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The map label for this gene is fadD21 [H]

Identifier: 17231549

GI number: 17231549

Start: 4883744

End: 4885552

Strand: Direct

Name: fadD21 [H]

Synonym: alr4057

Alternate gene names: 17231549

Gene position: 4883744-4885552 (Clockwise)

Preceding gene: 17231548

Following gene: 17231550

Centisome position: 76.14

GC content: 47.93

Gene sequence:

>1809_bases
ATGACAAACGTCTCTACCCTGGTTGAACTGCTACGCGCTCGTGCTAATCATGAGCCGGACAAGCTCGCCTATATGTTTCT
CATCGATGGGAAAACAGAAGGCCCAAAGTTAACATACGCAGAATTGGATCGTCTAGCCCGTGCAATTGGTGCATTATTGC
AGAAACATAATGCTAAAGGTGAACGTGTTTTGCTGCTGTACCCCCAGGGATTAGACGTGATGGCAGCATTTTTGGGTAGT
CTTTATGGTGGAGTCATTGCCATCCCTGCACCTCCTCCTGATGCTGGTAGATTGAAACGGGCTTTGCCTCGGTTACGGGC
TATTGTTAAGGATGCCAACGCTAAATTTGTCTTCACCAACCAGCACCTTTTGGGTGTTCTGCAAGCAGCCAAGTTAGATT
TTCCCGAATTTGAGGAAATGACTTGGTTTGCCAGCGAAGACATCGATTTAGAACTAGCAGACCAATGGCAAGACCCTGAA
ATTACTCCAAATACCCTGGCATATTTGCAGTACACATCAGGCTCGACTTCCACACCAAAGGGTGTGATGATTAGCCACCA
CAACATCATGCACCACTGCGCTTATCTACAAAAAGCTTGTGGCTATGATACTGAGAGCGTTTCCATCACCTGGATGCCAT
ATTTCCACGATTACGGGCTAGTAGAAGGGTTGACCGTACCCATTTACAACGGTCATCCTTGCTATGTTATGTCCCCTATG
TCCTTTATTAAACAGCCTGTACGTTGGTTACAAGCAATTTCTCGCTATCGCGGTACTCATAGTCAAGCTCCTAACTTTGC
TTATGAACAGTGCATTCGCCGCGTCACCGATGCACAACTAGCTACCCTGGATCTCAGCAGTTGGGTAGCAGCCGGGAATG
CCGCCGAACCAATTAACCCTAGAGTGTTAGAAGAGTTTTTTGAGAAGTTCGCCCCTAGTGGGTTCAAGTGGGAAACCTTT
GCACCCGCTTATGGATTGGCAGAAAATACACTGTTGGTTTCTACCAGCCCCAGAAATACACCACCTGTCCTGTGTTTGGT
AGAAAAAACCAAAATTGAGCAGAACAAAATTGTGGAAGCCACTACCTGGGACGATGGAGTCCGGGCTATCCCTGGATGCG
GTCGCTTAGTTTGTGAGACGCAAGTGGCAATTGTCAATCCAGATACTTTGACTCGTTGTGGGACGGATGAAGTAGGCGAG
GTATGGGTAGCAGACCCTAGCGTGGCTGGTGGTTATTGGCAACGCCCCCAAGAAAGCGAAAGTACCTTCCGTGCCAAGAT
TGCCGGGAATAATCAAGTCTCTTTCCTCCGCACAGGTGACTTGGGCTTTATCAAGGGTGGTGAGTTGTTCATCACCGGAC
GGATCAAAGATTTGATTATTATCCGGGGGACTAACCATTATCCCCAAGACTTGGAATGGACTGTACAGGAAATTCACCCA
GCCCTTCGCCCAGACTATGGTGCAGCCTTCTCCATCGATGTGGATGGAGTGGAACAACTGGTAGTCGTCCAGGAAGTTAA
ACGCAAACCAGAAGAAGAGTTTAACTCAGATGAGGTATTGACCAATATTCGGCAAGCGATCGCCGAAATTCATGAACTGC
AAGCATACGCTGTGGTTCTTGTCAAGCCTGGTAATGTTCTCAAAACCTCCAGTGGCAAAATTCAACGACGTGCCTGCAAA
GCCAGTTTCCTCGCCGGTGAGTTGGAAGTTTTAGCCGATTGGAGCGAAAACCCCAAATACACCGCCAGCTATCGGCGACT
ACAAGGGGAAGTAAATTCTTTGCTGGAAAAGGTGCAAGTTACTCACTAA

Upstream 100 bases:

>100_bases
GCAGCACATCTGCAAAAAGTAAGATATGTGATGCCATAGTTTCCAGATAAGTTTTGGAAACACCAAATGACTCACTGCCT
AACTATCCAAGAAGTTGCCA

Downstream 100 bases:

>100_bases
GGAGAAAATTATGCAGCCACTCAAGCAATATTGGATCGCAGAAGCATTAGAGAAAGATTTGGGCGACCCTAGCAACCCAA
ATAATGTGATGTCGTTTAAG

Product: hypothetical protein

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 602; Mature: 601

Protein sequence:

>602_residues
MTNVSTLVELLRARANHEPDKLAYMFLIDGKTEGPKLTYAELDRLARAIGALLQKHNAKGERVLLLYPQGLDVMAAFLGS
LYGGVIAIPAPPPDAGRLKRALPRLRAIVKDANAKFVFTNQHLLGVLQAAKLDFPEFEEMTWFASEDIDLELADQWQDPE
ITPNTLAYLQYTSGSTSTPKGVMISHHNIMHHCAYLQKACGYDTESVSITWMPYFHDYGLVEGLTVPIYNGHPCYVMSPM
SFIKQPVRWLQAISRYRGTHSQAPNFAYEQCIRRVTDAQLATLDLSSWVAAGNAAEPINPRVLEEFFEKFAPSGFKWETF
APAYGLAENTLLVSTSPRNTPPVLCLVEKTKIEQNKIVEATTWDDGVRAIPGCGRLVCETQVAIVNPDTLTRCGTDEVGE
VWVADPSVAGGYWQRPQESESTFRAKIAGNNQVSFLRTGDLGFIKGGELFITGRIKDLIIIRGTNHYPQDLEWTVQEIHP
ALRPDYGAAFSIDVDGVEQLVVVQEVKRKPEEEFNSDEVLTNIRQAIAEIHELQAYAVVLVKPGNVLKTSSGKIQRRACK
ASFLAGELEVLADWSENPKYTASYRRLQGEVNSLLEKVQVTH

Sequences:

>Translated_602_residues
MTNVSTLVELLRARANHEPDKLAYMFLIDGKTEGPKLTYAELDRLARAIGALLQKHNAKGERVLLLYPQGLDVMAAFLGS
LYGGVIAIPAPPPDAGRLKRALPRLRAIVKDANAKFVFTNQHLLGVLQAAKLDFPEFEEMTWFASEDIDLELADQWQDPE
ITPNTLAYLQYTSGSTSTPKGVMISHHNIMHHCAYLQKACGYDTESVSITWMPYFHDYGLVEGLTVPIYNGHPCYVMSPM
SFIKQPVRWLQAISRYRGTHSQAPNFAYEQCIRRVTDAQLATLDLSSWVAAGNAAEPINPRVLEEFFEKFAPSGFKWETF
APAYGLAENTLLVSTSPRNTPPVLCLVEKTKIEQNKIVEATTWDDGVRAIPGCGRLVCETQVAIVNPDTLTRCGTDEVGE
VWVADPSVAGGYWQRPQESESTFRAKIAGNNQVSFLRTGDLGFIKGGELFITGRIKDLIIIRGTNHYPQDLEWTVQEIHP
ALRPDYGAAFSIDVDGVEQLVVVQEVKRKPEEEFNSDEVLTNIRQAIAEIHELQAYAVVLVKPGNVLKTSSGKIQRRACK
ASFLAGELEVLADWSENPKYTASYRRLQGEVNSLLEKVQVTH
>Mature_601_residues
TNVSTLVELLRARANHEPDKLAYMFLIDGKTEGPKLTYAELDRLARAIGALLQKHNAKGERVLLLYPQGLDVMAAFLGSL
YGGVIAIPAPPPDAGRLKRALPRLRAIVKDANAKFVFTNQHLLGVLQAAKLDFPEFEEMTWFASEDIDLELADQWQDPEI
TPNTLAYLQYTSGSTSTPKGVMISHHNIMHHCAYLQKACGYDTESVSITWMPYFHDYGLVEGLTVPIYNGHPCYVMSPMS
FIKQPVRWLQAISRYRGTHSQAPNFAYEQCIRRVTDAQLATLDLSSWVAAGNAAEPINPRVLEEFFEKFAPSGFKWETFA
PAYGLAENTLLVSTSPRNTPPVLCLVEKTKIEQNKIVEATTWDDGVRAIPGCGRLVCETQVAIVNPDTLTRCGTDEVGEV
WVADPSVAGGYWQRPQESESTFRAKIAGNNQVSFLRTGDLGFIKGGELFITGRIKDLIIIRGTNHYPQDLEWTVQEIHPA
LRPDYGAAFSIDVDGVEQLVVVQEVKRKPEEEFNSDEVLTNIRQAIAEIHELQAYAVVLVKPGNVLKTSSGKIQRRACKA
SFLAGELEVLADWSENPKYTASYRRLQGEVNSLLEKVQVTH

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI55749758, Length=592, Percent_Identity=26.8581081081081, Blast_Score=172, Evalue=7e-43,
Organism=Homo sapiens, GI44888818, Length=594, Percent_Identity=25.9259259259259, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI45827692, Length=595, Percent_Identity=25.3781512605042, Blast_Score=149, Evalue=9e-36,
Organism=Homo sapiens, GI225735629, Length=595, Percent_Identity=25.3781512605042, Blast_Score=149, Evalue=9e-36,
Organism=Homo sapiens, GI225735625, Length=569, Percent_Identity=24.6045694200351, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI45827694, Length=513, Percent_Identity=23.9766081871345, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI45827696, Length=478, Percent_Identity=23.4309623430962, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI225735627, Length=478, Percent_Identity=23.4309623430962, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI156151445, Length=591, Percent_Identity=23.8578680203046, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI45827698, Length=441, Percent_Identity=23.3560090702948, Blast_Score=80, Evalue=7e-15,
Organism=Escherichia coli, GI1789201, Length=221, Percent_Identity=24.8868778280543, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193202679, Length=608, Percent_Identity=24.5065789473684, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17531443, Length=585, Percent_Identity=23.5897435897436, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324667, Length=213, Percent_Identity=29.5774647887324, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24654656, Length=462, Percent_Identity=26.1904761904762, Blast_Score=153, Evalue=4e-37,
Organism=Drosophila melanogaster, GI18859661, Length=436, Percent_Identity=25, Blast_Score=105, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 67051; Mature: 66920

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNVSTLVELLRARANHEPDKLAYMFLIDGKTEGPKLTYAELDRLARAIGALLQKHNAKG
CCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCCCC
ERVLLLYPQGLDVMAAFLGSLYGGVIAIPAPPPDAGRLKRALPRLRAIVKDANAKFVFTN
CEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEC
QHLLGVLQAAKLDFPEFEEMTWFASEDIDLELADQWQDPEITPNTLAYLQYTSGSTSTPK
HHHHHHHHHHHCCCCCHHHEEEECCCCCCEEECCCCCCCCCCCCEEEEEEEECCCCCCCC
GVMISHHNIMHHCAYLQKACGYDTESVSITWMPYFHDYGLVEGLTVPIYNGHPCYVMSPM
CEEEECHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCEEECHH
SFIKQPVRWLQAISRYRGTHSQAPNFAYEQCIRRVTDAQLATLDLSSWVAAGNAAEPINP
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCCCCCH
RVLEEFFEKFAPSGFKWETFAPAYGLAENTLLVSTSPRNTPPVLCLVEKTKIEQNKIVEA
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCEEEE
TTWDDGVRAIPGCGRLVCETQVAIVNPDTLTRCGTDEVGEVWVADPSVAGGYWQRPQESE
ECCCCCCCCCCCCCCEEEEEEEEEECCCHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCC
STFRAKIAGNNQVSFLRTGDLGFIKGGELFITGRIKDLIIIRGTNHYPQDLEWTVQEIHP
CCEEEEECCCCEEEEEEECCEEEEECCCEEEEECEEEEEEEECCCCCCCCHHHHHHHHCH
ALRPDYGAAFSIDVDGVEQLVVVQEVKRKPEEEFNSDEVLTNIRQAIAEIHELQAYAVVL
HHCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHCCEEEEEE
VKPGNVLKTSSGKIQRRACKASFLAGELEVLADWSENPKYTASYRRLQGEVNSLLEKVQV
ECCCCEEECCCCHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHC
TH
CC
>Mature Secondary Structure 
TNVSTLVELLRARANHEPDKLAYMFLIDGKTEGPKLTYAELDRLARAIGALLQKHNAKG
CCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCCCC
ERVLLLYPQGLDVMAAFLGSLYGGVIAIPAPPPDAGRLKRALPRLRAIVKDANAKFVFTN
CEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEC
QHLLGVLQAAKLDFPEFEEMTWFASEDIDLELADQWQDPEITPNTLAYLQYTSGSTSTPK
HHHHHHHHHHHCCCCCHHHEEEECCCCCCEEECCCCCCCCCCCCEEEEEEEECCCCCCCC
GVMISHHNIMHHCAYLQKACGYDTESVSITWMPYFHDYGLVEGLTVPIYNGHPCYVMSPM
CEEEECHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCEEECHH
SFIKQPVRWLQAISRYRGTHSQAPNFAYEQCIRRVTDAQLATLDLSSWVAAGNAAEPINP
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCCCCCH
RVLEEFFEKFAPSGFKWETFAPAYGLAENTLLVSTSPRNTPPVLCLVEKTKIEQNKIVEA
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCEEEE
TTWDDGVRAIPGCGRLVCETQVAIVNPDTLTRCGTDEVGEVWVADPSVAGGYWQRPQESE
ECCCCCCCCCCCCCCEEEEEEEEEECCCHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCC
STFRAKIAGNNQVSFLRTGDLGFIKGGELFITGRIKDLIIIRGTNHYPQDLEWTVQEIHP
CCEEEEECCCCEEEEEEECCEEEEECCCEEEEECEEEEEEEECCCCCCCCHHHHHHHHCH
ALRPDYGAAFSIDVDGVEQLVVVQEVKRKPEEEFNSDEVLTNIRQAIAEIHELQAYAVVL
HHCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHCCEEEEEE
VKPGNVLKTSSGKIQRRACKASFLAGELEVLADWSENPKYTASYRRLQGEVNSLLEKVQV
ECCCCEEECCCCHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHC
TH
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]