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The map label for this gene is nth [H]

Identifier: 17231462

GI number: 17231462

Start: 4786746

End: 4787417

Strand: Reverse

Name: nth [H]

Synonym: all3970

Alternate gene names: 17231462

Gene position: 4787417-4786746 (Counterclockwise)

Preceding gene: 17231463

Following gene: 17231461

Centisome position: 74.64

GC content: 45.98

Gene sequence:

>672_bases
GTGAGTACTACCACCCGCAAATCCCTTTCTAAAAAGCAACGGGCGTTAGAAATTCTCTCTCGCCTGAAGCGTCTTTATCC
CGATGCCACTTGCTCCTTAAACTACACCACAACCGTGCAATTGCTGGTGGCAACAATTCTCTCAGCCCAGTGTACAGATG
AGCGAGTCAATCTAGTTACACCAGCTTTATTTAGCCGCTTTCCTGATGCGCCTAGTCTAGCGAATGCTGATTTAACAGAG
TTAGAAAACCTAGTACGCTCTACAGGGTTTTATCGTAATAAAGCCAAAAATATTCAAGCCGCCTGTCGCATGATTGTCAG
TGAGTTTAACTCTGCGGTTCCCAACACAATGGAACAGTTGTTAAAACTCCCTGGTGTGGCGCGGAAGACCGCAAATGTGG
TTTTAGCCCATGCTTATGGCATCAATGCAGGGGTAACAGTAGATACTCATGTCAAGCGCCTCAGTCAACGCCTCGGTTTA
ACTAAATATCCAGACCCCGTTCACATTGAACAGGATTTAATGAAGTTATTACCGCAACCAGATTGGGAAAATTGGTCAAT
TCGGCTAATTTATCATGGTCGCGCTGTCTGTAAAGCCCGTTCTCCTGTATGCGAGGCGTGCGAATTGGCGGATTTATGCC
CATCTGCCACGAAAACAATTATTGTTGGCTAG

Upstream 100 bases:

>100_bases
AGACTGGTCTGGTGTTACTCTTAGGGCTGGGAATTTTTCTGATTGTGAAAGAAACCACCCAATTAACTACCCAATTGGAG
TGGGTACAAAAATTGTTCCA

Downstream 100 bases:

>100_bases
ACGGACAAAAATAGAGGCGATTCGCCTTTCATAAATGTAGAATGGAAAATGCTGTCTTTAAATTGTTGAATTAATTTATG
GCAAAGAAGAGTATGATTGA

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MSTTTRKSLSKKQRALEILSRLKRLYPDATCSLNYTTTVQLLVATILSAQCTDERVNLVTPALFSRFPDAPSLANADLTE
LENLVRSTGFYRNKAKNIQAACRMIVSEFNSAVPNTMEQLLKLPGVARKTANVVLAHAYGINAGVTVDTHVKRLSQRLGL
TKYPDPVHIEQDLMKLLPQPDWENWSIRLIYHGRAVCKARSPVCEACELADLCPSATKTIIVG

Sequences:

>Translated_223_residues
MSTTTRKSLSKKQRALEILSRLKRLYPDATCSLNYTTTVQLLVATILSAQCTDERVNLVTPALFSRFPDAPSLANADLTE
LENLVRSTGFYRNKAKNIQAACRMIVSEFNSAVPNTMEQLLKLPGVARKTANVVLAHAYGINAGVTVDTHVKRLSQRLGL
TKYPDPVHIEQDLMKLLPQPDWENWSIRLIYHGRAVCKARSPVCEACELADLCPSATKTIIVG
>Mature_222_residues
STTTRKSLSKKQRALEILSRLKRLYPDATCSLNYTTTVQLLVATILSAQCTDERVNLVTPALFSRFPDAPSLANADLTEL
ENLVRSTGFYRNKAKNIQAACRMIVSEFNSAVPNTMEQLLKLPGVARKTANVVLAHAYGINAGVTVDTHVKRLSQRLGLT
KYPDPVHIEQDLMKLLPQPDWENWSIRLIYHGRAVCKARSPVCEACELADLCPSATKTIIVG

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=179, Percent_Identity=31.2849162011173, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1787920, Length=213, Percent_Identity=36.6197183098592, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1789331, Length=159, Percent_Identity=28.9308176100629, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17554540, Length=182, Percent_Identity=34.0659340659341, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324530, Length=198, Percent_Identity=30.8080808080808, Blast_Score=99, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6319304, Length=178, Percent_Identity=30.3370786516854, Blast_Score=82, Evalue=7e-17,
Organism=Drosophila melanogaster, GI45550361, Length=183, Percent_Identity=33.879781420765, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24711; Mature: 24580

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTTRKSLSKKQRALEILSRLKRLYPDATCSLNYTTTVQLLVATILSAQCTDERVNLVT
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHCCCCHHHHHH
PALFSRFPDAPSLANADLTELENLVRSTGFYRNKAKNIQAACRMIVSEFNSAVPNTMEQL
HHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LKLPGVARKTANVVLAHAYGINAGVTVDTHVKRLSQRLGLTKYPDPVHIEQDLMKLLPQP
HHCCCCHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCC
DWENWSIRLIYHGRAVCKARSPVCEACELADLCPSATKTIIVG
CCCCCEEEEEECCCHHHHHCCCHHHHHHHHHHCCCCCCEEECC
>Mature Secondary Structure 
STTTRKSLSKKQRALEILSRLKRLYPDATCSLNYTTTVQLLVATILSAQCTDERVNLVT
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHCCCCHHHHHH
PALFSRFPDAPSLANADLTELENLVRSTGFYRNKAKNIQAACRMIVSEFNSAVPNTMEQL
HHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LKLPGVARKTANVVLAHAYGINAGVTVDTHVKRLSQRLGLTKYPDPVHIEQDLMKLLPQP
HHCCCCHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCC
DWENWSIRLIYHGRAVCKARSPVCEACELADLCPSATKTIIVG
CCCCCEEEEEECCCHHHHHCCCHHHHHHHHHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]