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The map label for this gene is rihA [H]

Identifier: 17230694

GI number: 17230694

Start: 3868874

End: 3869743

Strand: Direct

Name: rihA [H]

Synonym: alr3202

Alternate gene names: 17230694

Gene position: 3868874-3869743 (Clockwise)

Preceding gene: 17230693

Following gene: 17230695

Centisome position: 60.32

GC content: 46.32

Gene sequence:

>870_bases
ATGGCGCATCATTGGCCGGACGTTGAAATTGTGGCGGTAACAATCGTCAACGGAAATGTCCCCGTTGAGCAAGGAGTCAA
AAACGCCTTATACACAATTCAAGTATGCAACGCCTCTACACCTGTATATGTCGGCTGCACCAAGCCAATACTACGGGAAT
CTCTTTATGCTGATTGGTTTCACGGCAAAGATGGTATGGGTAATATGTACTACCCTGAGCCAAAAAGCAAGCCAGAATCA
GCACACGCCACTGATGTAATTATTGACATTATCAAACAATACCCTGGTGAAATTACCCTGGTGACCCTGGGGCCGTTGAC
AAATATTGCTACTGCACTACTCAAAGCGCCGGAGATTGCCCAACTAGTGCAGCGTTGCGTGATTATGGGTGGTGCAGCGA
ATACAGTGGGTAATGTCACACCTGCGGCTGAATATAACATTTGGGTAGACCCAGAAGCCGCTAAGATTGTCTTTCATAGT
GGAATGCCGATGGAAATGGTCGGTTGGGAGTTGAGCCGTCACGATGCGGCGTTGACTTTTGCAGAAGTAGAAACAGTGAT
GAATTTCGGCACGGAAAGGGCGCGTTTGGCGATGGAGTGTAACAGGACAGCCTTGGATGTTGCCATGAGAGAGCAAGGTG
CTGTTGGTCTCACCTTAGCTGATCCTGTGGCGATCGCAGTTGCGCTTGATCCAGATATTGTTACACGTCAAGGAAAATAC
TTCGTCGATGTGGAAATTACGAGCGAACTAACTAGAGGTGCTACAGTCGTTGATGAGTTAGAAGTGCTAAATAAAAGTCC
AAATATGAATGTGATTTGGGCAATAAATGTGATGGGATGGAAGGAAATTTTGTATTGTTGTTTACACTAG

Upstream 100 bases:

>100_bases
CTGCACCCTGCCCCTCTGCCCATTACCCATTCCCCATTCCCCAATGCGTAAGTTTATCATCGACACAGATACCGCCTCAG
ATGATGCTGTAGCCCTCATC

Downstream 100 bases:

>100_bases
TCTGGGTTGGAGTAGGGAAAGATTACACCAAATCTCTTTAATTTTGAATTTTGAATTGTTACTGGAACCTTTGGCAAAGT
GAGGTTAAAATCACAAACAA

Product: inosine-uridine preferring nucleoside hydrolase

Products: cytosine; ribose; uracil [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MAHHWPDVEIVAVTIVNGNVPVEQGVKNALYTIQVCNASTPVYVGCTKPILRESLYADWFHGKDGMGNMYYPEPKSKPES
AHATDVIIDIIKQYPGEITLVTLGPLTNIATALLKAPEIAQLVQRCVIMGGAANTVGNVTPAAEYNIWVDPEAAKIVFHS
GMPMEMVGWELSRHDAALTFAEVETVMNFGTERARLAMECNRTALDVAMREQGAVGLTLADPVAIAVALDPDIVTRQGKY
FVDVEITSELTRGATVVDELEVLNKSPNMNVIWAINVMGWKEILYCCLH

Sequences:

>Translated_289_residues
MAHHWPDVEIVAVTIVNGNVPVEQGVKNALYTIQVCNASTPVYVGCTKPILRESLYADWFHGKDGMGNMYYPEPKSKPES
AHATDVIIDIIKQYPGEITLVTLGPLTNIATALLKAPEIAQLVQRCVIMGGAANTVGNVTPAAEYNIWVDPEAAKIVFHS
GMPMEMVGWELSRHDAALTFAEVETVMNFGTERARLAMECNRTALDVAMREQGAVGLTLADPVAIAVALDPDIVTRQGKY
FVDVEITSELTRGATVVDELEVLNKSPNMNVIWAINVMGWKEILYCCLH
>Mature_288_residues
AHHWPDVEIVAVTIVNGNVPVEQGVKNALYTIQVCNASTPVYVGCTKPILRESLYADWFHGKDGMGNMYYPEPKSKPESA
HATDVIIDIIKQYPGEITLVTLGPLTNIATALLKAPEIAQLVQRCVIMGGAANTVGNVTPAAEYNIWVDPEAAKIVFHSG
MPMEMVGWELSRHDAALTFAEVETVMNFGTERARLAMECNRTALDVAMREQGAVGLTLADPVAIAVALDPDIVTRQGKYF
VDVEITSELTRGATVVDELEVLNKSPNMNVIWAINVMGWKEILYCCLH

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788486, Length=276, Percent_Identity=35.1449275362319, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1786871, Length=281, Percent_Identity=34.8754448398576, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI1786213, Length=283, Percent_Identity=36.7491166077738, Blast_Score=143, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17565698, Length=294, Percent_Identity=36.0544217687075, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32565474, Length=266, Percent_Identity=31.5789473684211, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6320608, Length=161, Percent_Identity=32.9192546583851, Blast_Score=82, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24641839, Length=287, Percent_Identity=28.9198606271777, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24641837, Length=300, Percent_Identity=28, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24583915, Length=297, Percent_Identity=26.9360269360269, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 31596; Mature: 31465

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHHWPDVEIVAVTIVNGNVPVEQGVKNALYTIQVCNASTPVYVGCTKPILRESLYADWF
CCCCCCCEEEEEEEEEECCCCHHHHHCCCEEEEEEECCCCCEEEECCCHHHHHHHHHHHH
HGKDGMGNMYYPEPKSKPESAHATDVIIDIIKQYPGEITLVTLGPLTNIATALLKAPEIA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCHHH
QLVQRCVIMGGAANTVGNVTPAAEYNIWVDPEAAKIVFHSGMPMEMVGWELSRHDAALTF
HHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCHHHHCCCCCCCCCEEHH
AEVETVMNFGTERARLAMECNRTALDVAMREQGAVGLTLADPVAIAVALDPDIVTRQGKY
HHHHHHHHCCCHHHEEEHHCCCHHHHHHHHCCCCEEEEECCCEEEEEEECCCCEECCCCE
FVDVEITSELTRGATVVDELEVLNKSPNMNVIWAINVMGWKEILYCCLH
EEEEEEECHHHCCCHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHCC
>Mature Secondary Structure 
AHHWPDVEIVAVTIVNGNVPVEQGVKNALYTIQVCNASTPVYVGCTKPILRESLYADWF
CCCCCCEEEEEEEEEECCCCHHHHHCCCEEEEEEECCCCCEEEECCCHHHHHHHHHHHH
HGKDGMGNMYYPEPKSKPESAHATDVIIDIIKQYPGEITLVTLGPLTNIATALLKAPEIA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCHHH
QLVQRCVIMGGAANTVGNVTPAAEYNIWVDPEAAKIVFHSGMPMEMVGWELSRHDAALTF
HHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCHHHHCCCCCCCCCEEHH
AEVETVMNFGTERARLAMECNRTALDVAMREQGAVGLTLADPVAIAVALDPDIVTRQGKY
HHHHHHHHCCCHHHEEEHHCCCHHHHHHHHCCCCEEEEECCCEEEEEEECCCCEECCCCE
FVDVEITSELTRGATVVDELEVLNKSPNMNVIWAINVMGWKEILYCCLH
EEEEEEECHHHCCCHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: cytidine; H2O; uridine [C]

Specific reaction: cytidine + H2O = cytosine + ribose uridine + H2O = uracil + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA