Click here to switch to the map view.

The map label for this gene is cca [H]

Identifier: 17230628

GI number: 17230628

Start: 3794211

End: 3795479

Strand: Reverse

Name: cca [H]

Synonym: all3136

Alternate gene names: 17230628

Gene position: 3795479-3794211 (Counterclockwise)

Preceding gene: 17230633

Following gene: 17230627

Centisome position: 59.18

GC content: 47.36

Gene sequence:

>1269_bases
ATGTCTGGATTTGTTCCTTCTACCCTAGCATCTGAAAATTGGCCTTTTAGCCTGGAATGGTTGCCACAACCTGCTTATAT
GGTTGGTGGTGCGGTGCGGGATGCCATGCTAGGTAGAAGTCGAGAATATCTGGATCTAGATTTTGTGATACCATCGGACG
CTGTGAAAGTAGCTAGAACGATCGCTCATCGTTACCAAGCTGGTTTTGTTTTACTCGATCCGGTGCGGCGAATTGCGCGG
GTGGTATTTCCCCAGGCTACAGCCGACTTTGCTCAACAGGAAGGGGATAGTTTAGAGACTGATCTGCATAGAAGGGATTT
TACAGTTAATGCGATCGCCTACAATCCCCACACCCAAGAAATCATCGACCCCCTCAACGGTTATGCTGACTTACAGGCGG
GTGTGTTGCGGATGATAGCCAAAGCTAACCTCAAAGACGACCCTTTACGTTTATTACGAGCTTACCGCCAAGCCGCTCAA
CTAGGCTTTACTATTGAACCGACAACCCAAGCCGCTATTCGTGATTTAGCATCACATCTGTGTCAAGTCGCCGCCGAACG
AGTGCGAGTAGAAATTGGCTATTTACTTGCTAGTCCTCAAGGTCATACTTGGATGGCAATTGCTGAGAATGATGGTTTAC
TCAAGCAATTCTTCCCAAATACCACACCTGAAAGCTTTCGTAAATTAGCGGTAGTTGATGAAGTCGCTGATTTATTGGCA
AAACAGTGGCCGCAACTAGGTGTGGAACTGCAAGGATACGTCCGGGAAACAGTAAAAACCACTTGGCTAAGTATTGCCAA
ACTCGCTTGTCTAGTCCACCCTGATCCAAGTGTTGCAGAAATCGAGCTGCAAAAACTCACCTACAGTCGCGCCGAGATTC
GCGCTGTAACTACGGCTGTGAAACTATTTCCCCAGCTAAAAACAGCTAATACCTTGCGAGAACAGTACTTTGTGTTTCAA
GAAGCAGGTATGGTATTTGCAGCGATCGCTGTTTTAGCTTTAGTACAAGATATCCTGGTAGAGGCGATGTCTGGCGACAA
TCGGCTATCCGCTTACTCTCCATTGATCACCCGCTACCTTAACCCTGATGATCTGGTCGCTCATCCCACTCAGCTAGTAA
GTGGTAAAAATCTAATGATAGCACTCGATCTTCCCCCCTCGCCACTGGTGGGAGAATTACTAACAGAGATTGCGATCGCC
AGAATTGAGGGGAAAGTGACAACAACAGAAGCAGCGATCGCCTTTGCTAGACAGCTACTTGAAGGTTAG

Upstream 100 bases:

>100_bases
TACGCAAATACACATAATAGAACCAACTCCAAGTCCTTTATTGTTAATCTAGCTTAAGCTAAAGCATTCAGCACCAGTTA
CTGGTTAGAGTTTTATTACA

Downstream 100 bases:

>100_bases
AAATTGGTTTTTCATTGATATATTTCGCAAATTTGGCTATCTTGCCAGGTTTATAAATCTTTACTGATCTGTGAAAAAAT
CACTATTCAAGTCCGGTTAA

Product: polyA polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MSGFVPSTLASENWPFSLEWLPQPAYMVGGAVRDAMLGRSREYLDLDFVIPSDAVKVARTIAHRYQAGFVLLDPVRRIAR
VVFPQATADFAQQEGDSLETDLHRRDFTVNAIAYNPHTQEIIDPLNGYADLQAGVLRMIAKANLKDDPLRLLRAYRQAAQ
LGFTIEPTTQAAIRDLASHLCQVAAERVRVEIGYLLASPQGHTWMAIAENDGLLKQFFPNTTPESFRKLAVVDEVADLLA
KQWPQLGVELQGYVRETVKTTWLSIAKLACLVHPDPSVAEIELQKLTYSRAEIRAVTTAVKLFPQLKTANTLREQYFVFQ
EAGMVFAAIAVLALVQDILVEAMSGDNRLSAYSPLITRYLNPDDLVAHPTQLVSGKNLMIALDLPPSPLVGELLTEIAIA
RIEGKVTTTEAAIAFARQLLEG

Sequences:

>Translated_422_residues
MSGFVPSTLASENWPFSLEWLPQPAYMVGGAVRDAMLGRSREYLDLDFVIPSDAVKVARTIAHRYQAGFVLLDPVRRIAR
VVFPQATADFAQQEGDSLETDLHRRDFTVNAIAYNPHTQEIIDPLNGYADLQAGVLRMIAKANLKDDPLRLLRAYRQAAQ
LGFTIEPTTQAAIRDLASHLCQVAAERVRVEIGYLLASPQGHTWMAIAENDGLLKQFFPNTTPESFRKLAVVDEVADLLA
KQWPQLGVELQGYVRETVKTTWLSIAKLACLVHPDPSVAEIELQKLTYSRAEIRAVTTAVKLFPQLKTANTLREQYFVFQ
EAGMVFAAIAVLALVQDILVEAMSGDNRLSAYSPLITRYLNPDDLVAHPTQLVSGKNLMIALDLPPSPLVGELLTEIAIA
RIEGKVTTTEAAIAFARQLLEG
>Mature_421_residues
SGFVPSTLASENWPFSLEWLPQPAYMVGGAVRDAMLGRSREYLDLDFVIPSDAVKVARTIAHRYQAGFVLLDPVRRIARV
VFPQATADFAQQEGDSLETDLHRRDFTVNAIAYNPHTQEIIDPLNGYADLQAGVLRMIAKANLKDDPLRLLRAYRQAAQL
GFTIEPTTQAAIRDLASHLCQVAAERVRVEIGYLLASPQGHTWMAIAENDGLLKQFFPNTTPESFRKLAVVDEVADLLAK
QWPQLGVELQGYVRETVKTTWLSIAKLACLVHPDPSVAEIELQKLTYSRAEIRAVTTAVKLFPQLKTANTLREQYFVFQE
AGMVFAAIAVLALVQDILVEAMSGDNRLSAYSPLITRYLNPDDLVAHPTQLVSGKNLMIALDLPPSPLVGELLTEIAIAR
IEGKVTTTEAAIAFARQLLEG

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=218, Percent_Identity=33.9449541284404, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87081691, Length=223, Percent_Identity=29.1479820627803, Blast_Score=82, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6321016, Length=107, Percent_Identity=41.1214953271028, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 46646; Mature: 46515

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00287 CYSTATIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFVPSTLASENWPFSLEWLPQPAYMVGGAVRDAMLGRSREYLDLDFVIPSDAVKVART
CCCCCCCHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHH
IAHRYQAGFVLLDPVRRIARVVFPQATADFAQQEGDSLETDLHRRDFTVNAIAYNPHTQE
HHHHHHCCEEEHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHCCEEEEEEEECCCHHH
IIDPLNGYADLQAGVLRMIAKANLKDDPLRLLRAYRQAAQLGFTIEPTTQAAIRDLASHL
HHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHH
CQVAAERVRVEIGYLLASPQGHTWMAIAENDGLLKQFFPNTTPESFRKLAVVDEVADLLA
HHHHHHHHHEEEEEEEECCCCCEEEEEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHH
KQWPQLGVELQGYVRETVKTTWLSIAKLACLVHPDPSVAEIELQKLTYSRAEIRAVTTAV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHEEHHHHHHHHHHHHHHHHHH
KLFPQLKTANTLREQYFVFQEAGMVFAAIAVLALVQDILVEAMSGDNRLSAYSPLITRYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
NPDDLVAHPTQLVSGKNLMIALDLPPSPLVGELLTEIAIARIEGKVTTTEAAIAFARQLL
CCCHHHCCCHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHH
EG
CC
>Mature Secondary Structure 
SGFVPSTLASENWPFSLEWLPQPAYMVGGAVRDAMLGRSREYLDLDFVIPSDAVKVART
CCCCCCHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHH
IAHRYQAGFVLLDPVRRIARVVFPQATADFAQQEGDSLETDLHRRDFTVNAIAYNPHTQE
HHHHHHCCEEEHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHCCEEEEEEEECCCHHH
IIDPLNGYADLQAGVLRMIAKANLKDDPLRLLRAYRQAAQLGFTIEPTTQAAIRDLASHL
HHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHH
CQVAAERVRVEIGYLLASPQGHTWMAIAENDGLLKQFFPNTTPESFRKLAVVDEVADLLA
HHHHHHHHHEEEEEEEECCCCCEEEEEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHH
KQWPQLGVELQGYVRETVKTTWLSIAKLACLVHPDPSVAEIELQKLTYSRAEIRAVTTAV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHEEHHHHHHHHHHHHHHHHHH
KLFPQLKTANTLREQYFVFQEAGMVFAAIAVLALVQDILVEAMSGDNRLSAYSPLITRYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
NPDDLVAHPTQLVSGKNLMIALDLPPSPLVGELLTEIAIARIEGKVTTTEAAIAFARQLL
CCCHHHCCCHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHH
EG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA