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The map label for this gene is clpR [H]

Identifier: 17231850

GI number: 17231850

Start: 5220184

End: 5220846

Strand: Reverse

Name: clpR [H]

Synonym: all4358

Alternate gene names: 17231850

Gene position: 5220846-5220184 (Counterclockwise)

Preceding gene: 17231853

Following gene: 17231849

Centisome position: 81.4

GC content: 46.15

Gene sequence:

>663_bases
ATGGACATTTCCCCCATCAAGGCTGTTCAAGCCCCTTACTACGGTGACAGCTCCTACAGGACACCGCCACCAGATTTACC
CTCCCTTTTACTCAAGGAACGAATTGTCTATCTGGGGATGCCACTGGTTCCTGCTGTTACGGAATTGATAGTCGCTCAAC
TATTGTATTTACAGTCTGACGACCCCGACAAACCAATTAAAATCTATATAAACTCCACTGGCACTTCCGGTTATAGTGGC
GAACCTATCGGCTTTGAAACTGAAGCCTTCGCCATCTATGACACCATGAAATACATCAAGCCTCCAATTCACACCATTTG
CATTGGTTCGGCGATGGGTATGGCAGCAATGTTACTGAGTGCTGGGACAAAAGGTTGTCGCGCTAGTTTACCCAGCTCCT
CAATTATCCTGCACCAACCCAAGAGCTACGCCCAAGGTCAAGCAACGGATATTCAAATTCGAGCCAGGGAAGTCTTGGTA
AACAAAGGTTCAATGGTTGATATTTTCGCCCGCACCACCGGACAGGCACCAGAAAAAATCACCAAAGACATGGATCGTCT
CCTATACATGACTCCTTATCAAGCCAAGGATTACGGTTTGATTGATAGAGTTTTTGAAAAAGAAGAACTCGCAAATCCGC
CACTACCAGCAAGTGTTCTTTAA

Upstream 100 bases:

>100_bases
CTGTTACCTTTAAATTAGCGAGAAAACCGCACTTGAAGAGAGCTAGCATCGCGCTAGGATGTATTACAGTTAACGGCACA
ATATCAGGCGATCAGTTGCT

Downstream 100 bases:

>100_bases
AAAGTCAGCAGTCAATAGTTATAAAACTAATGACTAATGACTAATGACTAATGACTAAATTACAAACGGAGTCAAAAAAA
TGCCTATAGGAGTTCCTAAA

Product: ATP-dependent Clp protease-like protein

Products: NA

Alternate protein names: Endopeptidase Clp-like [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MDISPIKAVQAPYYGDSSYRTPPPDLPSLLLKERIVYLGMPLVPAVTELIVAQLLYLQSDDPDKPIKIYINSTGTSGYSG
EPIGFETEAFAIYDTMKYIKPPIHTICIGSAMGMAAMLLSAGTKGCRASLPSSSIILHQPKSYAQGQATDIQIRAREVLV
NKGSMVDIFARTTGQAPEKITKDMDRLLYMTPYQAKDYGLIDRVFEKEELANPPLPASVL

Sequences:

>Translated_220_residues
MDISPIKAVQAPYYGDSSYRTPPPDLPSLLLKERIVYLGMPLVPAVTELIVAQLLYLQSDDPDKPIKIYINSTGTSGYSG
EPIGFETEAFAIYDTMKYIKPPIHTICIGSAMGMAAMLLSAGTKGCRASLPSSSIILHQPKSYAQGQATDIQIRAREVLV
NKGSMVDIFARTTGQAPEKITKDMDRLLYMTPYQAKDYGLIDRVFEKEELANPPLPASVL
>Mature_220_residues
MDISPIKAVQAPYYGDSSYRTPPPDLPSLLLKERIVYLGMPLVPAVTELIVAQLLYLQSDDPDKPIKIYINSTGTSGYSG
EPIGFETEAFAIYDTMKYIKPPIHTICIGSAMGMAAMLLSAGTKGCRASLPSSSIILHQPKSYAQGQATDIQIRAREVLV
NKGSMVDIFARTTGQAPEKITKDMDRLLYMTPYQAKDYGLIDRVFEKEELANPPLPASVL

Specific function: Has lost the two conserved residues (Ser and His) proposed to be part of the active site. Therefore it could be inactive [H]

COG id: COG0740

COG function: function code OU; Protease subunit of ATP-dependent Clp proteases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S14 family [H]

Homologues:

Organism=Homo sapiens, GI5174419, Length=190, Percent_Identity=45.2631578947368, Blast_Score=161, Evalue=5e-40,
Organism=Escherichia coli, GI1786641, Length=181, Percent_Identity=44.7513812154696, Blast_Score=161, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17538017, Length=193, Percent_Identity=39.8963730569948, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI20129427, Length=182, Percent_Identity=45.0549450549451, Blast_Score=159, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001907 [H]

Pfam domain/function: PF00574 CLP_protease [H]

EC number: 3.4.21.92

Molecular weight: Translated: 24105; Mature: 24105

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDISPIKAVQAPYYGDSSYRTPPPDLPSLLLKERIVYLGMPLVPAVTELIVAQLLYLQSD
CCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
DPDKPIKIYINSTGTSGYSGEPIGFETEAFAIYDTMKYIKPPIHTICIGSAMGMAAMLLS
CCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AGTKGCRASLPSSSIILHQPKSYAQGQATDIQIRAREVLVNKGSMVDIFARTTGQAPEKI
CCCCCCCCCCCCCCEEEECCHHHCCCCCCEEEEEEEEHHHCCCCEEEEEECCCCCCHHHH
TKDMDRLLYMTPYQAKDYGLIDRVFEKEELANPPLPASVL
HHHHHHHEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MDISPIKAVQAPYYGDSSYRTPPPDLPSLLLKERIVYLGMPLVPAVTELIVAQLLYLQSD
CCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
DPDKPIKIYINSTGTSGYSGEPIGFETEAFAIYDTMKYIKPPIHTICIGSAMGMAAMLLS
CCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AGTKGCRASLPSSSIILHQPKSYAQGQATDIQIRAREVLVNKGSMVDIFARTTGQAPEKI
CCCCCCCCCCCCCCEEEECCHHHCCCCCCEEEEEEEEHHHCCCCEEEEEECCCCCCHHHH
TKDMDRLLYMTPYQAKDYGLIDRVFEKEELANPPLPASVL
HHHHHHHEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP imidodiphosphate; dATP degradation, [C]

Metal ions: Ca2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): 800 [C]

Specific activity: NA

Km value (mM): 1.3 {N-succinyl-Leu-Tyr} 0.21 {ATP}} [C]

Substrates: NA

Specific reaction: NA

General reaction: Hydrolysis of peptide bonds [C]

Inhibitor: Diisopropyl fluorophosphate ClpAP; Fluorosulfonyl benzoyl adenosine; Kappa-Casein; Mg2+; NEM hydrolysis; Neohydrin; Succinyl -Leu-Tyr4-methyl coumarin7-amide; Xaa-Tyr-Leu-Tyr-Trp [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]