Click here to switch to the map view.

The map label for this gene is pnp [H]

Identifier: 17231888

GI number: 17231888

Start: 5269212

End: 5271368

Strand: Reverse

Name: pnp [H]

Synonym: all4396

Alternate gene names: 17231888

Gene position: 5271368-5269212 (Counterclockwise)

Preceding gene: 17231889

Following gene: 17231887

Centisome position: 82.19

GC content: 48.4

Gene sequence:

>2157_bases
ATGGCAGAATTTGAGAAGTCAATATCCTTCGATGGAAGGGATATTCGACTGAAAGTAGGCCTACTAGCACCCCAAGCTGG
TGGGTCGGTTTTGATAGAATCAGGGGATACAGCTGTTTTAGTCACAGCTACGCGATCGCCAGGTAGAGAAGGCATTGATT
TCCTTCCCCTGACAGTAGACTATGAAGAAAGACTATATGCGGCTGGTAGAATCCCCGGTGGGATTATGCGCCGAGAAGGC
CGTCCCCCAGAAAAAACAATTCTCACCAGCCGTCTCATAGATCGTCCCCTGCGTCCTTTGTTCCCTTCATGGTTGCGGGA
TGACTTGCAAGTTGTCGCCTTAACAATGTCGATGGACGAGCAAGTACCACCCGACGTGCTAGCGGTAACAGGTGCTTCCA
TTGCTACTCTCATTGCCAAAATCCCCTTTAACGGGCCAATGGCAGCAGTGAGGGTAGGGTTAGTGGGCGATGATTTCATT
ATTAACCCCACCTATGCAGAAATCGAAGCCGGAGACTTGGATCTGGTAGTCGCAGGTTCACCTCACGGTGTGATTATGGT
GGAAGCAGGGGCTAATCAATTACCAGAGCGAGATATTATCGAGGCGATTGATTTTGGTTATGAGGCTGTGCGCGACTTAA
TTAAAGCGCAGCTAGACCTAGTAGCCGAACTCGGTCTAGAAATTGTCCAAGAAGCACCGCCAGAGGTAGACCAAACTCTG
GAAAATTATATCCGTGATCGCGCTAGCGACGAAATTAAGAAAATTCTGGCTCAATTTGAACTAACCAAACCCGAACGCGA
TGCCGCGTTGGACGTTGTTAAAGATAATATTGCCACAGCGATCGCCGAACTCCCAGAGGAAGACCCAATTCGTTTAGCTG
CAACTGCCAACAGCAAAGCCCTGGGCAACACTTTTAAAGACATCACTAAATACTTCATGCGCCGTCAAATCGTCGAAGAT
AACGTGCGTGTGGATGGTCGGAAACTTGATCAAGTGCGTCCGGTTTCTTCCCAAGTTGGTGTCTTACCAAAACGTGTCCA
CGGTAGCGGTTTATTTAACCGAGGGCTGACTCAGGTATTATCAGCTTGTACTTTGGGTACTCCTGGTGATGCCCAAAACC
TCAATGATGACCTGCAAACAGACCAATCGAAACGCTACCTCCATCATTACAACTTCCCGCCTTTCTCCGTCGGGGAAACT
AAACCCTTACGCGCCCCTGGTAGACGGGAAATTGGTCACGGTGCATTAGCAGAGAGAGCCATCTTACCTGTGCTGCCACC
AAAAGAGCAATTCCCTTACGTGATTCGCGTCGTCTCGGAAGTGCTTTCCTCCAACGGTTCCACCTCAATGGGTTCAGTTT
GTGGTTCCACCTTGGCATTAATGGATGCCGGTGTACCCATTCTCAAACCCGTGAGTGGTGCAGCAATGGGTTTGATTAAA
GAAGGTGATGAAGTCCGAGTCCTGACAGATATTCAGGGCATCGAAGACTTTTTAGGTGATATGGACTTTAAAGTCGCTGG
TACCGATGCAGGGATTACCGCCTTGCAAATGGACATGAAAATCTCTGGTTTGTCTTTGGAGGTTATTGCCCAAGCCATTC
ACCAAGCAAAAGATGCTAGGCTGCATATTTTGGACAAAATGCTCCAAACTATCGACCAACCACGCACTGAAACCTCACCT
TATGCCCCACGCCTGTTGACTATCAAGATTGATCCAGACATGATCGGTCTAGTCATTGGCCCCGGCGGTAAGACAATTAA
GGGCATCACCGAAGAAACTGGTGCAAAAATCGACATTGAAGACGATGGCACAGTGACAATTTCTGCGGTTGATGAAAACA
AGGCGAAAAGAGCGCGTAATATTGTCCAAGGCATGACTCGCAAACTCAACGAAGGCGATGTCTATGCCGGACGTGTGACT
CGGATTATTCCCATTGGTGCTTTTGTGGAATTTCTCCCTGGAAAAGAAGGGATGATTCACATTTCCCAATTAGCTGATTA
CCGTGTCGGCAAAGTTGAAGATGAAGTAGCTGTTGGCGATGAAGTAATTGTCAAAGTGCGCGAAATTGACAATAAGGGAC
GGATTAATCTCACACGCTTGGGTATACACCCAGATCAAGCAGCTGCGGCGCGGGAAGCTGCGGCGGTGAATCGTTAA

Upstream 100 bases:

>100_bases
CCGATGTTGCTCTTTGTATGTAACAGAAGCCAGCCATAGGGCATAAATGTTAGGATTGCCACAGAGAGAGAATAGCGTGC
ATAGAAGGAAAAAAAGCTGA

Downstream 100 bases:

>100_bases
ATCGATTGGGGATTTTAGATCATATCTAAAATCCGCGATGCCTCTTTACGTAGGCATCGCCGTAAAGTTTTTCATGATTG
TGAATAATCCCAAGTAGAGA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 718; Mature: 717

Protein sequence:

>718_residues
MAEFEKSISFDGRDIRLKVGLLAPQAGGSVLIESGDTAVLVTATRSPGREGIDFLPLTVDYEERLYAAGRIPGGIMRREG
RPPEKTILTSRLIDRPLRPLFPSWLRDDLQVVALTMSMDEQVPPDVLAVTGASIATLIAKIPFNGPMAAVRVGLVGDDFI
INPTYAEIEAGDLDLVVAGSPHGVIMVEAGANQLPERDIIEAIDFGYEAVRDLIKAQLDLVAELGLEIVQEAPPEVDQTL
ENYIRDRASDEIKKILAQFELTKPERDAALDVVKDNIATAIAELPEEDPIRLAATANSKALGNTFKDITKYFMRRQIVED
NVRVDGRKLDQVRPVSSQVGVLPKRVHGSGLFNRGLTQVLSACTLGTPGDAQNLNDDLQTDQSKRYLHHYNFPPFSVGET
KPLRAPGRREIGHGALAERAILPVLPPKEQFPYVIRVVSEVLSSNGSTSMGSVCGSTLALMDAGVPILKPVSGAAMGLIK
EGDEVRVLTDIQGIEDFLGDMDFKVAGTDAGITALQMDMKISGLSLEVIAQAIHQAKDARLHILDKMLQTIDQPRTETSP
YAPRLLTIKIDPDMIGLVIGPGGKTIKGITEETGAKIDIEDDGTVTISAVDENKAKRARNIVQGMTRKLNEGDVYAGRVT
RIIPIGAFVEFLPGKEGMIHISQLADYRVGKVEDEVAVGDEVIVKVREIDNKGRINLTRLGIHPDQAAAAREAAAVNR

Sequences:

>Translated_718_residues
MAEFEKSISFDGRDIRLKVGLLAPQAGGSVLIESGDTAVLVTATRSPGREGIDFLPLTVDYEERLYAAGRIPGGIMRREG
RPPEKTILTSRLIDRPLRPLFPSWLRDDLQVVALTMSMDEQVPPDVLAVTGASIATLIAKIPFNGPMAAVRVGLVGDDFI
INPTYAEIEAGDLDLVVAGSPHGVIMVEAGANQLPERDIIEAIDFGYEAVRDLIKAQLDLVAELGLEIVQEAPPEVDQTL
ENYIRDRASDEIKKILAQFELTKPERDAALDVVKDNIATAIAELPEEDPIRLAATANSKALGNTFKDITKYFMRRQIVED
NVRVDGRKLDQVRPVSSQVGVLPKRVHGSGLFNRGLTQVLSACTLGTPGDAQNLNDDLQTDQSKRYLHHYNFPPFSVGET
KPLRAPGRREIGHGALAERAILPVLPPKEQFPYVIRVVSEVLSSNGSTSMGSVCGSTLALMDAGVPILKPVSGAAMGLIK
EGDEVRVLTDIQGIEDFLGDMDFKVAGTDAGITALQMDMKISGLSLEVIAQAIHQAKDARLHILDKMLQTIDQPRTETSP
YAPRLLTIKIDPDMIGLVIGPGGKTIKGITEETGAKIDIEDDGTVTISAVDENKAKRARNIVQGMTRKLNEGDVYAGRVT
RIIPIGAFVEFLPGKEGMIHISQLADYRVGKVEDEVAVGDEVIVKVREIDNKGRINLTRLGIHPDQAAAAREAAAVNR
>Mature_717_residues
AEFEKSISFDGRDIRLKVGLLAPQAGGSVLIESGDTAVLVTATRSPGREGIDFLPLTVDYEERLYAAGRIPGGIMRREGR
PPEKTILTSRLIDRPLRPLFPSWLRDDLQVVALTMSMDEQVPPDVLAVTGASIATLIAKIPFNGPMAAVRVGLVGDDFII
NPTYAEIEAGDLDLVVAGSPHGVIMVEAGANQLPERDIIEAIDFGYEAVRDLIKAQLDLVAELGLEIVQEAPPEVDQTLE
NYIRDRASDEIKKILAQFELTKPERDAALDVVKDNIATAIAELPEEDPIRLAATANSKALGNTFKDITKYFMRRQIVEDN
VRVDGRKLDQVRPVSSQVGVLPKRVHGSGLFNRGLTQVLSACTLGTPGDAQNLNDDLQTDQSKRYLHHYNFPPFSVGETK
PLRAPGRREIGHGALAERAILPVLPPKEQFPYVIRVVSEVLSSNGSTSMGSVCGSTLALMDAGVPILKPVSGAAMGLIKE
GDEVRVLTDIQGIEDFLGDMDFKVAGTDAGITALQMDMKISGLSLEVIAQAIHQAKDARLHILDKMLQTIDQPRTETSPY
APRLLTIKIDPDMIGLVIGPGGKTIKGITEETGAKIDIEDDGTVTISAVDENKAKRARNIVQGMTRKLNEGDVYAGRVTR
IIPIGAFVEFLPGKEGMIHISQLADYRVGKVEDEVAVGDEVIVKVREIDNKGRINLTRLGIHPDQAAAAREAAAVNR

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=725, Percent_Identity=36.8275862068965, Blast_Score=437, Evalue=1e-122,
Organism=Escherichia coli, GI145693187, Length=696, Percent_Identity=48.5632183908046, Blast_Score=620, Evalue=1e-179,
Organism=Caenorhabditis elegans, GI115534063, Length=675, Percent_Identity=34.0740740740741, Blast_Score=359, Evalue=4e-99,
Organism=Drosophila melanogaster, GI281362905, Length=698, Percent_Identity=37.3925501432665, Blast_Score=435, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24651641, Length=698, Percent_Identity=37.3925501432665, Blast_Score=435, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24651643, Length=698, Percent_Identity=37.3925501432665, Blast_Score=435, Evalue=1e-122,
Organism=Drosophila melanogaster, GI161079377, Length=642, Percent_Identity=37.0716510903427, Blast_Score=400, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 77791; Mature: 77660

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEFEKSISFDGRDIRLKVGLLAPQAGGSVLIESGDTAVLVTATRSPGREGIDFLPLTVD
CCCCCCCCCCCCCEEEEEEEEECCCCCCEEEEECCCEEEEEEECCCCCCCCCEEEEEEEC
YEERLYAAGRIPGGIMRREGRPPEKTILTSRLIDRPLRPLFPSWLRDDLQVVALTMSMDE
HHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCHHCCHHHHHCCCEEEEEEECCCC
QVPPDVLAVTGASIATLIAKIPFNGPMAAVRVGLVGDDFIINPTYAEIEAGDLDLVVAGS
CCCCCCEEECCHHHHHHHHHCCCCCCHHHEEEEEECCCEEECCCCCEEECCCEEEEEECC
PHGVIMVEAGANQLPERDIIEAIDFGYEAVRDLIKAQLDLVAELGLEIVQEAPPEVDQTL
CCCEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ENYIRDRASDEIKKILAQFELTKPERDAALDVVKDNIATAIAELPEEDPIRLAATANSKA
HHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCH
LGNTFKDITKYFMRRQIVEDNVRVDGRKLDQVRPVSSQVGVLPKRVHGSGLFNRGLTQVL
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCHHHHCCCCHHHCCCCHHHHHHHHHH
SACTLGTPGDAQNLNDDLQTDQSKRYLHHYNFPPFSVGETKPLRAPGRREIGHGALAERA
HHHCCCCCCCCCCCCHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCC
ILPVLPPKEQFPYVIRVVSEVLSSNGSTSMGSVCGSTLALMDAGVPILKPVSGAAMGLIK
CCCCCCCCHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCC
EGDEVRVLTDIQGIEDFLGDMDFKVAGTDAGITALQMDMKISGLSLEVIAQAIHQAKDAR
CCCCEEEEEHHHHHHHHHCCCCEEEECCCCCCEEEEEECEECCCHHHHHHHHHHHHHHHH
LHILDKMLQTIDQPRTETSPYAPRLLTIKIDPDMIGLVIGPGGKTIKGITEETGAKIDIE
HHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCEEEEEECCCCCEECCCHHCCCCEEEEC
DDGTVTISAVDENKAKRARNIVQGMTRKLNEGDVYAGRVTRIIPIGAFVEFLPGKEGMIH
CCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEECCEEEEEEHHHHHHHCCCCCCCEE
ISQLADYRVGKVEDEVAVGDEVIVKVREIDNKGRINLTRLGIHPDQAAAAREAAAVNR
HHHHHCCCCCCCCCHHHCCHHHEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AEFEKSISFDGRDIRLKVGLLAPQAGGSVLIESGDTAVLVTATRSPGREGIDFLPLTVD
CCCCCCCCCCCCEEEEEEEEECCCCCCEEEEECCCEEEEEEECCCCCCCCCEEEEEEEC
YEERLYAAGRIPGGIMRREGRPPEKTILTSRLIDRPLRPLFPSWLRDDLQVVALTMSMDE
HHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCHHCCHHHHHCCCEEEEEEECCCC
QVPPDVLAVTGASIATLIAKIPFNGPMAAVRVGLVGDDFIINPTYAEIEAGDLDLVVAGS
CCCCCCEEECCHHHHHHHHHCCCCCCHHHEEEEEECCCEEECCCCCEEECCCEEEEEECC
PHGVIMVEAGANQLPERDIIEAIDFGYEAVRDLIKAQLDLVAELGLEIVQEAPPEVDQTL
CCCEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ENYIRDRASDEIKKILAQFELTKPERDAALDVVKDNIATAIAELPEEDPIRLAATANSKA
HHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCH
LGNTFKDITKYFMRRQIVEDNVRVDGRKLDQVRPVSSQVGVLPKRVHGSGLFNRGLTQVL
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCHHHHCCCCHHHCCCCHHHHHHHHHH
SACTLGTPGDAQNLNDDLQTDQSKRYLHHYNFPPFSVGETKPLRAPGRREIGHGALAERA
HHHCCCCCCCCCCCCHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCC
ILPVLPPKEQFPYVIRVVSEVLSSNGSTSMGSVCGSTLALMDAGVPILKPVSGAAMGLIK
CCCCCCCCHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCC
EGDEVRVLTDIQGIEDFLGDMDFKVAGTDAGITALQMDMKISGLSLEVIAQAIHQAKDAR
CCCCEEEEEHHHHHHHHHCCCCEEEECCCCCCEEEEEECEECCCHHHHHHHHHHHHHHHH
LHILDKMLQTIDQPRTETSPYAPRLLTIKIDPDMIGLVIGPGGKTIKGITEETGAKIDIE
HHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCEEEEEECCCCCEECCCHHCCCCEEEEC
DDGTVTISAVDENKAKRARNIVQGMTRKLNEGDVYAGRVTRIIPIGAFVEFLPGKEGMIH
CCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEECCEEEEEEHHHHHHHCCCCCCCEE
ISQLADYRVGKVEDEVAVGDEVIVKVREIDNKGRINLTRLGIHPDQAAAAREAAAVNR
HHHHHCCCCCCCCCHHHCCHHHEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA