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The map label for this gene is btuB [H]

Identifier: 17231520

GI number: 17231520

Start: 4853111

End: 4853974

Strand: Direct

Name: btuB [H]

Synonym: alr4028

Alternate gene names: 17231520

Gene position: 4853111-4853974 (Clockwise)

Preceding gene: 17231519

Following gene: 17231521

Centisome position: 75.67

GC content: 40.62

Gene sequence:

>864_bases
ATGCGTACTCAGTTTATTGCCAGATGGGGAACTCAACTTATCGTTTCTAGTTTGACTATCACCGCAGGGATGCTGTGGGA
AAATTCCAGCATGATAGCCACAGAAGTTCTACAAATATCCGAGTTTCCGCAAGAGGAAGGTTCAGCCAAAGACTTACAAC
CAGTTACAGAAGTAATATCTCAAGATACGACAGAAACCGAACCCGACATTCAACTGACAGTCATTGACAAACTCCTCAAT
GAACCTGTATTTTCCCCTTTTCGCCGCGAGGGAACAGTAAAAGATTCCACCCGTCCGGTGTATGTGATTACTGGCGAAGA
AATGGAAGCACAGGGTGCAATAACTGTCAGAGAAGCACTGCGATTTCTTCCTGGTATTTTGGGTGACGGTACAGTTGGGA
CAGAAGTAAACGCCTTAAGTGGTCAATTTATTCGCGGTTCTAATACCGGACAAGTATTAATCTTATTGGATGGCAGACCA
ATTAATAATTTAGGTGGTGGTGGTTTCGATTTGTCAGAATTTACCACTAATAATATTGAAAGAGTTGAAGTCTTACCAGG
AGGTGGTTCAACACTTTATGGTTCAGATGCAATAGGCGGGGTAATTAATATTGTTACCCGTCGTCCTACAGAGAAAATCA
CCACAGAAACCAAAGTTAATTTCGGTTCCTATGGACTTAACCAACAAAGCATTCAAAATAGTGGAAAATCAGGTGATATT
TCTTGGGTAATTGGTTACAACCGTACCCAAGCACAAAATAATTATCGCTTTACTATTCCTGAAGCTAACTTTGAGGGAAC
AAGAAGAAATAATGACGCACTCTTTAATAACTTTAACGTCAAACTAGAGGCAAATTTCGGTTAA

Upstream 100 bases:

>100_bases
AAACATAGCAATTTTCCTACTGGCTGAGAGTTTTTTACCGAAAAAGCTCAAATTTCTACTTGAGAAAAATTTTTAGTTAT
ACAGGTGGCTTCAATTACCA

Downstream 100 bases:

>100_bases
CGTAACACCTTAACCCTCTCCACCCTATATTTAGGTAAAGAACAGGGAGTACCGGGAGGTGTACCTATTCCTGTACCCGA
ATTTGGACAGGGATTTTTTA

Product: hypothetical protein

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MRTQFIARWGTQLIVSSLTITAGMLWENSSMIATEVLQISEFPQEEGSAKDLQPVTEVISQDTTETEPDIQLTVIDKLLN
EPVFSPFRREGTVKDSTRPVYVITGEEMEAQGAITVREALRFLPGILGDGTVGTEVNALSGQFIRGSNTGQVLILLDGRP
INNLGGGGFDLSEFTTNNIERVEVLPGGGSTLYGSDAIGGVINIVTRRPTEKITTETKVNFGSYGLNQQSIQNSGKSGDI
SWVIGYNRTQAQNNYRFTIPEANFEGTRRNNDALFNNFNVKLEANFG

Sequences:

>Translated_287_residues
MRTQFIARWGTQLIVSSLTITAGMLWENSSMIATEVLQISEFPQEEGSAKDLQPVTEVISQDTTETEPDIQLTVIDKLLN
EPVFSPFRREGTVKDSTRPVYVITGEEMEAQGAITVREALRFLPGILGDGTVGTEVNALSGQFIRGSNTGQVLILLDGRP
INNLGGGGFDLSEFTTNNIERVEVLPGGGSTLYGSDAIGGVINIVTRRPTEKITTETKVNFGSYGLNQQSIQNSGKSGDI
SWVIGYNRTQAQNNYRFTIPEANFEGTRRNNDALFNNFNVKLEANFG
>Mature_287_residues
MRTQFIARWGTQLIVSSLTITAGMLWENSSMIATEVLQISEFPQEEGSAKDLQPVTEVISQDTTETEPDIQLTVIDKLLN
EPVFSPFRREGTVKDSTRPVYVITGEEMEAQGAITVREALRFLPGILGDGTVGTEVNALSGQFIRGSNTGQVLILLDGRP
INNLGGGGFDLSEFTTNNIERVEVLPGGGSTLYGSDAIGGVINIVTRRPTEKITTETKVNFGSYGLNQQSIQNSGKSGDI
SWVIGYNRTQAQNNYRFTIPEANFEGTRRNNDALFNNFNVKLEANFG

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=142, Percent_Identity=38.0281690140845, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788478, Length=129, Percent_Identity=34.1085271317829, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 31363; Mature: 31363

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTQFIARWGTQLIVSSLTITAGMLWENSSMIATEVLQISEFPQEEGSAKDLQPVTEVIS
CCCCHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
QDTTETEPDIQLTVIDKLLNEPVFSPFRREGTVKDSTRPVYVITGEEMEAQGAITVREAL
CCCCCCCCCEEEEEHHHHHCCCCCCCHHHCCCCCCCCCCEEEEECCCCCCCCCEEHHHHH
RFLPGILGDGTVGTEVNALSGQFIRGSNTGQVLILLDGRPINNLGGGGFDLSEFTTNNIE
HHHCCCCCCCCCCCEEECCCCCEEECCCCCEEEEEECCCCCCCCCCCCCCHHHHCCCCCE
RVEVLPGGGSTLYGSDAIGGVINIVTRRPTEKITTETKVNFGSYGLNQQSIQNSGKSGDI
EEEEECCCCCEEECCCHHCHHEEEEECCCCHHHCCEEEEECCCCCCCHHHHHCCCCCCCE
SWVIGYNRTQAQNNYRFTIPEANFEGTRRNNDALFNNFNVKLEANFG
EEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEEEECCC
>Mature Secondary Structure
MRTQFIARWGTQLIVSSLTITAGMLWENSSMIATEVLQISEFPQEEGSAKDLQPVTEVIS
CCCCHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
QDTTETEPDIQLTVIDKLLNEPVFSPFRREGTVKDSTRPVYVITGEEMEAQGAITVREAL
CCCCCCCCCEEEEEHHHHHCCCCCCCHHHCCCCCCCCCCEEEEECCCCCCCCCEEHHHHH
RFLPGILGDGTVGTEVNALSGQFIRGSNTGQVLILLDGRPINNLGGGGFDLSEFTTNNIE
HHHCCCCCCCCCCCEEECCCCCEEECCCCCEEEEEECCCCCCCCCCCCCCHHHHCCCCCE
RVEVLPGGGSTLYGSDAIGGVINIVTRRPTEKITTETKVNFGSYGLNQQSIQNSGKSGDI
EEEEECCCCCEEECCCHHCHHEEEEECCCCHHHCCEEEEECCCCCCCHHHHHCCCCCCCE
SWVIGYNRTQAQNNYRFTIPEANFEGTRRNNDALFNNFNVKLEANFG
EEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA