Click here to switch to the map view.

The map label for this gene is trmE [H]

Identifier: 17232169

GI number: 17232169

Start: 5578059

End: 5579438

Strand: Reverse

Name: trmE [H]

Synonym: all4677

Alternate gene names: 17232169

Gene position: 5579438-5578059 (Counterclockwise)

Preceding gene: 17232171

Following gene: 17232167

Centisome position: 86.99

GC content: 46.74

Gene sequence:

>1380_bases
TTGGCTATTACTGGAACTATTGCGGCGATCGCTACAGCTATTGTCCCTCAACAAGGTAGCGTCGGTATCGTGCGGGTGTC
TGGTTCCCAGGCAATAGCGATCGCCCAAACTCTGTTTGATGCGCCTGGTAAGCAAGTTTGGGAAAGTCACCGGATTCTTT
ACGGTTACATCCGCCATCCCCAAACGCGACAAATCGTAGATGAAGCCCTGTTACTGCTCATGAAAGCGCCTCGTTCTTAC
ACCCGCGAAGATGTGGTAGAGTTCCATTGCCACGGGGGAATTATTGCAGTGCAGCAGGTGTTACAATTATGTCTGGAAAG
CGGCGCTAGACTTGCTCAACCGGGGGAATTTACCCTCAGAGCCTTTTTGAATGGCAGACTAGATTTAACCCAAGCCGAGA
GCATTGCTGATTTAGTCGGTGCGCGATCGCCCCAAGCGGCTCAAACGGCTTTAGCTGGTTTACAAGGCAAATTAGCTCAT
CCCATCCGCCAGTTACGCGCTAACTGTTTGGATATTTTGGCAGAAATTGAAGCCAGGATTGATTTTGAGGAAGATTTACC
CCCGTTGGATGATGAAGCCATCATATCAGATATTGAAAACATTGCCGCAGAAATATCGCAACTACTGGCAACTAAAGACA
AAGGTGAACTACTGCGGACAGGCTTAAAAGTGGCAATCGTAGGTCGTCCGAATGTAGGTAAATCCAGCTTATTGAATGCT
TGGAGCCAGAGCGATCGCGCCATTGTCACCGACTTACCCGGCACAACCCGCGACGTTGTAGAATCGCAGTTAGTCGTCGG
AGGCATTCCCGTACAAGTGCTAGATACAGCAGGCATTCGGGAGACTAGCGACCAAGTAGAAAAGATAGGCGTAGAGCGAT
CGCGTCAAGCAGCCAACACCGCCGATTTAGTCTTGTTAACCATCGATGCTGCCACCGGTTGGACGACTGGCGACCAAGAA
ATTTATGAACAAGTAAAACACCGTCCGTTAATTCTCGTGATGAATAAAATTGATTTGGTAGAAAAACAATTAATTACATC
TTTAGAATATCCCGAAAATATTACTCAAATTGTTCACACAGCCGCAGCCCAAAAACAAGGAATTGATAGTCTAGAAACGG
CAATATTAGAGATAGTTCAAACTGGAAAAGTCCAAGCGGCTGATATGGATTTAGCCATTAATCAAAGACAAGCCGCAGCC
CTCACCCAAGCGAAAATGTCTCTAGAACAAGTGCAAGCCACAATTACCCAGCAGCTACCCCTAGATTTTTGGACAATTGA
CTTACGTGGTGCAATCCAAGCACTGGGAGAAATCACCGGGGAAGAGGTAACAGAGTCAGTGCTTGATAGGATTTTTAGTA
GATTCTGTATAGGAAAGTAA

Upstream 100 bases:

>100_bases
ATCAAATTAGTCTAAATATCTACCATTTTAAAAAAAGTACAGACGCGATTAATCGCATCTCTGACAACTGACCAATGACA
ACTGACAAATGACTCAACTT

Downstream 100 bases:

>100_bases
ATATTGTAAATAATTATCTATCGCGATCGTCTCGCATTAATTCTGTATTATCAGTCCATGTCACGTCCTTGCAGATACTT
CTACGACGCAGATGACTTGC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MAITGTIAAIATAIVPQQGSVGIVRVSGSQAIAIAQTLFDAPGKQVWESHRILYGYIRHPQTRQIVDEALLLLMKAPRSY
TREDVVEFHCHGGIIAVQQVLQLCLESGARLAQPGEFTLRAFLNGRLDLTQAESIADLVGARSPQAAQTALAGLQGKLAH
PIRQLRANCLDILAEIEARIDFEEDLPPLDDEAIISDIENIAAEISQLLATKDKGELLRTGLKVAIVGRPNVGKSSLLNA
WSQSDRAIVTDLPGTTRDVVESQLVVGGIPVQVLDTAGIRETSDQVEKIGVERSRQAANTADLVLLTIDAATGWTTGDQE
IYEQVKHRPLILVMNKIDLVEKQLITSLEYPENITQIVHTAAAQKQGIDSLETAILEIVQTGKVQAADMDLAINQRQAAA
LTQAKMSLEQVQATITQQLPLDFWTIDLRGAIQALGEITGEEVTESVLDRIFSRFCIGK

Sequences:

>Translated_459_residues
MAITGTIAAIATAIVPQQGSVGIVRVSGSQAIAIAQTLFDAPGKQVWESHRILYGYIRHPQTRQIVDEALLLLMKAPRSY
TREDVVEFHCHGGIIAVQQVLQLCLESGARLAQPGEFTLRAFLNGRLDLTQAESIADLVGARSPQAAQTALAGLQGKLAH
PIRQLRANCLDILAEIEARIDFEEDLPPLDDEAIISDIENIAAEISQLLATKDKGELLRTGLKVAIVGRPNVGKSSLLNA
WSQSDRAIVTDLPGTTRDVVESQLVVGGIPVQVLDTAGIRETSDQVEKIGVERSRQAANTADLVLLTIDAATGWTTGDQE
IYEQVKHRPLILVMNKIDLVEKQLITSLEYPENITQIVHTAAAQKQGIDSLETAILEIVQTGKVQAADMDLAINQRQAAA
LTQAKMSLEQVQATITQQLPLDFWTIDLRGAIQALGEITGEEVTESVLDRIFSRFCIGK
>Mature_458_residues
AITGTIAAIATAIVPQQGSVGIVRVSGSQAIAIAQTLFDAPGKQVWESHRILYGYIRHPQTRQIVDEALLLLMKAPRSYT
REDVVEFHCHGGIIAVQQVLQLCLESGARLAQPGEFTLRAFLNGRLDLTQAESIADLVGARSPQAAQTALAGLQGKLAHP
IRQLRANCLDILAEIEARIDFEEDLPPLDDEAIISDIENIAAEISQLLATKDKGELLRTGLKVAIVGRPNVGKSSLLNAW
SQSDRAIVTDLPGTTRDVVESQLVVGGIPVQVLDTAGIRETSDQVEKIGVERSRQAANTADLVLLTIDAATGWTTGDQEI
YEQVKHRPLILVMNKIDLVEKQLITSLEYPENITQIVHTAAAQKQGIDSLETAILEIVQTGKVQAADMDLAINQRQAAAL
TQAKMSLEQVQATITQQLPLDFWTIDLRGAIQALGEITGEEVTESVLDRIFSRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=468, Percent_Identity=34.4017094017094, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI193082993, Length=457, Percent_Identity=34.3544857768052, Blast_Score=192, Evalue=8e-49,
Organism=Homo sapiens, GI193082988, Length=496, Percent_Identity=32.258064516129, Blast_Score=182, Evalue=4e-46,
Organism=Escherichia coli, GI2367268, Length=457, Percent_Identity=37.199124726477, Blast_Score=265, Evalue=4e-72,
Organism=Escherichia coli, GI87082120, Length=155, Percent_Identity=36.7741935483871, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17507259, Length=470, Percent_Identity=30.8510638297872, Blast_Score=194, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6323665, Length=495, Percent_Identity=29.8989898989899, Blast_Score=215, Evalue=1e-56,
Organism=Drosophila melanogaster, GI45550813, Length=485, Percent_Identity=31.340206185567, Blast_Score=198, Evalue=6e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49784; Mature: 49652

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITGTIAAIATAIVPQQGSVGIVRVSGSQAIAIAQTLFDAPGKQVWESHRILYGYIRHP
CCCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHCCCCEEHHHCCC
QTRQIVDEALLLLMKAPRSYTREDVVEFHCHGGIIAVQQVLQLCLESGARLAQPGEFTLR
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHH
AFLNGRLDLTQAESIADLVGARSPQAAQTALAGLQGKLAHPIRQLRANCLDILAEIEARI
HHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHC
DFEEDLPPLDDEAIISDIENIAAEISQLLATKDKGELLRTGLKVAIVGRPNVGKSSLLNA
CCHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEECCCCCHHHHHHH
WSQSDRAIVTDLPGTTRDVVESQLVVGGIPVQVLDTAGIRETSDQVEKIGVERSRQAANT
HCCCCCEEEECCCCCHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCC
ADLVLLTIDAATGWTTGDQEIYEQVKHRPLILVMNKIDLVEKQLITSLEYPENITQIVHT
CCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEECHHHHHHHHHHHHCCCCHHHHHHHHH
AAAQKQGIDSLETAILEIVQTGKVQAADMDLAINQRQAAALTQAKMSLEQVQATITQQLP
HHHHHCCCHHHHHHHHHHHHCCCCEEECCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHCC
LDFWTIDLRGAIQALGEITGEEVTESVLDRIFSRFCIGK
CCEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AITGTIAAIATAIVPQQGSVGIVRVSGSQAIAIAQTLFDAPGKQVWESHRILYGYIRHP
CCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHCCCCEEHHHCCC
QTRQIVDEALLLLMKAPRSYTREDVVEFHCHGGIIAVQQVLQLCLESGARLAQPGEFTLR
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHH
AFLNGRLDLTQAESIADLVGARSPQAAQTALAGLQGKLAHPIRQLRANCLDILAEIEARI
HHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHC
DFEEDLPPLDDEAIISDIENIAAEISQLLATKDKGELLRTGLKVAIVGRPNVGKSSLLNA
CCHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEECCCCCHHHHHHH
WSQSDRAIVTDLPGTTRDVVESQLVVGGIPVQVLDTAGIRETSDQVEKIGVERSRQAANT
HCCCCCEEEECCCCCHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCC
ADLVLLTIDAATGWTTGDQEIYEQVKHRPLILVMNKIDLVEKQLITSLEYPENITQIVHT
CCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEECHHHHHHHHHHHHCCCCHHHHHHHHH
AAAQKQGIDSLETAILEIVQTGKVQAADMDLAINQRQAAALTQAKMSLEQVQATITQQLP
HHHHHCCCHHHHHHHHHHHHCCCCEEECCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHCC
LDFWTIDLRGAIQALGEITGEEVTESVLDRIFSRFCIGK
CCEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA