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The map label for this gene is sigF [H]

Identifier: 17231741

GI number: 17231741

Start: 5097964

End: 5099136

Strand: Direct

Name: sigF [H]

Synonym: alr4249

Alternate gene names: 17231741

Gene position: 5097964-5099136 (Clockwise)

Preceding gene: 17231739

Following gene: 17231742

Centisome position: 79.48

GC content: 42.11

Gene sequence:

>1173_bases
ATGTACCAAACAAAGCATGAATCCCTCAAGGAAACTATGAATATTGCTGAATTGGGAACAATGGAAATAATAGAGAGCGT
TGTAGAACATGAAGAACACTCTCTTGATACTTTAGAACTGATAATAGAAGAAGAACCCCCAATAACAGAGAATTTAGACT
CAGAGGAACGTGATAGCGGGGATGAAATGGCGGCTGCCCGACCTTCGGGCTACAATAAAACCGAGCATGATGATGCGGTA
GGTGCGTTCTTTAAAGAAATGGCACGTTATCCACTGCTAAAACCCGATGAAGAGGTAGAATTAGCCAGAAGAGTCAGATT
TTTAGAAGAAGTTAGAGATTTACAAGCAGATCTGCAATTACAAATAGGGCAACATCCGACAAAAGCCCAAGTAGCTGGTC
AACTAGAGATAACAGAAAAACAATTAGAAAATCGATTGTATCAAGGTCGAGTGGCGAAGCGTAAAATGATTCGCTCGAAC
TTGAGATTAGTAGTATCAATTGCCAAGCGATATCTAAATCGGGGAGTACCTTTTCTAGATTTAATTCAAGAAGGTGCAAT
GGGATTGAATCGAGCTACAGAAAAATTTGATCCCGATAAAGGATATAAGTTCTCTACTTATGCTTACTGGTGGATTAGAC
AAGCGATTACGAGAGCGATCGCTAATGATGCCAGAACCATCCGCCTACCCATCCATATTGTAGAAAAACTCAACAAACTC
AAAAAAGCCCAACGGGAACTCAAACAAAAATTCAGCCGCAACCCTTCCGAAGCAGAAATGGCCGAAGCTTTGGAAATTAG
CGTCCCCCAACTGCGCCAACTGCAACAACTCCGGCGACAAGCCCTATCTTTAAACCATCGTGTCGGTAAAGAAGAAGATA
CCGAACTGATGGATTTATTAGAAGATGAGGATAACTTATCTCCGGAAGCAAAAATGAACGAAAATATGATGCGTCAGGAG
ATTTGGGAAGTTTTAGGTGATGTCCTCACTCCCAGAGAAAAAGACGTAATTTCTCTACGCTATGGTTTGACAACTAGCGA
ACCTTGCACCCTGGAGGAAGTCGGAAATATGTTCAACTTATCCCGCGAACGAGTCCGCCAAATTCAAAGCAAAGCCATGC
GTAAATTACGCCGTCCTCACATAGCCAAACGTCTTAAGGGTTGGTTGATTTGA

Upstream 100 bases:

>100_bases
CTAGAAATTAATTACATGAATCTGAAAATTGCTACTTAAAAGACACAATCTTAGTTAGGAAGCCCAACAATTCTCAGTAG
TTAACAGTTGTCATGAAATT

Downstream 100 bases:

>100_bases
GCTAGGAATTAGGGATTAGAGATTGGACTTTAGTATTTTTACTTAGGACTCATAACTAATACCAATTTGAAAAAGGAATG
CGACAGATATAAAGCCCCAA

Product: group 2 sigma 70-type sigma factor F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MYQTKHESLKETMNIAELGTMEIIESVVEHEEHSLDTLELIIEEEPPITENLDSEERDSGDEMAAARPSGYNKTEHDDAV
GAFFKEMARYPLLKPDEEVELARRVRFLEEVRDLQADLQLQIGQHPTKAQVAGQLEITEKQLENRLYQGRVAKRKMIRSN
LRLVVSIAKRYLNRGVPFLDLIQEGAMGLNRATEKFDPDKGYKFSTYAYWWIRQAITRAIANDARTIRLPIHIVEKLNKL
KKAQRELKQKFSRNPSEAEMAEALEISVPQLRQLQQLRRQALSLNHRVGKEEDTELMDLLEDEDNLSPEAKMNENMMRQE
IWEVLGDVLTPREKDVISLRYGLTTSEPCTLEEVGNMFNLSRERVRQIQSKAMRKLRRPHIAKRLKGWLI

Sequences:

>Translated_390_residues
MYQTKHESLKETMNIAELGTMEIIESVVEHEEHSLDTLELIIEEEPPITENLDSEERDSGDEMAAARPSGYNKTEHDDAV
GAFFKEMARYPLLKPDEEVELARRVRFLEEVRDLQADLQLQIGQHPTKAQVAGQLEITEKQLENRLYQGRVAKRKMIRSN
LRLVVSIAKRYLNRGVPFLDLIQEGAMGLNRATEKFDPDKGYKFSTYAYWWIRQAITRAIANDARTIRLPIHIVEKLNKL
KKAQRELKQKFSRNPSEAEMAEALEISVPQLRQLQQLRRQALSLNHRVGKEEDTELMDLLEDEDNLSPEAKMNENMMRQE
IWEVLGDVLTPREKDVISLRYGLTTSEPCTLEEVGNMFNLSRERVRQIQSKAMRKLRRPHIAKRLKGWLI
>Mature_390_residues
MYQTKHESLKETMNIAELGTMEIIESVVEHEEHSLDTLELIIEEEPPITENLDSEERDSGDEMAAARPSGYNKTEHDDAV
GAFFKEMARYPLLKPDEEVELARRVRFLEEVRDLQADLQLQIGQHPTKAQVAGQLEITEKQLENRLYQGRVAKRKMIRSN
LRLVVSIAKRYLNRGVPFLDLIQEGAMGLNRATEKFDPDKGYKFSTYAYWWIRQAITRAIANDARTIRLPIHIVEKLNKL
KKAQRELKQKFSRNPSEAEMAEALEISVPQLRQLQQLRRQALSLNHRVGKEEDTELMDLLEDEDNLSPEAKMNENMMRQE
IWEVLGDVLTPREKDVISLRYGLTTSEPCTLEEVGNMFNLSRERVRQIQSKAMRKLRRPHIAKRLKGWLI

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is a component of the biological clock pathway that affects the circadian expression of a subset of ge

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=242, Percent_Identity=45.0413223140496, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1789098, Length=300, Percent_Identity=40.6666666666667, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1789871, Length=250, Percent_Identity=30, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788231, Length=204, Percent_Identity=28.921568627451, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 45371; Mature: 45371

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQTKHESLKETMNIAELGTMEIIESVVEHEEHSLDTLELIIEEEPPITENLDSEERDSG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCHHCCCC
DEMAAARPSGYNKTEHDDAVGAFFKEMARYPLLKPDEEVELARRVRFLEEVRDLQADLQL
CHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QIGQHPTKAQVAGQLEITEKQLENRLYQGRVAKRKMIRSNLRLVVSIAKRYLNRGVPFLD
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LIQEGAMGLNRATEKFDPDKGYKFSTYAYWWIRQAITRAIANDARTIRLPIHIVEKLNKL
HHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
KKAQRELKQKFSRNPSEAEMAEALEISVPQLRQLQQLRRQALSLNHRVGKEEDTELMDLL
HHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
EDEDNLSPEAKMNENMMRQEIWEVLGDVLTPREKDVISLRYGLTTSEPCTLEEVGNMFNL
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHH
SRERVRQIQSKAMRKLRRPHIAKRLKGWLI
HHHHHHHHHHHHHHHHHCHHHHHHHHCCCC
>Mature Secondary Structure
MYQTKHESLKETMNIAELGTMEIIESVVEHEEHSLDTLELIIEEEPPITENLDSEERDSG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCHHCCCC
DEMAAARPSGYNKTEHDDAVGAFFKEMARYPLLKPDEEVELARRVRFLEEVRDLQADLQL
CHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QIGQHPTKAQVAGQLEITEKQLENRLYQGRVAKRKMIRSNLRLVVSIAKRYLNRGVPFLD
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LIQEGAMGLNRATEKFDPDKGYKFSTYAYWWIRQAITRAIANDARTIRLPIHIVEKLNKL
HHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
KKAQRELKQKFSRNPSEAEMAEALEISVPQLRQLQQLRRQALSLNHRVGKEEDTELMDLL
HHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
EDEDNLSPEAKMNENMMRQEIWEVLGDVLTPREKDVISLRYGLTTSEPCTLEEVGNMFNL
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHH
SRERVRQIQSKAMRKLRRPHIAKRLKGWLI
HHHHHHHHHHHHHHHHHCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8665856; 1368828 [H]