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The map label for this gene is dosC [H]

Identifier: 17230996

GI number: 17230996

Start: 4220669

End: 4221592

Strand: Direct

Name: dosC [H]

Synonym: alr3504

Alternate gene names: 17230996

Gene position: 4220669-4221592 (Clockwise)

Preceding gene: 17230990

Following gene: 17230997

Centisome position: 65.81

GC content: 32.47

Gene sequence:

>924_bases
GTGTCAAAAATAACTAAAAAGGCTGTTAAAAGAGGGAATATGCGTTTAATTCAAATATTAGAGGCTAAACCTAAATCATT
TTCAATTTTATTAAGTATATTTACGGTTGGTCTCATAGGGTTAATTGACTACTGTATTTCTCCTGAAATTTCTGTATCAA
TCTTATATTTACTTCCCATTGGCATTGCTGCATGGTTCGTCAACCAACATACAGGATTTTTCATAGCAGTAAGCAGTGAT
ATTACTAATCTTTTAGTTAATCAAATTCAAGATCAATACCATTTACATCCGTTAATTCATTTTTGGAATGCACTGGTAAT
TTTAAGTTTTTTTCTATTTGTTAGTCATCTGCTTTCACAACTTAAAACTACATTAATCAATTTAGAGAAATTAGCACGTA
CAGATAATCTCACTGGGTTGACCAATCGAGGCTTTTTTTTAGATATAGTCAATAGCGAGATTGAAAAATCTTTGAGGCAC
AGAGAACCTCTCACACTGGCTTATTTTGATGTTGATAATTTCAAACAAATTAACGATCAGTTTGGACATAGTTTTGGCGA
TCGCCTGCTGCAAATAATAGCAAAAAGTACTACTAACACTCTACGTAAAATAGATGTAATTGCTAGACTTGGTGGTGATG
AATTTGCTATTTTATTACCGCGTACAAGTTATGAATCAGCAGAAGTAGTACTTCACCGATTGCATACAGTTCTTCTAGCT
TCTATGAAGAAGGAAAATTTATCGGTAACTTTTAGTATTGGTGCTATTACTTTTAATAATCCGCCAAAATCAGTGGCTGA
AATTATCGAAATTGCTGATAATTTAATGTATTTGGCTAAAAAGAAAGGTAAAAATTTATTGCAGCATGAATTGTGTGACT
GGAAAGTGGGGAGTGAAGAATGGAAGGTTGTGAATAGTAAGTAA

Upstream 100 bases:

>100_bases
ATTGAAAATATCAATGTCTGTCAAGAGTAATATTTCGGTATTTTTATGTTAAGATTTGACAAAATCTAGATAGTGCGTCG
ATAACAACAGCCGTCGTGTT

Downstream 100 bases:

>100_bases
GTTTTGAATTACTATAAATTACGAAAATACCCCGGCTATGTAACCGAGGTGATTAGGAGAAGTCCAAATGTCAATTAAAA
ATATAAATACTGAGTCTAAA

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSKITKKAVKRGNMRLIQILEAKPKSFSILLSIFTVGLIGLIDYCISPEISVSILYLLPIGIAAWFVNQHTGFFIAVSSD
ITNLLVNQIQDQYHLHPLIHFWNALVILSFFLFVSHLLSQLKTTLINLEKLARTDNLTGLTNRGFFLDIVNSEIEKSLRH
REPLTLAYFDVDNFKQINDQFGHSFGDRLLQIIAKSTTNTLRKIDVIARLGGDEFAILLPRTSYESAEVVLHRLHTVLLA
SMKKENLSVTFSIGAITFNNPPKSVAEIIEIADNLMYLAKKKGKNLLQHELCDWKVGSEEWKVVNSK

Sequences:

>Translated_307_residues
MSKITKKAVKRGNMRLIQILEAKPKSFSILLSIFTVGLIGLIDYCISPEISVSILYLLPIGIAAWFVNQHTGFFIAVSSD
ITNLLVNQIQDQYHLHPLIHFWNALVILSFFLFVSHLLSQLKTTLINLEKLARTDNLTGLTNRGFFLDIVNSEIEKSLRH
REPLTLAYFDVDNFKQINDQFGHSFGDRLLQIIAKSTTNTLRKIDVIARLGGDEFAILLPRTSYESAEVVLHRLHTVLLA
SMKKENLSVTFSIGAITFNNPPKSVAEIIEIADNLMYLAKKKGKNLLQHELCDWKVGSEEWKVVNSK
>Mature_306_residues
SKITKKAVKRGNMRLIQILEAKPKSFSILLSIFTVGLIGLIDYCISPEISVSILYLLPIGIAAWFVNQHTGFFIAVSSDI
TNLLVNQIQDQYHLHPLIHFWNALVILSFFLFVSHLLSQLKTTLINLEKLARTDNLTGLTNRGFFLDIVNSEIEKSLRHR
EPLTLAYFDVDNFKQINDQFGHSFGDRLLQIIAKSTTNTLRKIDVIARLGGDEFAILLPRTSYESAEVVLHRLHTVLLAS
MKKENLSVTFSIGAITFNNPPKSVAEIIEIADNLMYLAKKKGKNLLQHELCDWKVGSEEWKVVNSK

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=190, Percent_Identity=35.2631578947368, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1786584, Length=268, Percent_Identity=29.4776119402985, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1787262, Length=180, Percent_Identity=29.4444444444444, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87082007, Length=186, Percent_Identity=30.1075268817204, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787541, Length=161, Percent_Identity=36.0248447204969, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081881, Length=180, Percent_Identity=32.2222222222222, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788381, Length=180, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788956, Length=169, Percent_Identity=35.5029585798817, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788085, Length=162, Percent_Identity=27.7777777777778, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787802, Length=181, Percent_Identity=25.9668508287293, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081974, Length=155, Percent_Identity=26.4516129032258, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=29.8136645962733, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081977, Length=170, Percent_Identity=27.6470588235294, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 34763; Mature: 34632

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKITKKAVKRGNMRLIQILEAKPKSFSILLSIFTVGLIGLIDYCISPEISVSILYLLPI
CCHHHHHHHHCCCHHEEHEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
GIAAWFVNQHTGFFIAVSSDITNLLVNQIQDQYHLHPLIHFWNALVILSFFLFVSHLLSQ
HHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKTTLINLEKLARTDNLTGLTNRGFFLDIVNSEIEKSLRHREPLTLAYFDVDNFKQINDQ
HHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHH
FGHSFGDRLLQIIAKSTTNTLRKIDVIARLGGDEFAILLPRTSYESAEVVLHRLHTVLLA
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
SMKKENLSVTFSIGAITFNNPPKSVAEIIEIADNLMYLAKKKGKNLLQHELCDWKVGSEE
HHCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCC
WKVVNSK
CEEECCC
>Mature Secondary Structure 
SKITKKAVKRGNMRLIQILEAKPKSFSILLSIFTVGLIGLIDYCISPEISVSILYLLPI
CHHHHHHHHCCCHHEEHEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
GIAAWFVNQHTGFFIAVSSDITNLLVNQIQDQYHLHPLIHFWNALVILSFFLFVSHLLSQ
HHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKTTLINLEKLARTDNLTGLTNRGFFLDIVNSEIEKSLRHREPLTLAYFDVDNFKQINDQ
HHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHH
FGHSFGDRLLQIIAKSTTNTLRKIDVIARLGGDEFAILLPRTSYESAEVVLHRLHTVLLA
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
SMKKENLSVTFSIGAITFNNPPKSVAEIIEIADNLMYLAKKKGKNLLQHELCDWKVGSEE
HHCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCC
WKVVNSK
CEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]