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The map label for this gene is nodM [H]

Identifier: 17230956

GI number: 17230956

Start: 4174749

End: 4176374

Strand: Direct

Name: nodM [H]

Synonym: alr3464

Alternate gene names: 17230956

Gene position: 4174749-4176374 (Clockwise)

Preceding gene: 17230955

Following gene: 17230958

Centisome position: 65.09

GC content: 45.26

Gene sequence:

>1626_bases
ATGCGAATCGCTGTTGTGCAGAATGGGATTATTGAAAATTACCGCGAGTTACGCGACGAACTGAAACAGAAAGGACATGA
GTTTCGTTCGGAAACTGATACAGAAGTTATTCCTCACCTTATAGCCGAATTTTTAAAAAACCTCCCTTCCCCCCCGCTTC
CTACTTCTTCCTCCCCCTTCCTAGAAGCAATACGCCAAGCTGTTAAGCACCTACAAGGGGCATTTGCGATCGCTGTTATC
TCTGCTGACTATCCTGACGAATTGATTGTTGTCCGCCAGCAAGCGCCCCTGGTGATAGGATTCGGGCAAGGGGAGTTCTT
TTGCGCCTCCGACACGCCAGCGATCGTTTCCCATACTCGTGCAGTCTTACCCCTGGAGAATGGCGAAATTGCCCGTCTGA
CACCCCTGGGAGTGGAGATTTATAACTTTGCTGGGGACAGACTGAAAAAACAACCCCGCCTGCTTAACTTGAATCCCACA
ATGGTAGAGAAGCAGGGATTCAAACACTTCATGCTCAAGGAAATTTATGAGCAACCAGGAGTAGTCAGAGCGAGTTTAGA
AGCATACTTTAATCCCGATACTAATACTGATGAATCCTTTACATCACCAGTTAATTTGGGTTTATCTGAAGAAATCTATG
CAGATTTAGAACAAATTCATATTGTCGCCTGTGGTACGAGTTGGCACGCTGCATTGGTAGGAAAGTATTTACTTGAACAA
TTAGCAGGAATCTCCACTCAGGTACATTATGCTTCTGAGTATCGCTATGCCCCATCACCATTAACAGCGAATACATTAAT
TATTGGTGTTACTCAGTCAGGGGAAACTGCTGATACCCTAGCCGCCTTAGCGATGGAGAAAGAACGCCGCCAAGGCAAAG
AAGCCAAATATCAAGCGCGACTCTTGGGGATTACCAATCGCCCAGAAAGCAGCCTTGGTCATCTTGTACCCCATATTATT
AATACCCTGGCAGGAATTGAAATTGGTGTGGCAGCGACCAAAACCTTTGTTGCCCAACTGATGGCGTTTTATGCTCTAGC
TTTGGATTTAGCCTATCATCGCCAAACAGTAGCACCAGATAAACTCGCAGATATTATTCAAGGTTTACGTCAGATACCTA
AGGAAATCGAAGCTACCTTAGAACGTCAGGAAAAACTAACAGAACATCTAGCCCATGAATTTGCAGAAACCAAAGACTTC
ATCTTTTTGGGCAGAGGAATTAACTTCCCTATTGCTTTAGAAGGAGCATTGAAATTAAAAGAAATCAGCTACATTCACGC
AGAAGGTTATCCAGCCGGGGAAATGAAACACGGCCCCATTGCATTATTAGATGCCAAAGTGCCGGTAGTGGCGATCGCCT
ATCCTGGTAGTGTGTATGAAAAAGTGATTTCCAATTCCCAAGAAGCCAAAGCCAGAGATTCCCGCTTAATTGGGGTGACA
CCAGTCAACGATGGCGAAGCGGCGGAAATCTTTAATGATTTACTACCTGTTTCATCTGTGGATGAATTACTATCGCCGAT
CCTCACAGTAGTACCATTGCAACTATTGGCTTATCACATTGCCGCCCGTCGCGGTTTGGATGTGGATCAGCCTCGGAACT
TAGCAAAATCAGTGACTGTGGAATAG

Upstream 100 bases:

>100_bases
ACAACTGGCTACTCCATCTCAAATTGGTATTGGTCATACACGTTGGGCAACTCATGGCAAACCAGAGGAGTATAACGCCC
ATCCCCATTTAGATACAGCT

Downstream 100 bases:

>100_bases
GTTGATACGAATATTTAAGTAAATATTTCTAGAGTAGATAAGATAATTTCTAGGTGTGACTACCATATCACATCTAGAAT
TTTTTTTGGGCATCGCTTAA

Product: glucosamine--fructose-6-phosphate aminotransferase

Products: NA

Alternate protein names: D-fructose-6-phosphate amidotransferase; GFAT; Glucosamine-6-phosphate synthase; Hexosephosphate aminotransferase; L-glutamine-D-fructose-6-phosphate amidotransferase [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MRIAVVQNGIIENYRELRDELKQKGHEFRSETDTEVIPHLIAEFLKNLPSPPLPTSSSPFLEAIRQAVKHLQGAFAIAVI
SADYPDELIVVRQQAPLVIGFGQGEFFCASDTPAIVSHTRAVLPLENGEIARLTPLGVEIYNFAGDRLKKQPRLLNLNPT
MVEKQGFKHFMLKEIYEQPGVVRASLEAYFNPDTNTDESFTSPVNLGLSEEIYADLEQIHIVACGTSWHAALVGKYLLEQ
LAGISTQVHYASEYRYAPSPLTANTLIIGVTQSGETADTLAALAMEKERRQGKEAKYQARLLGITNRPESSLGHLVPHII
NTLAGIEIGVAATKTFVAQLMAFYALALDLAYHRQTVAPDKLADIIQGLRQIPKEIEATLERQEKLTEHLAHEFAETKDF
IFLGRGINFPIALEGALKLKEISYIHAEGYPAGEMKHGPIALLDAKVPVVAIAYPGSVYEKVISNSQEAKARDSRLIGVT
PVNDGEAAEIFNDLLPVSSVDELLSPILTVVPLQLLAYHIAARRGLDVDQPRNLAKSVTVE

Sequences:

>Translated_541_residues
MRIAVVQNGIIENYRELRDELKQKGHEFRSETDTEVIPHLIAEFLKNLPSPPLPTSSSPFLEAIRQAVKHLQGAFAIAVI
SADYPDELIVVRQQAPLVIGFGQGEFFCASDTPAIVSHTRAVLPLENGEIARLTPLGVEIYNFAGDRLKKQPRLLNLNPT
MVEKQGFKHFMLKEIYEQPGVVRASLEAYFNPDTNTDESFTSPVNLGLSEEIYADLEQIHIVACGTSWHAALVGKYLLEQ
LAGISTQVHYASEYRYAPSPLTANTLIIGVTQSGETADTLAALAMEKERRQGKEAKYQARLLGITNRPESSLGHLVPHII
NTLAGIEIGVAATKTFVAQLMAFYALALDLAYHRQTVAPDKLADIIQGLRQIPKEIEATLERQEKLTEHLAHEFAETKDF
IFLGRGINFPIALEGALKLKEISYIHAEGYPAGEMKHGPIALLDAKVPVVAIAYPGSVYEKVISNSQEAKARDSRLIGVT
PVNDGEAAEIFNDLLPVSSVDELLSPILTVVPLQLLAYHIAARRGLDVDQPRNLAKSVTVE
>Mature_541_residues
MRIAVVQNGIIENYRELRDELKQKGHEFRSETDTEVIPHLIAEFLKNLPSPPLPTSSSPFLEAIRQAVKHLQGAFAIAVI
SADYPDELIVVRQQAPLVIGFGQGEFFCASDTPAIVSHTRAVLPLENGEIARLTPLGVEIYNFAGDRLKKQPRLLNLNPT
MVEKQGFKHFMLKEIYEQPGVVRASLEAYFNPDTNTDESFTSPVNLGLSEEIYADLEQIHIVACGTSWHAALVGKYLLEQ
LAGISTQVHYASEYRYAPSPLTANTLIIGVTQSGETADTLAALAMEKERRQGKEAKYQARLLGITNRPESSLGHLVPHII
NTLAGIEIGVAATKTFVAQLMAFYALALDLAYHRQTVAPDKLADIIQGLRQIPKEIEATLERQEKLTEHLAHEFAETKDF
IFLGRGINFPIALEGALKLKEISYIHAEGYPAGEMKHGPIALLDAKVPVVAIAYPGSVYEKVISNSQEAKARDSRLIGVT
PVNDGEAAEIFNDLLPVSSVDELLSPILTVVPLQLLAYHIAARRGLDVDQPRNLAKSVTVE

Specific function: Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source [H]

COG id: COG0449

COG function: function code M; Glucosamine 6-phosphate synthetase, contains amidotransferase and phosphosugar isomerase domains

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 SIS domains [H]

Homologues:

Organism=Homo sapiens, GI4826742, Length=588, Percent_Identity=33.6734693877551, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI205277386, Length=584, Percent_Identity=33.0479452054795, Blast_Score=298, Evalue=8e-81,
Organism=Escherichia coli, GI1790167, Length=539, Percent_Identity=43.7847866419295, Blast_Score=398, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17532899, Length=447, Percent_Identity=36.0178970917226, Blast_Score=253, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17532897, Length=446, Percent_Identity=35.8744394618834, Blast_Score=253, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17539970, Length=446, Percent_Identity=36.0986547085202, Blast_Score=243, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6322745, Length=447, Percent_Identity=35.5704697986577, Blast_Score=254, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6323731, Length=451, Percent_Identity=27.4944567627494, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI21357745, Length=591, Percent_Identity=34.1793570219966, Blast_Score=316, Evalue=3e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR005855
- InterPro:   IPR001347 [H]

Pfam domain/function: PF00310 GATase_2; PF01380 SIS [H]

EC number: =2.6.1.16 [H]

Molecular weight: Translated: 59497; Mature: 59497

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAVVQNGIIENYRELRDELKQKGHEFRSETDTEVIPHLIAEFLKNLPSPPLPTSSSPF
CEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHH
LEAIRQAVKHLQGAFAIAVISADYPDELIVVRQQAPLVIGFGQGEFFCASDTPAIVSHTR
HHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEECCCEEEEECCCCEEECCCCCHHHHCCE
AVLPLENGEIARLTPLGVEIYNFAGDRLKKQPRLLNLNPTMVEKQGFKHFMLKEIYEQPG
EEEEECCCCEEEEEECCEEHHHCCCHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHCCC
VVRASLEAYFNPDTNTDESFTSPVNLGLSEEIYADLEQIHIVACGTSWHAALVGKYLLEQ
CEEEEHHHEECCCCCCCCCCCCCCCCCCCHHHHHHHHHEEEEEECCCHHHHHHHHHHHHH
LAGISTQVHYASEYRYAPSPLTANTLIIGVTQSGETADTLAALAMEKERRQGKEAKYQAR
HHCCCCEEEEHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHH
LLGITNRPESSLGHLVPHIINTLAGIEIGVAATKTFVAQLMAFYALALDLAYHRQTVAPD
EEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
KLADIIQGLRQIPKEIEATLERQEKLTEHLAHEFAETKDFIFLGRGINFPIALEGALKLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEECCCEEEH
EISYIHAEGYPAGEMKHGPIALLDAKVPVVAIAYPGSVYEKVISNSQEAKARDSRLIGVT
HEEEEECCCCCCCCCCCCCEEEEECCCCEEEEECCHHHHHHHHCCCHHHHHHCCEEEEEE
PVNDGEAAEIFNDLLPVSSVDELLSPILTVVPLQLLAYHIAARRGLDVDQPRNLAKSVTV
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCC
E
C
>Mature Secondary Structure
MRIAVVQNGIIENYRELRDELKQKGHEFRSETDTEVIPHLIAEFLKNLPSPPLPTSSSPF
CEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHH
LEAIRQAVKHLQGAFAIAVISADYPDELIVVRQQAPLVIGFGQGEFFCASDTPAIVSHTR
HHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEECCCEEEEECCCCEEECCCCCHHHHCCE
AVLPLENGEIARLTPLGVEIYNFAGDRLKKQPRLLNLNPTMVEKQGFKHFMLKEIYEQPG
EEEEECCCCEEEEEECCEEHHHCCCHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHCCC
VVRASLEAYFNPDTNTDESFTSPVNLGLSEEIYADLEQIHIVACGTSWHAALVGKYLLEQ
CEEEEHHHEECCCCCCCCCCCCCCCCCCCHHHHHHHHHEEEEEECCCHHHHHHHHHHHHH
LAGISTQVHYASEYRYAPSPLTANTLIIGVTQSGETADTLAALAMEKERRQGKEAKYQAR
HHCCCCEEEEHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHH
LLGITNRPESSLGHLVPHIINTLAGIEIGVAATKTFVAQLMAFYALALDLAYHRQTVAPD
EEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
KLADIIQGLRQIPKEIEATLERQEKLTEHLAHEFAETKDFIFLGRGINFPIALEGALKLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEECCCEEEH
EISYIHAEGYPAGEMKHGPIALLDAKVPVVAIAYPGSVYEKVISNSQEAKARDSRLIGVT
HEEEEECCCCCCCCCCCCCEEEEECCCCEEEEECCHHHHHHHHCCCHHHHHHCCEEEEEE
PVNDGEAAEIFNDLLPVSSVDELLSPILTVVPLQLLAYHIAARRGLDVDQPRNLAKSVTV
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA