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The map label for this gene is hsdR [H]

Identifier: 17231110

GI number: 17231110

Start: 4368583

End: 4370913

Strand: Direct

Name: hsdR [H]

Synonym: alr3618

Alternate gene names: 17231110

Gene position: 4368583-4370913 (Clockwise)

Preceding gene: 17231106

Following gene: 17231111

Centisome position: 68.11

GC content: 41.66

Gene sequence:

>2331_bases
ATGTCAGAAGTAATTGACAAGAAATCTTTGAGTGAACGTGATATCTGTACAAAGTTTATTACACCTGCTTTAACTAATCG
GGGATGGAATATCAGCACTCAGATTCGTGAGGAAGTGACGCTGACAAAAGGTAGGGTGATTGTTAGGGGTAAGCTTACTA
GTCGAGGTGAGCAGAAACGCGCTGATTATGTGCTGTATCACAAACCGGGTGTACCACTGGCTGTGATTGAAGCTAAAGAT
AATAATCACAGTGTCAGTGCGGGAATGCAGCAAGCGATCGCAACTGGTGAACTAATTGATGTACCATTCATCTTTAGCTC
GAATGGCGACGCTTTTATGATGTGTGACTGTACAGTCACCGAAGGACAGCGAGAACGGGAAATTCCCCTCAATCAGTTCC
CGACACCGGAAGAACTTTGGCAAAAATACTGCGATTGGAAGGGAATTGACTTACATATTCAGCCGATTGTGGCTCAAGAT
TATTATCCTAGTCCTGATAAAAAACAGCCACGCTATTATCAACAGATTGCCATTAACCGGACAATTGAGGCGATCGCTAA
AGGAGAAAACCGCATTCTGTTAGTGATGGCGACGGGTACGGGTAAGACTTTTACAGCTTTTCAGATTATCTGGCGGTTGT
GGAAGTCAGGGGCGAAAAAGCGTATTCTGTTCTTGGCAGACCGCAACATTTTGGTTGATCAAACTAGAGTTAATGACTTT
AAACCCTTTGGCTCAAAAATGACCAAAATTAAGCAGCGACAGATAGATACATCTTACGAGATTTATCTATGTCTGTATCA
GGCGGTAACGGGAAATGAAGAGGCGAAAAACATTTATCGGCAGTTTTCACCAGGTTTTTTTGACTTAATTATTATAGATG
AGTGCCATCGGGGAAGCGCGTCTGAAGATTCGGCATGGCGAGATATTTTGGCATATTTTAGCAGTGCAACGCAGATTGGC
TTGACTGCTACCCCAAGGGAAACAGAAGAAGTTTCTAATAGTCTTTATTTTGGCGATTCAATTTTTACCTATTCGCTCAA
ACAAGGGATTGAAGATGGCTTTTTAGCTCCCTATAAAGTTATTCGCATTGACTTTGATACAGATTTGAGTGGCTGGAAAC
CGAAGCCAGGACAAAGAGATAAATATGGTAAAGAAATTCCTGACCAAGTATTTAATCAGCGAGATTTTGATAGAACTCTG
GTTTTAGAGAAGCGTACCGAGTTAGTCGCGCGGATTATTTCTGATTATCTCAAATCAAGCGATCGCTTTGCCAAAACTAT
CGTTTTTTGTGAGACGACTGACCACGCAGAACGGATGCGGGTGGCGTTGGTGAATGAGAATGCTGATTTGATGGCGGAGA
ATAGCCGCTACATTATGCGAATTACTGGGGATGATGCTCAGGGTAAAGCAGAATTAGATAATTTCATTGACCCTGAAAGT
AAATATCCTACGATTGTTACTACCTCTGAGTTACTGACGACTGGCGTTGATGCTAAAACTTGCAAGTTGATTGTTTTAGA
CCAGCGCATCCTGTCTATGACTAAATTTAAGCAAATTGTTGGTCGCGGTACGCGCATTGATGAAGACTATGGCAAAATGT
TCTTCACCATTATCGATTTTAAGAAAGCAACACAGTTATTTGCTGACCCTGAGTTTGATGGTGAACCTGTACAAATTTAT
CAGCCCAAACCAGTTGATCCAATTGTTCCACCCGATAATGGAGACGATGAGGTAATCATTGATGGTGAGATTACCCGCAA
GCAAAAGCGGGAGAAGTATGTAATTGCAGACGAAGAGGTGTCTGTTGCTTTTATTCGTGAGCAGTACCACGGTAAGGACG
GTAAGCTGATTACGGAATCGATTAAAGACTATACTCGCAAAACAGTTAGTCAGGAGTACGCCTCATTAGATGCTTTCTTG
AAGAAATGGCACTCTACCGAACAAAAGCAGGCTATTATCCAAGAGCTACAGGAATTGGGTGTACCTTTGGAAGCGTTAGA
AAAAGAGATTGGCAAGGGTTTTGACCCATTAGACTTGATTTGTCATGTTGTCTTTGACCAACCGCCACTAACACGGAAAG
AAAGGGCGAACAATGTCCGCAAGCGTAATTATTTTAGCAAGTATAGTGAACAGGCTCGTAAGGTAATAGATGCGCTTTTG
GATAAATATGCGGATGAAGGGATTGAAGATATTGAAAAATTGGATGTGCTGAAGGTTCATCCTTTTGATAAAGTGGGTAC
ACCAATAGAAATTATTCAGCTTTTTGGCAGTAGAAAAGAATATCTTAATGTACTTCAGCAGCTAAAAACGCAACTTTATG
AGATAGCCTAG

Upstream 100 bases:

>100_bases
GGCTGCCATCTGGGAAAATTATAGTTTTATACTTAACAACAGCAAATGAGAAAGCGTAGAATTATACCAAGCATCCTCTT
AACCGAAAGCCGACCCTTTT

Downstream 100 bases:

>100_bases
TTAATCATCTTTAAATTTGTTTTGTATGGACGATCCAATAAAATACATTGACTCATCTATTAAAGGTTTACAGGATAAAC
TATCTACATCTAATTCGGTG

Product: type I restriction-modification enzyme R subunit

Products: NA

Alternate protein names: R.EcoAI [H]

Number of amino acids: Translated: 776; Mature: 775

Protein sequence:

>776_residues
MSEVIDKKSLSERDICTKFITPALTNRGWNISTQIREEVTLTKGRVIVRGKLTSRGEQKRADYVLYHKPGVPLAVIEAKD
NNHSVSAGMQQAIATGELIDVPFIFSSNGDAFMMCDCTVTEGQREREIPLNQFPTPEELWQKYCDWKGIDLHIQPIVAQD
YYPSPDKKQPRYYQQIAINRTIEAIAKGENRILLVMATGTGKTFTAFQIIWRLWKSGAKKRILFLADRNILVDQTRVNDF
KPFGSKMTKIKQRQIDTSYEIYLCLYQAVTGNEEAKNIYRQFSPGFFDLIIIDECHRGSASEDSAWRDILAYFSSATQIG
LTATPRETEEVSNSLYFGDSIFTYSLKQGIEDGFLAPYKVIRIDFDTDLSGWKPKPGQRDKYGKEIPDQVFNQRDFDRTL
VLEKRTELVARIISDYLKSSDRFAKTIVFCETTDHAERMRVALVNENADLMAENSRYIMRITGDDAQGKAELDNFIDPES
KYPTIVTTSELLTTGVDAKTCKLIVLDQRILSMTKFKQIVGRGTRIDEDYGKMFFTIIDFKKATQLFADPEFDGEPVQIY
QPKPVDPIVPPDNGDDEVIIDGEITRKQKREKYVIADEEVSVAFIREQYHGKDGKLITESIKDYTRKTVSQEYASLDAFL
KKWHSTEQKQAIIQELQELGVPLEALEKEIGKGFDPLDLICHVVFDQPPLTRKERANNVRKRNYFSKYSEQARKVIDALL
DKYADEGIEDIEKLDVLKVHPFDKVGTPIEIIQLFGSRKEYLNVLQQLKTQLYEIA

Sequences:

>Translated_776_residues
MSEVIDKKSLSERDICTKFITPALTNRGWNISTQIREEVTLTKGRVIVRGKLTSRGEQKRADYVLYHKPGVPLAVIEAKD
NNHSVSAGMQQAIATGELIDVPFIFSSNGDAFMMCDCTVTEGQREREIPLNQFPTPEELWQKYCDWKGIDLHIQPIVAQD
YYPSPDKKQPRYYQQIAINRTIEAIAKGENRILLVMATGTGKTFTAFQIIWRLWKSGAKKRILFLADRNILVDQTRVNDF
KPFGSKMTKIKQRQIDTSYEIYLCLYQAVTGNEEAKNIYRQFSPGFFDLIIIDECHRGSASEDSAWRDILAYFSSATQIG
LTATPRETEEVSNSLYFGDSIFTYSLKQGIEDGFLAPYKVIRIDFDTDLSGWKPKPGQRDKYGKEIPDQVFNQRDFDRTL
VLEKRTELVARIISDYLKSSDRFAKTIVFCETTDHAERMRVALVNENADLMAENSRYIMRITGDDAQGKAELDNFIDPES
KYPTIVTTSELLTTGVDAKTCKLIVLDQRILSMTKFKQIVGRGTRIDEDYGKMFFTIIDFKKATQLFADPEFDGEPVQIY
QPKPVDPIVPPDNGDDEVIIDGEITRKQKREKYVIADEEVSVAFIREQYHGKDGKLITESIKDYTRKTVSQEYASLDAFL
KKWHSTEQKQAIIQELQELGVPLEALEKEIGKGFDPLDLICHVVFDQPPLTRKERANNVRKRNYFSKYSEQARKVIDALL
DKYADEGIEDIEKLDVLKVHPFDKVGTPIEIIQLFGSRKEYLNVLQQLKTQLYEIA
>Mature_775_residues
SEVIDKKSLSERDICTKFITPALTNRGWNISTQIREEVTLTKGRVIVRGKLTSRGEQKRADYVLYHKPGVPLAVIEAKDN
NHSVSAGMQQAIATGELIDVPFIFSSNGDAFMMCDCTVTEGQREREIPLNQFPTPEELWQKYCDWKGIDLHIQPIVAQDY
YPSPDKKQPRYYQQIAINRTIEAIAKGENRILLVMATGTGKTFTAFQIIWRLWKSGAKKRILFLADRNILVDQTRVNDFK
PFGSKMTKIKQRQIDTSYEIYLCLYQAVTGNEEAKNIYRQFSPGFFDLIIIDECHRGSASEDSAWRDILAYFSSATQIGL
TATPRETEEVSNSLYFGDSIFTYSLKQGIEDGFLAPYKVIRIDFDTDLSGWKPKPGQRDKYGKEIPDQVFNQRDFDRTLV
LEKRTELVARIISDYLKSSDRFAKTIVFCETTDHAERMRVALVNENADLMAENSRYIMRITGDDAQGKAELDNFIDPESK
YPTIVTTSELLTTGVDAKTCKLIVLDQRILSMTKFKQIVGRGTRIDEDYGKMFFTIIDFKKATQLFADPEFDGEPVQIYQ
PKPVDPIVPPDNGDDEVIIDGEITRKQKREKYVIADEEVSVAFIREQYHGKDGKLITESIKDYTRKTVSQEYASLDAFLK
KWHSTEQKQAIIQELQELGVPLEALEKEIGKGFDPLDLICHVVFDQPPLTRKERANNVRKRNYFSKYSEQARKVIDALLD
KYADEGIEDIEKLDVLKVHPFDKVGTPIEIIQLFGSRKEYLNVLQQLKTQLYEIA

Specific function: The EcoAI enzyme recognizes 5'-GAGN(7)GTCA-3'. Subunit R is required for both nuclease and ATPase activities, but not for modification [H]

COG id: COG4096

COG function: function code V; Type I site-specific restriction-modification system, R (restriction) subunit and related helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI226510991, Length=578, Percent_Identity=28.2006920415225, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1788511, Length=423, Percent_Identity=25.531914893617, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6320539, Length=390, Percent_Identity=23.8461538461538, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR006935
- InterPro:   IPR013670
- InterPro:   IPR007409 [H]

Pfam domain/function: PF08463 EcoEI_R_C; PF04313 HSDR_N; PF04851 ResIII [H]

EC number: =3.1.21.3 [H]

Molecular weight: Translated: 88981; Mature: 88850

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVIDKKSLSERDICTKFITPALTNRGWNISTQIREEVTLTKGRVIVRGKLTSRGEQKR
CCCHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHEEECCEEEEEECCCCCCCCCC
ADYVLYHKPGVPLAVIEAKDNNHSVSAGMQQAIATGELIDVPFIFSSNGDAFMMCDCTVT
CCEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCEEEEEEEEEC
EGQREREIPLNQFPTPEELWQKYCDWKGIDLHIQPIVAQDYYPSPDKKQPRYYQQIAINR
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHH
TIEAIAKGENRILLVMATGTGKTFTAFQIIWRLWKSGAKKRILFLADRNILVDQTRVNDF
HHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCCCCC
KPFGSKMTKIKQRQIDTSYEIYLCLYQAVTGNEEAKNIYRQFSPGFFDLIIIDECHRGSA
CCCHHHHHHHHHHHCCCCCEEEEEEEHHHCCCHHHHHHHHHCCCCEEEEEEEECCCCCCC
SEDSAWRDILAYFSSATQIGLTATPRETEEVSNSLYFGDSIFTYSLKQGIEDGFLAPYKV
CCHHHHHHHHHHHHCCCEECEECCCCHHHHHHCCEEECCHHEEEHHHHCCCCCCCCCEEE
IRIDFDTDLSGWKPKPGQRDKYGKEIPDQVFNQRDFDRTLVLEKRTELVARIISDYLKSS
EEEEECCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
DRFAKTIVFCETTDHAERMRVALVNENADLMAENSRYIMRITGDDAQGKAELDNFIDPES
CCCEEEEEEEECCCCHHHEEEEEECCCCCEEECCCEEEEEEECCCCCCCHHHHHCCCCCC
KYPTIVTTSELLTTGVDAKTCKLIVLDQRILSMTKFKQIVGRGTRIDEDYGKMFFTIIDF
CCCEEEEEHHHHHCCCCCHHEEEEEECHHHHHHHHHHHHHCCCCCCCHHHHHEEEHEEEH
KKATQLFADPEFDGEPVQIYQPKPVDPIVPPDNGDDEVIIDGEITRKQKREKYVIADEEV
HHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEECCCHHHHHHCCCEEEECCCC
SVAFIREQYHGKDGKLITESIKDYTRKTVSQEYASLDAFLKKWHSTEQKQAIIQELQELG
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
VPLEALEKEIGKGFDPLDLICHVVFDQPPLTRKERANNVRKRNYFSKYSEQARKVIDALL
CCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKYADEGIEDIEKLDVLKVHPFDKVGTPIEIIQLFGSRKEYLNVLQQLKTQLYEIA
HHHHHCCHHHHHHCCEEEECCCHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEVIDKKSLSERDICTKFITPALTNRGWNISTQIREEVTLTKGRVIVRGKLTSRGEQKR
CCHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHEEECCEEEEEECCCCCCCCCC
ADYVLYHKPGVPLAVIEAKDNNHSVSAGMQQAIATGELIDVPFIFSSNGDAFMMCDCTVT
CCEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCEEEEEEEEEC
EGQREREIPLNQFPTPEELWQKYCDWKGIDLHIQPIVAQDYYPSPDKKQPRYYQQIAINR
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHH
TIEAIAKGENRILLVMATGTGKTFTAFQIIWRLWKSGAKKRILFLADRNILVDQTRVNDF
HHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCCCCC
KPFGSKMTKIKQRQIDTSYEIYLCLYQAVTGNEEAKNIYRQFSPGFFDLIIIDECHRGSA
CCCHHHHHHHHHHHCCCCCEEEEEEEHHHCCCHHHHHHHHHCCCCEEEEEEEECCCCCCC
SEDSAWRDILAYFSSATQIGLTATPRETEEVSNSLYFGDSIFTYSLKQGIEDGFLAPYKV
CCHHHHHHHHHHHHCCCEECEECCCCHHHHHHCCEEECCHHEEEHHHHCCCCCCCCCEEE
IRIDFDTDLSGWKPKPGQRDKYGKEIPDQVFNQRDFDRTLVLEKRTELVARIISDYLKSS
EEEEECCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
DRFAKTIVFCETTDHAERMRVALVNENADLMAENSRYIMRITGDDAQGKAELDNFIDPES
CCCEEEEEEEECCCCHHHEEEEEECCCCCEEECCCEEEEEEECCCCCCCHHHHHCCCCCC
KYPTIVTTSELLTTGVDAKTCKLIVLDQRILSMTKFKQIVGRGTRIDEDYGKMFFTIIDF
CCCEEEEEHHHHHCCCCCHHEEEEEECHHHHHHHHHHHHHCCCCCCCHHHHHEEEHEEEH
KKATQLFADPEFDGEPVQIYQPKPVDPIVPPDNGDDEVIIDGEITRKQKREKYVIADEEV
HHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEECCCHHHHHHCCCEEEECCCC
SVAFIREQYHGKDGKLITESIKDYTRKTVSQEYASLDAFLKKWHSTEQKQAIIQELQELG
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
VPLEALEKEIGKGFDPLDLICHVVFDQPPLTRKERANNVRKRNYFSKYSEQARKVIDALL
CCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKYADEGIEDIEKLDVLKVHPFDKVGTPIEIIQLFGSRKEYLNVLQQLKTQLYEIA
HHHHHCCHHHHHHCCEEEECCCHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]