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The map label for this gene is barA [H]

Identifier: 17230529

GI number: 17230529

Start: 3678017

End: 3679207

Strand: Direct

Name: barA [H]

Synonym: alr3037

Alternate gene names: 17230529

Gene position: 3678017-3679207 (Clockwise)

Preceding gene: 17230519

Following gene: 17230530

Centisome position: 57.35

GC content: 38.79

Gene sequence:

>1191_bases
ATGGAATTTAGTCAAGTACTGGCTGACAATACTAACATCATCATCGCCAAATGGGTTGTGGCAGTGCGTGAGGATAGACA
GATAGAAAGCGCTGATGATTTATCATACACAGCCATTAAAAACCATATTCCTGATGTTTTTAAGGCAATGGTCACAGTAC
TTTCACAATCCCAAGAGAGTGATATTAAATCCATTGTGACGGCCAGTTGGCAGCACGGATTACTACGGGCTGAACAGGGT
TTTGATCCGTCAGAAATTGCCCGCGAATATAGACTATTAAGGAAAGTTGTATTTGATACTATAGAAGTTGATTTATTACA
GAGAACACCCGGCGAAATTATTCGTTCTATGCGTTTAATTGATGCGGTGATTGATGAAGCGATCGCCAGATGTTTTCATA
GTTATGTTGGTGAACGCTTGCGGGAATTAGAACATTTGCAATCTTCTTTGACGCTTCATAATGAAGAACTAACACGTTTA
GTCACTAGCAACGAAGATAAACTATCACAACTAGCCCACGAACTTAAACACCCACTCACGGCGATTATTGGTTATTCAGA
TTTATTTTTACGACAACAAAGGCGAGAAATTACAAGAATAGATAACACGGTCAACATAGAACATATTGAGCGCGTATTAC
GCAATGGCAGACATTTATTGCGGTTAATTAATAATGTCCTAGAACTGTCACGATATGAAGCCGGTAAAATTCAGTTGCAA
CCGGCACCAATTCATATTCGAGACTTAATTAACAATGTTTGTGAGATGCTAGAACCTTTAGCGGCGGAGAAGAATTTAGC
AATTTTAATTAACTGGGTACAAGCACCTAGTGAAATAATTACAGATGGTTTTCAATTACAACAAATTATTACCAATCTTG
TGAGTAATGCAATTCGCTATACAGAGTCAGGCAACATTAGTATAGTCTGTGAAGCAATAGATGATCAAAAGTGGGCGATC
GCTGTCTCTGATACAGGAATTGGCATTGCCGAAAAAGACCAAACTAAGATATTTGAACCTTATTTTCGCGTTAGTTCTAA
AGACCGTCCCTACCTTCCTGACAGTACAGGCTTAGGTTTAGCAATCGTTTCGCGGTTAGTGAAGCTATTGCAAGGTGAAA
TTAACTTAGTCTCCAAAGAAGGGTTGGGTTCCACATTCACTGTGACTTTACCTTTAAAAGTAAAAACTTGA

Upstream 100 bases:

>100_bases
GTAGGTGAATTTTCTCTCTGATTGCGATAATGTCCACTCTGCTCGTTAAACTAATTTCATATAGAATTAATTCCTCTATC
TCATAGCTGAATTTAAAATA

Downstream 100 bases:

>100_bases
GCAATTGTCAACCAAAAGGGGATGGAATTTTACGCTTGATCAATAAATTTTCCTGCACGAAATTCTCCTGCACGCCGCCT
CAACTTTAAAATAAATGTAG

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MEFSQVLADNTNIIIAKWVVAVREDRQIESADDLSYTAIKNHIPDVFKAMVTVLSQSQESDIKSIVTASWQHGLLRAEQG
FDPSEIAREYRLLRKVVFDTIEVDLLQRTPGEIIRSMRLIDAVIDEAIARCFHSYVGERLRELEHLQSSLTLHNEELTRL
VTSNEDKLSQLAHELKHPLTAIIGYSDLFLRQQRREITRIDNTVNIEHIERVLRNGRHLLRLINNVLELSRYEAGKIQLQ
PAPIHIRDLINNVCEMLEPLAAEKNLAILINWVQAPSEIITDGFQLQQIITNLVSNAIRYTESGNISIVCEAIDDQKWAI
AVSDTGIGIAEKDQTKIFEPYFRVSSKDRPYLPDSTGLGLAIVSRLVKLLQGEINLVSKEGLGSTFTVTLPLKVKT

Sequences:

>Translated_396_residues
MEFSQVLADNTNIIIAKWVVAVREDRQIESADDLSYTAIKNHIPDVFKAMVTVLSQSQESDIKSIVTASWQHGLLRAEQG
FDPSEIAREYRLLRKVVFDTIEVDLLQRTPGEIIRSMRLIDAVIDEAIARCFHSYVGERLRELEHLQSSLTLHNEELTRL
VTSNEDKLSQLAHELKHPLTAIIGYSDLFLRQQRREITRIDNTVNIEHIERVLRNGRHLLRLINNVLELSRYEAGKIQLQ
PAPIHIRDLINNVCEMLEPLAAEKNLAILINWVQAPSEIITDGFQLQQIITNLVSNAIRYTESGNISIVCEAIDDQKWAI
AVSDTGIGIAEKDQTKIFEPYFRVSSKDRPYLPDSTGLGLAIVSRLVKLLQGEINLVSKEGLGSTFTVTLPLKVKT
>Mature_396_residues
MEFSQVLADNTNIIIAKWVVAVREDRQIESADDLSYTAIKNHIPDVFKAMVTVLSQSQESDIKSIVTASWQHGLLRAEQG
FDPSEIAREYRLLRKVVFDTIEVDLLQRTPGEIIRSMRLIDAVIDEAIARCFHSYVGERLRELEHLQSSLTLHNEELTRL
VTSNEDKLSQLAHELKHPLTAIIGYSDLFLRQQRREITRIDNTVNIEHIERVLRNGRHLLRLINNVLELSRYEAGKIQLQ
PAPIHIRDLINNVCEMLEPLAAEKNLAILINWVQAPSEIITDGFQLQQIITNLVSNAIRYTESGNISIVCEAIDDQKWAI
AVSDTGIGIAEKDQTKIFEPYFRVSSKDRPYLPDSTGLGLAIVSRLVKLLQGEINLVSKEGLGSTFTVTLPLKVKT

Specific function: Member of the two-component regulatory system UvrY/BarA involved in the regulation of carbon metabolism via the CsrA/CsrB regulatory system. Phosphorylates UvrY, probably via a four-step phosphorelay [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=224, Percent_Identity=37.9464285714286, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI145693157, Length=272, Percent_Identity=31.9852941176471, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1788713, Length=236, Percent_Identity=29.6610169491525, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI48994928, Length=225, Percent_Identity=29.7777777777778, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI87081816, Length=342, Percent_Identity=28.0701754385965, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1788549, Length=251, Percent_Identity=29.8804780876494, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1786783, Length=253, Percent_Identity=25.296442687747, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1790436, Length=225, Percent_Identity=28, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786912, Length=248, Percent_Identity=26.2096774193548, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790346, Length=241, Percent_Identity=27.3858921161826, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082128, Length=247, Percent_Identity=26.3157894736842, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788279, Length=260, Percent_Identity=28.0769230769231, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1788393, Length=261, Percent_Identity=23.7547892720307, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790861, Length=215, Percent_Identity=28.8372093023256, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786600, Length=244, Percent_Identity=26.2295081967213, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR019247
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF09984 DUF2222; PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 44858; Mature: 44858

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFSQVLADNTNIIIAKWVVAVREDRQIESADDLSYTAIKNHIPDVFKAMVTVLSQSQES
CCHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHH
DIKSIVTASWQHGLLRAEQGFDPSEIAREYRLLRKVVFDTIEVDLLQRTPGEIIRSMRLI
HHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
DAVIDEAIARCFHSYVGERLRELEHLQSSLTLHNEELTRLVTSNEDKLSQLAHELKHPLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AIIGYSDLFLRQQRREITRIDNTVNIEHIERVLRNGRHLLRLINNVLELSRYEAGKIQLQ
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEC
PAPIHIRDLINNVCEMLEPLAAEKNLAILINWVQAPSEIITDGFQLQQIITNLVSNAIRY
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHCCHHHHHCHHHHHHHHHHHHHHHHHC
TESGNISIVCEAIDDQKWAIAVSDTGIGIAEKDQTKIFEPYFRVSSKDRPYLPDSTGLGL
CCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHH
AIVSRLVKLLQGEINLVSKEGLGSTFTVTLPLKVKT
HHHHHHHHHHHCHHHEEECCCCCCEEEEEEEEEECC
>Mature Secondary Structure
MEFSQVLADNTNIIIAKWVVAVREDRQIESADDLSYTAIKNHIPDVFKAMVTVLSQSQES
CCHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHH
DIKSIVTASWQHGLLRAEQGFDPSEIAREYRLLRKVVFDTIEVDLLQRTPGEIIRSMRLI
HHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
DAVIDEAIARCFHSYVGERLRELEHLQSSLTLHNEELTRLVTSNEDKLSQLAHELKHPLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AIIGYSDLFLRQQRREITRIDNTVNIEHIERVLRNGRHLLRLINNVLELSRYEAGKIQLQ
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEC
PAPIHIRDLINNVCEMLEPLAAEKNLAILINWVQAPSEIITDGFQLQQIITNLVSNAIRY
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHCCHHHHHCHHHHHHHHHHHHHHHHHC
TESGNISIVCEAIDDQKWAIAVSDTGIGIAEKDQTKIFEPYFRVSSKDRPYLPDSTGLGL
CCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHH
AIVSRLVKLLQGEINLVSKEGLGSTFTVTLPLKVKT
HHHHHHHHHHHCHHHEEECCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]