCGACGAGGTGCTGGCAGCCAACGCCAAGTCGGTCGAGGAATTCCGCGCCGGCAAGG
ACAAGGCTTTCAACGCGCTGGTGGGCCAGGCGATGAAGGCCACGCGCGGCAAGGCCAACCCGAGCCAGGTCAACGAACTG
CTGAAGAAGAAACTGGGCGCAGCCTGA

Upstream 100 bases:

>100_bases
AATCCGCACCCTTGAGCGCCGCCCACCTAGCAGCGCCGCACGCACGAACACGGAACACACCTAGGATTCACACCACAACA
ATCGACAGGACACACGCAAC

Downstream 100 bases:

>100_bases
TGAGCACCATGAACGCACAAGACGTCGCCAACTACCTGCAGACGCATCCGGCCTTCTTCGAAGAGCACGCCGAACTGCTG
GCCGCCATCCAACTGACCAG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MQWEVVIGLETHAQLSTASKIFSGASTAFGAAPNTQAAPVDLALPGVLPVLNKGAVERAITFGLAIGAKIASKSIFARKN
YFYPDLPKGYQISQYEIPVVQGGTLTIQVEGRNGQPGYEKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIV
TEPDMRSAAEAVAYARALHALVMWLGICDGNMQEGSFRCDANVSVRRGPDAPFGTRCEIKNLNSFRFLEEAINYEVRRQI
ELIEDGGTVVQETRLYDPDRKETRSMRSKEDAHDYRYFPDPDLLPLMIGADWVDAVRATLPELPAAMAARFESAYGLPRY
DASILTATKATAAYFEAVVAAAGAANAKAAANWIMGEVASNLNRAGLEIDAAPVSPVQLAGLLARIADGTISNNTAKKDV
FPAMWAGEHGGDADAIIAEKGLKQMSDSGELEKIIDEVLAANAKSVEEFRAGKDKAFNALVGQAMKATRGKANPSQVNEL
LKKKLGAA

Sequences:

>Translated_488_residues
MQWEVVIGLETHAQLSTASKIFSGASTAFGAAPNTQAAPVDLALPGVLPVLNKGAVERAITFGLAIGAKIASKSIFARKN
YFYPDLPKGYQISQYEIPVVQGGTLTIQVEGRNGQPGYEKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIV
TEPDMRSAAEAVAYARALHALVMWLGICDGNMQEGSFRCDANVSVRRGPDAPFGTRCEIKNLNSFRFLEEAINYEVRRQI
ELIEDGGTVVQETRLYDPDRKETRSMRSKEDAHDYRYFPDPDLLPLMIGADWVDAVRATLPELPAAMAARFESAYGLPRY
DASILTATKATAAYFEAVVAAAGAANAKAAANWIMGEVASNLNRAGLEIDAAPVSPVQLAGLLARIADGTISNNTAKKDV
FPAMWAGEHGGDADAIIAEKGLKQMSDSGELEKIIDEVLAANAKSVEEFRAGKDKAFNALVGQAMKATRGKANPSQVNEL
LKKKLGAA
>Mature_488_residues
MQWEVVIGLETHAQLSTASKIFSGASTAFGAAPNTQAAPVDLALPGVLPVLNKGAVERAITFGLAIGAKIASKSIFARKN
YFYPDLPKGYQISQYEIPVVQGGTLTIQVEGRNGQPGYEKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIV
TEPDMRSAAEAVAYARALHALVMWLGICDGNMQEGSFRCDANVSVRRGPDAPFGTRCEIKNLNSFRFLEEAINYEVRRQI
ELIEDGGTVVQETRLYDPDRKETRSMRSKEDAHDYRYFPDPDLLPLMIGADWVDAVRATLPELPAAMAARFESAYGLPRY
DASILTATKATAAYFEAVVAAAGAANAKAAANWIMGEVASNLNRAGLEIDAAPVSPVQLAGLLARIADGTISNNTAKKDV
FPAMWAGEHGGDADAIIAEKGLKQMSDSGELEKIIDEVLAANAKSVEEFRAGKDKAFNALVGQAMKATRGKANPSQVNEL
LKKKLGAA

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=498, Percent_Identity=40.5622489959839, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI115534094, Length=468, Percent_Identity=32.4786324786325, Blast_Score=229, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6319391, Length=525, Percent_Identity=30.6666666666667, Blast_Score=232, Evalue=1e-61,
Organism=Drosophila melanogaster, GI45550808, Length=494, Percent_Identity=34.6153846153846, Blast_Score=314, Evalue=8e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52343; Mature: 52343

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQWEVVIGLETHAQLSTASKIFSGASTAFGAAPNTQAAPVDLALPGVLPVLNKGAVERAI
CCEEEEEEECCCHHHHHHHHHHCCCCHHCCCCCCCCCCCCEEECCCHHHHHCCHHHHHHH
TFGLAIGAKIASKSIFARKNYFYPDLPKGYQISQYEIPVVQGGTLTIQVEGRNGQPGYEK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCEEEEEEECCCCCCCHHH
TVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTEPDMRSAAEAVAYARALHA
HHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHH
LVMWLGICDGNMQEGSFRCDANVSVRRGPDAPFGTRCEIKNLNSFRFLEEAINYEVRRQI
HHHHHHHCCCCCCCCCEEECCCCEEECCCCCCCCCEEEECCCCHHHHHHHHHCHHHHHHH
ELIEDGGTVVQETRLYDPDRKETRSMRSKEDAHDYRYFPDPDLLPLMIGADWVDAVRATL
HHEECCCCEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHH
PELPAAMAARFESAYGLPRYDASILTATKATAAYFEAVVAAAGAANAKAAANWIMGEVAS
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
NLNRAGLEIDAAPVSPVQLAGLLARIADGTISNNTAKKDVFPAMWAGEHGGDADAIIAEK
HHHHCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHCCCCCCCCHHHHHHH
GLKQMSDSGELEKIIDEVLAANAKSVEEFRAGKDKAFNALVGQAMKATRGKANPSQVNEL
HHHHHCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHH
LKKKLGAA
HHHHHCCC
>Mature Secondary Structure
MQWEVVIGLETHAQLSTASKIFSGASTAFGAAPNTQAAPVDLALPGVLPVLNKGAVERAI
CCEEEEEEECCCHHHHHHHHHHCCCCHHCCCCCCCCCCCCEEECCCHHHHHCCHHHHHHH
TFGLAIGAKIASKSIFARKNYFYPDLPKGYQISQYEIPVVQGGTLTIQVEGRNGQPGYEK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCEEEEEEECCCCCCCHHH
TVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTEPDMRSAAEAVAYARALHA
HHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHH
LVMWLGICDGNMQEGSFRCDANVSVRRGPDAPFGTRCEIKNLNSFRFLEEAINYEVRRQI
HHHHHHHCCCCCCCCCEEECCCCEEECCCCCCCCCEEEECCCCHHHHHHHHHCHHHHHHH
ELIEDGGTVVQETRLYDPDRKETRSMRSKEDAHDYRYFPDPDLLPLMIGADWVDAVRATL
HHEECCCCEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHH
PELPAAMAARFESAYGLPRYDASILTATKATAAYFEAVVAAAGAANAKAAANWIMGEVAS
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
NLNRAGLEIDAAPVSPVQLAGLLARIADGTISNNTAKKDVFPAMWAGEHGGDADAIIAEK
HHHHCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHCCCCCCCCHHHHHHH
GLKQMSDSGELEKIIDEVLAANAKSVEEFRAGKDKAFNALVGQAMKATRGKANPSQVNEL
HHHHHCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHH
LKKKLGAA
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA