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The map label for this gene is ycjG [H]

Identifier: 17231024

GI number: 17231024

Start: 4255207

End: 4256259

Strand: Reverse

Name: ycjG [H]

Synonym: all3532

Alternate gene names: 17231024

Gene position: 4256259-4255207 (Counterclockwise)

Preceding gene: 17231026

Following gene: 17231023

Centisome position: 66.36

GC content: 43.87

Gene sequence:

>1053_bases
ATGCAGGTAGAAGTCATTTTGTTCACGGTCAATAAAAGATTTCCCTTGACTATTAGTCGTGGCACTACAGCAAAGACTAC
AAATGTCTGGGTGAAAATTCTACATGATGACATCGAAGGCTGGGGTGAGGCTTCACCGTTTGGTGTGGGTAATTATGGAC
AATCAACTAATGTCATCAATGACTACTTGCAGCAAATTGTGCCATTCTTAGAACCATTTAGCCCGTTGCAGCGACAGGAA
ATAGAGGAAGTATTTATTAAATATCAGGTTCCTTCCGCAGTGAGGGCGGCGCTGGATATGGCGATGCACGACTGGCTGGG
TAAGTTTGTGGGTTTACCTTTATGGCAAATCTGGGGACTAGATCGCCAGGCTATAGTACCAACTTCTGTTACCATTGGTA
TTGATACACCAGAAGCCGCCAGAATACGGACAAGAGACTGGTTAGAATTTACGGATGTTCGCCTGTTGAAAGTGAAGTTA
GGCAGTCCTGATGGTATTGAAGCAGACAAGAAGATGCTCTTAGCTGTGCAGCAAGAAGCACCAGCACAAGAATTTTTTGT
GGATGCCAACGGGGGTTGGTATTTGGACGATGCGGTAGAAATGTGCAGTTGGCTGGCTGATTTAGGTATTAAGTATATTG
AACAGCCGCTAGTTAGGGGGCGAGAACAAGATTTAATCAAACTGAAAGAAAAATCCCCCCTACCGATATTTGTGGATGAA
AGTTGCTTTAACAGTAAAGATATTCCCCGTTTGGCTACTTATGTGGATGGCATCAATATCAAATTGATGAAATCTGGGGG
TTTAACTGAGGCGCTGCGGATGGTACACACAGCTAAAGCTTGTGGGTTACAAGTGATGTTCGGCTGTTATTCTGACAGTA
CATTATCCAATACGGCTGCGGCGCAATTAGCGCCATTAGCTGATTATTTAGACTTAGACAGTCACCTTAACTTAAGTGAT
GACCCTTTCACTGGTGCATTATTAAAAGAAGGGCGAGTGATACCAAACGATTTACCAGGCTTGGGGGTAAGATATAGTGC
GTCTGCCACTTAA

Upstream 100 bases:

>100_bases
TGTGTTCCCTAATGAATGTAAAAAAGTATAACAGATACCTCAAATAAGCTAAACTTTGTATTTACACTGTTTTTGCTCTC
AGCAAAGATTGAGCGTTGTT

Downstream 100 bases:

>100_bases
TCAACGAGTAGCAATTCTGCTACATGAAGGAACTACTGGAACTGTGGGCAAAACCGGGCTAGCACTTTTACGCTACAGTG
AAGCCTCTATTGTCGCCGTA

Product: muconate cycloisomerase

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MQVEVILFTVNKRFPLTISRGTTAKTTNVWVKILHDDIEGWGEASPFGVGNYGQSTNVINDYLQQIVPFLEPFSPLQRQE
IEEVFIKYQVPSAVRAALDMAMHDWLGKFVGLPLWQIWGLDRQAIVPTSVTIGIDTPEAARIRTRDWLEFTDVRLLKVKL
GSPDGIEADKKMLLAVQQEAPAQEFFVDANGGWYLDDAVEMCSWLADLGIKYIEQPLVRGREQDLIKLKEKSPLPIFVDE
SCFNSKDIPRLATYVDGINIKLMKSGGLTEALRMVHTAKACGLQVMFGCYSDSTLSNTAAAQLAPLADYLDLDSHLNLSD
DPFTGALLKEGRVIPNDLPGLGVRYSASAT

Sequences:

>Translated_350_residues
MQVEVILFTVNKRFPLTISRGTTAKTTNVWVKILHDDIEGWGEASPFGVGNYGQSTNVINDYLQQIVPFLEPFSPLQRQE
IEEVFIKYQVPSAVRAALDMAMHDWLGKFVGLPLWQIWGLDRQAIVPTSVTIGIDTPEAARIRTRDWLEFTDVRLLKVKL
GSPDGIEADKKMLLAVQQEAPAQEFFVDANGGWYLDDAVEMCSWLADLGIKYIEQPLVRGREQDLIKLKEKSPLPIFVDE
SCFNSKDIPRLATYVDGINIKLMKSGGLTEALRMVHTAKACGLQVMFGCYSDSTLSNTAAAQLAPLADYLDLDSHLNLSD
DPFTGALLKEGRVIPNDLPGLGVRYSASAT
>Mature_350_residues
MQVEVILFTVNKRFPLTISRGTTAKTTNVWVKILHDDIEGWGEASPFGVGNYGQSTNVINDYLQQIVPFLEPFSPLQRQE
IEEVFIKYQVPSAVRAALDMAMHDWLGKFVGLPLWQIWGLDRQAIVPTSVTIGIDTPEAARIRTRDWLEFTDVRLLKVKL
GSPDGIEADKKMLLAVQQEAPAQEFFVDANGGWYLDDAVEMCSWLADLGIKYIEQPLVRGREQDLIKLKEKSPLPIFVDE
SCFNSKDIPRLATYVDGINIKLMKSGGLTEALRMVHTAKACGLQVMFGCYSDSTLSNTAAAQLAPLADYLDLDSHLNLSD
DPFTGALLKEGRVIPNDLPGLGVRYSASAT

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, excep

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=300, Percent_Identity=33, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 38879; Mature: 38879

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVEVILFTVNKRFPLTISRGTTAKTTNVWVKILHDDIEGWGEASPFGVGNYGQSTNVIN
CEEEEEEEEECCCCCEEEECCCCCCHHHHEEEEEEHHHCCCCCCCCCCCCCCCCCCHHHH
DYLQQIVPFLEPFSPLQRQEIEEVFIKYQVPSAVRAALDMAMHDWLGKFVGLPLWQIWGL
HHHHHHHHHHCCCCHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCC
DRQAIVPTSVTIGIDTPEAARIRTRDWLEFTDVRLLKVKLGSPDGIEADKKMLLAVQQEA
CCCEECCCEEEEECCCCCCCCCCCCCCHHHCCEEEEEEEECCCCCCCHHHHHEEEECCCC
PAQEFFVDANGGWYLDDAVEMCSWLADLGIKYIEQPLVRGREQDLIKLKEKSPLPIFVDE
CCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEECC
SCFNSKDIPRLATYVDGINIKLMKSGGLTEALRMVHTAKACGLQVMFGCYSDSTLSNTAA
CCCCCCCCCHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHH
AQLAPLADYLDLDSHLNLSDDPFTGALLKEGRVIPNDLPGLGVRYSASAT
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCEECCCCCCCCCEEECCCCC
>Mature Secondary Structure
MQVEVILFTVNKRFPLTISRGTTAKTTNVWVKILHDDIEGWGEASPFGVGNYGQSTNVIN
CEEEEEEEEECCCCCEEEECCCCCCHHHHEEEEEEHHHCCCCCCCCCCCCCCCCCCHHHH
DYLQQIVPFLEPFSPLQRQEIEEVFIKYQVPSAVRAALDMAMHDWLGKFVGLPLWQIWGL
HHHHHHHHHHCCCCHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCC
DRQAIVPTSVTIGIDTPEAARIRTRDWLEFTDVRLLKVKLGSPDGIEADKKMLLAVQQEA
CCCEECCCEEEEECCCCCCCCCCCCCCHHHCCEEEEEEEECCCCCCCHHHHHEEEECCCC
PAQEFFVDANGGWYLDDAVEMCSWLADLGIKYIEQPLVRGREQDLIKLKEKSPLPIFVDE
CCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEECC
SCFNSKDIPRLATYVDGINIKLMKSGGLTEALRMVHTAKACGLQVMFGCYSDSTLSNTAA
CCCCCCCCCHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHH
AQLAPLADYLDLDSHLNLSDDPFTGALLKEGRVIPNDLPGLGVRYSASAT
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCEECCCCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7499381; 9097039; 9278503 [H]