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The map label for this gene is metK [H]

Identifier: 17230736

GI number: 17230736

Start: 3918154

End: 3919338

Strand: Reverse

Name: metK [H]

Synonym: all3244

Alternate gene names: 17230736

Gene position: 3919338-3918154 (Counterclockwise)

Preceding gene: 17230737

Following gene: 17230731

Centisome position: 61.11

GC content: 37.13

Gene sequence:

>1185_bases
ATGAAAAAAGATTTCATGTTTACATCTGAATCTGTAACTGAGGGGCATCCTGATAAACTGTGTGATCAGATTAGTGATGC
GATCGTTGATCGCTTTTTACAACAAGACCCCTACGCTAGAGTCATTACCGAATGCGCTGCATCCACAGGTATTGTATTTA
TAGCCGCCAGATTTGAACCTAATACTAATGTTGATTTTACAAACATAGCCAGACAAGTTATTGATCAGGTTGGTTATGAA
CAAATGGAATTTAACGGTAAAAATTGTAGTATATTGACCAGCCTAAAAGAATTGCCTCCTGATCCATATCACTTATTTAA
TGAACACAAACTATCAGATGCAGAAATAGAACAAATTACTGTTACCAACCAAGCCACAGTCTTCGGCTTTGCCTGTCATC
AAACCTCTACCCTCATGCCATTACCAATCTGGTTGGCACACAAATTAGCTAGACAAATTAGTGAAGTTAGAAGACAAAAT
CTTTTAAGTTACTTAACTCCTGATGGCAAAACTCAAGTAGGTGTTGAATATAAAAATCGCCTACCCTATAGAATCCATAG
TATTACAGTAATTGCCAGCCAAAATAAACCGGGAAAACCAGACTTAAAGCAATTACAAGATGATATTAGAGAAACCGTAA
TTTATCCTGTATTTGCTGATGAAGAAATTAAACCAGATGAAAAAACTAGAATATTTATTAATCCCGATGGCCCTTTTATA
GTTGGCGGCCCGGCGGTACATTCTGGTTTAACTGGGAGAAAAAACGCCATAGATACCTATGGTGAATATTCCAAACACAG
TGGTTCGGCTTTAAGTGGTAAAGACCCCATCAGAATAGATAGAGTAGGTGCTTATGTCGCCCGTTATGCTGCTAAAAATG
TAGTCGCTGCAAACTTAGCTGATGAATGTGAAGTACAACTAAGTTATTCTATCGGACTGGCTAGACCTGTAAGCGTCCAA
GTAGAAACTTTTGGAACAGGTAAAATCTCAGATGAAGATATCACCGTCTTATTAGAAAAGCACTTTGATTTTCGTTTAGC
AGGTATTCTGAAACAATTTAATTTAAGACATCTACCCTCATTAACTAAAGGGGGATTTTATAGAAAACTAGCGGCTTATG
GTCATGTAGGTAGACAGGATATCGATTTACCTTGGGAAAAAATAGATAAGGTAGGTTTGTTTTAA

Upstream 100 bases:

>100_bases
TCTGATTGTGAATGAAGCAACCAAAAATACTGGCGCAAGCAAGATGATATTCCCCAGTCCCTAATCCCTAGTACCCAATC
CCCAATCCCCAAGGTGAATT

Downstream 100 bases:

>100_bases
AATTTCAGGATATCTGCGGGGATTGGTAATTGGTAATTGGTAATAGTAAAAAACAGACCATTGCTACGCCTTAGGCTATT
CTGACTTGTGGTACTCAGCT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKKDFMFTSESVTEGHPDKLCDQISDAIVDRFLQQDPYARVITECAASTGIVFIAARFEPNTNVDFTNIARQVIDQVGYE
QMEFNGKNCSILTSLKELPPDPYHLFNEHKLSDAEIEQITVTNQATVFGFACHQTSTLMPLPIWLAHKLARQISEVRRQN
LLSYLTPDGKTQVGVEYKNRLPYRIHSITVIASQNKPGKPDLKQLQDDIRETVIYPVFADEEIKPDEKTRIFINPDGPFI
VGGPAVHSGLTGRKNAIDTYGEYSKHSGSALSGKDPIRIDRVGAYVARYAAKNVVAANLADECEVQLSYSIGLARPVSVQ
VETFGTGKISDEDITVLLEKHFDFRLAGILKQFNLRHLPSLTKGGFYRKLAAYGHVGRQDIDLPWEKIDKVGLF

Sequences:

>Translated_394_residues
MKKDFMFTSESVTEGHPDKLCDQISDAIVDRFLQQDPYARVITECAASTGIVFIAARFEPNTNVDFTNIARQVIDQVGYE
QMEFNGKNCSILTSLKELPPDPYHLFNEHKLSDAEIEQITVTNQATVFGFACHQTSTLMPLPIWLAHKLARQISEVRRQN
LLSYLTPDGKTQVGVEYKNRLPYRIHSITVIASQNKPGKPDLKQLQDDIRETVIYPVFADEEIKPDEKTRIFINPDGPFI
VGGPAVHSGLTGRKNAIDTYGEYSKHSGSALSGKDPIRIDRVGAYVARYAAKNVVAANLADECEVQLSYSIGLARPVSVQ
VETFGTGKISDEDITVLLEKHFDFRLAGILKQFNLRHLPSLTKGGFYRKLAAYGHVGRQDIDLPWEKIDKVGLF
>Mature_394_residues
MKKDFMFTSESVTEGHPDKLCDQISDAIVDRFLQQDPYARVITECAASTGIVFIAARFEPNTNVDFTNIARQVIDQVGYE
QMEFNGKNCSILTSLKELPPDPYHLFNEHKLSDAEIEQITVTNQATVFGFACHQTSTLMPLPIWLAHKLARQISEVRRQN
LLSYLTPDGKTQVGVEYKNRLPYRIHSITVIASQNKPGKPDLKQLQDDIRETVIYPVFADEEIKPDEKTRIFINPDGPFI
VGGPAVHSGLTGRKNAIDTYGEYSKHSGSALSGKDPIRIDRVGAYVARYAAKNVVAANLADECEVQLSYSIGLARPVSVQ
VETFGTGKISDEDITVLLEKHFDFRLAGILKQFNLRHLPSLTKGGFYRKLAAYGHVGRQDIDLPWEKIDKVGLF

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=391, Percent_Identity=39.386189258312, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI4557737, Length=388, Percent_Identity=38.9175257731959, Blast_Score=269, Evalue=4e-72,
Organism=Escherichia coli, GI1789311, Length=391, Percent_Identity=42.1994884910486, Blast_Score=317, Evalue=8e-88,
Organism=Caenorhabditis elegans, GI17538498, Length=389, Percent_Identity=40.6169665809769, Blast_Score=282, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI25145633, Length=400, Percent_Identity=39.25, Blast_Score=276, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17551082, Length=393, Percent_Identity=39.1857506361323, Blast_Score=276, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17538494, Length=389, Percent_Identity=39.5886889460154, Blast_Score=274, Evalue=5e-74,
Organism=Caenorhabditis elegans, GI32565905, Length=391, Percent_Identity=37.0843989769821, Blast_Score=234, Evalue=5e-62,
Organism=Caenorhabditis elegans, GI32565911, Length=343, Percent_Identity=37.0262390670554, Blast_Score=215, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI25145635, Length=351, Percent_Identity=35.8974358974359, Blast_Score=208, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI32565903, Length=312, Percent_Identity=37.5, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI32565909, Length=84, Percent_Identity=46.4285714285714, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323209, Length=395, Percent_Identity=40.253164556962, Blast_Score=276, Evalue=4e-75,
Organism=Saccharomyces cerevisiae, GI6320710, Length=397, Percent_Identity=39.5465994962217, Blast_Score=273, Evalue=3e-74,
Organism=Drosophila melanogaster, GI45552159, Length=389, Percent_Identity=39.3316195372751, Blast_Score=261, Evalue=4e-70,
Organism=Drosophila melanogaster, GI24580529, Length=389, Percent_Identity=39.3316195372751, Blast_Score=261, Evalue=4e-70,
Organism=Drosophila melanogaster, GI24580537, Length=389, Percent_Identity=39.3316195372751, Blast_Score=261, Evalue=4e-70,
Organism=Drosophila melanogaster, GI24580533, Length=389, Percent_Identity=39.3316195372751, Blast_Score=261, Evalue=4e-70,
Organism=Drosophila melanogaster, GI24580535, Length=389, Percent_Identity=39.3316195372751, Blast_Score=261, Evalue=4e-70,
Organism=Drosophila melanogaster, GI24580531, Length=389, Percent_Identity=39.3316195372751, Blast_Score=261, Evalue=4e-70,
Organism=Drosophila melanogaster, GI24580527, Length=389, Percent_Identity=38.3033419023136, Blast_Score=258, Evalue=6e-69,
Organism=Drosophila melanogaster, GI24580525, Length=389, Percent_Identity=38.3033419023136, Blast_Score=258, Evalue=6e-69,
Organism=Drosophila melanogaster, GI24580541, Length=390, Percent_Identity=36.1538461538462, Blast_Score=234, Evalue=6e-62,
Organism=Drosophila melanogaster, GI24580539, Length=99, Percent_Identity=41.4141414141414, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 44118; Mature: 44118

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKDFMFTSESVTEGHPDKLCDQISDAIVDRFLQQDPYARVITECAASTGIVFIAARFEP
CCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEEECC
NTNVDFTNIARQVIDQVGYEQMEFNGKNCSILTSLKELPPDPYHLFNEHKLSDAEIEQIT
CCCCCHHHHHHHHHHHCCCCEEECCCCCCCEEHHHHHCCCCHHHHCCCCCCCCCCEEEEE
VTNQATVFGFACHQTSTLMPLPIWLAHKLARQISEVRRQNLLSYLTPDGKTQVGVEYKNR
EECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECEEHHCC
LPYRIHSITVIASQNKPGKPDLKQLQDDIRETVIYPVFADEEIKPDEKTRIFINPDGPFI
CCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCCCCEEEEECCCCCEE
VGGPAVHSGLTGRKNAIDTYGEYSKHSGSALSGKDPIRIDRVGAYVARYAAKNVVAANLA
ECCHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCC
DECEVQLSYSIGLARPVSVQVETFGTGKISDEDITVLLEKHFDFRLAGILKQFNLRHLPS
CCCEEEEEEEECCCCCEEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHCHHHCCC
LTKGGFYRKLAAYGHVGRQDIDLPWEKIDKVGLF
CCCCCHHHHHHHHCCCCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure
MKKDFMFTSESVTEGHPDKLCDQISDAIVDRFLQQDPYARVITECAASTGIVFIAARFEP
CCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEEECC
NTNVDFTNIARQVIDQVGYEQMEFNGKNCSILTSLKELPPDPYHLFNEHKLSDAEIEQIT
CCCCCHHHHHHHHHHHCCCCEEECCCCCCCEEHHHHHCCCCHHHHCCCCCCCCCCEEEEE
VTNQATVFGFACHQTSTLMPLPIWLAHKLARQISEVRRQNLLSYLTPDGKTQVGVEYKNR
EECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECEEHHCC
LPYRIHSITVIASQNKPGKPDLKQLQDDIRETVIYPVFADEEIKPDEKTRIFINPDGPFI
CCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCCCCEEEEECCCCCEE
VGGPAVHSGLTGRKNAIDTYGEYSKHSGSALSGKDPIRIDRVGAYVARYAAKNVVAANLA
ECCHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCC
DECEVQLSYSIGLARPVSVQVETFGTGKISDEDITVLLEKHFDFRLAGILKQFNLRHLPS
CCCEEEEEEEECCCCCEEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHCHHHCCC
LTKGGFYRKLAAYGHVGRQDIDLPWEKIDKVGLF
CCCCCHHHHHHHHCCCCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA