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The map label for this gene is trmE [H]

Identifier: 17544724

GI number: 17544724

Start: 3222

End: 4667

Strand: Direct

Name: trmE [H]

Synonym: RSc0005

Alternate gene names: 17544724

Gene position: 3222-4667 (Clockwise)

Preceding gene: 17544723

Following gene: 17544725

Centisome position: 0.09

GC content: 70.4

Gene sequence:

>1446_bases
ATGACCGCCTCTTCCCACGCCATGACTTCGCCGACTGTTTCCGATGCGGCCTCCGCGCCGATCCGTACCGTGCCCATCGC
GGCGATCGCCACGGCGCCCGGGCGCGGCGGCATCGGCGTCGTGCGGGTGTCCGGCCCGGATGTGCGCGCCGTGATGCAGG
CCGTGTGCGGGCGCCTCTTGCCGCCGCGCCAGGCAACCTACCTCCCCTTTCTCGACGCCGATGGCGCGGCCATTGATCGG
GGCATCGCGCTGTGGTTTCCGGCGCCGCATTCCTACACCGGCGAGGATGTGCTCGAGCTGCAGGGCCACGGCGGCCCGGT
GGTGATGCAGCTGCTGCTGTCGCGCTGCCTGCGGGCCGGCCACGGCATCGGCCTGCGCGTGGCCGAGCCCGGCGAGTTCA
CGCGCCGCGCCTTCCTCAACGACAAGCTGGACCTGGCCCAGGCCGAAGCCGTCGCCGACCTGATCGAGGCCAGCACCGAG
GCCGCCGCGCGCTCGGCCGCGCGTTCGCTCGACGGCGTGTTCTCGCAGACCGTGCACGCGCTGGTCGAGCGCGTGATCCA
CCTGCGCATGCTGGTGGAGGCGACGCTGGATTTTCCCGAGGAGGAAATCGACTTCCTCGAAGCCGCCGACGCGCGCGGCC
AGCTCGCCGACATCCGCGCACGGCTGGACGGTGTGCTGGCGCAGGCGCGCCAGGGGGCGCTGCTGCGCGAGGGCCTGCAC
GTGGTGCTGGCCGGGCAGCCCAACGTCGGCAAGTCGTCCCTGCTCAATGCGCTGGCCGGCGCCGAGCTCGCCATCGTCAC
GCCCATCGCCGGCACCACGCGCGACAAGGTGCAGCAGACCATTCAGATCGAGGGCATTCCCCTCAACATCGTCGATACCG
CCGGCCTGCGCGACACCGAAGACGAAGTCGAGCGCATCGGCATCGAACGCACCTGGGCCGCCATCGCCCGCGCAGACGTC
GTGCTGCATCTGCTGGATGCGGCCGACTACCGTGCCCACGGCCTGTCCGCCGAAGATGCCGCCATCGACGCCCGCATCGC
CGAACACGTGCCGCCGGGCGTGCCCACGCTGCGCGTCATCAACAAGATCGACCTGGCGGGCGCGGCTGTCCCGGGCCGCG
TCGATGCGCAGCCTCCCGAGGTCTGGCTGTCGGCGCGCGACGGCAGCGGCATCGAGTTGCTGCGTGCGGCGCTGCTGGAG
ATCGCCGGCTGGCAAGGCGGCGGCGAAGGCCTGTATCTGGCGCGCGAGCGGCACCTGTCGGCGCTGCGCTCGGCCAGGGA
GCACCTGACCATCGCCGCCGATCACGCGGACCAGCGGGCCCAGTCGCTCGACCTGTTTGCCGAGGAATTGCGGCTGGCGC
AGGAAGCGTTGAACAGCATCACGGGGGCGTTTTCCAGTGATGATTTGTTGGGGGTGATTTTTAGCCGGTTTTGTATTGGA
AAGTAG

Upstream 100 bases:

>100_bases
CTCGGGTGCCCTTTTTTCATGCCGGTGCGCGGCGGGCTGTCGTGCATCGTATCGAATGGACGCTGACACTTGCGCGTGCG
GCTGCCGCACAATACCGCCC

Downstream 100 bases:

>100_bases
TGAGCTGATGCAGCTCGGAGGACGACGGGCGCGGTTCGGGTGGCGTCTACCCCTCCGTTTCGACGGCTGCGTCAGCGTCT
GGAAGATCGATGCGTGCACT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MTASSHAMTSPTVSDAASAPIRTVPIAAIATAPGRGGIGVVRVSGPDVRAVMQAVCGRLLPPRQATYLPFLDADGAAIDR
GIALWFPAPHSYTGEDVLELQGHGGPVVMQLLLSRCLRAGHGIGLRVAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTE
AAARSAARSLDGVFSQTVHALVERVIHLRMLVEATLDFPEEEIDFLEAADARGQLADIRARLDGVLAQARQGALLREGLH
VVLAGQPNVGKSSLLNALAGAELAIVTPIAGTTRDKVQQTIQIEGIPLNIVDTAGLRDTEDEVERIGIERTWAAIARADV
VLHLLDAADYRAHGLSAEDAAIDARIAEHVPPGVPTLRVINKIDLAGAAVPGRVDAQPPEVWLSARDGSGIELLRAALLE
IAGWQGGGEGLYLARERHLSALRSAREHLTIAADHADQRAQSLDLFAEELRLAQEALNSITGAFSSDDLLGVIFSRFCIG
K

Sequences:

>Translated_481_residues
MTASSHAMTSPTVSDAASAPIRTVPIAAIATAPGRGGIGVVRVSGPDVRAVMQAVCGRLLPPRQATYLPFLDADGAAIDR
GIALWFPAPHSYTGEDVLELQGHGGPVVMQLLLSRCLRAGHGIGLRVAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTE
AAARSAARSLDGVFSQTVHALVERVIHLRMLVEATLDFPEEEIDFLEAADARGQLADIRARLDGVLAQARQGALLREGLH
VVLAGQPNVGKSSLLNALAGAELAIVTPIAGTTRDKVQQTIQIEGIPLNIVDTAGLRDTEDEVERIGIERTWAAIARADV
VLHLLDAADYRAHGLSAEDAAIDARIAEHVPPGVPTLRVINKIDLAGAAVPGRVDAQPPEVWLSARDGSGIELLRAALLE
IAGWQGGGEGLYLARERHLSALRSAREHLTIAADHADQRAQSLDLFAEELRLAQEALNSITGAFSSDDLLGVIFSRFCIG
K
>Mature_480_residues
TASSHAMTSPTVSDAASAPIRTVPIAAIATAPGRGGIGVVRVSGPDVRAVMQAVCGRLLPPRQATYLPFLDADGAAIDRG
IALWFPAPHSYTGEDVLELQGHGGPVVMQLLLSRCLRAGHGIGLRVAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEA
AARSAARSLDGVFSQTVHALVERVIHLRMLVEATLDFPEEEIDFLEAADARGQLADIRARLDGVLAQARQGALLREGLHV
VLAGQPNVGKSSLLNALAGAELAIVTPIAGTTRDKVQQTIQIEGIPLNIVDTAGLRDTEDEVERIGIERTWAAIARADVV
LHLLDAADYRAHGLSAEDAAIDARIAEHVPPGVPTLRVINKIDLAGAAVPGRVDAQPPEVWLSARDGSGIELLRAALLEI
AGWQGGGEGLYLARERHLSALRSAREHLTIAADHADQRAQSLDLFAEELRLAQEALNSITGAFSSDDLLGVIFSRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=481, Percent_Identity=37.8378378378378, Blast_Score=239, Evalue=4e-63,
Organism=Homo sapiens, GI193082988, Length=511, Percent_Identity=36.0078277886497, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI193082993, Length=479, Percent_Identity=36.7432150313152, Blast_Score=219, Evalue=4e-57,
Organism=Escherichia coli, GI2367268, Length=461, Percent_Identity=55.9652928416486, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI87082120, Length=125, Percent_Identity=41.6, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17507259, Length=468, Percent_Identity=29.7008547008547, Blast_Score=151, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6323665, Length=502, Percent_Identity=29.0836653386454, Blast_Score=193, Evalue=4e-50,
Organism=Drosophila melanogaster, GI45550813, Length=475, Percent_Identity=34.1052631578947, Blast_Score=212, Evalue=4e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 50910; Mature: 50778

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASSHAMTSPTVSDAASAPIRTVPIAAIATAPGRGGIGVVRVSGPDVRAVMQAVCGRLL
CCCCCCCCCCCCHHHHCCCCCEEECEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHCCC
PPRQATYLPFLDADGAAIDRGIALWFPAPHSYTGEDVLELQGHGGPVVMQLLLSRCLRAG
CCCCCCCCCEECCCCCHHHCCEEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCC
HGIGLRVAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAARSLDGVFSQTVHA
CCCEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVERVIHLRMLVEATLDFPEEEIDFLEAADARGQLADIRARLDGVLAQARQGALLREGLH
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCE
VVLAGQPNVGKSSLLNALAGAELAIVTPIAGTTRDKVQQTIQIEGIPLNIVDTAGLRDTE
EEEECCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHEEEECCCEEEEEECCCCCCCH
DEVERIGIERTWAAIARADVVLHLLDAADYRAHGLSAEDAAIDARIAEHVPPGVPTLRVI
HHHHHHCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHH
NKIDLAGAAVPGRVDAQPPEVWLSARDGSGIELLRAALLEIAGWQGGGEGLYLARERHLS
HHHHHCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEHHHHHHH
ALRSAREHLTIAADHADQRAQSLDLFAEELRLAQEALNSITGAFSSDDLLGVIFSRFCIG
HHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
K
C
>Mature Secondary Structure 
TASSHAMTSPTVSDAASAPIRTVPIAAIATAPGRGGIGVVRVSGPDVRAVMQAVCGRLL
CCCCCCCCCCCHHHHCCCCCEEECEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHCCC
PPRQATYLPFLDADGAAIDRGIALWFPAPHSYTGEDVLELQGHGGPVVMQLLLSRCLRAG
CCCCCCCCCEECCCCCHHHCCEEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCC
HGIGLRVAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAARSLDGVFSQTVHA
CCCEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVERVIHLRMLVEATLDFPEEEIDFLEAADARGQLADIRARLDGVLAQARQGALLREGLH
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCE
VVLAGQPNVGKSSLLNALAGAELAIVTPIAGTTRDKVQQTIQIEGIPLNIVDTAGLRDTE
EEEECCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHEEEECCCEEEEEECCCCCCCH
DEVERIGIERTWAAIARADVVLHLLDAADYRAHGLSAEDAAIDARIAEHVPPGVPTLRVI
HHHHHHCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHH
NKIDLAGAAVPGRVDAQPPEVWLSARDGSGIELLRAALLEIAGWQGGGEGLYLARERHLS
HHHHHCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEHHHHHHH
ALRSAREHLTIAADHADQRAQSLDLFAEELRLAQEALNSITGAFSSDDLLGVIFSRFCIG
HHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]