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The map label for this gene is nifS [H]

Identifier: 17230580

GI number: 17230580

Start: 3736385

End: 3737548

Strand: Direct

Name: nifS [H]

Synonym: alr3088

Alternate gene names: 17230580

Gene position: 3736385-3737548 (Clockwise)

Preceding gene: 17230578

Following gene: 17230581

Centisome position: 58.26

GC content: 45.45

Gene sequence:

>1164_bases
ATGCAAATATATTTAGATTACAGTGCTACTACTCCCACTCGTGCAGAAGCGATCGCAGTTATGCAATCAGTCCTGACTCA
ACAGTGGGGGAATCCTTCTAGTTTACATGAATGGGGGCAACGGGCAACCACTATTGTAGAACAAGCAAGAGTGCAGGTAG
CAGGGTTAATTAACGCTGCCAATCCCGAATCAATTATTTTTACCTCTGGCGGTACAGAAGCCAACAACCTAGCAATTATG
GGTGTTGTCAGAATGCAAAATGTACCGCAACATATCATTATTTCTAGTGTGGAACATTCGGCTATTAGCGAACCTGCCAA
CTTACTAGAAAAATGGGGTTGGGAAGTCACCCGTCTGGGTGTGGACAATACAGGGAGAGTTAACCCCGAACATTTAAAAG
CTGCATTGCGACATAACACGGTTTTAGTTTCTGTTATTTACGGTCAAAGTGAGATTGGCACAGTCCAACCAATTGCAGAG
TTAGGTAAGATAACTCAACAACATGGGGCGTTGTTCCACACAGATGCAGTGCAAGCAGTGGGACGTTTACCTATAGATGT
GCAGCAACTACCTGTAGATTTACTCAGCCTTTCAAGTCACAAGCTATACGGCCCGCAGGGGGTAGGGGCGCTATATATTC
GTCCTGGTGTGGAATTATTGCCTTTATTGAATGGTGGTGGACAAGAAAGGGGATTACGTTCTGGTACACAAGCAGTACCT
ATCATCGCCGGTTTTGGTGTTGCCGCCGAAATCGCTGCCGAGGAATTATCTATAGAGATACCACGTTTAATTACATTACG
CGATCGCTTATTTGCCCAGTTAGCAGATGTCCCCGGTTTAATCCCCACCGGCGACAGAGAAAGTCGTCTACCCCATCATG
TGAGTTTCTACCTCCAACAAGCCGACGGTGAAAAACTCAGTGGTAAAACCTTAGTGCGTCATTTAAACCTTGCCGGTATT
GGCATTAGTGCGGGCGCAGCTTGTCACAGTGGTAAACTTAGTCCCAGCCCCATATTGTTGGCAATGGGTTACTCAAAAAA
AGCTGCCTTGGGGGGAATTAGGTTGACATTAGGGCGTGAGACTACAGCAGCCGATGTAGATTGGACAGCGATGGTTTTAA
AGCAAGTATTACAGAGATTAATACCAGATGTATCTTTAGTTTAA

Upstream 100 bases:

>100_bases
TGGGATGGGAATGGACATATACATAATAATTCCCACATTTGTGATTAATCCATAGTCAACAGTCAGTATTTATTCCCCTC
GGCCCCTGCCCTTGCCTTAT

Downstream 100 bases:

>100_bases
TTTTGGGCGTTGCTGATTGAAAATATGAATTTGCCTCACACATTCGACGAAGTTGTTCTCGTTCGCGCAGCGTCTCCGAC
AGGAGAAGGAGAAGAGTAGG

Product: class-V aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MQIYLDYSATTPTRAEAIAVMQSVLTQQWGNPSSLHEWGQRATTIVEQARVQVAGLINAANPESIIFTSGGTEANNLAIM
GVVRMQNVPQHIIISSVEHSAISEPANLLEKWGWEVTRLGVDNTGRVNPEHLKAALRHNTVLVSVIYGQSEIGTVQPIAE
LGKITQQHGALFHTDAVQAVGRLPIDVQQLPVDLLSLSSHKLYGPQGVGALYIRPGVELLPLLNGGGQERGLRSGTQAVP
IIAGFGVAAEIAAEELSIEIPRLITLRDRLFAQLADVPGLIPTGDRESRLPHHVSFYLQQADGEKLSGKTLVRHLNLAGI
GISAGAACHSGKLSPSPILLAMGYSKKAALGGIRLTLGRETTAADVDWTAMVLKQVLQRLIPDVSLV

Sequences:

>Translated_387_residues
MQIYLDYSATTPTRAEAIAVMQSVLTQQWGNPSSLHEWGQRATTIVEQARVQVAGLINAANPESIIFTSGGTEANNLAIM
GVVRMQNVPQHIIISSVEHSAISEPANLLEKWGWEVTRLGVDNTGRVNPEHLKAALRHNTVLVSVIYGQSEIGTVQPIAE
LGKITQQHGALFHTDAVQAVGRLPIDVQQLPVDLLSLSSHKLYGPQGVGALYIRPGVELLPLLNGGGQERGLRSGTQAVP
IIAGFGVAAEIAAEELSIEIPRLITLRDRLFAQLADVPGLIPTGDRESRLPHHVSFYLQQADGEKLSGKTLVRHLNLAGI
GISAGAACHSGKLSPSPILLAMGYSKKAALGGIRLTLGRETTAADVDWTAMVLKQVLQRLIPDVSLV
>Mature_387_residues
MQIYLDYSATTPTRAEAIAVMQSVLTQQWGNPSSLHEWGQRATTIVEQARVQVAGLINAANPESIIFTSGGTEANNLAIM
GVVRMQNVPQHIIISSVEHSAISEPANLLEKWGWEVTRLGVDNTGRVNPEHLKAALRHNTVLVSVIYGQSEIGTVQPIAE
LGKITQQHGALFHTDAVQAVGRLPIDVQQLPVDLLSLSSHKLYGPQGVGALYIRPGVELLPLLNGGGQERGLRSGTQAVP
IIAGFGVAAEIAAEELSIEIPRLITLRDRLFAQLADVPGLIPTGDRESRLPHHVSFYLQQADGEKLSGKTLVRHLNLAGI
GISAGAACHSGKLSPSPILLAMGYSKKAALGGIRLTLGRETTAADVDWTAMVLKQVLQRLIPDVSLV

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=383, Percent_Identity=40.2088772845953, Blast_Score=281, Evalue=8e-76,
Organism=Homo sapiens, GI156713448, Length=425, Percent_Identity=36, Blast_Score=223, Evalue=2e-58,
Organism=Escherichia coli, GI48994898, Length=384, Percent_Identity=41.6666666666667, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI1787970, Length=229, Percent_Identity=31.4410480349345, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1789175, Length=403, Percent_Identity=26.0545905707196, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25143064, Length=384, Percent_Identity=41.40625, Blast_Score=276, Evalue=9e-75,
Organism=Caenorhabditis elegans, GI17533177, Length=342, Percent_Identity=30.7017543859649, Blast_Score=144, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6319831, Length=382, Percent_Identity=42.9319371727749, Blast_Score=292, Evalue=7e-80,
Organism=Drosophila melanogaster, GI20129463, Length=383, Percent_Identity=39.686684073107, Blast_Score=271, Evalue=8e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41392; Mature: 41392

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIYLDYSATTPTRAEAIAVMQSVLTQQWGNPSSLHEWGQRATTIVEQARVQVAGLINAA
CEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECC
NPESIIFTSGGTEANNLAIMGVVRMQNVPQHIIISSVEHSAISEPANLLEKWGWEVTRLG
CCCEEEEECCCCCCCCEEEEEEEECCCCCHHHHHCCHHHHHHCCHHHHHHHCCCEEEEEC
VDNTGRVNPEHLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITQQHGALFHTDAVQAV
CCCCCCCCHHHHHHHHHCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEHHHHHHH
GRLPIDVQQLPVDLLSLSSHKLYGPQGVGALYIRPGVELLPLLNGGGQERGLRSGTQAVP
CCCCCCHHHCCHHHHHCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCCCCCE
IIAGFGVAAEIAAEELSIEIPRLITLRDRLFAQLADVPGLIPTGDRESRLPHHVSFYLQQ
EEECCCHHHHHHHHHHCEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHEEEEEC
ADGEKLSGKTLVRHLNLAGIGISAGAACHSGKLSPSPILLAMGYSKKAALGGIRLTLGRE
CCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHCCCEEEEECCC
TTAADVDWTAMVLKQVLQRLIPDVSLV
CCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQIYLDYSATTPTRAEAIAVMQSVLTQQWGNPSSLHEWGQRATTIVEQARVQVAGLINAA
CEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECC
NPESIIFTSGGTEANNLAIMGVVRMQNVPQHIIISSVEHSAISEPANLLEKWGWEVTRLG
CCCEEEEECCCCCCCCEEEEEEEECCCCCHHHHHCCHHHHHHCCHHHHHHHCCCEEEEEC
VDNTGRVNPEHLKAALRHNTVLVSVIYGQSEIGTVQPIAELGKITQQHGALFHTDAVQAV
CCCCCCCCHHHHHHHHHCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEHHHHHHH
GRLPIDVQQLPVDLLSLSSHKLYGPQGVGALYIRPGVELLPLLNGGGQERGLRSGTQAVP
CCCCCCHHHCCHHHHHCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCCCCCE
IIAGFGVAAEIAAEELSIEIPRLITLRDRLFAQLADVPGLIPTGDRESRLPHHVSFYLQQ
EEECCCHHHHHHHHHHCEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHEEEEEC
ADGEKLSGKTLVRHLNLAGIGISAGAACHSGKLSPSPILLAMGYSKKAALGGIRLTLGRE
CCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHCCCEEEEECCC
TTAADVDWTAMVLKQVLQRLIPDVSLV
CCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA