Click here to switch to the map view.

The map label for this gene is barA [H]

Identifier: 17231597

GI number: 17231597

Start: 4946264

End: 4947916

Strand: Direct

Name: barA [H]

Synonym: alr4105

Alternate gene names: 17231597

Gene position: 4946264-4947916 (Clockwise)

Preceding gene: 17231596

Following gene: 17231600

Centisome position: 77.12

GC content: 37.51

Gene sequence:

>1653_bases
GTGGAACTGAGAAGCATCGCTCCCCCAAAACCTGGAGTAACAGGCCCACCTAATCCTCAAGAGCAATTACAATTGCTACT
CACAGCCCCCGTATATGATAATTCCGGGAAATTGCAATATGCTTTAAATCTTCAGTCAGCATTGTATAAACAAACTCGTA
ATCAACCAGGTTCACTCACTGGTTCCACCATAGTCATTGCTGAAGATGGGACGATATTAGCTCATCCATTACCTGATTTA
GTGGGAACTAATATCAAAGAACACTCAGATGCAAAGCAAGTACAAAGCATCATCAAAAATGCAATTGCAGGACGTAATGA
GCCAATCAATTTAATTTTCAATAATAAAAAAGAATTAGTAGCAGGTTATACAGTAATTGACAGTCCCACAAGCATAAAGC
CACAGCAGAAATGGATAGTCATGTCTGTGACAACTGTAGAAAATGCTCTATTTGGCTTAGAAGAAATCAAACTCATCCTA
ATTGTTTTAACAGTTGGTTTAATTGGTGCAAGTTTAGTAGCATCTTTGTATCTAGCGCCTTACCTAGCTAGCCCTGTGGA
AGAATTGCGCGATTATGCTTTAAACATTCATAGCCACCACACCTCTAGACGAGTCCCTGATAACTTCAAGATTCGCGAGT
TTAACCAACTAGCCCAAGCAATAGACCAAATGGTTGATAGGCTTAAAGTTTGGTCAGAAGAAATAGAGACTGCATGGAAA
GACGCAAAAAGTGCTAATCAAGTTAAAAGTCAGTTTTTAGCTACCACTTCTCACGAATTACGCAACCCATTAAATATTAT
TATCAACTGCGTACGTTTAGTTAAAGATGGTTTGTGTGATAATCGAGAGGAAGAAATAGAATTTCTTCAGCGTGCTGATG
AAACAGCTATACATTTACTAGGAATTATTAACGAACTACTCGATATTTCTAAGATTGAAGCTGGGAAACTTTCTGTAGTT
AGCCAACCTTTAGATTTACGACAAACACTTTTAGAAGTAATCAATTTACAGTCAGTTAATGTTCAACAAAAAGGCCTGCA
ACTGAAAACCAACTTAGGCACAGAACCCATTCCCGTCAAGGCGGATGCAGCTAAATTAAAACAGGTATTAATTAATATAA
TTGGTAATGCTACTAAATTCACCGATGAAGGTGGTATTAGTATTGCGACAACCATTCAAACTCGAATTGAGGGTAAATCG
CAAGTGATGGTTAGTATTACAGATACCGGCTTAGGGATTGAACCTGCTCAACAACATAAACTATTCCGTCCCTTTGTGAT
GGTGAATGGTAGCACTACACGAAAGTTTGAGGGTACAGGCTTGGGTTTAGCCATTTCACGAAACTTAATTGAACTCATGG
GTGGTACTATCACTTTGGAGAGTGCTGGTATAAATCAAGGTACGACAGTTAATATTACTTTGCCATTGATTGATATCTCT
TTATTAGGTTTATCAGCCACAGATAATAATATTAAAAACCAGAAAAATTTAGAAGAAGATGAGGAAATTAAGGGAACTGA
TTCTTTCTCCTCTAACCATCCAGAAGCAATACCTCTAGAGTGTAATGAAAACTACGGCTCTAAAAAAACAAAGTTAGAAG
AAGAGTATATTGATCTAGAGCGGGTTAATCTTTTTATGATGCGTAATTCGTAA

Upstream 100 bases:

>100_bases
ATGCTTACAACTAATTAATTGGCAAAATGGTGAAATCGTTGCCAGTAGCTGCGGTAAAAAAGAAATTACTCAATTTGGCC
CATTTCTACCAACTGATAGT

Downstream 100 bases:

>100_bases
TTGATTTTGTGATGTGGATAAGCTTTCGTATTGTAGTAGTTGTGTAAATTATTATAAATTTATTTAAGAGGCACGGATTG
ACCGCCCTGTTGGAGAGATG

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MELRSIAPPKPGVTGPPNPQEQLQLLLTAPVYDNSGKLQYALNLQSALYKQTRNQPGSLTGSTIVIAEDGTILAHPLPDL
VGTNIKEHSDAKQVQSIIKNAIAGRNEPINLIFNNKKELVAGYTVIDSPTSIKPQQKWIVMSVTTVENALFGLEEIKLIL
IVLTVGLIGASLVASLYLAPYLASPVEELRDYALNIHSHHTSRRVPDNFKIREFNQLAQAIDQMVDRLKVWSEEIETAWK
DAKSANQVKSQFLATTSHELRNPLNIIINCVRLVKDGLCDNREEEIEFLQRADETAIHLLGIINELLDISKIEAGKLSVV
SQPLDLRQTLLEVINLQSVNVQQKGLQLKTNLGTEPIPVKADAAKLKQVLINIIGNATKFTDEGGISIATTIQTRIEGKS
QVMVSITDTGLGIEPAQQHKLFRPFVMVNGSTTRKFEGTGLGLAISRNLIELMGGTITLESAGINQGTTVNITLPLIDIS
LLGLSATDNNIKNQKNLEEDEEIKGTDSFSSNHPEAIPLECNENYGSKKTKLEEEYIDLERVNLFMMRNS

Sequences:

>Translated_550_residues
MELRSIAPPKPGVTGPPNPQEQLQLLLTAPVYDNSGKLQYALNLQSALYKQTRNQPGSLTGSTIVIAEDGTILAHPLPDL
VGTNIKEHSDAKQVQSIIKNAIAGRNEPINLIFNNKKELVAGYTVIDSPTSIKPQQKWIVMSVTTVENALFGLEEIKLIL
IVLTVGLIGASLVASLYLAPYLASPVEELRDYALNIHSHHTSRRVPDNFKIREFNQLAQAIDQMVDRLKVWSEEIETAWK
DAKSANQVKSQFLATTSHELRNPLNIIINCVRLVKDGLCDNREEEIEFLQRADETAIHLLGIINELLDISKIEAGKLSVV
SQPLDLRQTLLEVINLQSVNVQQKGLQLKTNLGTEPIPVKADAAKLKQVLINIIGNATKFTDEGGISIATTIQTRIEGKS
QVMVSITDTGLGIEPAQQHKLFRPFVMVNGSTTRKFEGTGLGLAISRNLIELMGGTITLESAGINQGTTVNITLPLIDIS
LLGLSATDNNIKNQKNLEEDEEIKGTDSFSSNHPEAIPLECNENYGSKKTKLEEEYIDLERVNLFMMRNS
>Mature_550_residues
MELRSIAPPKPGVTGPPNPQEQLQLLLTAPVYDNSGKLQYALNLQSALYKQTRNQPGSLTGSTIVIAEDGTILAHPLPDL
VGTNIKEHSDAKQVQSIIKNAIAGRNEPINLIFNNKKELVAGYTVIDSPTSIKPQQKWIVMSVTTVENALFGLEEIKLIL
IVLTVGLIGASLVASLYLAPYLASPVEELRDYALNIHSHHTSRRVPDNFKIREFNQLAQAIDQMVDRLKVWSEEIETAWK
DAKSANQVKSQFLATTSHELRNPLNIIINCVRLVKDGLCDNREEEIEFLQRADETAIHLLGIINELLDISKIEAGKLSVV
SQPLDLRQTLLEVINLQSVNVQQKGLQLKTNLGTEPIPVKADAAKLKQVLINIIGNATKFTDEGGISIATTIQTRIEGKS
QVMVSITDTGLGIEPAQQHKLFRPFVMVNGSTTRKFEGTGLGLAISRNLIELMGGTITLESAGINQGTTVNITLPLIDIS
LLGLSATDNNIKNQKNLEEDEEIKGTDSFSSNHPEAIPLECNENYGSKKTKLEEEYIDLERVNLFMMRNS

Specific function: Member of the two-component regulatory system UvrY/BarA involved in the regulation of carbon metabolism via the CsrA/CsrB regulatory system. Phosphorylates UvrY, probably via a four-step phosphorelay [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=346, Percent_Identity=34.393063583815, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI87081816, Length=287, Percent_Identity=35.191637630662, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI48994928, Length=240, Percent_Identity=34.5833333333333, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI1788713, Length=237, Percent_Identity=35.4430379746835, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI145693157, Length=264, Percent_Identity=35.2272727272727, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788393, Length=296, Percent_Identity=28.3783783783784, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1786912, Length=242, Percent_Identity=29.3388429752066, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1790346, Length=238, Percent_Identity=29.4117647058824, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1788549, Length=226, Percent_Identity=32.3008849557522, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1790436, Length=223, Percent_Identity=30.0448430493274, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1786600, Length=239, Percent_Identity=25.1046025104602, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1786783, Length=319, Percent_Identity=25.3918495297806, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87082128, Length=231, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789808, Length=268, Percent_Identity=25, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=75, Percent_Identity=42.6666666666667, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR019247
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF09984 DUF2222; PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 60550; Mature: 60550

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRSIAPPKPGVTGPPNPQEQLQLLLTAPVYDNSGKLQYALNLQSALYKQTRNQPGSLT
CCCCCCCCCCCCCCCCCCHHHHEEEEEECCEECCCCCEEEEECHHHHHHHHHCCCCCCCC
GSTIVIAEDGTILAHPLPDLVGTNIKEHSDAKQVQSIIKNAIAGRNEPINLIFNNKKELV
CCEEEEECCCEEEECCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCEE
AGYTVIDSPTSIKPQQKWIVMSVTTVENALFGLEEIKLILIVLTVGLIGASLVASLYLAP
EEEEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLASPVEELRDYALNIHSHHTSRRVPDNFKIREFNQLAQAIDQMVDRLKVWSEEIETAWK
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAKSANQVKSQFLATTSHELRNPLNIIINCVRLVKDGLCDNREEEIEFLQRADETAIHLL
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
GIINELLDISKIEAGKLSVVSQPLDLRQTLLEVINLQSVNVQQKGLQLKTNLGTEPIPVK
HHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCCCHHHCCCEEEECCCCCCCCCC
ADAAKLKQVLINIIGNATKFTDEGGISIATTIQTRIEGKSQVMVSITDTGLGIEPAQQHK
CCHHHHHHHHHHHHCCCCEECCCCCEEEEEEEEECCCCCCEEEEEEECCCCCCCCHHHHH
LFRPFVMVNGSTTRKFEGTGLGLAISRNLIELMGGTITLESAGINQGTTVNITLPLIDIS
CCCCEEEECCCCCEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEEEEEEE
LLGLSATDNNIKNQKNLEEDEEIKGTDSFSSNHPEAIPLECNENYGSKKTKLEEEYIDLE
EEEEECCCCCCCCCCCCCCHHHCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCHH
RVNLFMMRNS
HEEEEEEECC
>Mature Secondary Structure
MELRSIAPPKPGVTGPPNPQEQLQLLLTAPVYDNSGKLQYALNLQSALYKQTRNQPGSLT
CCCCCCCCCCCCCCCCCCHHHHEEEEEECCEECCCCCEEEEECHHHHHHHHHCCCCCCCC
GSTIVIAEDGTILAHPLPDLVGTNIKEHSDAKQVQSIIKNAIAGRNEPINLIFNNKKELV
CCEEEEECCCEEEECCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCEE
AGYTVIDSPTSIKPQQKWIVMSVTTVENALFGLEEIKLILIVLTVGLIGASLVASLYLAP
EEEEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLASPVEELRDYALNIHSHHTSRRVPDNFKIREFNQLAQAIDQMVDRLKVWSEEIETAWK
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAKSANQVKSQFLATTSHELRNPLNIIINCVRLVKDGLCDNREEEIEFLQRADETAIHLL
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
GIINELLDISKIEAGKLSVVSQPLDLRQTLLEVINLQSVNVQQKGLQLKTNLGTEPIPVK
HHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCCCHHHCCCEEEECCCCCCCCCC
ADAAKLKQVLINIIGNATKFTDEGGISIATTIQTRIEGKSQVMVSITDTGLGIEPAQQHK
CCHHHHHHHHHHHHCCCCEECCCCCEEEEEEEEECCCCCCEEEEEEECCCCCCCCHHHHH
LFRPFVMVNGSTTRKFEGTGLGLAISRNLIELMGGTITLESAGINQGTTVNITLPLIDIS
CCCCEEEECCCCCEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEEEEEEE
LLGLSATDNNIKNQKNLEEDEEIKGTDSFSSNHPEAIPLECNENYGSKKTKLEEEYIDLE
EEEEECCCCCCCCCCCCCCHHHCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCHH
RVNLFMMRNS
HEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]