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The map label for this gene is dfrA [H]

Identifier: 17231760

GI number: 17231760

Start: 5118257

End: 5119240

Strand: Direct

Name: dfrA [H]

Synonym: alr4268

Alternate gene names: 17231760

Gene position: 5118257-5119240 (Clockwise)

Preceding gene: 17231759

Following gene: 17231761

Centisome position: 79.8

GC content: 47.36

Gene sequence:

>984_bases
ATGCGGGTCTTTGTCACGGGGGCTACGGGTTTTGTTGGTGCTAATCTAGTGCGATTGCTGCTGCAACAAGGATACACGGT
CAAAACCCTAGTCCGTCCTCAGAGCAATCTGGGTAATTTACGCGGTTTAGATGTGGAAATTGTCGAAGGAGATTTCGATA
ATCAGTTCCTTTGGCAACAGATGTCTGGTTGTCGCTATCTGTTTCATGTCGCAGCCCAATACTCCCTGTGGCAAAAAGAC
CGTGATTTACTCTATCAAAACAATGTCCTCGGAACTTTTCAGGTGTTAGAAGCAGCCCAAAAAGCTGGAATTGAACGTAC
TGTTTACACCAGTTCCGTAGCCGCTATTGGGGTCAACCCCTCTGGTGAAATTGCAGATGAAACTTATCAAAGTCCCGTAG
ATAAGCTAATTGGACATTACAAAAAGTCCAAGTTTCTTGCAGAACAGGAAGCTGTACAAGCCGCAGCCAAGGGACAAGAT
ATCGTCATCGTTAACCCCAGCACCCCCATCGGCCCCTGGGATATCAAACCTACACCCACTGGCGATATTATTCTGCGGTT
CCTGCGGCGACAAATGCCCGCTTATGTGAATACAGGGCTGAATTTAATCGATGTGCGGGATGTAGCTTGGGGACATTTAC
TAGCCTTGGAAAAAGGCAAAAGTGGCGATCGCTATATCTTAGGTCATCAAAACCTCAGCCTCCAGCAACTACTTGAGAAA
CTGGCAGAAATCACAGGTTTACCCGCACCCCAATGGACTGTCCCAGGCTGGTTACCCCTAAGCGTCGCCTGGATGGAAGA
AAAGATTCTCGCACCCTTGGGAAAAACTCCCTCAGTCCCTATAGATGGTGTCCGTATGGCACAACAAACCATGTACTACG
ATGCCTCAAAAGCCGTCAAAGAATTAGGTTTACCCCAATCTCCTGTCGATATTGCCTTGAAAGATGCGGTGAATTGGTTT
GTTTCCCAAGGTTATGTCAAGTGA

Upstream 100 bases:

>100_bases
GACTGGTAACTTCTACCATCCTCAGTTTGATTGTGATACCCGTGGTATACGCCATTTTGGACGATTGGTTTCCCCGGTTT
GCCCACAAGGAGAGAAACTA

Downstream 100 bases:

>100_bases
GGTGAAAAAGAGGCAGGGAGAAGAAAACTATTAGCAACTGACAACTGACAACTAACAACTGACTAATGACTATTGAGGAG
TAATACAGTGGCGGTTAACT

Product: hypothetical protein

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MRVFVTGATGFVGANLVRLLLQQGYTVKTLVRPQSNLGNLRGLDVEIVEGDFDNQFLWQQMSGCRYLFHVAAQYSLWQKD
RDLLYQNNVLGTFQVLEAAQKAGIERTVYTSSVAAIGVNPSGEIADETYQSPVDKLIGHYKKSKFLAEQEAVQAAAKGQD
IVIVNPSTPIGPWDIKPTPTGDIILRFLRRQMPAYVNTGLNLIDVRDVAWGHLLALEKGKSGDRYILGHQNLSLQQLLEK
LAEITGLPAPQWTVPGWLPLSVAWMEEKILAPLGKTPSVPIDGVRMAQQTMYYDASKAVKELGLPQSPVDIALKDAVNWF
VSQGYVK

Sequences:

>Translated_327_residues
MRVFVTGATGFVGANLVRLLLQQGYTVKTLVRPQSNLGNLRGLDVEIVEGDFDNQFLWQQMSGCRYLFHVAAQYSLWQKD
RDLLYQNNVLGTFQVLEAAQKAGIERTVYTSSVAAIGVNPSGEIADETYQSPVDKLIGHYKKSKFLAEQEAVQAAAKGQD
IVIVNPSTPIGPWDIKPTPTGDIILRFLRRQMPAYVNTGLNLIDVRDVAWGHLLALEKGKSGDRYILGHQNLSLQQLLEK
LAEITGLPAPQWTVPGWLPLSVAWMEEKILAPLGKTPSVPIDGVRMAQQTMYYDASKAVKELGLPQSPVDIALKDAVNWF
VSQGYVK
>Mature_327_residues
MRVFVTGATGFVGANLVRLLLQQGYTVKTLVRPQSNLGNLRGLDVEIVEGDFDNQFLWQQMSGCRYLFHVAAQYSLWQKD
RDLLYQNNVLGTFQVLEAAQKAGIERTVYTSSVAAIGVNPSGEIADETYQSPVDKLIGHYKKSKFLAEQEAVQAAAKGQD
IVIVNPSTPIGPWDIKPTPTGDIILRFLRRQMPAYVNTGLNLIDVRDVAWGHLLALEKGKSGDRYILGHQNLSLQQLLEK
LAEITGLPAPQWTVPGWLPLSVAWMEEKILAPLGKTPSVPIDGVRMAQQTMYYDASKAVKELGLPQSPVDIALKDAVNWF
VSQGYVK

Specific function: Galactose metabolism; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=344, Percent_Identity=26.1627906976744, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI8393516, Length=344, Percent_Identity=26.1627906976744, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI116268111, Length=354, Percent_Identity=24.5762711864407, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI239745448, Length=349, Percent_Identity=26.0744985673352, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI310132178, Length=349, Percent_Identity=26.0744985673352, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI310113012, Length=349, Percent_Identity=26.0744985673352, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1786974, Length=265, Percent_Identity=26.7924528301887, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71987463, Length=348, Percent_Identity=27.2988505747126, Blast_Score=113, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 36192; Mature: 36192

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVFVTGATGFVGANLVRLLLQQGYTVKTLVRPQSNLGNLRGLDVEIVEGDFDNQFLWQQ
CEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHH
MSGCRYLFHVAAQYSLWQKDRDLLYQNNVLGTFQVLEAAQKAGIERTVYTSSVAAIGVNP
HHCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCEEEEECCC
SGEIADETYQSPVDKLIGHYKKSKFLAEQEAVQAAAKGQDIVIVNPSTPIGPWDIKPTPT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCC
GDIILRFLRRQMPAYVNTGLNLIDVRDVAWGHLLALEKGKSGDRYILGHQNLSLQQLLEK
HHHHHHHHHHHCCHHHHCCCCEEEECHHCHHHEEEEECCCCCCEEEEECCCCCHHHHHHH
LAEITGLPAPQWTVPGWLPLSVAWMEEKILAPLGKTPSVPIDGVRMAQQTMYYDASKAVK
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ELGLPQSPVDIALKDAVNWFVSQGYVK
HCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRVFVTGATGFVGANLVRLLLQQGYTVKTLVRPQSNLGNLRGLDVEIVEGDFDNQFLWQQ
CEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHH
MSGCRYLFHVAAQYSLWQKDRDLLYQNNVLGTFQVLEAAQKAGIERTVYTSSVAAIGVNP
HHCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCEEEEECCC
SGEIADETYQSPVDKLIGHYKKSKFLAEQEAVQAAAKGQDIVIVNPSTPIGPWDIKPTPT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCC
GDIILRFLRRQMPAYVNTGLNLIDVRDVAWGHLLALEKGKSGDRYILGHQNLSLQQLLEK
HHHHHHHHHHHCCHHHHCCCCEEEECHHCHHHEEEEECCCCCCEEEEECCCCCHHHHHHH
LAEITGLPAPQWTVPGWLPLSVAWMEEKILAPLGKTPSVPIDGVRMAQQTMYYDASKAVK
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ELGLPQSPVDIALKDAVNWFVSQGYVK
HCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]