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The map label for this gene is dnaE-N [H]

Identifier: 17231070

GI number: 17231070

Start: 4321425

End: 4324058

Strand: Reverse

Name: dnaE-N [H]

Synonym: all3578

Alternate gene names: 17231070

Gene position: 4324058-4321425 (Counterclockwise)

Preceding gene: 17231071

Following gene: 17231067

Centisome position: 67.42

GC content: 38.95

Gene sequence:

>2634_bases
ATGTCCTTTGTACCTTTACATATTCATAGTGATTACAGCCTGCTGGATGGGGCTAGTCAGCTACCTGAGTTGATTGATCA
AGCGATCGCCTTGGGTATGAAAGCGATCGCTGTTACCGATCACGGTGTCATGTATGGTGCTATTGAACTACTGAAAATTT
GCCGCAGTAAAAATATTAAGCCCATCATTGGCAACGAAATGTATATCCTGAACGGCGACCTGGAAAAACAAGAACGTGGT
CGCCCTAAATATCATCAAGTTGTTTTAGCTAAAAATACTAAAGGTTACAAAAATTTAGTCAAATTAACGACAATCTCTCA
CCTCCAAGGTGTGCAAGGTAAAGGTATTTTTTCTCGCCCTTGTATTAATAAAGATTTGCTCAAAGAGTATCATGAAGGCT
TAATTGTTACCAGTGCTTGTTTAGGTGGAGAAATTCCCCAAGCAATTCTTAGCAACAGACCAGATGCAGCGCGGAAAGTT
GCCAAGTGGTATAAAGATGTTTTTGGTGATGATTTTTATTTAGAAATTCAAGACCACGGTTCACAAGAAGACCGTATTGT
CAACGTAGAAATTATTAAAATTGCCCGTGAGTTAGAGATTAAATTTGTTGCTACCAATGATTCCCATTACATTTCTTGCT
TTGATGTAGAAGCACACGACGCATTGCTATGTATTCAAACTGGCAAGCTAATTAGTGAAGATAAGCGAATGCGGTATAGC
GGTACAGAATATCTCAAATCTGGTGAAGAGATGAAGTTGCTATTCCGCGACCACTTAACCGATGATGTAATTGCAGAGGC
GATCGCCACCACTGAAGAAGTTGCGGATAAAGTCGAGCCTTACCATATTATGGGTGAGCCTCGCATTCCCAACTACCCAA
TTCCTTCAGGTCATACTCCTGATACTTATGTTGAAGAAGTTGCATGGCAAGGACTATTGGAGAAACTCAATCGTAAATCC
CGCAACGAAGTCGAGCCAGTCTACAGAGAAAGGCTAGAATATGAACTCAAAATGTTGCAACAAATGGGATTCTCCAGCTA
CTTTTTAGTAGTTTGGGACTACATCAAATTTGCTAGAGATAATAATATCCCTGTGGGGCCTGGTCGGGGTTCGGCGGCTG
GTTCCTTAGTAGCATACACAATGGGAATTACTAACATTGATCCGGTACATCATGGGTTACTCTTCGAGCGATTTTTGAAC
CCAGAACGTAAATCCATGCCTGATATCGATACAGATTTCTGTATTGAACAACGGGATAAAGTAATTGAATATGTAACAGA
TAAATATGGAAAAGAAAGAGTCGCCCAAATCATTACTTTTAACCGTCTAACTTCCAAAGCAGTGTTAAAAGATGTCGCCA
GAGTGTTAAACATTCCCTATGGGGAATCCGACAAAATGGCAAAATTAATTCCTGTAGTCCGGGGTAAACCCACCAAACTG
AAGGTGATGATTTCCGATGCTACCCCGGAACCAGAGTTTAAAGAAAAATATGATAACGACCCCAGAGTTCGCCATTGGTT
GGATATGGCGATGCGAATCGAAGGGACTAACAAAACCTTTGGTGTTCACGCTGCCGGTGTAGTTATTTCTGCTGATCCAT
TGGATGAAATCGTCCCACTGCAAAAAAATAATGATGGGTCTGTCATTACTCAATATTTCATGGAAGATCTGGAATCAATG
GGTTTGTTAAAAATGGACTTCTTAGGCTTAAAAAACCTGACGATGATCCAAAAAACCATTGACTTGATTGAAGAAAAACA
TGGTTTTAGAATTGACCCTTACGAAATCACCAGTCAAGAACGTAAAGCACAAAAAATCTTAGCTAAAGGTGAATATAAAA
CTCTGCCAAAAGATGTCCAAAAGACCTATGAATTGTTGGAATCTGGTGAATTAGAAGGCATATTTCAATTAGAATCTTCA
GGGATGAAACAGATAGTAAGAGATTTGAAGCCTTCTAATATTGAAGATATTTCTTCTATCTTGGCACTTTACAGACCAGG
CCCTCTAGATGCTGGATTAATTCCTAAGTTTATTAACCGTAAACATGGGCGGGAAAATATCGATTATGAAAGCCAGCTTT
TAGAACCAATATTAAATGAAACCTATGGAATCATGGTCTATCAGGAGCAAATCATGAAAATTGCTCAAGATATGGCTGGT
TATACCTTAGGACAAGCCGACTTGCTGCGCCGTGCGATGGGTAAAAAGAAAGTTTCGGAGATGCAGAAGCAGCAAGAAAA
ATTTATTGATGGTTCAACTAAAAATGGTGTGAAAAAGCAAGTAGCTGAGAAGCTATTTGATGATATGTTGAAATTTGCCG
AATATTGTTTAAGTTATGATACAGAAGTTTTAACAGTTGAATATGGATTTGTCCCCATTGGCGAGATTGTAGAGAAAGGT
ATTGAGTGTAGTGTATTTAGCATTAATAATAATGGTATTGTTTACACTCAACCCATTGCTCAATGGCATCATCGCGGTAA
ACAAGAAGTATTTGAATATTGCTTAGAAGATGGGTCAATAATTAAGGCAACAAAAGACCACAAATTTATGACTCAAGATG
GCAAGATGTTGCCAATTGATGAAATATTTGAACAAGAGTTGGACTTGTTGCAAGTGAAGGGTTTACCAGAGTAA

Upstream 100 bases:

>100_bases
AAATTCCCTAAAAGCGTTAATCTAAGATTCCGTTGCTAGATAAAATCTGGCTCACAAGAAATCCCAGGGGAAGATTGTGG
GCTAGCGCTGGGAGAGAGTT

Downstream 100 bases:

>100_bases
TTCATCTATGCGTCAGCCTGTGAATTTTGACAGACTGTGGGTGTTGAGATCCCCGACTTTTTAAAAAGTCGGGGATCTAC
GACCTATCATAATGCGCTGC

Product: DNA polymerase III subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 877; Mature: 876

Protein sequence:

>877_residues
MSFVPLHIHSDYSLLDGASQLPELIDQAIALGMKAIAVTDHGVMYGAIELLKICRSKNIKPIIGNEMYILNGDLEKQERG
RPKYHQVVLAKNTKGYKNLVKLTTISHLQGVQGKGIFSRPCINKDLLKEYHEGLIVTSACLGGEIPQAILSNRPDAARKV
AKWYKDVFGDDFYLEIQDHGSQEDRIVNVEIIKIARELEIKFVATNDSHYISCFDVEAHDALLCIQTGKLISEDKRMRYS
GTEYLKSGEEMKLLFRDHLTDDVIAEAIATTEEVADKVEPYHIMGEPRIPNYPIPSGHTPDTYVEEVAWQGLLEKLNRKS
RNEVEPVYRERLEYELKMLQQMGFSSYFLVVWDYIKFARDNNIPVGPGRGSAAGSLVAYTMGITNIDPVHHGLLFERFLN
PERKSMPDIDTDFCIEQRDKVIEYVTDKYGKERVAQIITFNRLTSKAVLKDVARVLNIPYGESDKMAKLIPVVRGKPTKL
KVMISDATPEPEFKEKYDNDPRVRHWLDMAMRIEGTNKTFGVHAAGVVISADPLDEIVPLQKNNDGSVITQYFMEDLESM
GLLKMDFLGLKNLTMIQKTIDLIEEKHGFRIDPYEITSQERKAQKILAKGEYKTLPKDVQKTYELLESGELEGIFQLESS
GMKQIVRDLKPSNIEDISSILALYRPGPLDAGLIPKFINRKHGRENIDYESQLLEPILNETYGIMVYQEQIMKIAQDMAG
YTLGQADLLRRAMGKKKVSEMQKQQEKFIDGSTKNGVKKQVAEKLFDDMLKFAEYCLSYDTEVLTVEYGFVPIGEIVEKG
IECSVFSINNNGIVYTQPIAQWHHRGKQEVFEYCLEDGSIIKATKDHKFMTQDGKMLPIDEIFEQELDLLQVKGLPE

Sequences:

>Translated_877_residues
MSFVPLHIHSDYSLLDGASQLPELIDQAIALGMKAIAVTDHGVMYGAIELLKICRSKNIKPIIGNEMYILNGDLEKQERG
RPKYHQVVLAKNTKGYKNLVKLTTISHLQGVQGKGIFSRPCINKDLLKEYHEGLIVTSACLGGEIPQAILSNRPDAARKV
AKWYKDVFGDDFYLEIQDHGSQEDRIVNVEIIKIARELEIKFVATNDSHYISCFDVEAHDALLCIQTGKLISEDKRMRYS
GTEYLKSGEEMKLLFRDHLTDDVIAEAIATTEEVADKVEPYHIMGEPRIPNYPIPSGHTPDTYVEEVAWQGLLEKLNRKS
RNEVEPVYRERLEYELKMLQQMGFSSYFLVVWDYIKFARDNNIPVGPGRGSAAGSLVAYTMGITNIDPVHHGLLFERFLN
PERKSMPDIDTDFCIEQRDKVIEYVTDKYGKERVAQIITFNRLTSKAVLKDVARVLNIPYGESDKMAKLIPVVRGKPTKL
KVMISDATPEPEFKEKYDNDPRVRHWLDMAMRIEGTNKTFGVHAAGVVISADPLDEIVPLQKNNDGSVITQYFMEDLESM
GLLKMDFLGLKNLTMIQKTIDLIEEKHGFRIDPYEITSQERKAQKILAKGEYKTLPKDVQKTYELLESGELEGIFQLESS
GMKQIVRDLKPSNIEDISSILALYRPGPLDAGLIPKFINRKHGRENIDYESQLLEPILNETYGIMVYQEQIMKIAQDMAG
YTLGQADLLRRAMGKKKVSEMQKQQEKFIDGSTKNGVKKQVAEKLFDDMLKFAEYCLSYDTEVLTVEYGFVPIGEIVEKG
IECSVFSINNNGIVYTQPIAQWHHRGKQEVFEYCLEDGSIIKATKDHKFMTQDGKMLPIDEIFEQELDLLQVKGLPE
>Mature_876_residues
SFVPLHIHSDYSLLDGASQLPELIDQAIALGMKAIAVTDHGVMYGAIELLKICRSKNIKPIIGNEMYILNGDLEKQERGR
PKYHQVVLAKNTKGYKNLVKLTTISHLQGVQGKGIFSRPCINKDLLKEYHEGLIVTSACLGGEIPQAILSNRPDAARKVA
KWYKDVFGDDFYLEIQDHGSQEDRIVNVEIIKIARELEIKFVATNDSHYISCFDVEAHDALLCIQTGKLISEDKRMRYSG
TEYLKSGEEMKLLFRDHLTDDVIAEAIATTEEVADKVEPYHIMGEPRIPNYPIPSGHTPDTYVEEVAWQGLLEKLNRKSR
NEVEPVYRERLEYELKMLQQMGFSSYFLVVWDYIKFARDNNIPVGPGRGSAAGSLVAYTMGITNIDPVHHGLLFERFLNP
ERKSMPDIDTDFCIEQRDKVIEYVTDKYGKERVAQIITFNRLTSKAVLKDVARVLNIPYGESDKMAKLIPVVRGKPTKLK
VMISDATPEPEFKEKYDNDPRVRHWLDMAMRIEGTNKTFGVHAAGVVISADPLDEIVPLQKNNDGSVITQYFMEDLESMG
LLKMDFLGLKNLTMIQKTIDLIEEKHGFRIDPYEITSQERKAQKILAKGEYKTLPKDVQKTYELLESGELEGIFQLESSG
MKQIVRDLKPSNIEDISSILALYRPGPLDAGLIPKFINRKHGRENIDYESQLLEPILNETYGIMVYQEQIMKIAQDMAGY
TLGQADLLRRAMGKKKVSEMQKQQEKFIDGSTKNGVKKQVAEKLFDDMLKFAEYCLSYDTEVLTVEYGFVPIGEIVEKGI
ECSVFSINNNGIVYTQPIAQWHHRGKQEVFEYCLEDGSIIKATKDHKFMTQDGKMLPIDEIFEQELDLLQVKGLPE

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase [H]

COG id: COG0587

COG function: function code L; DNA polymerase III, alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-C family. DnaE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786381, Length=788, Percent_Identity=40.1015228426396, Blast_Score=569, Evalue=1e-163,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011708
- InterPro:   IPR003587
- InterPro:   IPR006141
- InterPro:   IPR004365
- InterPro:   IPR004013
- InterPro:   IPR003141
- InterPro:   IPR016195
- InterPro:   IPR004805 [H]

Pfam domain/function: PF07733 DNA_pol3_alpha; PF02811 PHP; PF01336 tRNA_anti [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 99782; Mature: 99651

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS50817 INTEIN_N_TER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFVPLHIHSDYSLLDGASQLPELIDQAIALGMKAIAVTDHGVMYGAIELLKICRSKNIK
CCEEEEEECCCCHHHCCHHHHHHHHHHHHHHCCHHEEEECCCHHHHHHHHHHHHHCCCCC
PIIGNEMYILNGDLEKQERGRPKYHQVVLAKNTKGYKNLVKLTTISHLQGVQGKGIFSRP
EEECCEEEEECCCCCHHHCCCCCHHEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCC
CINKDLLKEYHEGLIVTSACLGGEIPQAILSNRPDAARKVAKWYKDVFGDDFYLEIQDHG
CCCHHHHHHHHCCEEEEHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCC
SQEDRIVNVEIIKIARELEIKFVATNDSHYISCFDVEAHDALLCIQTGKLISEDKRMRYS
CCCCEEEEHHHHEEHHHCEEEEEEECCCCEEEEEEECCCCEEEEEECCCHHHHCCHHCCC
GTEYLKSGEEMKLLFRDHLTDDVIAEAIATTEEVADKVEPYHIMGEPRIPNYPIPSGHTP
CHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCC
DTYVEEVAWQGLLEKLNRKSRNEVEPVYRERLEYELKMLQQMGFSSYFLVVWDYIKFARD
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
NNIPVGPGRGSAAGSLVAYTMGITNIDPVHHGLLFERFLNPERKSMPDIDTDFCIEQRDK
CCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHH
VIEYVTDKYGKERVAQIITFNRLTSKAVLKDVARVLNIPYGESDKMAKLIPVVRGKPTKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEE
KVMISDATPEPEFKEKYDNDPRVRHWLDMAMRIEGTNKTFGVHAAGVVISADPLDEIVPL
EEEEECCCCCCCHHHHCCCCHHHHHHHHHHHEECCCCCEEEEEECCEEEECCCHHHHCCC
QKNNDGSVITQYFMEDLESMGLLKMDFLGLKNLTMIQKTIDLIEEKHGFRIDPYEITSQE
CCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHCHHH
RKAQKILAKGEYKTLPKDVQKTYELLESGELEGIFQLESSGMKQIVRDLKPSNIEDISSI
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCCHHHHHHH
LALYRPGPLDAGLIPKFINRKHGRENIDYESQLLEPILNETYGIMVYQEQIMKIAQDMAG
HHHHCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHEEEEHHHHHHHHHHHHHC
YTLGQADLLRRAMGKKKVSEMQKQQEKFIDGSTKNGVKKQVAEKLFDDMLKFAEYCLSYD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
TEVLTVEYGFVPIGEIVEKGIECSVFSINNNGIVYTQPIAQWHHRGKQEVFEYCLEDGSI
CEEEEEEECCEEHHHHHHCCCCEEEEEECCCCEEEECCHHHHHHCCHHHHHHHHHCCCCE
IKATKDHKFMTQDGKMLPIDEIFEQELDLLQVKGLPE
EEECCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SFVPLHIHSDYSLLDGASQLPELIDQAIALGMKAIAVTDHGVMYGAIELLKICRSKNIK
CEEEEEECCCCHHHCCHHHHHHHHHHHHHHCCHHEEEECCCHHHHHHHHHHHHHCCCCC
PIIGNEMYILNGDLEKQERGRPKYHQVVLAKNTKGYKNLVKLTTISHLQGVQGKGIFSRP
EEECCEEEEECCCCCHHHCCCCCHHEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCC
CINKDLLKEYHEGLIVTSACLGGEIPQAILSNRPDAARKVAKWYKDVFGDDFYLEIQDHG
CCCHHHHHHHHCCEEEEHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCC
SQEDRIVNVEIIKIARELEIKFVATNDSHYISCFDVEAHDALLCIQTGKLISEDKRMRYS
CCCCEEEEHHHHEEHHHCEEEEEEECCCCEEEEEEECCCCEEEEEECCCHHHHCCHHCCC
GTEYLKSGEEMKLLFRDHLTDDVIAEAIATTEEVADKVEPYHIMGEPRIPNYPIPSGHTP
CHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCC
DTYVEEVAWQGLLEKLNRKSRNEVEPVYRERLEYELKMLQQMGFSSYFLVVWDYIKFARD
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
NNIPVGPGRGSAAGSLVAYTMGITNIDPVHHGLLFERFLNPERKSMPDIDTDFCIEQRDK
CCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHH
VIEYVTDKYGKERVAQIITFNRLTSKAVLKDVARVLNIPYGESDKMAKLIPVVRGKPTKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEE
KVMISDATPEPEFKEKYDNDPRVRHWLDMAMRIEGTNKTFGVHAAGVVISADPLDEIVPL
EEEEECCCCCCCHHHHCCCCHHHHHHHHHHHEECCCCCEEEEEECCEEEECCCHHHHCCC
QKNNDGSVITQYFMEDLESMGLLKMDFLGLKNLTMIQKTIDLIEEKHGFRIDPYEITSQE
CCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHCHHH
RKAQKILAKGEYKTLPKDVQKTYELLESGELEGIFQLESSGMKQIVRDLKPSNIEDISSI
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCCHHHHHHH
LALYRPGPLDAGLIPKFINRKHGRENIDYESQLLEPILNETYGIMVYQEQIMKIAQDMAG
HHHHCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHEEEEHHHHHHHHHHHHHC
YTLGQADLLRRAMGKKKVSEMQKQQEKFIDGSTKNGVKKQVAEKLFDDMLKFAEYCLSYD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
TEVLTVEYGFVPIGEIVEKGIECSVFSINNNGIVYTQPIAQWHHRGKQEVFEYCLEDGSI
CEEEEEEECCEEHHHHHHCCCCEEEEEECCCCEEEECCHHHHHHCCHHHHHHHHHCCCCE
IKATKDHKFMTQDGKMLPIDEIFEQELDLLQVKGLPE
EEECCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231; 9689062; 10734038; 11170467 [H]