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The map label for this gene is pknE [H]

Identifier: 17231224

GI number: 17231224

Start: 4506734

End: 4508437

Strand: Direct

Name: pknE [H]

Synonym: alr3732

Alternate gene names: 17231224

Gene position: 4506734-4508437 (Clockwise)

Preceding gene: 17231223

Following gene: 17231229

Centisome position: 70.27

GC content: 38.67

Gene sequence:

>1704_bases
ATGATCGGTAAAGTACTGCAAGCACGCTACCAAATTGTTCAAAACCTGGGTTCAGGGGTATTTGGACAAACATATATTGC
TGTAGATATCAATTACCCACATCAACCTAAATGTGTTGTTAAACAACTTAAGGTTAACAGTTTCCACTCTAGTCACCTAG
ATACAATCAGATTACGTTTTCTGACAGAAACTGAAACCCTCAAGCACTTGGGACAACACCCCCAGATTCCCAATTTCATT
GCTTGTTTTGAAGAAAATGAGCGTTTCTACTTAGTACAAGAGTATATCGGCGGACACGCTTTAACGGCAGAATTGCCAAT
TGCTCAAAATTGGGGTTCTGTGTGGCGCGAAGATGAAGTTATTACATTTCTAGAAGATGCGTTAAGTATTTTACAGTTTG
TGCATTCGCAAGGTGTGATTCATTGCGATGTTAAACCAGAAAATTTAATCAGACGTGCTGTTAATGGCAAATTAGTTTTA
ATTGACTTTGGTTCTATCCAGTCAGTGAATTTTGGCATAGATGAACAAGTATCTATCTATCAGGTTCCGGCTACTTCCTT
GGGATACATACCACCAGAGCAATTTATTGGTAAAACTCAAATCAACAGTGATATCTATGCTTTAGGCATGATTGCTATCC
AGGCTTTAACTGGGCTAGAACCACTGCAATTAAAAATAGATCCTGATAGTAATGAAATCATTTGGCGTTTTGCTGATACG
CCTGTGAGTGACTATCTAGCGGCGATTCTCAGCCAAATGATTCGCTACAATTTCCAAGAACGCTTTCAGTCTGCGGCTGA
GGTGTTGCGTGTACTCCAACAGATGAAATGGGAAACATCCTTGCCACAACTTTTGCAAACACAACAGATAGAATATAGGC
AGGAACCTAATTCTGAAGCGTCGCCGCTAATCACAGGAATGAAAGTAGGACTAGCGGTTAATACCTTATTAATGGGACTG
GGAACTTATTCATTGCTGAGTAATTCTCCAGCTAACACAGAAACAGAACTTTTATATAAAGCGACACAAGAATATCAAGA
CGGCGACTTACAACAAGCGATCGCTCTAGCTAAACTCATACCTTCTCATAGTAATGTATATCCAGATGCTCAAGCCACTA
TAGACGAATGGCAACAGCAATGGCAAACAGCAGCAAAACAATATTCCTTAGCTGAACAAGCATTACTTGAGAATAGATGG
TCAGATGTTTTTGTTGCTGCAAGCGAAGTTCCTAATATTTCATATTGGCAATCTAAAGTTAAGGATGTAGTACAGAAAGC
CAATGTCAATATCGAAGCCCAAACACAAGATTTATTAGCCAAAGCTTATGATAAAGCTAGAGCTAAAGATTTTTCTTCAG
CATTAGAATATTTGCGTCAAATTCCTCAAGAAAGTTCTGCTGGTGCTTTAGTACAAAAAAAACTAGCTGAATACAACCAG
AAAAAACAAATTAGAGCCGCTTATTTTTTACACCAAGCTCGTAAACAAGCATTAGCTGGTAACTTTGATCATGCGGTCAA
CTACCTCCGCAAAATTCCTCAAGGTACTCCTGTTTATGCCCAAGCTCAAGCTAAACTCAATGAGTATACACAAAAGTTAC
GCCCACAAATCCAAAAGGCTAAGTCTTTGATTGCTCATAATTCAGTTATTCAGGTTGGCAATTTACAATCTGAAATTCAA
TTACCAGAAGTAAATATTCGCTAG

Upstream 100 bases:

>100_bases
TAAAGTCGAGCTTAGAGTAATTTGTCTTTCCGGAAACAGCACGCAATCAAGAGAATACTTATATAAGGACTAACTCAGTA
ACCAGCTCATGAACCACCAC

Downstream 100 bases:

>100_bases
TCAAGTCGCTTTGCTCCAATTCAAAACAGGTCAATTTATAGAGACGCGAACCATCACGCCTCTTGACTAAACTGAATAAA
CTTCTACTTACTCAAAATCC

Product: protein serine-threonine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MIGKVLQARYQIVQNLGSGVFGQTYIAVDINYPHQPKCVVKQLKVNSFHSSHLDTIRLRFLTETETLKHLGQHPQIPNFI
ACFEENERFYLVQEYIGGHALTAELPIAQNWGSVWREDEVITFLEDALSILQFVHSQGVIHCDVKPENLIRRAVNGKLVL
IDFGSIQSVNFGIDEQVSIYQVPATSLGYIPPEQFIGKTQINSDIYALGMIAIQALTGLEPLQLKIDPDSNEIIWRFADT
PVSDYLAAILSQMIRYNFQERFQSAAEVLRVLQQMKWETSLPQLLQTQQIEYRQEPNSEASPLITGMKVGLAVNTLLMGL
GTYSLLSNSPANTETELLYKATQEYQDGDLQQAIALAKLIPSHSNVYPDAQATIDEWQQQWQTAAKQYSLAEQALLENRW
SDVFVAASEVPNISYWQSKVKDVVQKANVNIEAQTQDLLAKAYDKARAKDFSSALEYLRQIPQESSAGALVQKKLAEYNQ
KKQIRAAYFLHQARKQALAGNFDHAVNYLRKIPQGTPVYAQAQAKLNEYTQKLRPQIQKAKSLIAHNSVIQVGNLQSEIQ
LPEVNIR

Sequences:

>Translated_567_residues
MIGKVLQARYQIVQNLGSGVFGQTYIAVDINYPHQPKCVVKQLKVNSFHSSHLDTIRLRFLTETETLKHLGQHPQIPNFI
ACFEENERFYLVQEYIGGHALTAELPIAQNWGSVWREDEVITFLEDALSILQFVHSQGVIHCDVKPENLIRRAVNGKLVL
IDFGSIQSVNFGIDEQVSIYQVPATSLGYIPPEQFIGKTQINSDIYALGMIAIQALTGLEPLQLKIDPDSNEIIWRFADT
PVSDYLAAILSQMIRYNFQERFQSAAEVLRVLQQMKWETSLPQLLQTQQIEYRQEPNSEASPLITGMKVGLAVNTLLMGL
GTYSLLSNSPANTETELLYKATQEYQDGDLQQAIALAKLIPSHSNVYPDAQATIDEWQQQWQTAAKQYSLAEQALLENRW
SDVFVAASEVPNISYWQSKVKDVVQKANVNIEAQTQDLLAKAYDKARAKDFSSALEYLRQIPQESSAGALVQKKLAEYNQ
KKQIRAAYFLHQARKQALAGNFDHAVNYLRKIPQGTPVYAQAQAKLNEYTQKLRPQIQKAKSLIAHNSVIQVGNLQSEIQ
LPEVNIR
>Mature_567_residues
MIGKVLQARYQIVQNLGSGVFGQTYIAVDINYPHQPKCVVKQLKVNSFHSSHLDTIRLRFLTETETLKHLGQHPQIPNFI
ACFEENERFYLVQEYIGGHALTAELPIAQNWGSVWREDEVITFLEDALSILQFVHSQGVIHCDVKPENLIRRAVNGKLVL
IDFGSIQSVNFGIDEQVSIYQVPATSLGYIPPEQFIGKTQINSDIYALGMIAIQALTGLEPLQLKIDPDSNEIIWRFADT
PVSDYLAAILSQMIRYNFQERFQSAAEVLRVLQQMKWETSLPQLLQTQQIEYRQEPNSEASPLITGMKVGLAVNTLLMGL
GTYSLLSNSPANTETELLYKATQEYQDGDLQQAIALAKLIPSHSNVYPDAQATIDEWQQQWQTAAKQYSLAEQALLENRW
SDVFVAASEVPNISYWQSKVKDVVQKANVNIEAQTQDLLAKAYDKARAKDFSSALEYLRQIPQESSAGALVQKKLAEYNQ
KKQIRAAYFLHQARKQALAGNFDHAVNYLRKIPQGTPVYAQAQAKLNEYTQKLRPQIQKAKSLIAHNSVIQVGNLQSEIQ
LPEVNIR

Specific function: Probably required for both normal cellular growth and differentiation. Inactivation of pknA leads to colonies that appear light green and rough in the absence of combined nitrogen [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 protein kinase domain [H]

Homologues:

Organism=Homo sapiens, GI38327572, Length=278, Percent_Identity=26.6187050359712, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI38327570, Length=278, Percent_Identity=26.6187050359712, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI38327568, Length=278, Percent_Identity=26.6187050359712, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI38327566, Length=278, Percent_Identity=26.6187050359712, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI38327564, Length=278, Percent_Identity=26.6187050359712, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI38327562, Length=278, Percent_Identity=26.6187050359712, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI62865639, Length=261, Percent_Identity=27.2030651340996, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI62865641, Length=261, Percent_Identity=27.2030651340996, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI62865643, Length=261, Percent_Identity=27.2030651340996, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI27901803, Length=221, Percent_Identity=28.0542986425339, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI10835242, Length=285, Percent_Identity=24.9122807017544, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI7661974, Length=215, Percent_Identity=29.7674418604651, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI148612818, Length=285, Percent_Identity=24.9122807017544, Blast_Score=73, Evalue=8e-13,
Organism=Homo sapiens, GI116008188, Length=269, Percent_Identity=23.0483271375465, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI116008192, Length=269, Percent_Identity=23.0483271375465, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI4759050, Length=291, Percent_Identity=25.085910652921, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI83627721, Length=288, Percent_Identity=22.5694444444444, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI19923570, Length=225, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI83776600, Length=263, Percent_Identity=25.4752851711027, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI55743136, Length=225, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI32528295, Length=259, Percent_Identity=25.4826254826255, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI41872673, Length=269, Percent_Identity=26.3940520446097, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI32528297, Length=237, Percent_Identity=26.5822784810127, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI225637562, Length=209, Percent_Identity=25.8373205741627, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI41281753, Length=278, Percent_Identity=25.8992805755396, Blast_Score=67, Evalue=6e-11,
Organism=Homo sapiens, GI225637560, Length=274, Percent_Identity=26.2773722627737, Blast_Score=67, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17543412, Length=258, Percent_Identity=24.031007751938, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17505246, Length=231, Percent_Identity=24.6753246753247, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI133900707, Length=294, Percent_Identity=25.1700680272109, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI133900709, Length=294, Percent_Identity=25.1700680272109, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71982783, Length=227, Percent_Identity=26.8722466960352, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71982774, Length=227, Percent_Identity=26.8722466960352, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71982766, Length=227, Percent_Identity=26.8722466960352, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71989900, Length=269, Percent_Identity=24.5353159851301, Blast_Score=69, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI71988264, Length=212, Percent_Identity=25.9433962264151, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71989905, Length=269, Percent_Identity=24.5353159851301, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71989893, Length=269, Percent_Identity=24.5353159851301, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI193203107, Length=269, Percent_Identity=24.5353159851301, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71989911, Length=269, Percent_Identity=24.5353159851301, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI72001122, Length=231, Percent_Identity=23.8095238095238, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319917, Length=237, Percent_Identity=27.4261603375527, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6321126, Length=227, Percent_Identity=30.8370044052863, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324557, Length=224, Percent_Identity=26.7857142857143, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6319502, Length=159, Percent_Identity=32.7044025157233, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6320674, Length=229, Percent_Identity=28.3842794759825, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24653966, Length=250, Percent_Identity=28, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24653974, Length=257, Percent_Identity=28.4046692607004, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24653972, Length=257, Percent_Identity=28.4046692607004, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24653970, Length=257, Percent_Identity=28.7937743190661, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24653968, Length=257, Percent_Identity=28.4046692607004, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI17136516, Length=260, Percent_Identity=27.3076923076923, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24647440, Length=218, Percent_Identity=25.2293577981651, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24647442, Length=218, Percent_Identity=25.2293577981651, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24646075, Length=185, Percent_Identity=29.1891891891892, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24646073, Length=185, Percent_Identity=29.1891891891892, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011009
- InterPro:   IPR000719
- InterPro:   IPR017441
- InterPro:   IPR017442
- InterPro:   IPR008271 [H]

Pfam domain/function: PF00069 Pkinase [H]

EC number: =2.7.11.1 [H]

Molecular weight: Translated: 64015; Mature: 64015

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00107 PROTEIN_KINASE_ATP ; PS50011 PROTEIN_KINASE_DOM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGKVLQARYQIVQNLGSGVFGQTYIAVDINYPHQPKCVVKQLKVNSFHSSHLDTIRLRF
CCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEE
LTETETLKHLGQHPQIPNFIACFEENERFYLVQEYIGGHALTAELPIAQNWGSVWREDEV
EHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEHHHHCCEEEEECCCCHHCHHHHHCCCHH
ITFLEDALSILQFVHSQGVIHCDVKPENLIRRAVNGKLVLIDFGSIQSVNFGIDEQVSIY
HHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHCCCCEEEEECCCCCEECCCCCCCCEEE
QVPATSLGYIPPEQFIGKTQINSDIYALGMIAIQALTGLEPLQLKIDPDSNEIIWRFADT
ECCCCCCCCCCHHHHCCCHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEECCC
PVSDYLAAILSQMIRYNFQERFQSAAEVLRVLQQMKWETSLPQLLQTQQIEYRQEPNSEA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCC
SPLITGMKVGLAVNTLLMGLGTYSLLSNSPANTETELLYKATQEYQDGDLQQAIALAKLI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHC
PSHSNVYPDAQATIDEWQQQWQTAAKQYSLAEQALLENRWSDVFVAASEVPNISYWQSKV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHH
KDVVQKANVNIEAQTQDLLAKAYDKARAKDFSSALEYLRQIPQESSAGALVQKKLAEYNQ
HHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
KKQIRAAYFLHQARKQALAGNFDHAVNYLRKIPQGTPVYAQAQAKLNEYTQKLRPQIQKA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KSLIAHNSVIQVGNLQSEIQLPEVNIR
HHHHHHCCEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MIGKVLQARYQIVQNLGSGVFGQTYIAVDINYPHQPKCVVKQLKVNSFHSSHLDTIRLRF
CCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEE
LTETETLKHLGQHPQIPNFIACFEENERFYLVQEYIGGHALTAELPIAQNWGSVWREDEV
EHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEHHHHCCEEEEECCCCHHCHHHHHCCCHH
ITFLEDALSILQFVHSQGVIHCDVKPENLIRRAVNGKLVLIDFGSIQSVNFGIDEQVSIY
HHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHCCCCEEEEECCCCCEECCCCCCCCEEE
QVPATSLGYIPPEQFIGKTQINSDIYALGMIAIQALTGLEPLQLKIDPDSNEIIWRFADT
ECCCCCCCCCCHHHHCCCHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEECCC
PVSDYLAAILSQMIRYNFQERFQSAAEVLRVLQQMKWETSLPQLLQTQQIEYRQEPNSEA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCC
SPLITGMKVGLAVNTLLMGLGTYSLLSNSPANTETELLYKATQEYQDGDLQQAIALAKLI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHC
PSHSNVYPDAQATIDEWQQQWQTAAKQYSLAEQALLENRWSDVFVAASEVPNISYWQSKV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHH
KDVVQKANVNIEAQTQDLLAKAYDKARAKDFSSALEYLRQIPQESSAGALVQKKLAEYNQ
HHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
KKQIRAAYFLHQARKQALAGNFDHAVNYLRKIPQGTPVYAQAQAKLNEYTQKLRPQIQKA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KSLIAHNSVIQVGNLQSEIQLPEVNIR
HHHHHHCCEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7505448; 11759840 [H]