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The map label for this gene is truA

Identifier: 17231681

GI number: 17231681

Start: 5037043

End: 5037891

Strand: Reverse

Name: truA

Synonym: all4189

Alternate gene names: 17231681

Gene position: 5037891-5037043 (Counterclockwise)

Preceding gene: 17231682

Following gene: 17231680

Centisome position: 78.55

GC content: 47.35

Gene sequence:

>849_bases
ATGTTAGATAGTCACCAGCCGAAACAAACTCAACGAGTAGCCTTGGTAGTGCAGTACCTTGGTACACATTTTCATGGCTG
GCAACGACAAAAACAGCATCGGACAGTTCAGGAAGAAATCGAAACAGCGATCGCTAAAATTCTCGGACATCATGTCACAC
TACATGGTGCTGGGCGTACTGATAGCGGAGTCCACGCTGCTGCTCAAGTAGCCCATTTTGATGCTACAGGGTTAATTCCC
GCACACAAGTGGGCATCAGTTATCAATAGTTACCTGCCCAAGGATATATTGATAAAAGCTTCAGCAGCTGTAGGCGACCG
TTGGCACGCTCGTTTTAGCGCCGCATACAGGCGGTATCGCTACACAATCTATACTGAAGATCGACCGAACTTGTTCGTCA
CACCTTTCAGTTGGCATTATTATTATGCACCCCTAGATGAATCTCTGATGCAGGCTGCCCTTAAACCCCTGATGGGAAAG
CATCACTTGGCGGCATTTCACCGTGCAGGGTCAAAGCGATCGCATTCTTGGGTAGAGGTACAAGCAGTAGAGTGTCATCG
TAGTGGGCCGTTTATCCATATTGAAATTCAGGCTGATGGATTTTTATATGGCATGGTGCGACTGTTGGTAGGGATGTTAG
TACAGGTAGGTTCCGGGCAACGAACTCTGGCTAACTTCACCGAACTCTGGAAAGAACAACGGCGGGAAGAAGTAAAACAC
GCCGCACCGTCACAGGGCTTATGCTTGTTACGAGTCGGCTATCCAGATTTTCCCTTTACCCCTGACGTTTGGTATGACAC
CATGCCAAAGTTAGTTTTTAGTCAAGAGTCATTAGTCAATAGTCATTAG

Upstream 100 bases:

>100_bases
TATTGAATTGGTGTAGAGAAGACAGGAGGTAGAGGGCTGGAGGCAAAAGGGTAAATCCTTTGCTTAAAACCTTGTACTTC
AGTCTTTATTGAAAGAACCT

Downstream 100 bases:

>100_bases
TCAAAAGTTTTTGCTATGGACTACAGACTATTGACTATCAGACTCTGAAAAAAATGACAAAGGACAAATAAAAATGAGTA
AAACCTATCTTCCTCCTCAA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MLDSHQPKQTQRVALVVQYLGTHFHGWQRQKQHRTVQEEIETAIAKILGHHVTLHGAGRTDSGVHAAAQVAHFDATGLIP
AHKWASVINSYLPKDILIKASAAVGDRWHARFSAAYRRYRYTIYTEDRPNLFVTPFSWHYYYAPLDESLMQAALKPLMGK
HHLAAFHRAGSKRSHSWVEVQAVECHRSGPFIHIEIQADGFLYGMVRLLVGMLVQVGSGQRTLANFTELWKEQRREEVKH
AAPSQGLCLLRVGYPDFPFTPDVWYDTMPKLVFSQESLVNSH

Sequences:

>Translated_282_residues
MLDSHQPKQTQRVALVVQYLGTHFHGWQRQKQHRTVQEEIETAIAKILGHHVTLHGAGRTDSGVHAAAQVAHFDATGLIP
AHKWASVINSYLPKDILIKASAAVGDRWHARFSAAYRRYRYTIYTEDRPNLFVTPFSWHYYYAPLDESLMQAALKPLMGK
HHLAAFHRAGSKRSHSWVEVQAVECHRSGPFIHIEIQADGFLYGMVRLLVGMLVQVGSGQRTLANFTELWKEQRREEVKH
AAPSQGLCLLRVGYPDFPFTPDVWYDTMPKLVFSQESLVNSH
>Mature_282_residues
MLDSHQPKQTQRVALVVQYLGTHFHGWQRQKQHRTVQEEIETAIAKILGHHVTLHGAGRTDSGVHAAAQVAHFDATGLIP
AHKWASVINSYLPKDILIKASAAVGDRWHARFSAAYRRYRYTIYTEDRPNLFVTPFSWHYYYAPLDESLMQAALKPLMGK
HHLAAFHRAGSKRSHSWVEVQAVECHRSGPFIHIEIQADGFLYGMVRLLVGMLVQVGSGQRTLANFTELWKEQRREEVKH
AAPSQGLCLLRVGYPDFPFTPDVWYDTMPKLVFSQESLVNSH

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=270, Percent_Identity=29.6296296296296, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1788657, Length=257, Percent_Identity=36.1867704280156, Blast_Score=175, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_NOSS1 (Q8YPK5)

Other databases:

- EMBL:   BA000019
- PIR:   AF2329
- RefSeq:   NP_488229.1
- ProteinModelPortal:   Q8YPK5
- SMR:   Q8YPK5
- STRING:   Q8YPK5
- GeneID:   1107791
- GenomeReviews:   BA000019_GR
- KEGG:   ana:all4189
- NMPDR:   fig|103690.1.peg.4496
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   HSAIRRH
- PhylomeDB:   Q8YPK5
- ProtClustDB:   PRK00021
- BioCyc:   NSP103690:ALL4189-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 32141; Mature: 32141

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: ACT_SITE 61-61

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDSHQPKQTQRVALVVQYLGTHFHGWQRQKQHRTVQEEIETAIAKILGHHVTLHGAGRT
CCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCC
DSGVHAAAQVAHFDATGLIPAHKWASVINSYLPKDILIKASAAVGDRWHARFSAAYRRYR
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHEEEEHHHHCHHHHHHHHHHHHHEE
YTIYTEDRPNLFVTPFSWHYYYAPLDESLMQAALKPLMGKHHLAAFHRAGSKRSHSWVEV
EEEEECCCCCEEEEECCCEEEECCHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCEEEE
QAVECHRSGPFIHIEIQADGFLYGMVRLLVGMLVQVGSGQRTLANFTELWKEQRREEVKH
EEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AAPSQGLCLLRVGYPDFPFTPDVWYDTMPKLVFSQESLVNSH
CCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDSHQPKQTQRVALVVQYLGTHFHGWQRQKQHRTVQEEIETAIAKILGHHVTLHGAGRT
CCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCC
DSGVHAAAQVAHFDATGLIPAHKWASVINSYLPKDILIKASAAVGDRWHARFSAAYRRYR
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHEEEEHHHHCHHHHHHHHHHHHHEE
YTIYTEDRPNLFVTPFSWHYYYAPLDESLMQAALKPLMGKHHLAAFHRAGSKRSHSWVEV
EEEEECCCCCEEEEECCCEEEECCHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCEEEE
QAVECHRSGPFIHIEIQADGFLYGMVRLLVGMLVQVGSGQRTLANFTELWKEQRREEVKH
EEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AAPSQGLCLLRVGYPDFPFTPDVWYDTMPKLVFSQESLVNSH
CCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840