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The map label for this gene is oprM [H]

Identifier: 17544728

GI number: 17544728

Start: 6899

End: 8443

Strand: Reverse

Name: oprM [H]

Synonym: RSc0009

Alternate gene names: 17544728

Gene position: 8443-6899 (Counterclockwise)

Preceding gene: 17544729

Following gene: 17544727

Centisome position: 0.23

GC content: 69.64

Gene sequence:

>1545_bases
ATGCACAAACCCATGACTTTCCGTTTGCCCGCGCTGCTGCCGGCGCTCCTATTGGCGGCCGGCGTGCTGTCCGGCTGTTC
GCTGGCGCCGCGCTATGAGCGCCTGGCCGCCCCCGTGGCCGGCGACTACCCGCAGGCGCCGGCCGGCTACGGCGTGTCCG
CGGACACCGCCAAGCCCGGCGAGACCGCGCCGCGCGCCACCGACCTCGGCTGGCGCGAGTTTTTCCCCGACCCGCGCCTG
CAGAGACTGATCGGCCTGTCGCTGGAGAACAACCGCGACCTGCGCGTCGCCATCCTCAATATCGAGGCGGCGCGCGCGCA
ATACCGCCAGCAGATCGCCGACCTGCTGCCGCCGATCAACGCGACCGCCACCTACACGCGCAGCCTGACACCCCAGTCGA
TCTCGCAGACCGGCCAGGACATCAACACCAAGCAGTATCAGTTGGGTGTCGGTATCAGCAACTACCAGGTCAACCTGTTC
AGCGTGGGCGATGTGACGTCGTCCGCGCGCGCCAGCTACCTCGCCACCGAGGAGGGCCAGCGCGCCACGCAGATCAGCCT
GATCTCGCAGGTGGCCAAGGCCTACCTGAACGAGCGCGCCTACGCCGAGCAGCTCGAGCTGGCCCAGCAGACGCTCAAGG
GCCGCGAGGACTACTACAAGCTCGCCAAGCAGCGCTTCGACGTGGGGGCCTCCTCCGCGCTGGACCTGCGCCAGAACGAG
ACGCTGGTCGAGTCGGCCCGCGTGTCGGTGGCCCAGCTGACGCGCCAGTACGCCCAGGCGACCAACGCGCTGGTGCTGCT
GGTGGGCTCGCCGCTGCCGGCCGACCTGCCGCCGCCGACCACGGTGTCGTCAGAGAAGATCGTCGCCGACATCCCGCCCG
GGCTGCCGTCCGAACTGCTGGAGCAGCGCCCCGACATCCGCCAGGCCGAGCAGAAGCTGGTCGCCGCCAACGCCAACATC
GGCGTGGCGCGCGCGGCGTTCTTCCCGAGCCTCGGGCTGACGTCGAACGTGGGGACGGCCAGCACGGCGCTGCATGACCT
GTTCAAGAGCGGGACGGGCCTGTGGTCGTTCGTGCCGAACCTGACGCTGCCGATCTTCAACTGGGGCACCAACGTCTTCA
ACCTGGACCTGACCAAGGCCAACCAGAAGATCGCGGTGGCCAACTACGAGAAGACCATCCAGACGGCCTTCCAGGAAGTG
TCCGACGCCCTCGTGGCGCGCGGCACGCTGGACGAACAGGTGGCCGCGCAGAAGCGCTTCCGCGACGCGACGGCCGAGCG
CCTCACCCTGTCGGACCAGCGCTACCGCAACGGCGTGTCGAGCTTCCTCGACGTGCTGGACGCGCAGCGCGACCTGTTCA
GCGCCGACCAGACCCTGGTGCAGACCCGCCTGGCCCGCCTGACCAACGCCGTCGATCTGTACACGGCGCTGGGCGGCGGC
CTGCAGGAGCACAGCACCACCGTGGCCGCACAGCAGGCCCGGCCGACCGCCGCACCGGGCACCACGCCCAGCGCGTCGCC
GGACATGACAACGCCGGCCAAATAA

Upstream 100 bases:

>100_bases
TGCCCGTGTTCTTCGTGGTGGTGCGCCGCATATTCAAGGGCAGTGAACGCCAGCGCCGCCTGGATGCCGCACACCTCCCG
CTGGACGAGGAAATCTGAAC

Downstream 100 bases:

>100_bases
GCCGCGCCAGCGCAGCCGAGTACCAAGCCCGCCGCCCGGCGGGCTTTTTTTCACCCTCGGCCCGGTTGCCGCTGCTGCCG
CCAGGTACTGCAGGGCCATC

Product: drug efflux lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MHKPMTFRLPALLPALLLAAGVLSGCSLAPRYERLAAPVAGDYPQAPAGYGVSADTAKPGETAPRATDLGWREFFPDPRL
QRLIGLSLENNRDLRVAILNIEAARAQYRQQIADLLPPINATATYTRSLTPQSISQTGQDINTKQYQLGVGISNYQVNLF
SVGDVTSSARASYLATEEGQRATQISLISQVAKAYLNERAYAEQLELAQQTLKGREDYYKLAKQRFDVGASSALDLRQNE
TLVESARVSVAQLTRQYAQATNALVLLVGSPLPADLPPPTTVSSEKIVADIPPGLPSELLEQRPDIRQAEQKLVAANANI
GVARAAFFPSLGLTSNVGTASTALHDLFKSGTGLWSFVPNLTLPIFNWGTNVFNLDLTKANQKIAVANYEKTIQTAFQEV
SDALVARGTLDEQVAAQKRFRDATAERLTLSDQRYRNGVSSFLDVLDAQRDLFSADQTLVQTRLARLTNAVDLYTALGGG
LQEHSTTVAAQQARPTAAPGTTPSASPDMTTPAK

Sequences:

>Translated_514_residues
MHKPMTFRLPALLPALLLAAGVLSGCSLAPRYERLAAPVAGDYPQAPAGYGVSADTAKPGETAPRATDLGWREFFPDPRL
QRLIGLSLENNRDLRVAILNIEAARAQYRQQIADLLPPINATATYTRSLTPQSISQTGQDINTKQYQLGVGISNYQVNLF
SVGDVTSSARASYLATEEGQRATQISLISQVAKAYLNERAYAEQLELAQQTLKGREDYYKLAKQRFDVGASSALDLRQNE
TLVESARVSVAQLTRQYAQATNALVLLVGSPLPADLPPPTTVSSEKIVADIPPGLPSELLEQRPDIRQAEQKLVAANANI
GVARAAFFPSLGLTSNVGTASTALHDLFKSGTGLWSFVPNLTLPIFNWGTNVFNLDLTKANQKIAVANYEKTIQTAFQEV
SDALVARGTLDEQVAAQKRFRDATAERLTLSDQRYRNGVSSFLDVLDAQRDLFSADQTLVQTRLARLTNAVDLYTALGGG
LQEHSTTVAAQQARPTAAPGTTPSASPDMTTPAK
>Mature_514_residues
MHKPMTFRLPALLPALLLAAGVLSGCSLAPRYERLAAPVAGDYPQAPAGYGVSADTAKPGETAPRATDLGWREFFPDPRL
QRLIGLSLENNRDLRVAILNIEAARAQYRQQIADLLPPINATATYTRSLTPQSISQTGQDINTKQYQLGVGISNYQVNLF
SVGDVTSSARASYLATEEGQRATQISLISQVAKAYLNERAYAEQLELAQQTLKGREDYYKLAKQRFDVGASSALDLRQNE
TLVESARVSVAQLTRQYAQATNALVLLVGSPLPADLPPPTTVSSEKIVADIPPGLPSELLEQRPDIRQAEQKLVAANANI
GVARAAFFPSLGLTSNVGTASTALHDLFKSGTGLWSFVPNLTLPIFNWGTNVFNLDLTKANQKIAVANYEKTIQTAFQEV
SDALVARGTLDEQVAAQKRFRDATAERLTLSDQRYRNGVSSFLDVLDAQRDLFSADQTLVQTRLARLTNAVDLYTALGGG
LQEHSTTVAAQQARPTAAPGTTPSASPDMTTPAK

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=471, Percent_Identity=41.4012738853503, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI2367348, Length=486, Percent_Identity=23.8683127572016, Blast_Score=102, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 55482; Mature: 55482

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKPMTFRLPALLPALLLAAGVLSGCSLAPRYERLAAPVAGDYPQAPAGYGVSADTAKPG
CCCCCEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
ETAPRATDLGWREFFPDPRLQRLIGLSLENNRDLRVAILNIEAARAQYRQQIADLLPPIN
CCCCCCCCCCHHHHCCCHHHHHHHCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCC
ATATYTRSLTPQSISQTGQDINTKQYQLGVGISNYQVNLFSVGDVTSSARASYLATEEGQ
CCHHEECCCCHHHHHHCCCCCCCCEEEEECCCCCEEEEEEEECCCCHHHHHHHHCCCCCC
RATQISLISQVAKAYLNERAYAEQLELAQQTLKGREDYYKLAKQRFDVGASSALDLRQNE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHCCH
TLVESARVSVAQLTRQYAQATNALVLLVGSPLPADLPPPTTVSSEKIVADIPPGLPSELL
HHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCHHHH
EQRPDIRQAEQKLVAANANIGVARAAFFPSLGLTSNVGTASTALHDLFKSGTGLWSFVPN
HCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCC
LTLPIFNWGTNVFNLDLTKANQKIAVANYEKTIQTAFQEVSDALVARGTLDEQVAAQKRF
CEEEEEECCCCEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RDATAERLTLSDQRYRNGVSSFLDVLDAQRDLFSADQTLVQTRLARLTNAVDLYTALGGG
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LQEHSTTVAAQQARPTAAPGTTPSASPDMTTPAK
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHKPMTFRLPALLPALLLAAGVLSGCSLAPRYERLAAPVAGDYPQAPAGYGVSADTAKPG
CCCCCEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
ETAPRATDLGWREFFPDPRLQRLIGLSLENNRDLRVAILNIEAARAQYRQQIADLLPPIN
CCCCCCCCCCHHHHCCCHHHHHHHCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCC
ATATYTRSLTPQSISQTGQDINTKQYQLGVGISNYQVNLFSVGDVTSSARASYLATEEGQ
CCHHEECCCCHHHHHHCCCCCCCCEEEEECCCCCEEEEEEEECCCCHHHHHHHHCCCCCC
RATQISLISQVAKAYLNERAYAEQLELAQQTLKGREDYYKLAKQRFDVGASSALDLRQNE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHCCH
TLVESARVSVAQLTRQYAQATNALVLLVGSPLPADLPPPTTVSSEKIVADIPPGLPSELL
HHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCHHHH
EQRPDIRQAEQKLVAANANIGVARAAFFPSLGLTSNVGTASTALHDLFKSGTGLWSFVPN
HCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCC
LTLPIFNWGTNVFNLDLTKANQKIAVANYEKTIQTAFQEVSDALVARGTLDEQVAAQKRF
CEEEEEECCCCEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RDATAERLTLSDQRYRNGVSSFLDVLDAQRDLFSADQTLVQTRLARLTNAVDLYTALGGG
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LQEHSTTVAAQQARPTAAPGTTPSASPDMTTPAK
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]