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The map label for this gene is def

Identifier: 17230571

GI number: 17230571

Start: 3729034

End: 3729597

Strand: Direct

Name: def

Synonym: alr3079

Alternate gene names: 17230571

Gene position: 3729034-3729597 (Clockwise)

Preceding gene: 17230570

Following gene: 17230572

Centisome position: 58.14

GC content: 44.68

Gene sequence:

>564_bases
ATGCCTTCTGAAATTGCTGTTGAGAAAAAAAAGTTAAAAAATCCACCTCTGCAACTCCACTATTTAGGCGATCGCGTGCT
GCGTCAACCAGCAAAGCGGATCACGAAAGTAGATGATGAAACCCGGCAATTGATCCGTGATATGCTGCAAACTATGTACA
GCAGTGATGGCATTGGTTTGGCTGCACCCCAGGTGGGAATTAATAAACAACTCATTGTCATCGATTGCGAACCGGATAAC
CCAGCGAATCCGCCACTGATATTAATTAACCCTACAATTAAACAAGTAAGCCGGGAAATTTGTAGCGCGCAGGAAGGGTG
TTTAAGCATTCCAGGGGTTTATATGGATGTCAAACGCCCGGAAGTGGTAGAAGTTGCCTATAAAGATGAAAATGGCCGTC
CTCAGACCTTAAAAGCTACCGACCTACTCGGACGCTGCATTCAACACGAGATGGATCACCTCAACGGTGTAGTATTTGTA
GACCGTGTAGATAATTCCTTGACTTTGGCACAGGAGTTATCTAAAAATGGCTTCTCGTATCAAGCAGTGAAACCAGTAGC
ATAG

Upstream 100 bases:

>100_bases
GAGAAGAAAACTATTGACAACTAACAACTGACAACTGACTAATGACTAAATTGATAGAATTGGGTTAGTAGGAATTTGCA
CAATCTAGCCGACAAAAGTC

Downstream 100 bases:

>100_bases
GGTGGACTAGTAGTGTATTTAACTCCAAAAAGCGGTTTATTTCTCGCTGGCTCTTGCATAAGTGCGATCGCGGCTGTTGG
CTCGGTGTTTGAACTCAGTT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MPSEIAVEKKKLKNPPLQLHYLGDRVLRQPAKRITKVDDETRQLIRDMLQTMYSSDGIGLAAPQVGINKQLIVIDCEPDN
PANPPLILINPTIKQVSREICSAQEGCLSIPGVYMDVKRPEVVEVAYKDENGRPQTLKATDLLGRCIQHEMDHLNGVVFV
DRVDNSLTLAQELSKNGFSYQAVKPVA

Sequences:

>Translated_187_residues
MPSEIAVEKKKLKNPPLQLHYLGDRVLRQPAKRITKVDDETRQLIRDMLQTMYSSDGIGLAAPQVGINKQLIVIDCEPDN
PANPPLILINPTIKQVSREICSAQEGCLSIPGVYMDVKRPEVVEVAYKDENGRPQTLKATDLLGRCIQHEMDHLNGVVFV
DRVDNSLTLAQELSKNGFSYQAVKPVA
>Mature_186_residues
PSEIAVEKKKLKNPPLQLHYLGDRVLRQPAKRITKVDDETRQLIRDMLQTMYSSDGIGLAAPQVGINKQLIVIDCEPDNP
ANPPLILINPTIKQVSREICSAQEGCLSIPGVYMDVKRPEVVEVAYKDENGRPQTLKATDLLGRCIQHEMDHLNGVVFVD
RVDNSLTLAQELSKNGFSYQAVKPVA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=161, Percent_Identity=30.4347826086957, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1789682, Length=145, Percent_Identity=44.1379310344828, Blast_Score=114, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24645728, Length=174, Percent_Identity=31.0344827586207, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24645726, Length=162, Percent_Identity=29.6296296296296, Blast_Score=77, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF1_NOSS1 (Q8YSK6)

Other databases:

- EMBL:   BA000019
- PIR:   AH2190
- RefSeq:   NP_487119.1
- ProteinModelPortal:   Q8YSK6
- SMR:   Q8YSK6
- STRING:   Q8YSK6
- GeneID:   1106677
- GenomeReviews:   BA000019_GR
- KEGG:   ana:alr3079
- NMPDR:   fig|103690.1.peg.3386
- eggNOG:   COG0242
- HOGENOM:   HBG665227
- OMA:   TRASEVT
- PhylomeDB:   Q8YSK6
- ProtClustDB:   PRK00150
- BioCyc:   NSP103690:ALR3079-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 20827; Mature: 20696

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: ACT_SITE 150-150

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSEIAVEKKKLKNPPLQLHYLGDRVLRQPAKRITKVDDETRQLIRDMLQTMYSSDGIGL
CCCCHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCE
AAPQVGINKQLIVIDCEPDNPANPPLILINPTIKQVSREICSAQEGCLSIPGVYMDVKRP
ECCCCCCCCEEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCEEEECCCC
EVVEVAYKDENGRPQTLKATDLLGRCIQHEMDHLNGVVFVDRVDNSLTLAQELSKNGFSY
CEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCCE
QAVKPVA
EEECCCC
>Mature Secondary Structure 
PSEIAVEKKKLKNPPLQLHYLGDRVLRQPAKRITKVDDETRQLIRDMLQTMYSSDGIGL
CCCHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCE
AAPQVGINKQLIVIDCEPDNPANPPLILINPTIKQVSREICSAQEGCLSIPGVYMDVKRP
ECCCCCCCCEEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCEEEECCCC
EVVEVAYKDENGRPQTLKATDLLGRCIQHEMDHLNGVVFVDRVDNSLTLAQELSKNGFSY
CEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCCE
QAVKPVA
EEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840