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The map label for this gene is pilT [H]

Identifier: 17230821

GI number: 17230821

Start: 4023220

End: 4024518

Strand: Reverse

Name: pilT [H]

Synonym: all3329

Alternate gene names: 17230821

Gene position: 4024518-4023220 (Counterclockwise)

Preceding gene: 17230824

Following gene: 17230820

Centisome position: 62.75

GC content: 44.42

Gene sequence:

>1299_bases
ATGACGGAATCCCAGTCCCCATCAACTTCCCATCCTGTTGCTTCACGTAATATACCGCCTGTACCACCGCCACCATTACC
ACCACCACCACCATCGTTAACTACCCAACGCCAACATACACAAACGTTGGATATGTCATCAGTTAATAGGAGTGCAAATC
AACCTTCACCGCCTCCACCACCTAGTGCGGCTCATCGTCCTGGGACACCGCCACCAATTTCAACCCCAGCTAGTGATTCG
CCTTTAACTTTGGCGCAGATTATTAAAGAAGCTTTTGATAATGGCTATTCTGATATTCATTTGGGTGTCGGTGAAATTCC
CCGCTACCGCAACCGAGGTGAGATTAACCCAACTGAATATCCCGAAACCGATAAGGCGACTTTTATGAGTTGGTTGCGGG
AAATCATGACTGAGCCAGAAATTCAACGTTTTCAAGAGCATTTGGAATTTGATGGTGCAACTCAGTACGAATTTGCCCGT
GTACGGATTAATGTCTTTGATTCTCTCAAAGGCTATTCGATGGTTTTGCGGTTGATTCCGTTGAAAATCTTATCTATTGA
CCAGTTGAGACTACCACCAGTATTTCGAGATGTTTGTCACACACACAAAGGTTTAATTTTGGTGACAGGCCCGACTGGTT
CTGGTAAATCTACAACAATGGCGGCAATGATTGATTACATCAATCGAGAAATGCCTAAGCACATTATTACGATTGAAGAC
CCTGTGGAATTCGTTCACCAAAGCCGCAAGTCTTTGATTAAGCAACGGGAAGTGGGAATGCACACCCGTAAGTTTGATAA
TGCTTTAAAAGCGGCTTTGCGGGAAGATCCAGATTTGATTCTGGTGGGGGAAATGCGGGATAAAGAAACCGTCAATACAG
CACTGAAAGCGGCTCAAACTGGTCACTTGGTAATGGGAACCCTACACACCAATAGTGCAGTGAAAACTATTGAACGGATT
CTCAATTTATATTCAGGTGAAGAACAGGATGCTATGCGAATAGCCATAGCAGAGTCTTTGGTAGCGGTGATTGCTCAAGG
ATTGTGTCGCACAACTGATGGTAAGCGGGCAGCTTTCCACGATATTTTGATCAACACTGAAGCGATTAAGGAATGGATTA
AGGATGGTAAATATGATGAAATTAGCGAACTGATGAAGCAAGCTAGTTTTGATGGCATGGTTACGATGAATCAATCTTTG
CTCAATCTCTATCAAGAAGGCCGCATCACTGAAGAAACTGCGCTGGAAATGTCACCAACTCCTAACGAAATGGCTCAGTT
CCTCCGAGGACGAGTTTAG

Upstream 100 bases:

>100_bases
TAGCAATCAGGTATCTGCAATGCCGCGTTCCAAAGCTATGAGAACCTTGACCAGAGTTTTTTGCTGTTTCTAGTTAATAA
CTACAAAATCATAAAGATAT

Downstream 100 bases:

>100_bases
ATACGGTAAAAGGTAGAGGGTTAGGACTTACGCAACTGGTAAACTCAATGTCATTAGTCATTAGTTAAGGTTTTGACTTT
GGACTATTGACAGCCTTAAT

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MTESQSPSTSHPVASRNIPPVPPPPLPPPPPSLTTQRQHTQTLDMSSVNRSANQPSPPPPPSAAHRPGTPPPISTPASDS
PLTLAQIIKEAFDNGYSDIHLGVGEIPRYRNRGEINPTEYPETDKATFMSWLREIMTEPEIQRFQEHLEFDGATQYEFAR
VRINVFDSLKGYSMVLRLIPLKILSIDQLRLPPVFRDVCHTHKGLILVTGPTGSGKSTTMAAMIDYINREMPKHIITIED
PVEFVHQSRKSLIKQREVGMHTRKFDNALKAALREDPDLILVGEMRDKETVNTALKAAQTGHLVMGTLHTNSAVKTIERI
LNLYSGEEQDAMRIAIAESLVAVIAQGLCRTTDGKRAAFHDILINTEAIKEWIKDGKYDEISELMKQASFDGMVTMNQSL
LNLYQEGRITEETALEMSPTPNEMAQFLRGRV

Sequences:

>Translated_432_residues
MTESQSPSTSHPVASRNIPPVPPPPLPPPPPSLTTQRQHTQTLDMSSVNRSANQPSPPPPPSAAHRPGTPPPISTPASDS
PLTLAQIIKEAFDNGYSDIHLGVGEIPRYRNRGEINPTEYPETDKATFMSWLREIMTEPEIQRFQEHLEFDGATQYEFAR
VRINVFDSLKGYSMVLRLIPLKILSIDQLRLPPVFRDVCHTHKGLILVTGPTGSGKSTTMAAMIDYINREMPKHIITIED
PVEFVHQSRKSLIKQREVGMHTRKFDNALKAALREDPDLILVGEMRDKETVNTALKAAQTGHLVMGTLHTNSAVKTIERI
LNLYSGEEQDAMRIAIAESLVAVIAQGLCRTTDGKRAAFHDILINTEAIKEWIKDGKYDEISELMKQASFDGMVTMNQSL
LNLYQEGRITEETALEMSPTPNEMAQFLRGRV
>Mature_431_residues
TESQSPSTSHPVASRNIPPVPPPPLPPPPPSLTTQRQHTQTLDMSSVNRSANQPSPPPPPSAAHRPGTPPPISTPASDSP
LTLAQIIKEAFDNGYSDIHLGVGEIPRYRNRGEINPTEYPETDKATFMSWLREIMTEPEIQRFQEHLEFDGATQYEFARV
RINVFDSLKGYSMVLRLIPLKILSIDQLRLPPVFRDVCHTHKGLILVTGPTGSGKSTTMAAMIDYINREMPKHIITIEDP
VEFVHQSRKSLIKQREVGMHTRKFDNALKAALREDPDLILVGEMRDKETVNTALKAAQTGHLVMGTLHTNSAVKTIERIL
NLYSGEEQDAMRIAIAESLVAVIAQGLCRTTDGKRAAFHDILINTEAIKEWIKDGKYDEISELMKQASFDGMVTMNQSLL
NLYQEGRITEETALEMSPTPNEMAQFLRGRV

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=331, Percent_Identity=39.2749244712991, Blast_Score=238, Evalue=4e-64,
Organism=Escherichia coli, GI1789723, Length=278, Percent_Identity=34.1726618705036, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1786296, Length=284, Percent_Identity=32.7464788732394, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 48211; Mature: 48080

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESQSPSTSHPVASRNIPPVPPPPLPPPPPSLTTQRQHTQTLDMSSVNRSANQPSPPPP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCHHHHHCCCCCCCCCCC
PSAAHRPGTPPPISTPASDSPLTLAQIIKEAFDNGYSDIHLGVGEIPRYRNRGEINPTEY
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCHHCCCCCCCCCCCCCCCC
PETDKATFMSWLREIMTEPEIQRFQEHLEFDGATQYEFARVRINVFDSLKGYSMVLRLIP
CCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHH
LKILSIDQLRLPPVFRDVCHTHKGLILVTGPTGSGKSTTMAAMIDYINREMPKHIITIED
HHHHCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEECH
PVEFVHQSRKSLIKQREVGMHTRKFDNALKAALREDPDLILVGEMRDKETVNTALKAAQT
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCC
GHLVMGTLHTNSAVKTIERILNLYSGEEQDAMRIAIAESLVAVIAQGLCRTTDGKRAAFH
CCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
DILINTEAIKEWIKDGKYDEISELMKQASFDGMVTMNQSLLNLYQEGRITEETALEMSPT
HHHHCHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCHHHHCCCCCC
PNEMAQFLRGRV
HHHHHHHHHCCC
>Mature Secondary Structure 
TESQSPSTSHPVASRNIPPVPPPPLPPPPPSLTTQRQHTQTLDMSSVNRSANQPSPPPP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCHHHHHCCCCCCCCCCC
PSAAHRPGTPPPISTPASDSPLTLAQIIKEAFDNGYSDIHLGVGEIPRYRNRGEINPTEY
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCHHCCCCCCCCCCCCCCCC
PETDKATFMSWLREIMTEPEIQRFQEHLEFDGATQYEFARVRINVFDSLKGYSMVLRLIP
CCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHH
LKILSIDQLRLPPVFRDVCHTHKGLILVTGPTGSGKSTTMAAMIDYINREMPKHIITIED
HHHHCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEECH
PVEFVHQSRKSLIKQREVGMHTRKFDNALKAALREDPDLILVGEMRDKETVNTALKAAQT
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCC
GHLVMGTLHTNSAVKTIERILNLYSGEEQDAMRIAIAESLVAVIAQGLCRTTDGKRAAFH
CCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
DILINTEAIKEWIKDGKYDEISELMKQASFDGMVTMNQSLLNLYQEGRITEETALEMSPT
HHHHCHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCHHHHCCCCCC
PNEMAQFLRGRV
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8100347 [H]