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The map label for this gene is nusA [H]

Identifier: 17231321

GI number: 17231321

Start: 4628177

End: 4629454

Strand: Direct

Name: nusA [H]

Synonym: alr3829

Alternate gene names: 17231321

Gene position: 4628177-4629454 (Clockwise)

Preceding gene: 17231320

Following gene: 17231322

Centisome position: 72.16

GC content: 44.99

Gene sequence:

>1278_bases
ATGTCAATGGTTAGTTTACCAGGATTAAAAGATTTAATAGAAAGTATTAGTCGTGAGCGGAATTTGCCCCGATTAGCGGT
TCAGGCTGCTATTAGAGAAGCATTACTTAAGGGTTATGAACGTTATCGTCGCGCTCAAAACATCGAGCGCAAACAGTTTG
ATGAAGATTATTTTGATAACTTTGAAGTCGAGCTAGATATTGATGAGGAAGGATTTCGCGTTCTTTCCACAAAATCCATT
GTTGAAGAAGTTAATAATTCTGACCATCAAATTTCCTTAGATGAAGTGCAGCAAGTAGCTCCCGAAGCCCAATTGGGGGA
CTCAGTGGTACTAGATGTCACTCCAGACCAAGGTGAATTTGGGCGCATGGCTGCCATGCAAACCAAGCAAGTACTGGCAC
AAAAACTACGAGATCAACAGCGCCAGATGGTGCAAGAAGAGTTCCAAGACTTAGAAGGCACAGTTCTCCAGGCCAGAGTT
TTGCGATTTGAGCGCCAATCAGTAATTCTGGCAGTTAGCAGCACCTTTGGACAACCAGAAGTAGAAGCCGAATTACCCAA
GCGCGAACAATTGCCCAATGACAATTATCGGGCAAATGCCACATTTAAGGTTTATCTCAAAAAAGTTTCTCAAGGTCAGC
AACGAGGGCCACAGTTATTAGTATCCCGTGCTGATGCAGGTTTAGTAGTTTATCTATTTGCCAACGAAGTACCAGAAATT
GAAGACGAAGTGGTACGGATAGTTGCCGTAGCCAGGGAGGCAAACCCCCCTTCCCGCTATGTAGGCCCAAGGACTAAAAT
AGCAGTAGATACCCTTGATCGTGATGTAGATCCCGTAGGAGCTTGTATTGGTGCTAGGGGATCACGCATTCAGGTAGTAG
TCAACGAATTACGCGGCGAAAAAATTGACGTGATTCGCTGGTCTCCAGACCCGGCAACATATATTGCGAATGCCCTCAGC
CCGGCGCGAGTCGATGAAGTGCGCCTCATGGACCCAGAAACTCGACAAACTCACGTATTAGTCGCGGAAGACCAACTGAG
TTTGGCTATTGGCAAAGAAGGGCAAAACGTGCGATTAGCTGCCCGATTGACTGGCTGGAAAATAGATATTAAAGATAAAG
CCAAGTATGACCAAGTAGCCGAAGATGCTAAATTTGTGGCGGCGCGTGCAAAATATCAACTAGAGGAAGATGACATCGAA
TCAGACGAGCTAGACTATGAAGAAAATCAACAAGGAGAATTAGAAGACGAATCTTTTGACCCCAACTATGAAGAGTAA

Upstream 100 bases:

>100_bases
AATTTTCCTCCGACTCCCTTGTTACCCTATCTTTTTATTTTCCCTATGCCCAGTACTCAATACCCAATCCCCAGTCCCGA
ATATATAAAGGAGATTGCTT

Downstream 100 bases:

>100_bases
TTTCTTAGCCTTAAGGATTTCTGGTAAGATAGACATTAGTATTAGTCTTAATTATTACTAAAAATCAGGCAACATAAAAA
TCAATCAGAGTTGGCAAGTC

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MSMVSLPGLKDLIESISRERNLPRLAVQAAIREALLKGYERYRRAQNIERKQFDEDYFDNFEVELDIDEEGFRVLSTKSI
VEEVNNSDHQISLDEVQQVAPEAQLGDSVVLDVTPDQGEFGRMAAMQTKQVLAQKLRDQQRQMVQEEFQDLEGTVLQARV
LRFERQSVILAVSSTFGQPEVEAELPKREQLPNDNYRANATFKVYLKKVSQGQQRGPQLLVSRADAGLVVYLFANEVPEI
EDEVVRIVAVAREANPPSRYVGPRTKIAVDTLDRDVDPVGACIGARGSRIQVVVNELRGEKIDVIRWSPDPATYIANALS
PARVDEVRLMDPETRQTHVLVAEDQLSLAIGKEGQNVRLAARLTGWKIDIKDKAKYDQVAEDAKFVAARAKYQLEEDDIE
SDELDYEENQQGELEDESFDPNYEE

Sequences:

>Translated_425_residues
MSMVSLPGLKDLIESISRERNLPRLAVQAAIREALLKGYERYRRAQNIERKQFDEDYFDNFEVELDIDEEGFRVLSTKSI
VEEVNNSDHQISLDEVQQVAPEAQLGDSVVLDVTPDQGEFGRMAAMQTKQVLAQKLRDQQRQMVQEEFQDLEGTVLQARV
LRFERQSVILAVSSTFGQPEVEAELPKREQLPNDNYRANATFKVYLKKVSQGQQRGPQLLVSRADAGLVVYLFANEVPEI
EDEVVRIVAVAREANPPSRYVGPRTKIAVDTLDRDVDPVGACIGARGSRIQVVVNELRGEKIDVIRWSPDPATYIANALS
PARVDEVRLMDPETRQTHVLVAEDQLSLAIGKEGQNVRLAARLTGWKIDIKDKAKYDQVAEDAKFVAARAKYQLEEDDIE
SDELDYEENQQGELEDESFDPNYEE
>Mature_424_residues
SMVSLPGLKDLIESISRERNLPRLAVQAAIREALLKGYERYRRAQNIERKQFDEDYFDNFEVELDIDEEGFRVLSTKSIV
EEVNNSDHQISLDEVQQVAPEAQLGDSVVLDVTPDQGEFGRMAAMQTKQVLAQKLRDQQRQMVQEEFQDLEGTVLQARVL
RFERQSVILAVSSTFGQPEVEAELPKREQLPNDNYRANATFKVYLKKVSQGQQRGPQLLVSRADAGLVVYLFANEVPEIE
DEVVRIVAVAREANPPSRYVGPRTKIAVDTLDRDVDPVGACIGARGSRIQVVVNELRGEKIDVIRWSPDPATYIANALSP
ARVDEVRLMDPETRQTHVLVAEDQLSLAIGKEGQNVRLAARLTGWKIDIKDKAKYDQVAEDAKFVAARAKYQLEEDDIES
DELDYEENQQGELEDESFDPNYEE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=368, Percent_Identity=34.7826086956522, Blast_Score=188, Evalue=5e-49,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 48149; Mature: 48018

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMVSLPGLKDLIESISRERNLPRLAVQAAIREALLKGYERYRRAQNIERKQFDEDYFDN
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHCC
FEVELDIDEEGFRVLSTKSIVEEVNNSDHQISLDEVQQVAPEAQLGDSVVLDVTPDQGEF
EEEEEEECCCCCEEHHHHHHHHHHCCCCCEEEHHHHHHHCCHHHCCCEEEEEECCCCCCH
GRMAAMQTKQVLAQKLRDQQRQMVQEEFQDLEGTVLQARVLRFERQSVILAVSSTFGQPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCC
VEAELPKREQLPNDNYRANATFKVYLKKVSQGQQRGPQLLVSRADAGLVVYLFANEVPEI
CCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCCH
EDEVVRIVAVAREANPPSRYVGPRTKIAVDTLDRDVDPVGACIGARGSRIQVVVNELRGE
HHHHHHHHHHHHCCCCCHHCCCCCCEEEEEHHCCCCCHHHHHHCCCCCEEEEEHHHHCCC
KIDVIRWSPDPATYIANALSPARVDEVRLMDPETRQTHVLVAEDQLSLAIGKEGQNVRLA
EEEEEEECCCHHHHHHHCCCCCCCCCEEECCCCCCCEEEEEECCCEEEEECCCCCCEEEE
ARLTGWKIDIKDKAKYDQVAEDAKFVAARAKYQLEEDDIESDELDYEENQQGELEDESFD
EEEECEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PNYEE
CCCCC
>Mature Secondary Structure 
SMVSLPGLKDLIESISRERNLPRLAVQAAIREALLKGYERYRRAQNIERKQFDEDYFDN
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHCC
FEVELDIDEEGFRVLSTKSIVEEVNNSDHQISLDEVQQVAPEAQLGDSVVLDVTPDQGEF
EEEEEEECCCCCEEHHHHHHHHHHCCCCCEEEHHHHHHHCCHHHCCCEEEEEECCCCCCH
GRMAAMQTKQVLAQKLRDQQRQMVQEEFQDLEGTVLQARVLRFERQSVILAVSSTFGQPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCC
VEAELPKREQLPNDNYRANATFKVYLKKVSQGQQRGPQLLVSRADAGLVVYLFANEVPEI
CCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCCH
EDEVVRIVAVAREANPPSRYVGPRTKIAVDTLDRDVDPVGACIGARGSRIQVVVNELRGE
HHHHHHHHHHHHCCCCCHHCCCCCCEEEEEHHCCCCCHHHHHHCCCCCEEEEEHHHHCCC
KIDVIRWSPDPATYIANALSPARVDEVRLMDPETRQTHVLVAEDQLSLAIGKEGQNVRLA
EEEEEEECCCHHHHHHHCCCCCCCCCEEECCCCCCCEEEEEECCCEEEEECCCCCCEEEE
ARLTGWKIDIKDKAKYDQVAEDAKFVAARAKYQLEEDDIESDELDYEENQQGELEDESFD
EEEECEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PNYEE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]